ElanA.01178.a

Succinyl-CoA ligase [ADP-forming] subunit alpha

CENTER ID: ElanA.01178.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.01178.a.B1.GE40590 Full length(ElanA.01178.a) 1 291
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.3169
UniProt: A0A077EG78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVLVNKDSK VIVQGFTGNE GTFHAGQMIE YGTNVVGGVT PGKGGSEHLG KPVFNTVAEA VEKAGANVSI IFVPPAFAAD AVMEAADAGI KVIVCITEGI PVADMVKVKD YIQDKDCRLI GPNCPGIITS DEAKIGIMPG FVFKKGRVGI VSKSGTLTYE AADQVVRAGF GVSTAIGIGG DPIIGTTTKE ALELFINDPE TDAVVMIGEI GGQLEGDAAK WYKASGSTKP VVGFIAGQTA PKGRTMGHAG AIVGGDDDTA QAKMKIMAEC GINVVASPAD IGATVAKVLN K
NT Sequence
ATGTCAGTAT TAGTAAACAA AGATTCGAAA GTAATCGTAC AAGGATTTAC CGGTAATGAA GGTACTTTCC ATGCAGGTCA AATGATAGAA TACGGAACAA ATGTAGTAGG TGGTGTAACT CCAGGAAAAG GTGGTTCTGA GCACTTAGGA AAGCCTGTAT TCAACACTGT TGCAGAAGCT GTAGAAAAAG CAGGAGCTAA CGTAAGTATT ATCTTCGTTC CACCGGCATT TGCTGCTGAT GCAGTAATGG AAGCTGCCGA TGCAGGTATC AAAGTAATCG TTTGTATTAC TGAAGGTATT CCTGTAGCTG ACATGGTAAA AGTTAAGGAC TATATTCAGG ATAAAGATTG TCGTTTAATC GGTCCAAACT GCCCTGGTAT CATTACTTCT GACGAGGCTA AAATTGGTAT TATGCCAGGT TTCGTTTTCA AGAAAGGTAG AGTAGGTATC GTATCTAAGT CAGGTACTTT AACATATGAA GCAGCGGATC AGGTTGTAAG AGCTGGATTT GGTGTTTCTA CAGCTATTGG TATTGGTGGT GACCCAATCA TTGGTACTAC TACTAAAGAA GCTTTAGAAT TATTCATCAA TGATCCTGAA ACTGATGCAG TAGTTATGAT CGGTGAGATT GGTGGTCAGT TAGAAGGTGA TGCTGCTAAA TGGTACAAAG CTTCTGGTTC TACTAAGCCA GTTGTAGGTT TCATTGCAGG TCAGACAGCT CCTAAAGGAA GAACAATGGG GCACGCAGGT GCTATCGTAG GTGGTGATGA TGATACAGCT CAGGCTAAAA TGAAGATTAT GGCAGAATGC GGAATCAACG TAGTAGCTTC TCCTGCTGAT ATTGGTGCTA CTGTAGCAAA AGTTTTAAAC AAA
Details for ElanA.01178.a.B1.GE40590
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS VLVNKDSKVI VQGFTGNEGT FHAGQMIEYG TNVVGGVTPG KGGSEHLGKP VFNTVAEAVE KAGANVSIIF VPPAFAADAV MEAADAGIKV IVCITEGIPV ADMVKVKDYI QDKDCRLIGP NCPGIITSDE AKIGIMPGFV FKKGRVGIVS KSGTLTYEAA DQVVRAGFGV STAIGIGGDP IIGTTTKEAL ELFINDPETD AVVMIGEIGG QLEGDAAKWY KASGSTKPVV GFIAGQTAPK GRTMGHAGAI VGGDDDTAQA KMKIMAECGI NVVASXADIG ATXAKVLNK
Validated NT Sequence
tcttactcac ttatttgttt aaaacttttg ctncagtagc accaatatca gcnnnngaag ctactacgtt gattccgcat tctgccataa tcttcatttt agcctgagct gtatcatcat caccacctac gatagcacct gcgtgcccca ttgttcttcc tttaggagct gtctgacctg caatgaaacc tacaactggc ttagtagaac cagaagcttt gtaccattta gcagcatcac cttctaactg accaccaatc tcaccgatca taactactgc atcagtttca ggatcattga tgaataattc taaagcttct ttagtagtag taccaatgat tgggtcacca ccaataccaa tagctgtaga aacaccaaat ccagctctta caacctgatc cgctgcttca tatgttaaag tacctgactt agatacgata cctactctac ctttcttgaa aacgaaacct ggcataatac caattttagc ctcgtcagaa gtaatgatac cagggcagtt tggaccgatt aaacgacaat ctttatcctg aatatagtcc ttaactttta ccatgtcagc tacaggaata ccttcagtaa tacaaacgat tactttgata cctgcatcgg cagcttccat tactgcatca gcagcaaatg ccggtggaac gaagataata cttacgttag ctcctgcttt ttctacagct tctgcaacag tgttgaatac aggctttcct aagtgctcag aaccaccttt tcctggagtt acaccaccta ctacatttgt tccgtattct atcatttgac ctgcatggaa agtaccttca ttaccggtaa atccttgtac gattactttc gaatctttgt ttactaatac tgacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS VLVNKDSKVI VQGFTGNEGT FHAGQMIEYG TNVVGGVTPG KGGSEHLGKP VFNTVAEAVE KAGANVSIIF VPPAFAADAV MEAADAGIKV IVCITEGIPV ADMVKVKDYI QDKDCRLIGP NCPGIITSDE AKIGIMPGFV FKKGRVGIVS KSGTLTYEAA DQVVRAGFGV STAIGIGGDP IIGTTTKEAL ELFINDPETD AVVMIGEIGG QLEGDAAKWY KASGSTKPVV GFIAGQTAPK GRTMGHAGAI VGGDDDTAQA KMKIMAECGI NVVASPADIG ATVAKVLNK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcag tattagtaaa caaagattcg aaagtaatcg tacaaggatt taccggtaat gaaggtactt tccatgcagg tcaaatgata gaatacggaa caaatgtagt aggtggtgta actccaggaa aaggtggttc tgagcactta ggaaagcctg tattcaacac tgttgcagaa gctgtagaaa aagcaggagc taacgtaagt attatcttcg ttccaccggc atttgctgct gatgcagtaa tggaagctgc cgatgcaggt atcaaagtaa tcgtttgtat tactgaaggt attcctgtag ctgacatggt aaaagttaag gactatattc aggataaaga ttgtcgttta atcggtccaa actgccctgg tatcattact tctgacgagg ctaaaattgg tattatgcca ggtttcgttt tcaagaaagg tagagtaggt atcgtatcta agtcaggtac tttaacatat gaagcagcgg atcaggttgt aagagctgga tttggtgttt ctacagctat tggtattggt ggtgacccaa tcattggtac tactactaaa gaagctttag aattattcat caatgatcct gaaactgatg cagtagttat gatcggtgag attggtggtc agttagaagg tgatgctgct aaatggtaca aagcttctgg ttctactaag ccagttgtag gtttcattgc aggtcagaca gctcctaaag gaagaacaat ggggcacgca ggtgctatcg taggtggtga tgatgataca gctcaggcta aaatgaagat tatggcagaa tgcggaatca acgtagtagc ttctcctgct gatattggtg ctactgtagc aaaagtttta aacaaatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t