ElanA.00906.a

Cystathionine gamma-lyase

CENTER ID: ElanA.00906.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00906.a.B1.GE40585 Full length(ElanA.00906.a) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00906.a.B1.PW38246 Full length(ElanA.00906.a) 1 382

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.2119
UniProt: A0A077EBZ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFNTKVIHG NQHAEPHTGS VNVPVFLTST FAQKSPGQLR AGYEYSRGAN PTRQALEDAL ASIENGARGL AFGSGLAAID CVLKLLNPGD EVIAVDDLYG GTYRMFTRLF EKYQLKFTFV NFDDVSKIAE LINDKTKLIW LETPTNPLMK LVDIKAVADL IQGKDILLAV DNTFASPYLQ QPLDLGADII MHSATKYLGG HSDVVAGALV AKTEELGEKL HFIQFASGGI LGPHDSYLVL RGIKTLALRM QRHSENGIKV AQFLEKHSAI EHVFYPGLES HPQNDLAKKQ MKDFGGMVSF TLKSGKKEDS VKFLENLRVF TLAESLGGVE SLANHPAMMT HASIPAEKRA ELGITDDLVR LSAGIEDIED LLQDLEQALN AI
NT Sequence
ATGAAATTCA ATACTAAAGT CATTCATGGA AACCAACATG CAGAGCCCCA TACAGGTTCT GTAAATGTAC CGGTATTCCT TACTTCAACT TTCGCACAAA AAAGTCCTGG ACAACTGCGT GCAGGATATG AATATTCCCG TGGTGCAAAC CCAACACGTC AGGCATTAGA AGATGCTCTG GCAAGTATAG AGAACGGAGC CAGAGGTTTA GCTTTCGGTT CAGGCCTTGC AGCTATAGAT TGCGTACTGA AATTATTAAA TCCGGGTGAT GAAGTTATTG CTGTAGACGA TCTCTACGGT GGTACTTACA GAATGTTTAC CCGCCTTTTC GAAAAATATC AGTTAAAGTT TACGTTCGTT AACTTCGATG ACGTTTCAAA AATAGCTGAA CTTATTAATG ATAAAACAAA GCTAATCTGG TTAGAGACAC CAACGAATCC TTTAATGAAG CTAGTAGACA TTAAAGCCGT TGCAGACCTT ATTCAGGGGA AAGATATTCT TTTAGCTGTA GACAATACTT TTGCCTCACC ATACCTTCAA CAGCCTTTAG ACCTAGGTGC GGATATCATC ATGCATTCTG CTACGAAATA TCTTGGAGGT CACTCTGATG TTGTTGCAGG AGCTTTAGTT GCTAAAACAG AAGAATTAGG AGAAAAACTA CACTTTATTC AGTTTGCATC CGGAGGTATT TTAGGTCCAC ATGATTCTTA CTTAGTACTA AGAGGTATTA AAACTTTAGC ATTGAGAATG CAAAGACATT CTGAAAACGG AATCAAAGTT GCTCAATTCC TGGAAAAACA TTCGGCTATT GAACATGTAT TTTATCCGGG ATTAGAAAGC CATCCACAAA ATGATTTAGC TAAAAAACAA ATGAAAGATT TTGGCGGAAT GGTTTCTTTT ACCCTCAAAT CTGGTAAAAA AGAAGATTCT GTTAAGTTCC TTGAAAATCT TCGTGTTTTC ACATTAGCAG AATCACTTGG TGGTGTAGAG TCTTTAGCAA ATCACCCAGC TATGATGACA CACGCATCCA TTCCTGCTGA AAAACGTGCT GAGTTAGGTA TCACTGATGA TCTTGTACGT CTTAGTGCAG GTATTGAGGA TATTGAAGAT CTATTACAGG ATCTGGAGCA AGCGCTGAAC GCTATA
Details for ElanA.00906.a.B1.GE40585
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 43kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMK FNTKVIHGNQ HAEPHTGSVN VPVFLTSTFA QKSPGQLRAG YEYSRGANPT RQALEDALAS IENGARGLAF GSGLAAIDCV LKLLNPGDEV IAVDDLYGGT YRMFTRLFEK YQLKFTFVNF DDVSKIAELI NDKTKLIWLE TPTNPLMKLV DIKAVADLIQ GKDILLAVDN TFASPYLQQP LDLGADIIMH SATKYLGGHS DVVAGALVAK TEELGEKLHF IQFASGGILG PHDSYLVLRG IKTLALRMQR HSENGIKVAQ FLEKHSAIEH VFYPGLESHP QNDLAKKQMK DFGGMVSFTL KSGKKEDSVK FLENLRVFTL AESLGGVESL ANHPAMMTHA SIPAEKRAEL GITDDLVRLS AGIEDIEDLL Q
Validated NT Sequence
atcctgtaat agatcttcaa tatcctcaat acctgcacta agacgtacaa gatcatcagt gatacctaac tcagcacgtt tttcagcagg aatggatgcg tgtgtcatca tagctgggtg atttgctaaa gactctacac caccaagtga ttctgctaat gtgaaaacac gaagattttc aaggaactta acagaatctt cttttttacc agatttgagg gtaaaagaaa ccattccgcc aaaatctttc atttgttttt tagctaaatc attttgtgga tggctttcta atcccggata aaatacatgt tcaatagccg aatgtttttc caggaattga gcaactttga ttccgttttc agaatgtctt tgcattctca atgctaaagt tttaatacct cttagtacta agtaagaatc atgtggacct aaaatacctc cggatgcaaa ctgaataaag tgtagttttt ctcctaattc ttctgtttta gcaactaaag ctcctgcaac aacatcagag tgacctccaa gatatttcgt agcagaatgc atgatgatat ccgcacctag gtctaaaggc tgttgaaggt atggtgaggc aaaagtattg tctacagcta aaagaatatc tttcccctga ataaggtctg caacggcttt aatgtctact agcttcatta aaggattcgt tggtgtctct aaccagatta gctttgtttt atcattaata agttcagcta tttttgaaac gtcatcgaag ttaacgaacg taaactttaa ctgatatttt tcgaaaaggc gggtaaacat tctgtaagta ccaccgtaga gatcgtctac agcaataact tcatcacccg gatttaataa tttcagtacg caatctatag ctgcaaggcc tgaaccgaaa gctaaacctc tggctccgtt ctctatactt gccagagcat cttctaatgc ctgacgtgtt gggtttgcac cacgggaata ttcatatcct gcacgcagtt gtccaggact tttttgtgcg aaagttgaag taaggaatac cggtacattt acagaacctg tatggggctc tgcatgttgg tttccatgaa tgactttagt attgaatttc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMK FNTKVIHGNQ HAEPHTGSVN VPVFLTSTFA QKSPGQLRAG YEYSRGANPT RQALEDALAS IENGARGLAF GSGLAAIDCV LKLLNPGDEV IAVDDLYGGT YRMFTRLFEK YQLKFTFVNF DDVSKIAELI NDKTKLIWLE TPTNPLMKLV DIKAVADLIQ GKDILLAVDN TFASPYLQQP LDLGADIIMH SATKYLGGHS DVVAGALVAK TEELGEKLHF IQFASGGILG PHDSYLVLRG IKTLALRMQR HSENGIKVAQ FLEKHSAIEH VFYPGLESHP QNDLAKKQMK DFGGMVSFTL KSGKKEDSVK FLENLRVFTL AESLGGVESL ANHPAMMTHA SIPAEKRAEL GITDDLVRLS AGIEDIEDLL QDLEQALNAI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat tcaatactaa agtcattcat ggaaaccaac atgcagagcc ccatacaggt tctgtaaatg taccggtatt ccttacttca actttcgcac aaaaaagtcc tggacaactg cgtgcaggat atgaatattc ccgtggtgca aacccaacac gtcaggcatt agaagatgct ctggcaagta tagagaacgg agccagaggt ttagctttcg gttcaggcct tgcagctata gattgcgtac tgaaattatt aaatccgggt gatgaagtta ttgctgtaga cgatctctac ggtggtactt acagaatgtt tacccgcctt ttcgaaaaat atcagttaaa gtttacgttc gttaacttcg atgacgtttc aaaaatagct gaacttatta atgataaaac aaagctaatc tggttagaga caccaacgaa tcctttaatg aagctagtag acattaaagc cgttgcagac cttattcagg ggaaagatat tcttttagct gtagacaata cttttgcctc accatacctt caacagcctt tagacctagg tgcggatatc atcatgcatt ctgctacgaa atatcttgga ggtcactctg atgttgttgc aggagcttta gttgctaaaa cagaagaatt aggagaaaaa ctacacttta ttcagtttgc atccggaggt attttaggtc cacatgattc ttacttagta ctaagaggta ttaaaacttt agcattgaga atgcaaagac attctgaaaa cggaatcaaa gttgctcaat tcctggaaaa acattcggct attgaacatg tattttatcc gggattagaa agccatccac aaaatgattt agctaaaaaa caaatgaaag attttggcgg aatggtttct tttaccctca aatctggtaa aaaagaagat tctgttaagt tccttgaaaa tcttcgtgtt ttcacattag cagaatcact tggtggtgta gagtctttag caaatcaccc agctatgatg acacacgcat ccattcctgc tgaaaaacgt gctgagttag gtatcactga tgatcttgta cgtcttagtg caggtattga ggatattgaa gatctattac aggatctgga gcaagcgctg aacgctatat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for ElanA.00906.a.B1.PW38246
PURIFICATION DATe: 4/11/2017
CONCENTRATION: 41mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 12
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK FNTKVIHGNQ HAEPHTGSVN VPVFLTSTFA QKSPGQLRAG YEYSRGANPT RQALEDALAS IENGARGLAF GSGLAAIDCV LKLLNPGDEV IAVDDLYGGT YRMFTRLFEK YQLKFTFVNF DDVSKIAELI NDKTKLIWLE TPTNPLMKLV DIKAVADLIQ GKDILLAVDN TFASPYLQQP LDLGADIIMH SATKYLGGHS DVVAGALVAK TEELGEKLHF IQFASGGILG PHDSYLVLRG IKTLALRMQR HSENGIKVAQ FLEKHSAIEH VFYPGLESHP QNDLAKKQMK DFGGMVSFTL KSGKKEDSVK FLENLRVFTL AESLGGVESL ANHPAMMTHA SIPAEKRAEL GITDDLVRLS AGIEDIEDLL Q
Validated NT Sequence
atcctgtaat agatcttcaa tatcctcaat acctgcacta agacgtacaa gatcatcagt gatacctaac tcagcacgtt tttcagcagg aatggatgcg tgtgtcatca tagctgggtg atttgctaaa gactctacac caccaagtga ttctgctaat gtgaaaacac gaagattttc aaggaactta acagaatctt cttttttacc agatttgagg gtaaaagaaa ccattccgcc aaaatctttc atttgttttt tagctaaatc attttgtgga tggctttcta atcccggata aaatacatgt tcaatagccg aatgtttttc caggaattga gcaactttga ttccgttttc agaatgtctt tgcattctca atgctaaagt tttaatacct cttagtacta agtaagaatc atgtggacct aaaatacctc cggatgcaaa ctgaataaag tgtagttttt ctcctaattc ttctgtttta gcaactaaag ctcctgcaac aacatcagag tgacctccaa gatatttcgt agcagaatgc atgatgatat ccgcacctag gtctaaaggc tgttgaaggt atggtgaggc aaaagtattg tctacagcta aaagaatatc tttcccctga ataaggtctg caacggcttt aatgtctact agcttcatta aaggattcgt tggtgtctct aaccagatta gctttgtttt atcattaata agttcagcta tttttgaaac gtcatcgaag ttaacgaacg taaactttaa ctgatatttt tcgaaaaggc gggtaaacat tctgtaagta ccaccgtaga gatcgtctac agcaataact tcatcacccg gatttaataa tttcagtacg caatctatag ctgcaaggcc tgaaccgaaa gctaaacctc tggctccgtt ctctatactt gccagagcat cttctaatgc ctgacgtgtt gggtttgcac cacgggaata ttcatatcct gcacgcagtt gtccaggact tttttgtgcg aaagttgaag taaggaatac cggtacattt acagaacctg tatggggctc tgcatgttgg tttccatgaa tgactttagt attgaatttc atatggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMK FNTKVIHGNQ HAEPHTGSVN VPVFLTSTFA QKSPGQLRAG YEYSRGANPT RQALEDALAS IENGARGLAF GSGLAAIDCV LKLLNPGDEV IAVDDLYGGT YRMFTRLFEK YQLKFTFVNF DDVSKIAELI NDKTKLIWLE TPTNPLMKLV DIKAVADLIQ GKDILLAVDN TFASPYLQQP LDLGADIIMH SATKYLGGHS DVVAGALVAK TEELGEKLHF IQFASGGILG PHDSYLVLRG IKTLALRMQR HSENGIKVAQ FLEKHSAIEH VFYPGLESHP QNDLAKKQMK DFGGMVSFTL KSGKKEDSVK FLENLRVFTL AESLGGVESL ANHPAMMTHA SIPAEKRAEL GITDDLVRLS AGIEDIEDLL QDLEQALNAI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAAT TCAATACTAA AGTCATTCAT GGAAACCAAC ATGCAGAGCC CCATACAGGT TCTGTAAATG TACCGGTATT CCTTACTTCA ACTTTCGCAC AAAAAAGTCC TGGACAACTG CGTGCAGGAT ATGAATATTC CCGTGGTGCA AACCCAACAC GTCAGGCATT AGAAGATGCT CTGGCAAGTA TAGAGAACGG AGCCAGAGGT TTAGCTTTCG GTTCAGGCCT TGCAGCTATA GATTGCGTAC TGAAATTATT AAATCCGGGT GATGAAGTTA TTGCTGTAGA CGATCTCTAC GGTGGTACTT ACAGAATGTT TACCCGCCTT TTCGAAAAAT ATCAGTTAAA GTTTACGTTC GTTAACTTCG ATGACGTTTC AAAAATAGCT GAACTTATTA ATGATAAAAC AAAGCTAATC TGGTTAGAGA CACCAACGAA TCCTTTAATG AAGCTAGTAG ACATTAAAGC CGTTGCAGAC CTTATTCAGG GGAAAGATAT TCTTTTAGCT GTAGACAATA CTTTTGCCTC ACCATACCTT CAACAGCCTT TAGACCTAGG TGCGGATATC ATCATGCATT CTGCTACGAA ATATCTTGGA GGTCACTCTG ATGTTGTTGC AGGAGCTTTA GTTGCTAAAA CAGAAGAATT AGGAGAAAAA CTACACTTTA TTCAGTTTGC ATCCGGAGGT ATTTTAGGTC CACATGATTC TTACTTAGTA CTAAGAGGTA TTAAAACTTT AGCATTGAGA ATGCAAAGAC ATTCTGAAAA CGGAATCAAA GTTGCTCAAT TCCTGGAAAA ACATTCGGCT ATTGAACATG TATTTTATCC GGGATTAGAA AGCCATCCAC AAAATGATTT AGCTAAAAAA CAAATGAAAG ATTTTGGCGG AATGGTTTCT TTTACCCTCA AATCTGGTAA AAAAGAAGAT TCTGTTAAGT TCCTTGAAAA TCTTCGTGTT TTCACATTAG CAGAATCACT TGGTGGTGTA GAGTCTTTAG CAAATCACCC AGCTATGATG ACACACGCAT CCATTCCTGC TGAAAAACGT GCTGAGTTAG GTATCACTGA TGATCTTGTA CGTCTTAGTG CAGGTATTGA GGATATTGAA GATCTATTAC AGGATCTGGA GCAAGCGCTG AACGCTATAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT