ElanA.00825.a

Asparagine--tRNA ligase

CENTER ID: ElanA.00825.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00825.a.B1.GE41087 Full length(ElanA.00825.a) 1 482
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00825.a.B1.PW38258 Full length(ElanA.00825.a) 1 482

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.3422
UniProt: A0A077EHQ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHKQTIKEVL ENYKKFLHHD ITVYGWVRAF RSNRFIALND GSTINNLQIV VDFENFDENL IKNINTASSL KIVGEVVESQ GAGQTVEIIA KKIIVLGDNF TEELQNTILQ PKKHSLEKLR EQAHLRFRTN LFGAVFRVRH AVSFAIHSFF NDRQFFYLNT PVITGADAEG AGEMFGVTNF DLDNIPRNED GAIDYTQDFF GRKTNLTVSG QLEGETAAMG LGRIYTFGPT FRAENSNTTR HLAEFWMVEP EVAFNNLEDN IDLAEDFLKY VIQYVLDKCK DDLEFLDKRF AEEQKQKPEK ERAKEGLIEK LENVVAKRFK RVSYTEAIDI LLNSKENKKG KFVYPVEKWG ADLQSEHERY LVEKHFECPV VLFDYPAEIK AFYMRLNEDN KTVAAMDVLF PGIGEIIGGS QREERLDVLK KKMDDMHVDQ EELWWYLDTR KFGSVPHSGF GLGLERLVLF VTGMTNIRDV IPFPRTPKSA EF
NT Sequence
ATGCATAAGC AGACAATTAA AGAAGTTTTA GAAAATTACA AAAAATTCTT GCATCACGAT ATCACCGTGT ATGGGTGGGT ACGTGCGTTT CGTTCAAACA GGTTTATTGC CTTAAACGAT GGTTCAACAA TCAATAATTT ACAAATCGTA GTTGATTTCG AAAATTTTGA TGAGAATCTT ATCAAGAATA TAAATACGGC TTCTTCATTG AAAATTGTTG GTGAAGTGGT GGAAAGTCAG GGAGCAGGAC AAACGGTTGA AATTATTGCT AAGAAAATTA TTGTACTTGG AGATAACTTT ACTGAAGAAT TGCAGAATAC TATACTTCAG CCAAAGAAGC ATAGCTTAGA AAAGCTTCGT GAACAAGCTC ACCTAAGATT CAGAACTAAT TTGTTTGGTG CTGTTTTCAG AGTGCGTCAT GCTGTTAGTT TTGCAATTCA CTCGTTCTTC AACGACAGAC AGTTTTTCTA TCTAAATACA CCTGTAATTA CCGGAGCGGA TGCAGAAGGT GCAGGAGAAA TGTTTGGTGT TACCAATTTT GATTTGGATA ATATCCCAAG AAATGAAGAC GGTGCTATAG ACTATACACA GGATTTCTTC GGAAGAAAGA CCAATCTTAC CGTTTCTGGA CAGTTAGAAG GAGAAACTGC TGCAATGGGA TTAGGAAGAA TTTATACATT CGGACCTACT TTCCGTGCCG AAAACTCTAA TACAACACGT CACCTTGCAG AATTCTGGAT GGTGGAGCCT GAAGTAGCTT TTAATAATCT TGAAGATAAT ATTGACCTTG CTGAAGATTT CTTAAAGTAT GTAATTCAGT ATGTTTTAGA CAAATGTAAG GACGATTTAG AATTCCTGGA TAAGCGTTTT GCTGAAGAGC AAAAACAAAA GCCAGAAAAG GAAAGAGCAA AAGAAGGTCT TATTGAAAAG CTGGAAAATG TAGTTGCGAA GAGATTTAAA CGTGTAAGCT ATACTGAAGC AATCGATATT CTTCTGAACT CGAAAGAAAA TAAAAAAGGA AAATTTGTAT ATCCTGTTGA AAAATGGGGT GCAGACCTTC AGTCTGAACA TGAAAGATAC CTTGTTGAAA AGCATTTCGA ATGTCCTGTT GTATTATTCG ATTATCCTGC AGAAATTAAA GCATTCTACA TGCGTCTGAA TGAAGATAAC AAAACTGTAG CTGCTATGGA TGTACTTTTC CCTGGTATTG GTGAGATTAT CGGAGGTTCT CAGAGAGAAG AGAGACTAGA TGTTCTGAAG AAGAAAATGG ATGATATGCA TGTAGATCAG GAAGAACTTT GGTGGTATTT AGATACCCGT AAGTTTGGTT CTGTTCCGCA TTCAGGATTT GGTTTGGGAC TGGAAAGATT AGTTCTTTTT GTTACCGGAA TGACCAACAT TCGTGATGTG ATTCCTTTTC CAAGAACGCC TAAGAGCGCA GAATTC
Details for ElanA.00825.a.B1.GE41087
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMH KQTIKEVLEN YKKFLHHDIT VYGWVRAFRS NRFIALNDGS TINNLQIVVD FENFDENLIK NINTASSLKI VGEVVESQGA GQTVEIIAKK IIVLGDNFTE ELQNTILQPK KHSLEKLREQ AHLRFRTNLF GAVFRVRHAV SFAIHSFFND RQFFYLNTPV ITGADAEGAG EMFGVTNFDL DNIPRNEDGA IDYTQDFFGR KTNLTVSGQL EGETAAMGLG RIYTFGPTFR AENSNTTRHL AEFWMVEPEV AFNNLEDNID LAEDFLKYVI QYVLDKCKDD LEFLDKRFAE EQKQKPEKER AKEGLIEKLE NVVAKRFKRV SYTEAIDILL NSKENKKGKF VYPVEKWGAD LQSEHERYLV EKHFECPVVL FDYPAEIKAF YMRLNEDNKT VAAMDVLFPG IGEIIGGSQR EERLDVLKKK MDDMHVDQEE LWWYLDTRKF GSVPHSGFGL GLERLVLFVT GMTNIRDVIP FP
Validated NT Sequence
tcttggaaaa ggaatcacat cacgaatgtt ggtcattccg gtaacaaaaa gaactaatct ttccagtccc aaaccaaatc ctgaatgcgg aacagaacca aacttacggg tatctaaata ccaccaaagt tcttcctgat ctacatgcat atcatccatt ttcttcttca gaacatctag tctctcttct ctctgagaac ctccgataat ctcaccaata ccagggaaaa gtacatccat agcagctaca gttttgttat cttcattcag acgcatgtag aatgctttaa tttctgcagg ataatcgaat aatacaacag gacattcgaa atgcttttca acaaggtatc tttcatgttc agactgaagg tctgcacccc atttttcaac aggatataca aattttcctt ttttattttc tttcgagttc agaagaatat cgattgcttc agtatagctt acacgtttaa atctcttcgc aactacattt tccagctttt caataagacc ttcttttgct ctttcctttt ctggcttttg tttttgctct tcagcaaaac gcttatccag gaattctaaa tcgtccttac atttgtctaa aacatactga attacatact ttaagaaatc ttcagcaagg tcaatattat cttcaagatt attaaaagct acttcaggct ccaccatcca gaattctgca aggtgacgtg ttgtattaga gttttcggca cggaaagtag gtccgaatgt ataaattctt cctaatccca ttgcagcagt ttctccttct aactgtccag aaacggtaag attggtcttt cttccgaaga aatcctgtgt atagtctata gcaccgtctt catttcttgg gatattatcc aaatcaaaat tggtaacacc aaacatttct cctgcacctt ctgcatccgc tccggtaatt acaggtgtat ttagatagaa aaactgtctg tcgttgaaga acgagtgaat tgcaaaacta acagcatgac gcactctgaa aacagcacca aacaaattag ttctgaatct taggtgagct tgttcacgaa gcttttctaa gctatgcttc tttggctgaa gtatagtatt ctgcaattct tcagtaaagt tatctccaag tacaataatt ttcttagcaa taatttcaac cgtttgtcct gctccctgac tttccaccac ttcaccaaca attttcaatg aagaagccgt atttatattc ttgataagat tctcatcaaa attttcgaaa tcaactacga tttgtaaatt attgattgtt gaaccatcgt ttaaggcaat aaacctgttt gaacgaaacg cacgtaccca cccatacacg gtgatatcgt gatgcaagaa ttttttgtaa ttttctaaaa cttctttaat tgtctgctta tgcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMH KQTIKEVLEN YKKFLHHDIT VYGWVRAFRS NRFIALNDGS TINNLQIVVD FENFDENLIK NINTASSLKI VGEVVESQGA GQTVEIIAKK IIVLGDNFTE ELQNTILQPK KHSLEKLREQ AHLRFRTNLF GAVFRVRHAV SFAIHSFFND RQFFYLNTPV ITGADAEGAG EMFGVTNFDL DNIPRNEDGA IDYTQDFFGR KTNLTVSGQL EGETAAMGLG RIYTFGPTFR AENSNTTRHL AEFWMVEPEV AFNNLEDNID LAEDFLKYVI QYVLDKCKDD LEFLDKRFAE EQKQKPEKER AKEGLIEKLE NVVAKRFKRV SYTEAIDILL NSKENKKGKF VYPVEKWGAD LQSEHERYLV EKHFECPVVL FDYPAEIKAF YMRLNEDNKT VAAMDVLFPG IGEIIGGSQR EERLDVLKKK MDDMHVDQEE LWWYLDTRKF GSVPHSGFGL GLERLVLFVT GMTNIRDVIP FPRTPKSAEF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata agcagacaat taaagaagtt ttagaaaatt acaaaaaatt cttgcatcac gatatcaccg tgtatgggtg ggtacgtgcg tttcgttcaa acaggtttat tgccttaaac gatggttcaa caatcaataa tttacaaatc gtagttgatt tcgaaaattt tgatgagaat cttatcaaga atataaatac ggcttcttca ttgaaaattg ttggtgaagt ggtggaaagt cagggagcag gacaaacggt tgaaattatt gctaagaaaa ttattgtact tggagataac tttactgaag aattgcagaa tactatactt cagccaaaga agcatagctt agaaaagctt cgtgaacaag ctcacctaag attcagaact aatttgtttg gtgctgtttt cagagtgcgt catgctgtta gttttgcaat tcactcgttc ttcaacgaca gacagttttt ctatctaaat acacctgtaa ttaccggagc ggatgcagaa ggtgcaggag aaatgtttgg tgttaccaat tttgatttgg ataatatccc aagaaatgaa gacggtgcta tagactatac acaggatttc ttcggaagaa agaccaatct taccgtttct ggacagttag aaggagaaac tgctgcaatg ggattaggaa gaatttatac attcggacct actttccgtg ccgaaaactc taatacaaca cgtcaccttg cagaattctg gatggtggag cctgaagtag cttttaataa tcttgaagat aatattgacc ttgctgaaga tttcttaaag tatgtaattc agtatgtttt agacaaatgt aaggacgatt tagaattcct ggataagcgt tttgctgaag agcaaaaaca aaagccagaa aaggaaagag caaaagaagg tcttattgaa aagctggaaa atgtagttgc gaagagattt aaacgtgtaa gctatactga agcaatcgat attcttctga actcgaaaga aaataaaaaa ggaaaatttg tatatcctgt tgaaaaatgg ggtgcagacc ttcagtctga acatgaaaga taccttgttg aaaagcattt cgaatgtcct gttgtattat tcgattatcc tgcagaaatt aaagcattct acatgcgtct gaatgaagat aacaaaactg tagctgctat ggatgtactt ttccctggta ttggtgagat tatcggaggt tctcagagag aagagagact agatgttctg aagaagaaaa tggatgatat gcatgtagat caggaagaac tttggtggta tttagatacc cgtaagtttg gttctgttcc gcattcagga tttggtttgg gactggaaag attagttctt tttgttaccg gaatgaccaa cattcgtgat gtgattcctt ttccaagaac gcctaagagc gcagaattct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for ElanA.00825.a.B1.PW38258
PURIFICATION DATe: 4/25/2017
CONCENTRATION: 25mg/ml
OBSERVED MW: 58kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 35
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMH KQTIKEVLEN YKKFLHHDIT VYGWVRAFRS NRFIALNDGS TINNLQIVVD FENFDENLIK NINTASSLKI VGEVVESQGA GQTVEIIAKK IIVLGDNFTE ELQNTILQPK KHSLEKLREQ AHLRFRTNLF GAVFRVRHAV SFAIHSFFND RQFFYLNTPV ITGADAEGAG EMFGVTNFDL DNIPRNEDGA IDYTQDFFGR KTNLTVSGQL EGETAAMGLG RIYTFGPTFR AENSNTTRHL AEFWMVEPEV AFNNLEDNID LAEDFLKYVI QYVLDKCKDD LEFLDKRFAE EQKQKPEKER AKEGLIEKLE NVVAKRFKRV SYTEAIDILL NSKENKKGKF VYPVEKWGAD LQSEHERYLV EKHFECPVVL FDYPAEIKAF YMRLNEDNKT VAAMDVLFPG IGEIIGGSQR EERLDVLKKK MDDMHVDQEE LWWYLDTRKF GSVPHSGFGL GLERLVLFVT GMTNIRDVIP FP
Validated NT Sequence
tcttggaaaa ggaatcacat cacgaatgtt ggtcattccg gtaacaaaaa gaactaatct ttccagtccc aaaccaaatc ctgaatgcgg aacagaacca aacttacggg tatctaaata ccaccaaagt tcttcctgat ctacatgcat atcatccatt ttcttcttca gaacatctag tctctcttct ctctgagaac ctccgataat ctcaccaata ccagggaaaa gtacatccat agcagctaca gttttgttat cttcattcag acgcatgtag aatgctttaa tttctgcagg ataatcgaat aatacaacag gacattcgaa atgcttttca acaaggtatc tttcatgttc agactgaagg tctgcacccc atttttcaac aggatataca aattttcctt ttttattttc tttcgagttc agaagaatat cgattgcttc agtatagctt acacgtttaa atctcttcgc aactacattt tccagctttt caataagacc ttcttttgct ctttcctttt ctggcttttg tttttgctct tcagcaaaac gcttatccag gaattctaaa tcgtccttac atttgtctaa aacatactga attacatact ttaagaaatc ttcagcaagg tcaatattat cttcaagatt attaaaagct acttcaggct ccaccatcca gaattctgca aggtgacgtg ttgtattaga gttttcggca cggaaagtag gtccgaatgt ataaattctt cctaatccca ttgcagcagt ttctccttct aactgtccag aaacggtaag attggtcttt cttccgaaga aatcctgtgt atagtctata gcaccgtctt catttcttgg gatattatcc aaatcaaaat tggtaacacc aaacatttct cctgcacctt ctgcatccgc tccggtaatt acaggtgtat ttagatagaa aaactgtctg tcgttgaaga acgagtgaat tgcaaaacta acagcatgac gcactctgaa aacagcacca aacaaattag ttctgaatct taggtgagct tgttcacgaa gcttttctaa gctatgcttc tttggctgaa gtatagtatt ctgcaattct tcagtaaagt tatctccaag tacaataatt ttcttagcaa taatttcaac cgtttgtcct gctccctgac tttccaccac ttcaccaaca attttcaatg aagaagccgt atttatattc ttgataagat tctcatcaaa attttcgaaa tcaactacga tttgtaaatt attgattgtt gaaccatcgt ttaaggcaat aaacctgttt gaacgaaacg cacgtaccca cccatacacg gtgatatcgt gatgcaagaa ttttttgtaa ttttctaaaa cttctttaat tgtctgctta tgcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMH KQTIKEVLEN YKKFLHHDIT VYGWVRAFRS NRFIALNDGS TINNLQIVVD FENFDENLIK NINTASSLKI VGEVVESQGA GQTVEIIAKK IIVLGDNFTE ELQNTILQPK KHSLEKLREQ AHLRFRTNLF GAVFRVRHAV SFAIHSFFND RQFFYLNTPV ITGADAEGAG EMFGVTNFDL DNIPRNEDGA IDYTQDFFGR KTNLTVSGQL EGETAAMGLG RIYTFGPTFR AENSNTTRHL AEFWMVEPEV AFNNLEDNID LAEDFLKYVI QYVLDKCKDD LEFLDKRFAE EQKQKPEKER AKEGLIEKLE NVVAKRFKRV SYTEAIDILL NSKENKKGKF VYPVEKWGAD LQSEHERYLV EKHFECPVVL FDYPAEIKAF YMRLNEDNKT VAAMDVLFPG IGEIIGGSQR EERLDVLKKK MDDMHVDQEE LWWYLDTRKF GSVPHSGFGL GLERLVLFVT GMTNIRDVIP FPRTPKSAEF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCATA AGCAGACAAT TAAAGAAGTT TTAGAAAATT ACAAAAAATT CTTGCATCAC GATATCACCG TGTATGGGTG GGTACGTGCG TTTCGTTCAA ACAGGTTTAT TGCCTTAAAC GATGGTTCAA CAATCAATAA TTTACAAATC GTAGTTGATT TCGAAAATTT TGATGAGAAT CTTATCAAGA ATATAAATAC GGCTTCTTCA TTGAAAATTG TTGGTGAAGT GGTGGAAAGT CAGGGAGCAG GACAAACGGT TGAAATTATT GCTAAGAAAA TTATTGTACT TGGAGATAAC TTTACTGAAG AATTGCAGAA TACTATACTT CAGCCAAAGA AGCATAGCTT AGAAAAGCTT CGTGAACAAG CTCACCTAAG ATTCAGAACT AATTTGTTTG GTGCTGTTTT CAGAGTGCGT CATGCTGTTA GTTTTGCAAT TCACTCGTTC TTCAACGACA GACAGTTTTT CTATCTAAAT ACACCTGTAA TTACCGGAGC GGATGCAGAA GGTGCAGGAG AAATGTTTGG TGTTACCAAT TTTGATTTGG ATAATATCCC AAGAAATGAA GACGGTGCTA TAGACTATAC ACAGGATTTC TTCGGAAGAA AGACCAATCT TACCGTTTCT GGACAGTTAG AAGGAGAAAC TGCTGCAATG GGATTAGGAA GAATTTATAC ATTCGGACCT ACTTTCCGTG CCGAAAACTC TAATACAACA CGTCACCTTG CAGAATTCTG GATGGTGGAG CCTGAAGTAG CTTTTAATAA TCTTGAAGAT AATATTGACC TTGCTGAAGA TTTCTTAAAG TATGTAATTC AGTATGTTTT AGACAAATGT AAGGACGATT TAGAATTCCT GGATAAGCGT TTTGCTGAAG AGCAAAAACA AAAGCCAGAA AAGGAAAGAG CAAAAGAAGG TCTTATTGAA AAGCTGGAAA ATGTAGTTGC GAAGAGATTT AAACGTGTAA GCTATACTGA AGCAATCGAT ATTCTTCTGA ACTCGAAAGA AAATAAAAAA GGAAAATTTG TATATCCTGT TGAAAAATGG GGTGCAGACC TTCAGTCTGA ACATGAAAGA TACCTTGTTG AAAAGCATTT CGAATGTCCT GTTGTATTAT TCGATTATCC TGCAGAAATT AAAGCATTCT ACATGCGTCT GAATGAAGAT AACAAAACTG TAGCTGCTAT GGATGTACTT TTCCCTGGTA TTGGTGAGAT TATCGGAGGT TCTCAGAGAG AAGAGAGACT AGATGTTCTG AAGAAGAAAA TGGATGATAT GCATGTAGAT CAGGAAGAAC TTTGGTGGTA TTTAGATACC CGTAAGTTTG GTTCTGTTCC GCATTCAGGA TTTGGTTTGG GACTGGAAAG ATTAGTTCTT TTTGTTACCG GAATGACCAA CATTCGTGAT GTGATTCCTT TTCCAAGAAC GCCTAAGAGC GCAGAATTCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT