ElanA.00177.a

Chaperone protein DnaK

CENTER ID: ElanA.00177.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00177.a.B1.GE40577 Full length(ElanA.00177.a) 1 628
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00177.a.B1.PW38275 Full length(ElanA.00177.a) 1 628

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.1718
UniProt: A0A077EAC8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKIIGIDLG TTNSCVAVME GKDPVVIPNA EGKRTTPSIV AFTEDGERKV GDPAKRQAVT NPVNTVYSIK RFIGTHFKDD GSEIARVPYK VVSGPNDTVK VKIDDREYTP QEISAMILQK MKKTAEDYLG QEVTRAVITV PAYFNDAQRQ ATKEAGEIAG LKVERIINEP TAAALAYGLD KSHKDQKIAV YDLGGGTFDI SILDLGDGVF EVLSTNGDTH LGGDDFDDVI INWMADEFKA EEGVDLKADA IALQRLKEAA EKAKIELSSS SQTEINLPYI TATATGPKHL VKTLTRAKFE QLSADLVRRS MEPCKKALQD AGLSTSDIDE VILVGGSTRI PIIQEEVEKF FGKKPSKGVN PDEVVAVGAA IQGGVLTGDV KDVLLLDVTP LSLGIETMGS VFTKLIEANT TIPTKKSEVF STASDNQPAV TIRVGQGERP MFNDNKEIGR FELTDIPPAP RGVPQIEVTF DIDANGILSV SAKDKGTGKE QSIKIQASSG LSDEEIERMK KEAQENASAD AKKKEEVEVF NKADGLIFQT EKQLKEFGDK LSADKKSAIE AAAAELKTAF DAKDTEAVKA KTEALDAAWM AASEEMYAQQ QANPQAGAQQ NAGGGAEDVQ DADFEEVK
NT Sequence
ATGAGCAAAA TAATCGGAAT TGACTTAGGA ACAACCAACT CTTGTGTTGC CGTAATGGAG GGGAAAGACC CTGTTGTTAT TCCTAATGCG GAAGGTAAAA GAACAACACC TTCTATCGTA GCATTCACTG AAGATGGTGA AAGAAAAGTA GGTGATCCTG CAAAAAGACA AGCTGTAACC AATCCGGTAA ATACAGTTTA TTCTATCAAA AGATTTATCG GAACTCACTT TAAAGATGAT GGTTCTGAAA TAGCTAGAGT TCCTTACAAA GTTGTAAGCG GACCAAACGA TACTGTAAAA GTAAAAATTG ACGACAGAGA ATATACACCA CAGGAGATTT CAGCAATGAT CCTTCAGAAA ATGAAGAAAA CAGCTGAAGA TTATCTTGGC CAGGAAGTAA CAAGAGCTGT AATTACTGTT CCTGCTTACT TTAACGATGC ACAAAGACAG GCTACAAAAG AAGCCGGAGA AATTGCAGGT CTTAAAGTGG AAAGAATTAT CAACGAGCCT ACAGCTGCTG CTTTGGCTTA CGGATTAGAT AAGAGCCACA AAGATCAGAA GATTGCTGTA TATGACCTTG GAGGTGGTAC TTTCGATATT TCTATCCTTG ACTTAGGTGA TGGTGTATTC GAAGTTTTAT CTACAAACGG TGATACGCAC CTTGGTGGTG ATGACTTCGA TGATGTAATC ATTAACTGGA TGGCAGATGA ATTCAAAGCT GAAGAAGGTG TAGATCTTAA AGCGGATGCT ATCGCTCTTC AACGTTTAAA AGAAGCTGCT GAAAAAGCTA AAATCGAGTT ATCTTCTTCT TCTCAGACTG AAATTAACTT ACCATATATT ACTGCAACGG CTACAGGTCC TAAGCACTTG GTTAAAACTT TAACCAGAGC TAAGTTCGAG CAATTATCTG CTGATCTTGT AAGACGTTCT ATGGAGCCTT GTAAAAAAGC TCTTCAGGAT GCAGGACTTT CAACTTCTGA TATCGACGAA GTAATCTTAG TAGGTGGTTC TACACGTATT CCTATTATCC AAGAAGAAGT AGAAAAATTC TTTGGTAAAA AACCTTCTAA AGGTGTTAAC CCGGATGAAG TAGTAGCTGT TGGTGCTGCA ATTCAGGGTG GTGTATTAAC AGGTGATGTA AAAGATGTAT TACTTCTTGA CGTAACGCCA CTTTCTTTAG GTATTGAAAC TATGGGTTCT GTATTCACTA AACTAATTGA GGCGAATACA ACTATCCCAA CTAAAAAATC TGAAGTATTC TCTACTGCAT CCGACAATCA GCCGGCAGTA ACTATCAGAG TAGGACAAGG GGAGAGACCA ATGTTCAACG ATAACAAAGA AATCGGTAGA TTCGAATTAA CAGATATTCC ACCAGCACCA AGAGGAGTTC CTCAGATTGA AGTAACTTTC GATATCGATG CTAATGGTAT CTTAAGTGTA TCTGCTAAAG ATAAAGGAAC TGGTAAAGAG CAGTCTATCA AAATTCAGGC TTCTTCTGGC CTTTCTGACG AAGAAATCGA AAGAATGAAA AAAGAAGCTC AGGAAAATGC TTCTGCAGAT GCTAAGAAAA AAGAAGAAGT TGAAGTATTC AACAAAGCTG ATGGATTAAT CTTCCAGACT GAAAAACAAT TGAAAGAGTT TGGAGATAAG CTTTCTGCTG ATAAAAAATC TGCAATTGAA GCTGCAGCAG CCGAACTTAA AACTGCTTTT GATGCTAAAG ATACTGAAGC TGTTAAAGCT AAAACTGAAG CTTTAGATGC AGCATGGATG GCAGCATCTG AAGAAATGTA TGCACAACAA CAAGCTAACC CGCAGGCTGG TGCTCAGCAA AATGCAGGTG GTGGTGCAGA AGATGTACAG GACGCTGACT TCGAAGAAGT AAAA
Details for ElanA.00177.a.B1.GE40577
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 66
PERCENT COVERAGE: 56
Validated AA Sequence
MAHHHHHHHM SKIIGIDXXT TNSCVAVMEG KDPVVIPNAE GKRTTPSIVX FTEDGERKVX DPAKRQAVTN PVNTVYSIKR FIGTHFKDDG SEIARVPYKV VSGPNDTVKV KIDDREYTPQ EISAMILXXM KKTAEXYLGQ EVTRAVITVP AYFNDAQRQA TKEAGEIAGL KVERIINEPT AAALAYGLDK SHKDQKIAVY DLGGGTXXIS ILDXXDGVFE VLSTNGDTHL GGDDFDDVII NWMAXEXXXX XXVXLXXXXX XXQRXXXXXX XAXXXXXSXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXRRSRXXXX
Validated NT Sequence
tcctgtacat cttctgcacc accacctgca ttttgctgag caccagnctg cgggttannt tgttgttgtg catacatttc ttcagatgct gccatccatg ctgcatctaa agcttcagtt ttagctttaa cagcttcagt atctttagca tcaaaagcag ttttaagttc ggctgctgca gcttcaattg cagatttttt atcagcagaa agcttatctc caaactcttt caattgtttt tcagtctgga agattaatcc atcnnntttg ttgaatactt caacttcttc ttttttcttn nnatctgcag aagcattttc ctgagcttct tttttcattc tttnnntttc ttcnnnagaa aggccagaag annnctgaat tttgatagac tgctctttac cagttccttt atctttagca gatacactta agataccatt nnnatcgata tcgaaagtta cttcaatctg aggaactcct cttggtgctg gtggaatatc tgttaattcg aatctaccga tttctttgtt atcgttgaac attgnnntct ccccttgtcc tactctgata gttactgccg gctgattgtc ggatgcagtn nagaatactt cnnntttttt nnntgggata gttgtattcn nnnnaattnn nnnnnngaat acnnnaccca tnnnttcaat acctaaagaa agnnncgtta nnncannnng taatacatct tttacnnnnn ntgttaatac accaccctga attncnnnnn nnncnnnnnn nnnnnnnnnc nnnnnnnaca cctnnnnnnn nnnnnttacn nnnnnnnntt tctacttctt cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgnnnnnnn nnnnnnnnnn nnnnnnttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntnnnncn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnannn nnnnnnnntt ncnnnnnnnn nnnnngaagn nnnnnnnnnn nnagcnnnnn nannnnnnnn nnnnaaacgt tgnngnnnnn nnnnnnnnnn nnnaagannn acannnnnnt cannnnnnna ttcatntgcc atccagttaa tgattacatc atcgaagtca tcaccaccaa ggtgcgtatc accgtttgta gataaaactt cgaatacacc atcnnnnnng tcaaggatag aaatannnaa agtaccacct ccaaggtcat atacagcaat cttctgatct ttgtggctct tatctaatcc gtaagccaaa gcagcagctg taggctcgtt gataattctt tccactttaa gacctgcaat ttctccggct tcttttgtag cctgtctttg tgcatcgtta aagtaagcag gaacagtaat tacagctctt gttacttcct ggccaagata ancttcagct gttttcttca tttnnngaag gatcattgcn gaaatctcct gtggtgtata ttctctgtcg tcaattttta cttttacagt atcgtttggt ccgcttacaa ctttgtaagg aactctagct atttcagaac catcatcttt aaagtgagtt ccgataaatc ttttgataga ataaactgta tttaccggat tggttacagc ttgtcttttt gcaggatcan nnacttttct ttcaccatct tcagtgaatn nnacgataga aggtgttgtt cttttacctt ccgcattagg aataacaaca gggtctttcc cctccattac ggcaacacaa gagttggttg ttnnnaagtc aattccgatt attttgctca tatggtggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMS KIIGIDLGTT NSCVAVMEGK DPVVIPNAEG KRTTPSIVAF TEDGERKVGD PAKRQAVTNP VNTVYSIKRF IGTHFKDDGS EIARVPYKVV SGPNDTVKVK IDDREYTPQE ISAMILQKMK KTAEDYLGQE VTRAVITVPA YFNDAQRQAT KEAGEIAGLK VERIINEPTA AALAYGLDKS HKDQKIAVYD LGGGTFDISI LDLGDGVFEV LSTNGDTHLG GDDFDDVIIN WMADEFKAEE GVDLKADAIA LQRLKEAAEK AKIELSSSSQ TEINLPYITA TATGPKHLVK TLTRAKFEQL SADLVRRSME PCKKALQDAG LSTSDIDEVI LVGGSTRIPI IQEEVEKFFG KKPSKGVNPD EVVAVGAAIQ GGVLTGDVKD VLLLDVTPLS LGIETMGSVF TKLIEANTTI PTKKSEVFST ASDNQPAVTI RVGQGERPMF NDNKEIGRFE LTDIPPAPRG VPQIEVTFDI DANGILSVSA KDKGTGKEQS IKIQASSGLS DEEIERMKKE AQENASADAK KKEEVEVFNK ADGLIFQTEK QLKEFGDKLS ADKKSAIEAA AAELKTAFDA KDTEAVKAKT EALDAAWMAA SEEMYAQQQA NPQAGAQQNA GGGAEDVQDA DFEEVK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca aaataatcgg aattgactta ggaacaacca actcttgtgt tgccgtaatg gaggggaaag accctgttgt tattcctaat gcggaaggta aaagaacaac accttctatc gtagcattca ctgaagatgg tgaaagaaaa gtaggtgatc ctgcaaaaag acaagctgta accaatccgg taaatacagt ttattctatc aaaagattta tcggaactca ctttaaagat gatggttctg aaatagctag agttccttac aaagttgtaa gcggaccaaa cgatactgta aaagtaaaaa ttgacgacag agaatataca ccacaggaga tttcagcaat gatccttcag aaaatgaaga aaacagctga agattatctt ggccaggaag taacaagagc tgtaattact gttcctgctt actttaacga tgcacaaaga caggctacaa aagaagccgg agaaattgca ggtcttaaag tggaaagaat tatcaacgag cctacagctg ctgctttggc ttacggatta gataagagcc acaaagatca gaagattgct gtatatgacc ttggaggtgg tactttcgat atttctatcc ttgacttagg tgatggtgta ttcgaagttt tatctacaaa cggtgatacg caccttggtg gtgatgactt cgatgatgta atcattaact ggatggcaga tgaattcaaa gctgaagaag gtgtagatct taaagcggat gctatcgctc ttcaacgttt aaaagaagct gctgaaaaag ctaaaatcga gttatcttct tcttctcaga ctgaaattaa cttaccatat attactgcaa cggctacagg tcctaagcac ttggttaaaa ctttaaccag agctaagttc gagcaattat ctgctgatct tgtaagacgt tctatggagc cttgtaaaaa agctcttcag gatgcaggac tttcaacttc tgatatcgac gaagtaatct tagtaggtgg ttctacacgt attcctatta tccaagaaga agtagaaaaa ttctttggta aaaaaccttc taaaggtgtt aacccggatg aagtagtagc tgttggtgct gcaattcagg gtggtgtatt aacaggtgat gtaaaagatg tattacttct tgacgtaacg ccactttctt taggtattga aactatgggt tctgtattca ctaaactaat tgaggcgaat acaactatcc caactaaaaa atctgaagta ttctctactg catccgacaa tcagccggca gtaactatca gagtaggaca aggggagaga ccaatgttca acgataacaa agaaatcggt agattcgaat taacagatat tccaccagca ccaagaggag ttcctcagat tgaagtaact ttcgatatcg atgctaatgg tatcttaagt gtatctgcta aagataaagg aactggtaaa gagcagtcta tcaaaattca ggcttcttct ggcctttctg acgaagaaat cgaaagaatg aaaaaagaag ctcaggaaaa tgcttctgca gatgctaaga aaaaagaaga agttgaagta ttcaacaaag ctgatggatt aatcttccag actgaaaaac aattgaaaga gtttggagat aagctttctg ctgataaaaa atctgcaatt gaagctgcag cagccgaact taaaactgct tttgatgcta aagatactga agctgttaaa gctaaaactg aagctttaga tgcagcatgg atggcagcat ctgaagaaat gtatgcacaa caacaagcta acccgcaggc tggtgctcag caaaatgcag gtggtggtgc agaagatgta caggacgctg acttcgaaga agtaaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for ElanA.00177.a.B1.PW38275
PURIFICATION DATe: 5/16/2017
CONCENTRATION: 21mg/ml
OBSERVED MW: 75kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 66
PERCENT COVERAGE: 56
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHHM SKIIGIDXXT TNSCVAVMEG KDPVVIPNAE GKRTTPSIVX FTEDGERKVX DPAKRQAVTN PVNTVYSIKR FIGTHFKDDG SEIARVPYKV VSGPNDTVKV KIDDREYTPQ EISAMILXXM KKTAEXYLGQ EVTRAVITVP AYFNDAQRQA TKEAGEIAGL KVERIINEPT AAALAYGLDK SHKDQKIAVY DLGGGTXXIS ILDXXDGVFE VLSTNGDTHL GGDDFDDVII NWMAXEXXXX XXVXLXXXXX XXQRXXXXXX XAXXXXXSXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXRRSRXXXX
Validated NT Sequence
tcctgtacat cttctgcacc accacctgca ttttgctgag caccagnctg cgggttannt tgttgttgtg catacatttc ttcagatgct gccatccatg ctgcatctaa agcttcagtt ttagctttaa cagcttcagt atctttagca tcaaaagcag ttttaagttc ggctgctgca gcttcaattg cagatttttt atcagcagaa agcttatctc caaactcttt caattgtttt tcagtctgga agattaatcc atcnnntttg ttgaatactt caacttcttc ttttttcttn nnatctgcag aagcattttc ctgagcttct tttttcattc tttnnntttc ttcnnnagaa aggccagaag annnctgaat tttgatagac tgctctttac cagttccttt atctttagca gatacactta agataccatt nnnatcgata tcgaaagtta cttcaatctg aggaactcct cttggtgctg gtggaatatc tgttaattcg aatctaccga tttctttgtt atcgttgaac attgnnntct ccccttgtcc tactctgata gttactgccg gctgattgtc ggatgcagtn nagaatactt cnnntttttt nnntgggata gttgtattcn nnnnaattnn nnnnnngaat acnnnaccca tnnnttcaat acctaaagaa agnnncgtta nnncannnng taatacatct tttacnnnnn ntgttaatac accaccctga attncnnnnn nnncnnnnnn nnnnnnnnnc nnnnnnnaca cctnnnnnnn nnnnnttacn nnnnnnnntt tctacttctt cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgnnnnnnn nnnnnnnnnn nnnnnnttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntnnnncn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnannn nnnnnnnntt ncnnnnnnnn nnnnngaagn nnnnnnnnnn nnagcnnnnn nannnnnnnn nnnnaaacgt tgnngnnnnn nnnnnnnnnn nnnaagannn acannnnnnt cannnnnnna ttcatntgcc atccagttaa tgattacatc atcgaagtca tcaccaccaa ggtgcgtatc accgtttgta gataaaactt cgaatacacc atcnnnnnng tcaaggatag aaatannnaa agtaccacct ccaaggtcat atacagcaat cttctgatct ttgtggctct tatctaatcc gtaagccaaa gcagcagctg taggctcgtt gataattctt tccactttaa gacctgcaat ttctccggct tcttttgtag cctgtctttg tgcatcgtta aagtaagcag gaacagtaat tacagctctt gttacttcct ggccaagata ancttcagct gttttcttca tttnnngaag gatcattgcn gaaatctcct gtggtgtata ttctctgtcg tcaattttta cttttacagt atcgtttggt ccgcttacaa ctttgtaagg aactctagct atttcagaac catcatcttt aaagtgagtt ccgataaatc ttttgataga ataaactgta tttaccggat tggttacagc ttgtcttttt gcaggatcan nnacttttct ttcaccatct tcagtgaatn nnacgataga aggtgttgtt cttttacctt ccgcattagg aataacaaca gggtctttcc cctccattac ggcaacacaa gagttggttg ttnnnaagtc aattccgatt attttgctca tatggtggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMS KIIGIDLGTT NSCVAVMEGK DPVVIPNAEG KRTTPSIVAF TEDGERKVGD PAKRQAVTNP VNTVYSIKRF IGTHFKDDGS EIARVPYKVV SGPNDTVKVK IDDREYTPQE ISAMILQKMK KTAEDYLGQE VTRAVITVPA YFNDAQRQAT KEAGEIAGLK VERIINEPTA AALAYGLDKS HKDQKIAVYD LGGGTFDISI LDLGDGVFEV LSTNGDTHLG GDDFDDVIIN WMADEFKAEE GVDLKADAIA LQRLKEAAEK AKIELSSSSQ TEINLPYITA TATGPKHLVK TLTRAKFEQL SADLVRRSME PCKKALQDAG LSTSDIDEVI LVGGSTRIPI IQEEVEKFFG KKPSKGVNPD EVVAVGAAIQ GGVLTGDVKD VLLLDVTPLS LGIETMGSVF TKLIEANTTI PTKKSEVFST ASDNQPAVTI RVGQGERPMF NDNKEIGRFE LTDIPPAPRG VPQIEVTFDI DANGILSVSA KDKGTGKEQS IKIQASSGLS DEEIERMKKE AQENASADAK KKEEVEVFNK ADGLIFQTEK QLKEFGDKLS ADKKSAIEAA AAELKTAFDA KDTEAVKAKT EALDAAWMAA SEEMYAQQQA NPQAGAQQNA GGGAEDVQDA DFEEVK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA AAATAATCGG AATTGACTTA GGAACAACCA ACTCTTGTGT TGCCGTAATG GAGGGGAAAG ACCCTGTTGT TATTCCTAAT GCGGAAGGTA AAAGAACAAC ACCTTCTATC GTAGCATTCA CTGAAGATGG TGAAAGAAAA GTAGGTGATC CTGCAAAAAG ACAAGCTGTA ACCAATCCGG TAAATACAGT TTATTCTATC AAAAGATTTA TCGGAACTCA CTTTAAAGAT GATGGTTCTG AAATAGCTAG AGTTCCTTAC AAAGTTGTAA GCGGACCAAA CGATACTGTA AAAGTAAAAA TTGACGACAG AGAATATACA CCACAGGAGA TTTCAGCAAT GATCCTTCAG AAAATGAAGA AAACAGCTGA AGATTATCTT GGCCAGGAAG TAACAAGAGC TGTAATTACT GTTCCTGCTT ACTTTAACGA TGCACAAAGA CAGGCTACAA AAGAAGCCGG AGAAATTGCA GGTCTTAAAG TGGAAAGAAT TATCAACGAG CCTACAGCTG CTGCTTTGGC TTACGGATTA GATAAGAGCC ACAAAGATCA GAAGATTGCT GTATATGACC TTGGAGGTGG TACTTTCGAT ATTTCTATCC TTGACTTAGG TGATGGTGTA TTCGAAGTTT TATCTACAAA CGGTGATACG CACCTTGGTG GTGATGACTT CGATGATGTA ATCATTAACT GGATGGCAGA TGAATTCAAA GCTGAAGAAG GTGTAGATCT TAAAGCGGAT GCTATCGCTC TTCAACGTTT AAAAGAAGCT GCTGAAAAAG CTAAAATCGA GTTATCTTCT TCTTCTCAGA CTGAAATTAA CTTACCATAT ATTACTGCAA CGGCTACAGG TCCTAAGCAC TTGGTTAAAA CTTTAACCAG AGCTAAGTTC GAGCAATTAT CTGCTGATCT TGTAAGACGT TCTATGGAGC CTTGTAAAAA AGCTCTTCAG GATGCAGGAC TTTCAACTTC TGATATCGAC GAAGTAATCT TAGTAGGTGG TTCTACACGT ATTCCTATTA TCCAAGAAGA AGTAGAAAAA TTCTTTGGTA AAAAACCTTC TAAAGGTGTT AACCCGGATG AAGTAGTAGC TGTTGGTGCT GCAATTCAGG GTGGTGTATT AACAGGTGAT GTAAAAGATG TATTACTTCT TGACGTAACG CCACTTTCTT TAGGTATTGA AACTATGGGT TCTGTATTCA CTAAACTAAT TGAGGCGAAT ACAACTATCC CAACTAAAAA ATCTGAAGTA TTCTCTACTG CATCCGACAA TCAGCCGGCA GTAACTATCA GAGTAGGACA AGGGGAGAGA CCAATGTTCA ACGATAACAA AGAAATCGGT AGATTCGAAT TAACAGATAT TCCACCAGCA CCAAGAGGAG TTCCTCAGAT TGAAGTAACT TTCGATATCG ATGCTAATGG TATCTTAAGT GTATCTGCTA AAGATAAAGG AACTGGTAAA GAGCAGTCTA TCAAAATTCA GGCTTCTTCT GGCCTTTCTG ACGAAGAAAT CGAAAGAATG AAAAAAGAAG CTCAGGAAAA TGCTTCTGCA GATGCTAAGA AAAAAGAAGA AGTTGAAGTA TTCAACAAAG CTGATGGATT AATCTTCCAG ACTGAAAAAC AATTGAAAGA GTTTGGAGAT AAGCTTTCTG CTGATAAAAA ATCTGCAATT GAAGCTGCAG CAGCCGAACT TAAAACTGCT TTTGATGCTA AAGATACTGA AGCTGTTAAA GCTAAAACTG AAGCTTTAGA TGCAGCATGG ATGGCAGCAT CTGAAGAAAT GTATGCACAA CAACAAGCTA ACCCGCAGGC TGGTGCTCAG CAAAATGCAG GTGGTGGTGC AGAAGATGTA CAGGACGCTG ACTTCGAAGA AGTAAAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT