ElanA.00567.a

Queuine tRNA-ribosyltransferase

CENTER ID: ElanA.00567.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00567.a.B1.GE40581 Full length(ElanA.00567.a) 1 378
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00567.a.B1.PW38277 Full length(ElanA.00567.a) 1 378

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.2544
UniProt: A0A077ECU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKFFEVEQF SAKGKARAGV ITTDHGKIQT PIFMPVGTVA TVKTVHQREL KDDIKAQIIL GNTYHLYLRP GMDVMQNAGG LHKFMNWNGP ILTDSGGFQV FSLASSRKMT EEGVKFKSHI DGSYHFISPE VSMEIQRKIG ADIFMAFDEC TPYPCEYNQA KVSMELTHRW LKRCIEWTEN NPEYYGHKQR LFPIVQGSVY SDLRKASAEV IAEAGAEGNA IGGLSVGEPE EEMYRITDEV TDILPKDKPR YLMGVGTPWN ILESIGNGID MMDCVMPTRN ARNGMLFTWG GVINIKNEKW KNDFSPLDEL GASYVDQEYT KAYVRHLFSA REYLGKQIAS IHNLAFYLDL VKVAREHILA GDFYEWKDSI IPQLKSRM
NT Sequence
ATGGAGAAGT TTTTTGAAGT AGAACAATTT TCAGCAAAAG GTAAAGCCAG AGCTGGTGTT ATTACAACAG ATCATGGTAA AATACAGACA CCTATTTTTA TGCCGGTGGG TACCGTAGCA ACAGTAAAAA CTGTTCATCA GAGAGAATTG AAAGATGATA TTAAGGCTCA GATAATCTTA GGAAATACAT ACCACCTGTA CCTGAGACCA GGTATGGATG TTATGCAGAA TGCAGGCGGA TTACATAAAT TTATGAACTG GAACGGTCCT ATCCTTACGG ATAGTGGTGG TTTTCAGGTT TTCTCATTGG CTTCCAGCCG GAAAATGACT GAAGAAGGTG TGAAATTCAA ATCTCATATC GACGGAAGTT ATCATTTTAT TTCACCTGAA GTTTCTATGG AAATTCAGCG TAAAATTGGT GCAGATATCT TTATGGCTTT TGACGAATGT ACTCCATATC CATGTGAATA CAATCAGGCA AAAGTTTCTA TGGAACTTAC CCACAGATGG CTGAAGAGAT GTATTGAATG GACGGAAAAT AATCCTGAAT ATTACGGACA CAAACAAAGA TTGTTTCCGA TCGTTCAGGG ATCTGTATAT TCGGATCTCA GAAAAGCTTC CGCTGAAGTT ATTGCTGAGG CAGGAGCTGA AGGAAATGCT ATTGGAGGTC TTTCTGTTGG TGAACCGGAA GAGGAAATGT ACAGAATTAC AGATGAAGTA ACAGATATTC TTCCTAAAGA TAAGCCTCGT TATCTGATGG GGGTTGGAAC ACCATGGAAT ATTCTGGAAT CTATAGGTAA TGGTATTGAT ATGATGGATT GTGTAATGCC TACCCGGAAT GCCAGAAATG GTATGTTGTT CACATGGGGT GGTGTTATCA ATATCAAAAA TGAAAAATGG AAGAATGATT TTTCTCCGTT AGACGAACTT GGTGCATCAT ATGTAGATCA GGAATATACA AAAGCATATG TAAGACACCT GTTCTCAGCA AGAGAATACT TAGGAAAGCA GATTGCTTCT ATTCATAATC TTGCTTTTTA TTTAGATTTG GTAAAAGTCG CAAGAGAACA TATCTTAGCA GGAGATTTCT ATGAATGGAA AGATAGCATT ATTCCACAAC TAAAAAGCAG AATG
Details for ElanA.00567.a.B1.GE40581
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHME KFFEVEQFSA KGKARAGVIT TDHGKIQTPI FMPVGTVATV KTVHQRELKD DIKAQIILGN TYHLYLRPGM DVMQNAGGLH KFMNWNGPIL TDSGGFQVFS LASSRKMTEE GVKFKSHIDG SYHFISPEVS MEIQRKIGAD IFMAFDECTP YPCEYNQAKV SMELTHRWLK RCIEWTENNP EYYGHKQRLF PIVQGSVYSD LRKASAEVIA EAGAEGNAIG GLSVGEPEEE MYRITDEVTD ILPKDKPRYL MGVGTPWNIL ESIGNGIDMM DCVMPTRNAR NGMLFTWGGV INIKNEKWKN DFSPLDELGA SYVDQEYTKA YVRHLFSARE YLGKQIASIH NLAFYLDLVK VAREHILAGD FYEWKDSI
Validated NT Sequence
taatgctatc tttccattca tagaaatctc ctgctaagat atgttctctt gcgactttta ccaaatctaa ataaaaagca agattatgaa tagaagcaat ctgctttcct aagtattctc ttgctgagaa caggtgtctt acatatgctt ttgtatattc ctgatctaca tatgatgcac caagttcgtc taacggagaa aaatcattct tccatttttc atttttgata ttgataacac caccccatgt gaacaacata ccatttctgg cattccgggt aggcattaca caatccatca tatcaatacc attacctata gattccagaa tattccatgg tgttccaacc cccatcagat aacgaggctt atctttagga agaatatctg ttacttcatc tgtaattctg tacatttcct cttccggttc accaacagaa agacctccaa tagcatttcc ttcagctcct gcctcagcaa taacttcagc ggaagctttt ctgagatccg aatatacaga tccctgaacg atcggaaaca atctttgttt gtgtccgtaa tattcaggat tattttccgt ccattcaata catctcttca gccatctgtg ggtaagttcc atagaaactt ttgcctgatt gtattcacat ggatatggag tacattcgtc aaaagccata aagatatctg caccaatttt acgctgaatt tccatagaaa cttcaggtga aataaaatga taacttccgt cgatatgaga tttgaatttc acaccttctt cagtcatttt ccggctggaa gccaatgaga aaacctgaaa accaccacta tccgtaagga taggaccgtt ccagttcata aatttatgta atccgcctgc attctgcata acatccatac ctggtctcag gtacaggtgg tatgtatttc ctaagattat ctgagcctta atatcatctt tcaattctct ctgatgaaca gtttttactg ttgcnacggt acccaccggc ataaaaatag gtgtctgtat tttaccatga tctgttgtaa taacaccagc tctggcttta ccttttgctg aaaattgttc tacttcaaaa aacttctcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHME KFFEVEQFSA KGKARAGVIT TDHGKIQTPI FMPVGTVATV KTVHQRELKD DIKAQIILGN TYHLYLRPGM DVMQNAGGLH KFMNWNGPIL TDSGGFQVFS LASSRKMTEE GVKFKSHIDG SYHFISPEVS MEIQRKIGAD IFMAFDECTP YPCEYNQAKV SMELTHRWLK RCIEWTENNP EYYGHKQRLF PIVQGSVYSD LRKASAEVIA EAGAEGNAIG GLSVGEPEEE MYRITDEVTD ILPKDKPRYL MGVGTPWNIL ESIGNGIDMM DCVMPTRNAR NGMLFTWGGV INIKNEKWKN DFSPLDELGA SYVDQEYTKA YVRHLFSARE YLGKQIASIH NLAFYLDLVK VAREHILAGD FYEWKDSIIP QLKSRM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaga agttttttga agtagaacaa ttttcagcaa aaggtaaagc cagagctggt gttattacaa cagatcatgg taaaatacag acacctattt ttatgccggt gggtaccgta gcaacagtaa aaactgttca tcagagagaa ttgaaagatg atattaaggc tcagataatc ttaggaaata cataccacct gtacctgaga ccaggtatgg atgttatgca gaatgcaggc ggattacata aatttatgaa ctggaacggt cctatcctta cggatagtgg tggttttcag gttttctcat tggcttccag ccggaaaatg actgaagaag gtgtgaaatt caaatctcat atcgacggaa gttatcattt tatttcacct gaagtttcta tggaaattca gcgtaaaatt ggtgcagata tctttatggc ttttgacgaa tgtactccat atccatgtga atacaatcag gcaaaagttt ctatggaact tacccacaga tggctgaaga gatgtattga atggacggaa aataatcctg aatattacgg acacaaacaa agattgtttc cgatcgttca gggatctgta tattcggatc tcagaaaagc ttccgctgaa gttattgctg aggcaggagc tgaaggaaat gctattggag gtctttctgt tggtgaaccg gaagaggaaa tgtacagaat tacagatgaa gtaacagata ttcttcctaa agataagcct cgttatctga tgggggttgg aacaccatgg aatattctgg aatctatagg taatggtatt gatatgatgg attgtgtaat gcctacccgg aatgccagaa atggtatgtt gttcacatgg ggtggtgtta tcaatatcaa aaatgaaaaa tggaagaatg atttttctcc gttagacgaa cttggtgcat catatgtaga tcaggaatat acaaaagcat atgtaagaca cctgttctca gcaagagaat acttaggaaa gcagattgct tctattcata atcttgcttt ttatttagat ttggtaaaag tcgcaagaga acatatctta gcaggagatt tctatgaatg gaaagatagc attattccac aactaaaaag cagaatgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for ElanA.00567.a.B1.PW38277
PURIFICATION DATe: 5/17/2017
CONCENTRATION: 25mg/ml
OBSERVED MW: 41kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHME KFFEVEQFSA KGKARAGVIT TDHGKIQTPI FMPVGTVATV KTVHQRELKD DIKAQIILGN TYHLYLRPGM DVMQNAGGLH KFMNWNGPIL TDSGGFQVFS LASSRKMTEE GVKFKSHIDG SYHFISPEVS MEIQRKIGAD IFMAFDECTP YPCEYNQAKV SMELTHRWLK RCIEWTENNP EYYGHKQRLF PIVQGSVYSD LRKASAEVIA EAGAEGNAIG GLSVGEPEEE MYRITDEVTD ILPKDKPRYL MGVGTPWNIL ESIGNGIDMM DCVMPTRNAR NGMLFTWGGV INIKNEKWKN DFSPLDELGA SYVDQEYTKA YVRHLFSARE YLGKQIASIH NLAFYLDLVK VAREHILAGD FYEWKDSI
Validated NT Sequence
taatgctatc tttccattca tagaaatctc ctgctaagat atgttctctt gcgactttta ccaaatctaa ataaaaagca agattatgaa tagaagcaat ctgctttcct aagtattctc ttgctgagaa caggtgtctt acatatgctt ttgtatattc ctgatctaca tatgatgcac caagttcgtc taacggagaa aaatcattct tccatttttc atttttgata ttgataacac caccccatgt gaacaacata ccatttctgg cattccgggt aggcattaca caatccatca tatcaatacc attacctata gattccagaa tattccatgg tgttccaacc cccatcagat aacgaggctt atctttagga agaatatctg ttacttcatc tgtaattctg tacatttcct cttccggttc accaacagaa agacctccaa tagcatttcc ttcagctcct gcctcagcaa taacttcagc ggaagctttt ctgagatccg aatatacaga tccctgaacg atcggaaaca atctttgttt gtgtccgtaa tattcaggat tattttccgt ccattcaata catctcttca gccatctgtg ggtaagttcc atagaaactt ttgcctgatt gtattcacat ggatatggag tacattcgtc aaaagccata aagatatctg caccaatttt acgctgaatt tccatagaaa cttcaggtga aataaaatga taacttccgt cgatatgaga tttgaatttc acaccttctt cagtcatttt ccggctggaa gccaatgaga aaacctgaaa accaccacta tccgtaagga taggaccgtt ccagttcata aatttatgta atccgcctgc attctgcata acatccatac ctggtctcag gtacaggtgg tatgtatttc ctaagattat ctgagcctta atatcatctt tcaattctct ctgatgaaca gtttttactg ttgcnacggt acccaccggc ataaaaatag gtgtctgtat tttaccatga tctgttgtaa taacaccagc tctggcttta ccttttgctg aaaattgttc tacttcaaaa aacttctcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHME KFFEVEQFSA KGKARAGVIT TDHGKIQTPI FMPVGTVATV KTVHQRELKD DIKAQIILGN TYHLYLRPGM DVMQNAGGLH KFMNWNGPIL TDSGGFQVFS LASSRKMTEE GVKFKSHIDG SYHFISPEVS MEIQRKIGAD IFMAFDECTP YPCEYNQAKV SMELTHRWLK RCIEWTENNP EYYGHKQRLF PIVQGSVYSD LRKASAEVIA EAGAEGNAIG GLSVGEPEEE MYRITDEVTD ILPKDKPRYL MGVGTPWNIL ESIGNGIDMM DCVMPTRNAR NGMLFTWGGV INIKNEKWKN DFSPLDELGA SYVDQEYTKA YVRHLFSARE YLGKQIASIH NLAFYLDLVK VAREHILAGD FYEWKDSIIP QLKSRM
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAGA AGTTTTTTGA AGTAGAACAA TTTTCAGCAA AAGGTAAAGC CAGAGCTGGT GTTATTACAA CAGATCATGG TAAAATACAG ACACCTATTT TTATGCCGGT GGGTACCGTA GCAACAGTAA AAACTGTTCA TCAGAGAGAA TTGAAAGATG ATATTAAGGC TCAGATAATC TTAGGAAATA CATACCACCT GTACCTGAGA CCAGGTATGG ATGTTATGCA GAATGCAGGC GGATTACATA AATTTATGAA CTGGAACGGT CCTATCCTTA CGGATAGTGG TGGTTTTCAG GTTTTCTCAT TGGCTTCCAG CCGGAAAATG ACTGAAGAAG GTGTGAAATT CAAATCTCAT ATCGACGGAA GTTATCATTT TATTTCACCT GAAGTTTCTA TGGAAATTCA GCGTAAAATT GGTGCAGATA TCTTTATGGC TTTTGACGAA TGTACTCCAT ATCCATGTGA ATACAATCAG GCAAAAGTTT CTATGGAACT TACCCACAGA TGGCTGAAGA GATGTATTGA ATGGACGGAA AATAATCCTG AATATTACGG ACACAAACAA AGATTGTTTC CGATCGTTCA GGGATCTGTA TATTCGGATC TCAGAAAAGC TTCCGCTGAA GTTATTGCTG AGGCAGGAGC TGAAGGAAAT GCTATTGGAG GTCTTTCTGT TGGTGAACCG GAAGAGGAAA TGTACAGAAT TACAGATGAA GTAACAGATA TTCTTCCTAA AGATAAGCCT CGTTATCTGA TGGGGGTTGG AACACCATGG AATATTCTGG AATCTATAGG TAATGGTATT GATATGATGG ATTGTGTAAT GCCTACCCGG AATGCCAGAA ATGGTATGTT GTTCACATGG GGTGGTGTTA TCAATATCAA AAATGAAAAA TGGAAGAATG ATTTTTCTCC GTTAGACGAA CTTGGTGCAT CATATGTAGA TCAGGAATAT ACAAAAGCAT ATGTAAGACA CCTGTTCTCA GCAAGAGAAT ACTTAGGAAA GCAGATTGCT TCTATTCATA ATCTTGCTTT TTATTTAGAT TTGGTAAAAG TCGCAAGAGA ACATATCTTA GCAGGAGATT TCTATGAATG GAAAGATAGC ATTATTCCAC AACTAAAAAG CAGAATGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT