ElanA.00163.a

Phenylalanine--tRNA ligase alpha subunit

CENTER ID: ElanA.00163.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00163.a.B1.GE41083 Full length(ElanA.00163.a) 1 336
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.2280
UniProt: A0A077EBA2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLEKIEELLG QVQQFHTKNK EEIEKFRIAF IGKKGSVTEL FEKFKDVPNE QKKEFGQKIN TLKQAVNAKI EDLKAGISEE TITEKIDLTK PGFPEELGSR HPINLVKNRI IEIFKSIGFA VADGPEIEDD WHNFTALNLP EYHPARDMQD TFFIEKDPDI LLRTHTSSVQ IRYMENNEPP MRILSPGRVF RNEAISSRSH CIFHQIEGLY IDKNVSFADL KQTIQYFTTE LFGKSQIRMR PSYFPFTEPS AEVDVYWGLN SETDYRITKG TGWLEIMGCG MVDPAVLKNV NINSDEYSGF AFGMGIERIV MLLYQMSDIR MFFENDVRML EQFKHL
NT Sequence
ATGCTAGAGA AAATAGAAGA ATTACTGGGT CAGGTACAAC AATTTCATAC CAAAAATAAA GAAGAAATCG AGAAGTTCCG TATTGCCTTT ATTGGTAAGA AAGGAAGTGT GACAGAGCTT TTTGAGAAGT TTAAAGATGT TCCGAATGAA CAGAAAAAAG AATTCGGGCA GAAAATTAAT ACCCTGAAGC AGGCTGTAAA TGCTAAGATT GAAGATCTGA AAGCAGGGAT TTCTGAAGAA ACGATTACCG AGAAAATAGA CCTTACAAAA CCTGGATTTC CTGAAGAGTT AGGAAGCAGA CATCCTATTA ATCTGGTTAA GAACAGAATT ATTGAAATCT TTAAATCTAT TGGTTTTGCT GTAGCAGACG GACCAGAGAT TGAAGACGAC TGGCATAATT TTACAGCATT GAACTTACCG GAATATCACC CGGCAAGAGA TATGCAGGAT ACTTTCTTTA TTGAGAAAGA TCCGGATATT CTTTTACGAA CGCACACTTC TTCTGTACAG ATCAGATATA TGGAAAACAA CGAACCTCCG ATGAGAATTT TATCACCGGG AAGAGTTTTC CGTAACGAGG CTATTTCATC CCGTTCACAC TGTATATTCC ATCAGATAGA AGGTTTGTAT ATAGACAAGA ATGTATCATT TGCAGATCTT AAACAAACAA TTCAGTATTT TACGACAGAA TTATTTGGTA AATCTCAAAT CAGAATGCGT CCTTCTTATT TTCCGTTTAC TGAGCCAAGT GCTGAGGTAG ATGTATACTG GGGACTAAAC TCTGAAACTG ATTACAGAAT TACTAAAGGT ACGGGTTGGT TAGAGATTAT GGGTTGCGGA ATGGTAGATC CTGCTGTTCT GAAAAATGTA AACATTAATT CTGATGAGTA CAGTGGTTTT GCTTTTGGTA TGGGAATCGA GCGTATCGTA ATGTTGCTTT ACCAAATGTC TGATATCCGT ATGTTCTTTG AAAATGATGT ACGTATGTTG GAGCAATTCA AACATTTG
Details for ElanA.00163.a.B1.GE41083
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 40kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML EKIEELLGQV QQFHTKNKEE IEKFRIAFIG KKGSVTELFE KFKDVPNEQK KEFGQKINTL KQAVNAKIED LKAGISEETI TEKIDLTKPG FPEELGSRHP INLVKNRIIE IFKSIGFAVA DGPEIEDDWH NFTALNLPEY HPARDMQDTF FIEKDPDILL RTHTSSVQIR YMENNEPPMR ILSPGRVFRN EAISSRSHCI FHQIEGLYID KNVSFADLKQ TIQYFTTELF GKSQIRMRPS YFPFTEPSAE VDVYWGLNSE TDYRITKGTG WLEIMGCGMV DPAVLKNVNI NSDEYSGFAF GMGIERIVML LYQMSDIRMF FENDVR
Validated NT Sequence
tacgtacatc attttcaaag aacatacgga tatcagacat ttggtaaagc aacattacga tacgctcgat tcccatacca aaagcaaaac cactgtactc atcagaatta atgtttacat ttttcagaac agcaggatct accattccgc aacccataat ctctaaccaa cccgtacctt tagtaattct gtaatcagtt tcagagttta gtccccagta tacatctacc tcagcacttg gctcagtaaa cggaaaataa gaaggacgca ttctgatttg agatttacca aataattctg tcgtaaaata ctgaattgtt tgtttaagat ctgcaaatga tacattcttg tctatataca aaccttctat ctgatggaat atacagtgtg aacgggatga aatagcctcg ttacggaaaa ctcttcccgg tgataaaatt ctcatcggag gttcgttgtt ttccatatat ctgatctgta cagaagaagt gtgcgttcgt aaaagaatat ccggatcttt ctcaataaag aaagtatcct gcatatctct tgccgggtga tattccggta agttcaatgc tgtaaaatta tgccagtcgt cttcaatctc tggtccgtct gctacagcaa aaccaataga tttaaagatt tcaataattc tgttcttaac cagattaata ggatgtctgc ttcctaactc ttcaggaaat ccaggttttg taaggtctat tttctcggta atcgtttctt cagaaatccc tgctttcaga tcttcaatct tagcatttac agcctgcttc agggtattaa ttttctgccc gaattctttt ttctgttcat tcggaacatc tttaaacttc tcaaaaagct ctgtcacact tcctttctta ccaataaagg caatacggaa cttctcgatt tcttctttat ttttggtatg aaattgttgt acctgaccca gtaattcttc tattttctct agcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHML EKIEELLGQV QQFHTKNKEE IEKFRIAFIG KKGSVTELFE KFKDVPNEQK KEFGQKINTL KQAVNAKIED LKAGISEETI TEKIDLTKPG FPEELGSRHP INLVKNRIIE IFKSIGFAVA DGPEIEDDWH NFTALNLPEY HPARDMQDTF FIEKDPDILL RTHTSSVQIR YMENNEPPMR ILSPGRVFRN EAISSRSHCI FHQIEGLYID KNVSFADLKQ TIQYFTTELF GKSQIRMRPS YFPFTEPSAE VDVYWGLNSE TDYRITKGTG WLEIMGCGMV DPAVLKNVNI NSDEYSGFAF GMGIERIVML LYQMSDIRMF FENDVRMLEQ FKHL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctag agaaaataga agaattactg ggtcaggtac aacaatttca taccaaaaat aaagaagaaa tcgagaagtt ccgtattgcc tttattggta agaaaggaag tgtgacagag ctttttgaga agtttaaaga tgttccgaat gaacagaaaa aagaattcgg gcagaaaatt aataccctga agcaggctgt aaatgctaag attgaagatc tgaaagcagg gatttctgaa gaaacgatta ccgagaaaat agaccttaca aaacctggat ttcctgaaga gttaggaagc agacatccta ttaatctggt taagaacaga attattgaaa tctttaaatc tattggtttt gctgtagcag acggaccaga gattgaagac gactggcata attttacagc attgaactta ccggaatatc acccggcaag agatatgcag gatactttct ttattgagaa agatccggat attcttttac gaacgcacac ttcttctgta cagatcagat atatggaaaa caacgaacct ccgatgagaa ttttatcacc gggaagagtt ttccgtaacg aggctatttc atcccgttca cactgtatat tccatcagat agaaggtttg tatatagaca agaatgtatc atttgcagat cttaaacaaa caattcagta ttttacgaca gaattatttg gtaaatctca aatcagaatg cgtccttctt attttccgtt tactgagcca agtgctgagg tagatgtata ctggggacta aactctgaaa ctgattacag aattactaaa ggtacgggtt ggttagagat tatgggttgc ggaatggtag atcctgctgt tctgaaaaat gtaaacatta attctgatga gtacagtggt tttgcttttg gtatgggaat cgagcgtatc gtaatgttgc tttaccaaat gtctgatatc cgtatgttct ttgaaaatga tgtacgtatg ttggagcaat tcaaacattt gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat