ElanA.00855.a

Glyceraldehyde-3-phosphate dehydrogenase

CENTER ID: ElanA.00855.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00855.a.B1.GE40584 Full length(ElanA.00855.a) 1 334
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00855.a.B1.PW37982 Full length(ElanA.00855.a) 1 334

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.1763
UniProt: A0A077EG40

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKIKVGING FGRIGRLVFK AMTERDNIEV VGINDLIDAE YMAYMLKYDS VHGQFKGEIS VEGNDLVVNG NRIRVTAEKD PNNLKWNEVG AEYIVESTGL FLTKESAQAH INAGAKKVIL SAPPKDDTPM FVMGVNHNEL TDDIKILSNA SCTTNCLAPL AKVIHDNFGI AEGLMTTVHA ATATQKTVDG PSMKDWRGGR SALNNIIPSS TGAAKAVGKV IPSLNGKLTG MSFRVPTADV SVVDLTVRLD KATTYDEICA TIKAAAEGEL KGILGYTEDA VVSQDFVGEK RTSVFDKDAG IMLSPTFVKL VSWYDNETGY SNKLTDMLIH SASL
NT Sequence
ATGTCAAAAA TCAAAGTAGG TATCAACGGA TTTGGTAGAA TCGGACGTCT TGTATTCAAA GCAATGACTG AAAGAGACAA TATTGAGGTA GTTGGTATCA ACGACCTTAT CGATGCAGAG TACATGGCTT ACATGCTAAA ATACGACTCT GTACACGGGC AATTTAAAGG AGAGATTTCT GTAGAAGGTA ACGACCTTGT TGTAAACGGA AACAGAATTC GTGTAACTGC TGAAAAAGAT CCTAACAACC TTAAATGGAA TGAAGTTGGG GCTGAATATA TCGTAGAATC TACTGGTTTA TTCTTAACTA AAGAAAGTGC ACAGGCTCAC ATTAACGCTG GTGCTAAAAA AGTAATTCTT TCTGCACCTC CGAAAGATGA TACTCCAATG TTTGTAATGG GTGTTAACCA CAACGAACTA ACTGATGATA TCAAAATCTT ATCAAATGCA TCTTGTACAA CTAACTGTTT AGCTCCTTTA GCTAAAGTTA TCCACGATAA CTTTGGTATC GCTGAAGGTC TAATGACTAC TGTACACGCT GCTACTGCTA CTCAGAAAAC TGTTGACGGT CCTTCAATGA AAGACTGGAG AGGCGGACGT TCTGCACTGA ACAACATTAT CCCTTCTTCT ACTGGTGCTG CTAAGGCTGT AGGTAAAGTT ATCCCTTCTC TTAACGGAAA ACTTACCGGT ATGTCTTTCA GAGTACCAAC GGCTGACGTT TCTGTAGTAG ACTTAACTGT AAGATTAGAT AAAGCTACAA CTTACGATGA GATTTGTGCT ACTATTAAAG CTGCTGCTGA AGGTGAATTA AAAGGAATCC TTGGTTATAC TGAAGATGCT GTTGTATCTC AGGATTTCGT AGGAGAAAAG AGAACTTCAG TATTCGATAA AGATGCTGGT ATTATGTTAT CTCCAACATT CGTAAAATTA GTTTCTTGGT ATGACAACGA AACTGGTTAC TCTAACAAGT TAACAGATAT GTTAATCCAC TCTGCTTCTT TA
Details for ElanA.00855.a.B1.GE40584
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 37kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMS KIKVGINGFG RIGRLVFKAM TERDNIEVVG INDLIDAEYM AYMLKYDSVH GQFKGEISVE GNDLVVNGNR IRVTAEKDPN NLKWNEVGAE YIVESTGLFL TKESAQAHIN AGAKKVILSA PPKDDTPMFV MGVNHNELTD DIKILSNASC TTNCLAPLAK VIHDNFGIAE GLMTTVHAAT ATQKTVDGPS MKDWRGGRSA LNNIIPSSTG AAKAVGKVIP SLNGKLTGMS FRVPTADVSV VDLTVRLDKA TTYDEICATI KAAAEGELKG ILGYTEDAVV SQDFVGEKRT SVFDKDAGIM LSPTFVKLVS WYDNETGYSN KLT
Validated NT Sequence
tctgttaact tgttagagta accagtttcg ttgtcatacc aagaaactaa ttttacgaat gttggagata acataatacc agcatcttta tcgaatactg aagttctctt ttctcctacg aaatcctgag atacaacagc atcttcagta taaccaagga ttccttttaa ttcaccttca gcagcagctt taatagtagc acaaatctca tcgtaagttg tagctttatc taatcttaca gttaagtcta ctacagaaac gtcagccgtt ggtactctga aagacatacc ggtaagtttt ccgttaagag aagggataac tttacctaca gccttagcag caccagtaga agaagggata atgttgttca gtgcagaacg tccgcctctc cagtctttca ttgaaggacc gtcaacagtt ttctgagtag cagtagcagc gtgtacagta gtcattagac cttcagcgat accaaagtta tcgtggataa ctttagctaa aggagctaaa cagttagttg tacaagatgc atttgataag attttgatat catcagttag ttcgttgtgg ttaacaccca ttacaaacat tggagtatca tctttcggag gtgcagaaag aattactttt ttagcaccag cgttaatgtg agcctgtgca ctttctttag ttaagaataa accagtagat tctacgatat attcagcccc aacttcattc catttaaggt tgttaggatc tttttcagca gttacacgaa ttctgtttcc gtttacaaca aggtcgttac cttctacaga aatctctcct ttaaattgcc cgtgtacaga gtcgtatttt agcatgtaag ccatgtactc tgcatcgata aggtcgttga taccaactac ctcaatattg tctctttcag tcattgcttt gaatacaaga cgtccgattc taccaaatcc gttgatacct actttgattt ttgacatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHMS KIKVGINGFG RIGRLVFKAM TERDNIEVVG INDLIDAEYM AYMLKYDSVH GQFKGEISVE GNDLVVNGNR IRVTAEKDPN NLKWNEVGAE YIVESTGLFL TKESAQAHIN AGAKKVILSA PPKDDTPMFV MGVNHNELTD DIKILSNASC TTNCLAPLAK VIHDNFGIAE GLMTTVHAAT ATQKTVDGPS MKDWRGGRSA LNNIIPSSTG AAKAVGKVIP SLNGKLTGMS FRVPTADVSV VDLTVRLDKA TTYDEICATI KAAAEGELKG ILGYTEDAVV SQDFVGEKRT SVFDKDAGIM LSPTFVKLVS WYDNETGYSN KLTDMLIHSA SL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcaa aaatcaaagt aggtatcaac ggatttggta gaatcggacg tcttgtattc aaagcaatga ctgaaagaga caatattgag gtagttggta tcaacgacct tatcgatgca gagtacatgg cttacatgct aaaatacgac tctgtacacg ggcaatttaa aggagagatt tctgtagaag gtaacgacct tgttgtaaac ggaaacagaa ttcgtgtaac tgctgaaaaa gatcctaaca accttaaatg gaatgaagtt ggggctgaat atatcgtaga atctactggt ttattcttaa ctaaagaaag tgcacaggct cacattaacg ctggtgctaa aaaagtaatt ctttctgcac ctccgaaaga tgatactcca atgtttgtaa tgggtgttaa ccacaacgaa ctaactgatg atatcaaaat cttatcaaat gcatcttgta caactaactg tttagctcct ttagctaaag ttatccacga taactttggt atcgctgaag gtctaatgac tactgtacac gctgctactg ctactcagaa aactgttgac ggtccttcaa tgaaagactg gagaggcgga cgttctgcac tgaacaacat tatcccttct tctactggtg ctgctaaggc tgtaggtaaa gttatccctt ctcttaacgg aaaacttacc ggtatgtctt tcagagtacc aacggctgac gtttctgtag tagacttaac tgtaagatta gataaagcta caacttacga tgagatttgt gctactatta aagctgctgc tgaaggtgaa ttaaaaggaa tccttggtta tactgaagat gctgttgtat ctcaggattt cgtaggagaa aagagaactt cagtattcga taaagatgct ggtattatgt tatctccaac attcgtaaaa ttagtttctt ggtatgacaa cgaaactggt tactctaaca agttaacaga tatgttaatc cactctgctt ctttatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for ElanA.00855.a.B1.PW37982
PURIFICATION DATe: 12/5/2016
CONCENTRATION: 50mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS KIKVGINGFG RIGRLVFKAM TERDNIEVVG INDLIDAEYM AYMLKYDSVH GQFKGEISVE GNDLVVNGNR IRVTAEKDPN NLKWNEVGAE YIVESTGLFL TKESAQAHIN AGAKKVILSA PPKDDTPMFV MGVNHNELTD DIKILSNASC TTNCLAPLAK VIHDNFGIAE GLMTTVHAAT ATQKTVDGPS MKDWRGGRSA LNNIIPSSTG AAKAVGKVIP SLNGKLTGMS FRVPTADVSV VDLTVRLDKA TTYDEICATI KAAAEGELKG ILGYTEDAVV SQDFVGEKRT SVFDKDAGIM LSPTFVKLVS WYDNETGYSN KLT
Validated NT Sequence
tctgttaact tgttagagta accagtttcg ttgtcatacc aagaaactaa ttttacgaat gttggagata acataatacc agcatcttta tcgaatactg aagttctctt ttctcctacg aaatcctgag atacaacagc atcttcagta taaccaagga ttccttttaa ttcaccttca gcagcagctt taatagtagc acaaatctca tcgtaagttg tagctttatc taatcttaca gttaagtcta ctacagaaac gtcagccgtt ggtactctga aagacatacc ggtaagtttt ccgttaagag aagggataac tttacctaca gccttagcag caccagtaga agaagggata atgttgttca gtgcagaacg tccgcctctc cagtctttca ttgaaggacc gtcaacagtt ttctgagtag cagtagcagc gtgtacagta gtcattagac cttcagcgat accaaagtta tcgtggataa ctttagctaa aggagctaaa cagttagttg tacaagatgc atttgataag attttgatat catcagttag ttcgttgtgg ttaacaccca ttacaaacat tggagtatca tctttcggag gtgcagaaag aattactttt ttagcaccag cgttaatgtg agcctgtgca ctttctttag ttaagaataa accagtagat tctacgatat attcagcccc aacttcattc catttaaggt tgttaggatc tttttcagca gttacacgaa ttctgtttcc gtttacaaca aggtcgttac cttctacaga aatctctcct ttaaattgcc cgtgtacaga gtcgtatttt agcatgtaag ccatgtactc tgcatcgata aggtcgttga taccaactac ctcaatattg tctctttcag tcattgcttt gaatacaaga cgtccgattc taccaaatcc gttgatacct actttgattt ttgacatatg gtggtggtgg tggtgagcca t
Expressed Protein Sequence
MAHHHHHHMS KIKVGINGFG RIGRLVFKAM TERDNIEVVG INDLIDAEYM AYMLKYDSVH GQFKGEISVE GNDLVVNGNR IRVTAEKDPN NLKWNEVGAE YIVESTGLFL TKESAQAHIN AGAKKVILSA PPKDDTPMFV MGVNHNELTD DIKILSNASC TTNCLAPLAK VIHDNFGIAE GLMTTVHAAT ATQKTVDGPS MKDWRGGRSA LNNIIPSSTG AAKAVGKVIP SLNGKLTGMS FRVPTADVSV VDLTVRLDKA TTYDEICATI KAAAEGELKG ILGYTEDAVV SQDFVGEKRT SVFDKDAGIM LSPTFVKLVS WYDNETGYSN KLTDMLIHSA SL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCAA AAATCAAAGT AGGTATCAAC GGATTTGGTA GAATCGGACG TCTTGTATTC AAAGCAATGA CTGAAAGAGA CAATATTGAG GTAGTTGGTA TCAACGACCT TATCGATGCA GAGTACATGG CTTACATGCT AAAATACGAC TCTGTACACG GGCAATTTAA AGGAGAGATT TCTGTAGAAG GTAACGACCT TGTTGTAAAC GGAAACAGAA TTCGTGTAAC TGCTGAAAAA GATCCTAACA ACCTTAAATG GAATGAAGTT GGGGCTGAAT ATATCGTAGA ATCTACTGGT TTATTCTTAA CTAAAGAAAG TGCACAGGCT CACATTAACG CTGGTGCTAA AAAAGTAATT CTTTCTGCAC CTCCGAAAGA TGATACTCCA ATGTTTGTAA TGGGTGTTAA CCACAACGAA CTAACTGATG ATATCAAAAT CTTATCAAAT GCATCTTGTA CAACTAACTG TTTAGCTCCT TTAGCTAAAG TTATCCACGA TAACTTTGGT ATCGCTGAAG GTCTAATGAC TACTGTACAC GCTGCTACTG CTACTCAGAA AACTGTTGAC GGTCCTTCAA TGAAAGACTG GAGAGGCGGA CGTTCTGCAC TGAACAACAT TATCCCTTCT TCTACTGGTG CTGCTAAGGC TGTAGGTAAA GTTATCCCTT CTCTTAACGG AAAACTTACC GGTATGTCTT TCAGAGTACC AACGGCTGAC GTTTCTGTAG TAGACTTAAC TGTAAGATTA GATAAAGCTA CAACTTACGA TGAGATTTGT GCTACTATTA AAGCTGCTGC TGAAGGTGAA TTAAAAGGAA TCCTTGGTTA TACTGAAGAT GCTGTTGTAT CTCAGGATTT CGTAGGAGAA AAGAGAACTT CAGTATTCGA TAAAGATGCT GGTATTATGT TATCTCCAAC ATTCGTAAAA TTAGTTTCTT GGTATGACAA CGAAACTGGT TACTCTAACA AGTTAACAGA TATGTTAATC CACTCTGCTT CTTTATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT