ElanA.00156.a

Threonine--tRNA ligase

CENTER ID: ElanA.00156.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00156.a.B1.GE41082 Full length(ElanA.00156.a) 1 643
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00156.a.B1.PW38252 Full length(ElanA.00156.a) 1 643

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.2716
UniProt: A0A077EFC2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIKITLPDGS IKEFEAAVTP LQVAQSISEG LARNTISALV NGTQTEVNTP ITEDATVQLF TWNDDMGKKA FWHSSAHLLA QAILDFYPEA KLTIGPAIEN GFYYDVDFGD QVFSEKDFEK VEKKMLENAK KNSTFNLYPV SKAEALETYK DNPYKTELIE NLTDGEITFC SHDNFFDLCR GGHIPATGIV KAAKILNAAG AYWRGDEKNK QLTRVYGITF PKQKDLTEYL ERLEEAKRRD HRKLGKELGI FAFSEKVGAG LPLWLPKGAA LRKKLENFLS AAQKKQGYEF VISPHIGHKD LYVTSGHWEK YGADSFQPIK TPHEGEEFLL KPMNCPHHCE IYKAQQWSYK DLPKRYAEFG TVYRYEQSGE LHGLTRVRGF TQDDAHLFCT PDQLLEEFEK VIDLVLYVFS SLGFADFTAQ VSLRDKENRE KYIGSEENWE KAEHAIINAA NKKGLKTVIE YGEAAFYGPK LDFMVKDALG RSWQLGTIQV DYNLPERFDL WYTGSDNDKH RPVMIHRAPF GSMERFIAIL IENTAGDFPL WLSPEQFIIL PISEKYVDYA KKVSQLLEIH DICGLIDERN EKTGKKIRDA ELKKIPYMLV VGENEAQNGS VSVRRRGEGD LGAMSIDDFV QHVKKEIANH LNK
NT Sequence
ATGATAAAGA TCACACTTCC GGACGGAAGC ATTAAAGAAT TCGAGGCAGC GGTAACTCCG CTACAGGTGG CGCAATCCAT TAGTGAAGGA TTGGCACGTA ACACTATTTC GGCATTGGTA AACGGGACAC AGACAGAAGT GAACACGCCA ATAACGGAAG ATGCCACGGT TCAGCTTTTT ACCTGGAACG ATGATATGGG TAAAAAAGCT TTCTGGCATT CCTCTGCTCA CCTTTTAGCT CAGGCTATTC TGGATTTTTA TCCGGAAGCT AAACTAACTA TAGGACCTGC AATTGAAAAC GGGTTCTACT ACGATGTAGA TTTTGGTGAC CAGGTTTTTT CCGAAAAAGA TTTCGAAAAA GTTGAAAAGA AAATGCTGGA GAATGCTAAA AAGAACTCAA CTTTTAATCT ATATCCGGTT TCCAAGGCTG AAGCATTAGA AACTTATAAA GATAACCCTT ACAAAACAGA GCTAATCGAA AATTTAACTG ATGGCGAAAT CACTTTCTGC TCTCACGATA ATTTCTTCGA CTTATGTCGT GGTGGCCACA TCCCGGCAAC CGGAATTGTA AAGGCAGCCA AAATTCTAAA CGCTGCTGGT GCATACTGGA GAGGTGATGA AAAAAACAAA CAGCTAACTC GTGTATACGG AATTACTTTC CCTAAACAGA AAGATTTAAC AGAATATCTG GAGCGTCTGG AGGAAGCGAA GCGTCGTGAC CACCGTAAAT TAGGTAAGGA ATTAGGAATT TTCGCTTTCT CAGAAAAAGT GGGTGCTGGT TTACCATTAT GGTTGCCAAA AGGGGCGGCT CTTAGAAAGA AACTAGAAAA TTTCCTTTCT GCAGCACAGA AAAAACAAGG ATATGAATTC GTTATCTCTC CACATATCGG TCACAAAGAT TTATATGTAA CTTCCGGTCA CTGGGAGAAA TACGGAGCAG ATAGTTTCCA ACCAATTAAA ACTCCACACG AAGGAGAAGA GTTTTTATTA AAACCAATGA ACTGTCCTCA CCATTGTGAA ATCTACAAAG CACAGCAATG GTCTTATAAA GACCTTCCAA AACGCTATGC TGAGTTTGGT ACAGTTTACA GATACGAGCA AAGTGGAGAA CTTCACGGCT TAACCAGAGT TAGAGGATTT ACTCAGGATG ATGCTCACCT TTTCTGTACT CCGGATCAGT TATTGGAAGA ATTCGAAAAA GTAATTGATT TGGTGCTTTA TGTCTTCTCT TCTTTAGGAT TTGCTGATTT TACAGCTCAG GTTTCTTTAA GAGACAAAGA AAACAGAGAA AAATATATCG GTTCTGAAGA AAATTGGGAA AAGGCAGAAC ACGCTATTAT CAATGCTGCT AATAAGAAAG GATTAAAAAC TGTTATTGAA TACGGTGAAG CTGCTTTCTA CGGACCAAAA TTAGACTTTA TGGTGAAAGA TGCCCTAGGC AGAAGCTGGC AGCTTGGAAC CATTCAGGTA GATTATAACT TACCAGAGCG TTTTGATCTT TGGTATACAG GTTCTGATAA TGACAAGCAC AGACCGGTAA TGATTCACCG TGCTCCGTTT GGATCTATGG AAAGATTCAT TGCAATCCTT ATAGAGAATA CAGCCGGAGA TTTCCCGTTA TGGCTTAGCC CTGAGCAATT TATCATTTTA CCAATCAGCG AAAAATATGT AGATTATGCA AAAAAAGTTT CGCAATTGTT AGAAATTCAC GATATTTGCG GTCTGATTGA CGAAAGGAAT GAGAAAACGG GTAAGAAAAT CCGTGATGCA GAACTTAAGA AAATCCCATA TATGCTTGTT GTAGGCGAAA ATGAGGCTCA AAACGGAAGC GTTTCTGTAA GAAGAAGAGG CGAAGGAGAT TTGGGAGCCA TGTCGATAGA CGATTTCGTA CAACATGTAA AAAAAGAAAT AGCTAACCAT TTAAATAAA
Details for ElanA.00156.a.B1.GE41082
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 74kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMI KITLPDGSIK EFEAAVTPLQ VAQSISEGLA RNTISALVNG TQTEVNTPIT EDATVQLFTW NDDMGKKAFW HSSAHLLAQA ILDFYPEAKL TIGPAIENGF YYDVDFGDQV FSEKDFEKVE KKMLENAKKN STFNLYPVSK AEALETYKDN PYKTELIENL TDGEITFCSH DNFFDLCRGG HIPATGIVKA AKILNAAGAY WRGDEKNKQL TRVYGITFPK QKDLTEYLER LEEAKRRDHR KLGKELGIFA FSEKVGAGLP LWLPKGAALR KKLENFLSAA QKKQGYEFVI SPHIGHKDLY VTSGHWEKYG ADSFQPIKTP HEGEEFLLKP MNCPHHCEIY KAQQWSYKDL PKRYAEFGTV YRYEQSGELH GLTRVRGFTQ DDAHLFCTPD QLLEEFEKVI DLVLYVFSSL GFADFTAQVS LRDKENREKY IGSEENWEKA EHAIINAANK KGLKTVIEYG EAAFYGPKLD FMVKDALGRS WQLGTIQVDY NLPERFDLWY TGSDNDKHRP VMIHRAPFGS MERFIAILIE NTAGDFPLWL SPEQFIILPI SEKYVDYAKK VSQLLEIHDI CGLIDERNEK TGKKIRDAEL KKIPYMLVVG ENEAQNGSVS VRRRGEGDLG AMSIDDFVQH VKKEIANHLN K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgataa agatcacact tccggacgga agcattaaag aattcgaggc agcggtaact ccgctacagg tggcgcaatc cattagtgaa ggattggcac gtaacactat ttcggcattg gtaaacggga cacagacaga agtgaacacg ccaataacgg aagatgccac ggttcagctt tttacctgga acgatgatat gggtaaaaaa gctttctggc attcctctgc tcacctttta gctcaggcta ttctggattt ttatccggaa gctaaactaa ctataggacc tgcaattgaa aacgggttct actacgatgt agattttggt gaccaggttt tttccgaaaa agatttcgaa aaagttgaaa agaaaatgct ggagaatgct aaaaagaact caacttttaa tctatatccg gtttccaagg ctgaagcatt agaaacttat aaagataacc cttacaaaac agagctaatc gaaaatttaa ctgatggcga aatcactttc tgctctcacg ataatttctt cgacttatgt cgtggtggcc acatcccggc aaccggaatt gtaaaggcag ccaaaattct aaacgctgct ggtgcatact ggagaggtga tgaaaaaaac aaacagctaa ctcgtgtata cggaattact ttccctaaac agaaagattt aacagaatat ctggagcgtc tggaggaagc gaagcgtcgt gaccaccgta aattaggtaa ggaattagga attttcgctt tctcagaaaa agtgggtgct ggtttaccat tatggttgcc aaaaggggcg gctcttagaa agaaactaga aaatttcctt tctgcagcac agaaaaaaca aggatatgaa ttcgttatct ctccacatat cggtcacaaa gatttatatg taacttccgg tcactgggag aaatacggag cagatagttt ccaaccaatt aaaactccac acgaaggaga agagttttta ttaaaaccaa tgaactgtcc tcaccattgt gaaatctaca aagcacagca atggtcttat aaagaccttc caaaacgcta tgctgagttt ggtacagttt acagatacga gcaaagtgga gaacttcacg gcttaaccag agttagagga tttactcagg atgatgctca ccttttctgt actccggatc agttattgga agaattcgaa aaagtaattg atttggtgct ttatgtcttc tcttctttag gatttgctga ttttacagct caggtttctt taagagacaa agaaaacaga gaaaaatata tcggttctga agaaaattgg gaaaaggcag aacacgctat tatcaatgct gctaataaga aaggattaaa aactgttatt gaatacggtg aagctgcttt ctacggacca aaattagact ttatggtgaa agatgcccta ggcagaagct ggcagcttgg aaccattcag gtagattata acttaccaga gcgttttgat ctttggtata caggttctga taatgacaag cacagaccgg taatgattca ccgtgctccg tttggatcta tggaaagatt cattgcaatc cttatagaga atacagccgg agatttcccg ttatggctta gccctgagca atttatcatt ttaccaatca gcgaaaaata tgtagattat gcaaaaaaag tttcgcaatt gttagaaatt cacgatattt gcggtctgat tgacgaaagg aatgagaaaa cgggtaagaa aatccgtgat gcagaactta agaaaatccc atatatgctt gttgtaggcg aaaatgaggc tcaaaacgga agcgtttctg taagaagaag aggcgaagga gatttgggag ccatgtcgat agacgatttc gtacaacatg taaaaaaaga aatagctaac catttaaata aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for ElanA.00156.a.B1.PW38252
PURIFICATION DATe: 4/11/2017
CONCENTRATION: 30mg/ml
OBSERVED MW: 73kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMI KITLPDGSIK EFEAAVTPLQ VAQSISEGLA RNTISALVNG TQTEVNTPIT EDATVQLFTW NDDMGKKAFW HSSAHLLAQA ILDFYPEAKL TIGPAIENGF YYDVDFGDQV FSEKDFEKVE KKMLENAKKN STFNLYPVSK AEALETYKDN PYKTELIENL TDGEITFCSH DNFFDLCRGG HIPATGIVKA AKILNAAGAY WRGDEKNKQL TRVYGITFPK QKDLTEYLER LEEAKRRDHR KLGKELGIFA FSEKVGAGLP LWLPKGAALR KKLENFLSAA QKKQGYEFVI SPHIGHKDLY VTSGHWEKYG ADSFQPIKTP HEGEEFLLKP MNCPHHCEIY KAQQWSYKDL PKRYAEFGTV YRYEQSGELH GLTRVRGFTQ DDAHLFCTPD QLLEEFEKVI DLVLYVFSSL GFADFTAQVS LRDKENREKY IGSEENWEKA EHAIINAANK KGLKTVIEYG EAAFYGPKLD FMVKDALGRS WQLGTIQVDY NLPERFDLWY TGSDNDKHRP VMIHRAPFGS MERFIAILIE NTAGDFPLWL SPEQFIILPI SEKYVDYAKK VSQLLEIHDI CGLIDERNEK TGKKIRDAEL KKIPYMLVVG ENEAQNGSVS VRRRGEGDLG AMSIDDFVQH VKKEIANHLN K
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATAA AGATCACACT TCCGGACGGA AGCATTAAAG AATTCGAGGC AGCGGTAACT CCGCTACAGG TGGCGCAATC CATTAGTGAA GGATTGGCAC GTAACACTAT TTCGGCATTG GTAAACGGGA CACAGACAGA AGTGAACACG CCAATAACGG AAGATGCCAC GGTTCAGCTT TTTACCTGGA ACGATGATAT GGGTAAAAAA GCTTTCTGGC ATTCCTCTGC TCACCTTTTA GCTCAGGCTA TTCTGGATTT TTATCCGGAA GCTAAACTAA CTATAGGACC TGCAATTGAA AACGGGTTCT ACTACGATGT AGATTTTGGT GACCAGGTTT TTTCCGAAAA AGATTTCGAA AAAGTTGAAA AGAAAATGCT GGAGAATGCT AAAAAGAACT CAACTTTTAA TCTATATCCG GTTTCCAAGG CTGAAGCATT AGAAACTTAT AAAGATAACC CTTACAAAAC AGAGCTAATC GAAAATTTAA CTGATGGCGA AATCACTTTC TGCTCTCACG ATAATTTCTT CGACTTATGT CGTGGTGGCC ACATCCCGGC AACCGGAATT GTAAAGGCAG CCAAAATTCT AAACGCTGCT GGTGCATACT GGAGAGGTGA TGAAAAAAAC AAACAGCTAA CTCGTGTATA CGGAATTACT TTCCCTAAAC AGAAAGATTT AACAGAATAT CTGGAGCGTC TGGAGGAAGC GAAGCGTCGT GACCACCGTA AATTAGGTAA GGAATTAGGA ATTTTCGCTT TCTCAGAAAA AGTGGGTGCT GGTTTACCAT TATGGTTGCC AAAAGGGGCG GCTCTTAGAA AGAAACTAGA AAATTTCCTT TCTGCAGCAC AGAAAAAACA AGGATATGAA TTCGTTATCT CTCCACATAT CGGTCACAAA GATTTATATG TAACTTCCGG TCACTGGGAG AAATACGGAG CAGATAGTTT CCAACCAATT AAAACTCCAC ACGAAGGAGA AGAGTTTTTA TTAAAACCAA TGAACTGTCC TCACCATTGT GAAATCTACA AAGCACAGCA ATGGTCTTAT AAAGACCTTC CAAAACGCTA TGCTGAGTTT GGTACAGTTT ACAGATACGA GCAAAGTGGA GAACTTCACG GCTTAACCAG AGTTAGAGGA TTTACTCAGG ATGATGCTCA CCTTTTCTGT ACTCCGGATC AGTTATTGGA AGAATTCGAA AAAGTAATTG ATTTGGTGCT TTATGTCTTC TCTTCTTTAG GATTTGCTGA TTTTACAGCT CAGGTTTCTT TAAGAGACAA AGAAAACAGA GAAAAATATA TCGGTTCTGA AGAAAATTGG GAAAAGGCAG AACACGCTAT TATCAATGCT GCTAATAAGA AAGGATTAAA AACTGTTATT GAATACGGTG AAGCTGCTTT CTACGGACCA AAATTAGACT TTATGGTGAA AGATGCCCTA GGCAGAAGCT GGCAGCTTGG AACCATTCAG GTAGATTATA ACTTACCAGA GCGTTTTGAT CTTTGGTATA CAGGTTCTGA TAATGACAAG CACAGACCGG TAATGATTCA CCGTGCTCCG TTTGGATCTA TGGAAAGATT CATTGCAATC CTTATAGAGA ATACAGCCGG AGATTTCCCG TTATGGCTTA GCCCTGAGCA ATTTATCATT TTACCAATCA GCGAAAAATA TGTAGATTAT GCAAAAAAAG TTTCGCAATT GTTAGAAATT CACGATATTT GCGGTCTGAT TGACGAAAGG AATGAGAAAA CGGGTAAGAA AATCCGTGAT GCAGAACTTA AGAAAATCCC ATATATGCTT GTTGTAGGCG AAAATGAGGC TCAAAACGGA AGCGTTTCTG TAAGAAGAAG AGGCGAAGGA GATTTGGGAG CCATGTCGAT AGACGATTTC GTACAACATG TAAAAAAAGA AATAGCTAAC CATTTAAATA AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT