ElanA.01263.a

Acyl-CoA dehydrogenase, short-chain specific

CENTER ID: ElanA.01263.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.01263.a.B1.GE40591 Full length(ElanA.01263.a) 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.501
UniProt: A0A077EEX9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIQQAARDFA QNELLPGVIE RDDQQKFPAE QVKKMGEMGF LGMMVDPKYG GAGMDSVSYV LAMEEISKVD ASAAVVMSVN NSLVCAGLER FASEEQKVKY LTPLAKGEVI GAFALSEPEA GSDATSQSTT AEDKGDYYLL NGTKNWITNG NNATYYIVIA QTHPEKGHKG INAFIVEKGW EGFIVGKKED KLGIRGSDTH SLMFTDVKVP KENRIGEDGF GFKFAMAVLN GGRIGIASQA LGIASGAYEL ALKYAKERKA FGTEIINHQA VAFKLADMHV NITAARMLCF KAAVEKDAGK DISESGAMAK LFASKTAMDT TIEAVQIHGG YGYVKEYHVE RMMRDAKITQ IYEGTSEIQK IVISRAIAK
NT Sequence
ATGATTCAGC AGGCAGCAAG AGATTTTGCA CAGAACGAAC TTTTACCCGG AGTAATTGAA AGAGACGATC AGCAAAAATT TCCGGCCGAG CAGGTAAAGA AAATGGGGGA AATGGGTTTC CTGGGTATGA TGGTAGATCC TAAATATGGA GGTGCCGGAA TGGACAGTGT TTCTTATGTA TTAGCAATGG AGGAGATCTC TAAGGTAGAT GCTTCTGCAG CTGTTGTCAT GTCGGTAAAT AACTCTTTGG TATGCGCAGG ATTAGAGCGT TTCGCGAGTG AAGAACAAAA AGTAAAATAC CTTACACCTT TAGCAAAAGG AGAGGTAATT GGTGCGTTTG CTTTGTCTGA GCCTGAAGCG GGATCTGACG CTACATCACA AAGTACTACT GCAGAAGATA AAGGAGACTA CTATTTACTG AATGGTACTA AAAACTGGAT TACAAATGGT AATAATGCTA CTTACTATAT TGTAATTGCA CAGACTCACC CGGAAAAAGG TCATAAAGGT ATCAATGCTT TTATAGTAGA AAAAGGCTGG GAAGGTTTTA TAGTTGGTAA AAAAGAAGAT AAATTAGGAA TCAGAGGAAG CGATACACAT TCTTTGATGT TTACTGATGT AAAGGTACCT AAAGAAAACA GAATTGGTGA AGACGGATTC GGTTTCAAAT TTGCAATGGC CGTATTAAAT GGTGGTAGAA TAGGTATTGC TTCTCAGGCT TTAGGTATTG CTTCAGGAGC TTATGAACTG GCACTGAAGT ATGCTAAAGA AAGAAAAGCT TTCGGAACTG AAATTATCAA TCATCAGGCT GTTGCATTTA AATTAGCAGA TATGCATGTG AATATTACTG CTGCAAGAAT GCTATGCTTT AAAGCGGCAG TTGAAAAAGA TGCAGGAAAA GATATCTCTG AAAGTGGGGC TATGGCAAAA TTATTTGCCT CTAAAACTGC TATGGATACT ACGATTGAAG CAGTACAGAT CCACGGTGGA TATGGATATG TAAAAGAGTA CCATGTAGAG CGTATGATGC GTGATGCGAA GATTACTCAG ATCTATGAAG GAACTTCTGA AATTCAGAAA ATCGTTATTT CCAGAGCTAT TGCTAAA
Details for ElanA.01263.a.B1.GE40591
HARVESTED ON: data unavailable
SEQUENCED ON: 10/19/2016
EXPECTED MW: 41kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMI QQAARDFAQN ELLPGVIERD DXXXFPAEQV KXXGEMGFLG MMVDPKYGGA GMDSVSYVLA MEEISKVDAS AAVVMSVNNS LVCAGLERFA SEEQKVKYLT PLAKGEVIGA FALSEPEAGS DATSQSTTAE DKGDYYLLNG TKNWITNGNN ATYYIVIAQT HPEKGHKGIN AFIVEKGWEG FIVGKKEDKL GIRGSDTHSL MFTDVKVPKE NRIGEDGFGF KFAMAVLNGG RIGIASQALG IASGAYELAL KYAKERKAFG TEIINHQAVA FKLADMHVNI TAARMLCFKA AVEKDAGKDI SESGXMAKLF ASXXAMDXTI EAVQIHGGYG YVKEYHVERM MRDAKITQIY EGTXEIQKI
Validated NT Sequence
aacgattttc tgaatttcag nagttccttc atagatctga gtaatcttcg catcacgcat catacgctct acatggtact cttttacata tccatatcca ccgtggatct gtactgcttc aatcgtnnta tccatagcan nnttagaggc aaataatttt gccatnnncc cactttcaga gatatctttt cctgcatctt tttcaactgc cgctttaaag catagcattc ttgcagcagt aatattcaca tgcatatctg ctaatttaaa tgcaacagcc tgatgattga taatttcagt tccgaaagct tttctttctt tagcatactt cagtgccagt tcataagctc ctgaagcaat acctaaagcc tgagaagcaa tacctattct accaccattt aatacggcca ttgcaaattt gaaaccgaat ccgtcttcac caattctgtt ttctttaggt acctttacat cagtaaacat caaagaatgt gtatcgcttc ctctgattcc taatttatct tcttttttac caactataaa accttcccag cctttttcta ctataaaagc attgatacct ttatgacctt tttccgggtg agtctgtgca attacaatat agtaagtagc attattacca tttgtaatcc agtttttagt accattcagt aaatagtagt ctcctttatc ttctgcagta gtactttgtg atgtagcgtc agatcccgct tcaggctcag acaaagcaaa cgcaccaatt acctctcctt ttgctaaagg tgtaaggtat tttacttttt gttcttcact cgcgaaacgc tctaatcctg cgcataccaa agagttattt accgacatga caacagctgc agaagcatct accttagaga tctcctccat tgctaataca taagaaacac tgtccattcc ggcacctcca tatttaggat ctaccatcat acccaggaaa cccatttccc ccannttctt tacctgctcg gccggaaann ntnnntgatc gtctctttca attactccgg gtaaaagttc gttctgtgca aaatctcttg ctgcctgctg aatcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMI QQAARDFAQN ELLPGVIERD DQQKFPAEQV KKMGEMGFLG MMVDPKYGGA GMDSVSYVLA MEEISKVDAS AAVVMSVNNS LVCAGLERFA SEEQKVKYLT PLAKGEVIGA FALSEPEAGS DATSQSTTAE DKGDYYLLNG TKNWITNGNN ATYYIVIAQT HPEKGHKGIN AFIVEKGWEG FIVGKKEDKL GIRGSDTHSL MFTDVKVPKE NRIGEDGFGF KFAMAVLNGG RIGIASQALG IASGAYELAL KYAKERKAFG TEIINHQAVA FKLADMHVNI TAARMLCFKA AVEKDAGKDI SESGAMAKLF ASKTAMDTTI EAVQIHGGYG YVKEYHVERM MRDAKITQIY EGTSEIQKIV ISRAIAK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgattc agcaggcagc aagagatttt gcacagaacg aacttttacc cggagtaatt gaaagagacg atcagcaaaa atttccggcc gagcaggtaa agaaaatggg ggaaatgggt ttcctgggta tgatggtaga tcctaaatat ggaggtgccg gaatggacag tgtttcttat gtattagcaa tggaggagat ctctaaggta gatgcttctg cagctgttgt catgtcggta aataactctt tggtatgcgc aggattagag cgtttcgcga gtgaagaaca aaaagtaaaa taccttacac ctttagcaaa aggagaggta attggtgcgt ttgctttgtc tgagcctgaa gcgggatctg acgctacatc acaaagtact actgcagaag ataaaggaga ctactattta ctgaatggta ctaaaaactg gattacaaat ggtaataatg ctacttacta tattgtaatt gcacagactc acccggaaaa aggtcataaa ggtatcaatg cttttatagt agaaaaaggc tgggaaggtt ttatagttgg taaaaaagaa gataaattag gaatcagagg aagcgataca cattctttga tgtttactga tgtaaaggta cctaaagaaa acagaattgg tgaagacgga ttcggtttca aatttgcaat ggccgtatta aatggtggta gaataggtat tgcttctcag gctttaggta ttgcttcagg agcttatgaa ctggcactga agtatgctaa agaaagaaaa gctttcggaa ctgaaattat caatcatcag gctgttgcat ttaaattagc agatatgcat gtgaatatta ctgctgcaag aatgctatgc tttaaagcgg cagttgaaaa agatgcagga aaagatatct ctgaaagtgg ggctatggca aaattatttg cctctaaaac tgctatggat actacgattg aagcagtaca gatccacggt ggatatggat atgtaaaaga gtaccatgta gagcgtatga tgcgtgatgc gaagattact cagatctatg aaggaacttc tgaaattcag aaaatcgtta tttccagagc tattgctaaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat