SSGCID: Target Page

ElanA.00612.a

Lysine--tRNA ligase

CENTER ID:	ElanA.00612.a
ORGANISM:	Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.00612.a.B1.GE41084	Full length(ElanA.00612.a)		1	567

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.00612.a.B1.PW38253	Full length(ElanA.00612.a)		1	567

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1338011.4.peg.584
UniProt:	A0A077EHZ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQQLTEQEII RREKLQQLRQ MGIEPYPAAE FEVNTNTQEI KDKYEDGKKV KLAGRLMSVR IMGKASFAEL QDSEGRVQIY VSRDDVSSTE EAVEYNTVFK KLLDIGDFIG IEGYLFKTQV GEISVHVTKF TLLSKTLRPL PVVKTDEDGK VHDAFVDPEL RYRMRYVDLA VNPHVKDIFI KRTKLFNAMR GFFNDKGYFE VETPVLQSIP GGAAARPFIT HHNALDIPLY LRIANELYLK RLIVGGFDGV YEFSKNFRNE GMDRTHNPEF TAMEIYVAYK DYNWMMDFTE KLLEHCAIAV NGTSKATFGE HEIDFKAPYP RVSMTEAIQK YTGYDITGKT EEELRAFAKS IGLEVDETMG KGKLIDEIFG EKCEGNFIQP TFITDYPVEM SPLTKKHRSQ EGLTERFELM VCGKEIANAY SELNDPIDQR ERFEEQLRLS EKGDDEAGQF IDEDFLRALE YGMPPTSGLG IGMDRLIMFL TNNPSIQEVL FFPQMRPEKT EPKLELNDDE KAIIEIVSTN EEATALAEVK DKSQLSGKKW DKAIKNLTKN NLVKVEKVEE VLLIKMV

NT Sequence

ATGCAACAAC TTACAGAGCA AGAAATCATC CGCAGAGAAA AACTGCAACA GCTGAGACAG ATGGGGATCG AGCCTTATCC GGCAGCGGAA TTTGAAGTAA ATACAAATAC GCAGGAGATA AAGGACAAGT ACGAGGATGG TAAGAAGGTG AAGTTGGCAG GACGCTTGAT GAGTGTTCGT ATTATGGGGA AAGCTTCATT CGCAGAGCTT CAGGATAGCG AAGGGCGTGT ACAGATTTAT GTATCACGTG ATGATGTTTC CAGTACAGAA GAAGCAGTGG AGTATAACAC CGTTTTCAAA AAACTTTTAG ATATTGGTGA TTTTATCGGA ATCGAAGGTT ATCTGTTCAA AACACAGGTA GGAGAGATCT CCGTACATGT AACCAAATTT ACCCTTTTAT CAAAAACATT ACGTCCGCTA CCAGTTGTAA AAACTGATGA AGACGGAAAA GTTCACGATG CATTTGTAGA TCCGGAATTA CGTTACCGTA TGCGTTATGT AGATTTAGCC GTGAATCCGC ATGTTAAGGA TATTTTCATT AAGAGAACTA AATTATTCAA TGCTATGCGT GGTTTCTTCA ACGACAAAGG ATACTTTGAG GTTGAAACTC CGGTATTACA ATCTATCCCT GGTGGAGCAG CTGCAAGACC CTTTATTACG CATCATAATG CATTGGATAT TCCTTTATAC CTTCGTATTG CAAACGAATT ATACCTAAAG AGACTAATCG TTGGAGGTTT TGATGGTGTT TATGAGTTTT CTAAAAACTT CCGTAACGAA GGAATGGACA GAACTCACAA TCCGGAATTT ACAGCTATGG AAATTTATGT AGCTTACAAA GACTACAACT GGATGATGGA TTTCACAGAG AAATTACTAG AGCACTGTGC TATTGCAGTA AACGGAACTT CTAAAGCAAC GTTTGGTGAA CATGAAATTG ATTTCAAAGC TCCATATCCA AGAGTATCTA TGACTGAAGC TATTCAGAAA TATACAGGAT ATGATATTAC CGGAAAAACT GAAGAAGAGC TTCGTGCTTT TGCTAAATCT ATCGGCTTAG AGGTCGACGA AACAATGGGG AAAGGAAAAC TTATCGATGA AATCTTCGGT GAGAAATGTG AAGGTAACTT TATTCAGCCA ACTTTCATTA CAGACTATCC TGTAGAGATG TCTCCGCTAA CTAAAAAACA CAGAAGTCAG GAAGGCCTAA CCGAGCGTTT CGAACTTATG GTTTGCGGTA AGGAGATTGC TAATGCTTAT TCAGAGCTTA ACGATCCAAT CGACCAGAGA GAGCGTTTTG AAGAGCAACT TAGACTTTCT GAGAAAGGTG ATGATGAAGC AGGTCAGTTT ATCGACGAAG ACTTCCTTAG AGCTTTAGAA TATGGTATGC CGCCAACTTC AGGTTTAGGA ATTGGTATGG ACAGATTAAT CATGTTCCTT ACTAATAATC CGTCTATTCA GGAGGTATTA TTCTTCCCGC AAATGCGTCC GGAGAAAACA GAGCCCAAAT TGGAACTTAA TGACGATGAA AAAGCGATTA TCGAAATCGT AAGCACAAAT GAAGAAGCAA CTGCATTAGC TGAAGTTAAA GATAAAAGCC AGTTATCCGG TAAGAAATGG GATAAAGCTA TTAAAAACCT TACGAAAAAC AACTTAGTGA AAGTTGAAAA AGTAGAAGAG GTTCTATTGA TTAAAATGGT C

Details for ElanA.00612.a.B1.GE41084

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	66kDa
OBSERVED MW:	66kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMQ QLTEQEIIRR EKLQQLRQMG IEPYPAAEFE VNTNTQEIKD KYEDGKKVKL AGRLMSVRIM GKASFAELQD SEGRVQIYVS RDDVSSTEEA VEYNTVFKKL LDIGDFIGIE GYLFKTQVGE ISVHVTKFTL LSKTLRPLPV VKTDEDGKVH DAFVDPELRY RMRYVDLAVN PHVKDIFIKR TKLFNAMRGF FNDKGYFEVE TPVLQSIPGG AAARPFITHH NALDIPLYLR IANELYLKRL IVGGFDGVYE FSKNFRNEGM DRTHNPEFTA MEIYVAYKDY NWMMDFTEKL LEHCAIAVNG TSKATFGEHE IDFKAPYPRV SMTEAIQKYT GYDITGKTEE ELRAFAKSIG LEVDETMGKG KLIDEIFGEK CEGNFIQPTF ITDYPVEMSP LTKKHRSQEG LTERFELMVC GKEIANAYSE LNDPIDQRER FEEQLRLSEK GDDEAGQFID EDFLRALEYG MPPTSGLGIG MDRLIMFLTN NPSIQEVLFF PQMRPEKTEP KLELNDDEKA IIEIVSTNEE ATALAEVKDK SQLSGKKWDK AIKNLTKNNL VKVEKVEEVL LIKMV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac aacttacaga gcaagaaatc atccgcagag aaaaactgca acagctgaga cagatgggga tcgagcctta tccggcagcg gaatttgaag taaatacaaa tacgcaggag ataaaggaca agtacgagga tggtaagaag gtgaagttgg caggacgctt gatgagtgtt cgtattatgg ggaaagcttc attcgcagag cttcaggata gcgaagggcg tgtacagatt tatgtatcac gtgatgatgt ttccagtaca gaagaagcag tggagtataa caccgttttc aaaaaacttt tagatattgg tgattttatc ggaatcgaag gttatctgtt caaaacacag gtaggagaga tctccgtaca tgtaaccaaa tttacccttt tatcaaaaac attacgtccg ctaccagttg taaaaactga tgaagacgga aaagttcacg atgcatttgt agatccggaa ttacgttacc gtatgcgtta tgtagattta gccgtgaatc cgcatgttaa ggatattttc attaagagaa ctaaattatt caatgctatg cgtggtttct tcaacgacaa aggatacttt gaggttgaaa ctccggtatt acaatctatc cctggtggag cagctgcaag accctttatt acgcatcata atgcattgga tattccttta taccttcgta ttgcaaacga attataccta aagagactaa tcgttggagg ttttgatggt gtttatgagt tttctaaaaa cttccgtaac gaaggaatgg acagaactca caatccggaa tttacagcta tggaaattta tgtagcttac aaagactaca actggatgat ggatttcaca gagaaattac tagagcactg tgctattgca gtaaacggaa cttctaaagc aacgtttggt gaacatgaaa ttgatttcaa agctccatat ccaagagtat ctatgactga agctattcag aaatatacag gatatgatat taccggaaaa actgaagaag agcttcgtgc ttttgctaaa tctatcggct tagaggtcga cgaaacaatg gggaaaggaa aacttatcga tgaaatcttc ggtgagaaat gtgaaggtaa ctttattcag ccaactttca ttacagacta tcctgtagag atgtctccgc taactaaaaa acacagaagt caggaaggcc taaccgagcg tttcgaactt atggtttgcg gtaaggagat tgctaatgct tattcagagc ttaacgatcc aatcgaccag agagagcgtt ttgaagagca acttagactt tctgagaaag gtgatgatga agcaggtcag tttatcgacg aagacttcct tagagcttta gaatatggta tgccgccaac ttcaggttta ggaattggta tggacagatt aatcatgttc cttactaata atccgtctat tcaggaggta ttattcttcc cgcaaatgcg tccggagaaa acagagccca aattggaact taatgacgat gaaaaagcga ttatcgaaat cgtaagcaca aatgaagaag caactgcatt agctgaagtt aaagataaaa gccagttatc cggtaagaaa tgggataaag ctattaaaaa ccttacgaaa aacaacttag tgaaagttga aaaagtagaa gaggttctat tgattaaaat ggtctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for ElanA.00612.a.B1.PW38253

PURIFICATION DATe:	4/11/2017
CONCENTRATION:	25mg/ml
OBSERVED MW:	73kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	17
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAC AACTTACAGA GCAAGAAATC ATCCGCAGAG AAAAACTGCA ACAGCTGAGA CAGATGGGGA TCGAGCCTTA TCCGGCAGCG GAATTTGAAG TAAATACAAA TACGCAGGAG ATAAAGGACA AGTACGAGGA TGGTAAGAAG GTGAAGTTGG CAGGACGCTT GATGAGTGTT CGTATTATGG GGAAAGCTTC ATTCGCAGAG CTTCAGGATA GCGAAGGGCG TGTACAGATT TATGTATCAC GTGATGATGT TTCCAGTACA GAAGAAGCAG TGGAGTATAA CACCGTTTTC AAAAAACTTT TAGATATTGG TGATTTTATC GGAATCGAAG GTTATCTGTT CAAAACACAG GTAGGAGAGA TCTCCGTACA TGTAACCAAA TTTACCCTTT TATCAAAAAC ATTACGTCCG CTACCAGTTG TAAAAACTGA TGAAGACGGA AAAGTTCACG ATGCATTTGT AGATCCGGAA TTACGTTACC GTATGCGTTA TGTAGATTTA GCCGTGAATC CGCATGTTAA GGATATTTTC ATTAAGAGAA CTAAATTATT CAATGCTATG CGTGGTTTCT TCAACGACAA AGGATACTTT GAGGTTGAAA CTCCGGTATT ACAATCTATC CCTGGTGGAG CAGCTGCAAG ACCCTTTATT ACGCATCATA ATGCATTGGA TATTCCTTTA TACCTTCGTA TTGCAAACGA ATTATACCTA AAGAGACTAA TCGTTGGAGG TTTTGATGGT GTTTATGAGT TTTCTAAAAA CTTCCGTAAC GAAGGAATGG ACAGAACTCA CAATCCGGAA TTTACAGCTA TGGAAATTTA TGTAGCTTAC AAAGACTACA ACTGGATGAT GGATTTCACA GAGAAATTAC TAGAGCACTG TGCTATTGCA GTAAACGGAA CTTCTAAAGC AACGTTTGGT GAACATGAAA TTGATTTCAA AGCTCCATAT CCAAGAGTAT CTATGACTGA AGCTATTCAG AAATATACAG GATATGATAT TACCGGAAAA ACTGAAGAAG AGCTTCGTGC TTTTGCTAAA TCTATCGGCT TAGAGGTCGA CGAAACAATG GGGAAAGGAA AACTTATCGA TGAAATCTTC GGTGAGAAAT GTGAAGGTAA CTTTATTCAG CCAACTTTCA TTACAGACTA TCCTGTAGAG ATGTCTCCGC TAACTAAAAA ACACAGAAGT CAGGAAGGCC TAACCGAGCG TTTCGAACTT ATGGTTTGCG GTAAGGAGAT TGCTAATGCT TATTCAGAGC TTAACGATCC AATCGACCAG AGAGAGCGTT TTGAAGAGCA ACTTAGACTT TCTGAGAAAG GTGATGATGA AGCAGGTCAG TTTATCGACG AAGACTTCCT TAGAGCTTTA GAATATGGTA TGCCGCCAAC TTCAGGTTTA GGAATTGGTA TGGACAGATT AATCATGTTC CTTACTAATA ATCCGTCTAT TCAGGAGGTA TTATTCTTCC CGCAAATGCG TCCGGAGAAA ACAGAGCCCA AATTGGAACT TAATGACGAT GAAAAAGCGA TTATCGAAAT CGTAAGCACA AATGAAGAAG CAACTGCATT AGCTGAAGTT AAAGATAAAA GCCAGTTATC CGGTAAGAAA TGGGATAAAG CTATTAAAAA CCTTACGAAA AACAACTTAG TGAAAGTTGA AAAAGTAGAA GAGGTTCTAT TGATTAAAAT GGTCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT