SSGCID: Target Page

ElanA.00145.a

Aspartyl-tRNA synthetase

CENTER ID:	ElanA.00145.a
ORGANISM:	Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.00145.a.B1.GE41081	Full length(ElanA.00145.a)		1	584

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.00145.a.B1.PW38254	Full length(ElanA.00145.a)		1	584

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1338011.4.peg.1538
UniProt:	A0A077EBQ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFRTHTNGEL SLKNLNEEVT LSGWVQTIRD KGFMIWIDLR DRYGITQLVF DQDRSSATLL EEAKKLGREF VIQVNGKVIE RASKNPKIPT GEIEILVEKL TILNNSELPP FTIEDETDGG EELRMKYRYL DIRRNPVKEK LIFRHKMAQK VRNYLSDQGF IEVETPVLIK STPEGARDFV VPSRMNPGQF YALPQSPQTF KQLLMVGGMD KYFQIVKCFR DEDLRADRQP EFTQIDCEMA FVEQEDVMNI FEGLTQNLLK DIAGQEFGKF PRMTFADAMK KYGNDKPDIR FGMEFHELNN LVKGKDFKIF DEAELVVGIN VEGCAEYTRK QIDELTDWVK RPQIGATGMV WIKYQADGIV TSSVNKFYNE EDLKKIAEEF GAKPGDLMLV LSGNENKVRA QLSALRMELG NRLGLRKGNE FAPLWVIDFP LLEWDEDTQR YHAMHHPFTS PKPEDIHLLE NEAGKARANA YDLVINGNEI GGGSIRIFDK DLQAQMFSLL GFTPEEAEAQ FGFLMNAFKY GAPPHGGLAF GFDRLVAVLD GNEVIRDYIA FPKNNSGRDV MIDAPASIAN EQLDELALTI NINE

NT Sequence

ATGTTCCGAA CGCACACCAA CGGAGAATTA TCTCTGAAGA ATCTAAACGA AGAAGTAACA CTTTCAGGAT GGGTACAAAC TATCCGTGAT AAAGGTTTTA TGATTTGGAT AGATTTACGA GATCGTTACG GAATAACACA GCTGGTTTTC GATCAGGATC GCTCTTCTGC TACATTACTG GAAGAAGCAA AAAAACTAGG CCGTGAATTT GTAATTCAGG TTAACGGAAA AGTAATTGAA AGAGCTAGTA AAAATCCTAA GATACCGACC GGAGAAATTG AAATTCTGGT TGAAAAGTTA ACTATTCTGA ACAATTCCGA GCTACCTCCT TTCACTATTG AGGATGAAAC CGATGGCGGT GAAGAACTTA GAATGAAGTA CCGTTACCTG GATATCAGAA GAAATCCGGT AAAAGAAAAG TTAATCTTCC GTCACAAAAT GGCTCAGAAA GTAAGAAATT ATCTTTCTGA CCAAGGATTT ATTGAAGTAG AAACACCGGT TCTTATCAAA TCTACGCCTG AAGGTGCAAG AGACTTTGTT GTACCAAGCA GAATGAATCC GGGCCAGTTT TATGCATTGC CACAATCTCC GCAGACTTTC AAACAATTAT TGATGGTTGG CGGAATGGAT AAATACTTCC AGATTGTAAA ATGTTTCCGT GACGAGGACC TAAGAGCCGA CAGACAGCCG GAGTTCACTC AGATAGACTG TGAAATGGCT TTCGTAGAGC AGGAAGACGT TATGAATATT TTCGAAGGCC TTACTCAGAA TTTATTAAAG GATATAGCAG GTCAGGAATT TGGAAAATTC CCGAGGATGA CTTTCGCAGA CGCTATGAAA AAATATGGTA ATGACAAACC GGATATTCGT TTCGGAATGG AATTCCATGA ACTAAATAAC CTTGTAAAAG GTAAAGATTT CAAAATCTTT GACGAAGCCG AACTGGTTGT TGGTATCAAT GTAGAGGGTT GTGCAGAGTA CACGAGAAAA CAAATCGACG AATTAACCGA CTGGGTAAAA CGTCCACAGA TAGGCGCTAC AGGTATGGTA TGGATAAAAT ATCAGGCCGA TGGTATTGTA ACTTCTTCTG TTAACAAATT CTATAACGAA GAAGATTTAA AGAAAATTGC AGAAGAATTT GGCGCCAAGC CAGGTGACTT AATGCTTGTA CTTTCCGGAA ACGAAAATAA AGTAAGAGCA CAGTTATCTG CATTGAGAAT GGAATTGGGT AACCGCCTTG GATTAAGAAA AGGAAATGAA TTTGCTCCAC TTTGGGTTAT TGATTTCCCG CTTTTAGAAT GGGATGAAGA TACACAGAGA TACCACGCAA TGCATCACCC TTTTACTTCT CCTAAGCCGG AAGATATACA CTTACTGGAA AATGAAGCAG GAAAAGCAAG AGCTAATGCT TACGACTTAG TTATCAACGG TAACGAAATC GGCGGTGGAT CTATCAGAAT CTTTGATAAA GATCTTCAGG CTCAGATGTT CAGCCTTCTT GGGTTTACAC CGGAAGAAGC AGAAGCACAA TTTGGTTTCT TGATGAATGC CTTCAAATAC GGAGCACCTC CACACGGTGG TTTAGCTTTC GGATTTGACA GACTAGTAGC TGTATTAGAC GGAAATGAAG TCATCAGAGA TTACATCGCC TTCCCTAAAA ACAATAGTGG TCGAGATGTA ATGATCGACG CTCCGGCATC CATTGCCAAT GAGCAACTCG ATGAATTAGC ATTAACAATT AACATTAACG AA

Details for ElanA.00145.a.B1.GE41081

HARVESTED ON:	2/8/2017
SEQUENCED ON:	2/13/2017
EXPECTED MW:	68kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMF RTHTNGELSL KNLNEEVTLS GWVQTIRDKG FMIWIDLRDR YGITQLVFDQ DRSSATLLEE AKKLGREFVI QVNGKVIERA SKNPKIPTGE IEILVEKLTI LNNSELPPFT IEDETDGGEE LRMKYRYLDI RRNPVKEKLI FRHKMAQKVR NYLSDQGFIE VETPVLIKST PEGARDFVVP SRMNPGQFYA LPQSPQTFKQ LLMVGGMDKY FQIVKCFRDE DLRADRQPEF TQIDCEMAFV EQEDVMNIFE XLTQNLLKDI AGQEFGKFPR MTFADAMKKY GNDKPDIRFG MEFHELNNLV KGKDFKIXDE AELVVGINVE GCAEYTRKQI DELTDWVKRP QIGATGMVWI KYQADGIVTS SVNKFYNEED LKKIAEEFGA KPGDLMLVLS GNENKVRAQL SALRMELGNR LGLRKGNEFA PLWVIDFPLL EWDEDTQRYH AMHHPFTSPK PEDIHLLENE AGKARANAYD LVINGNEIGG GSIRIFDKDL QAQMFSLLGF TPEEAEAQFG FLMNAFKYGA PPHGGLAFGF DRLVAVLDGN EVIRDYIAFP KNNSGRDVMI DAPASIANEQ LDELALTINI NE

Validated NT Sequence

atggctcacc accaccacca ccatatgttc cgaacgcaca ccaacggaga attatctctg aagaatctaa acgaagaagt aacactttca ggatgggtac aaactatccg tgataaaggt tttatgattt ggatagattt acgagatcgt tacggaataa cacagctggt tttcgatcag gatcgctctt ctgctacatt actggaagaa gcaaaaaaac taggccgtga atttgtaatt caggttaacg gaaaagtaat tgaaagagct agtaaaaatc ctaagatacc gaccggagaa attgaaattc tggttgaaaa gttaactatt ctgaacaatt ccgagctacc tcctttcact attgaggatg aaaccgatgg cggtgaagaa cttagaatga agtaccgtta cctggatatc agaagaaatc cggtaaaaga aaagttaatc ttccgtcaca aaatggctca gaaagtaaga aattatcttt ctgaccaagg atttattgaa gtagaaacac cggttcttat caaatctacg cctgaaggtg caagagactt tgttgtacca agcagaatga atccgggcca gttttatgca ttgccacaat ctccgcagac tttcaaacaa ttattgatgg ttggcggaat ggataaatac ttccagattg taaaatgttt ccgtgacgag gacctaagag ccgacagaca gccggagttc actcagatag actgtgaaat ggctttcgta gagcaggaag acgttatgaa tattttcgaa ngccttactc agaatttatt aaaggatata gcaggtcagg aatttggaaa attcccgagg atgactttcg cagacgctat gaaaaaatat ggtaatgaca aaccggatat tcgtttcgga atggaa-ttc catgaactaa a-taaccttg taaaaggtaa agatttcaaa atcttngacg aagccgaact ggttgttggt atcaatgtag agggttgtgc agagtacacg agaaaacaaa tcgacgaatt aaccgactgg gtaaaacgtc cacagatagg cgctacaggt atggtatgga taaaatatca ggccgatggt attgtaactt cttctgttaa caaattctat aacgaagaag atttaaagaa aattgcagaa gaatttggcg ccaagccagg tgacttaatg cttgtacttt ccggaaacga aaataaagta agagcacagt tatctgcatt gagaatggaa ttgggtaacc gccttggatt aagaaaagga aatgaatttg ctccactttg ggttattgat ttcccgcttt tagaatggga tgaagataca cagagatacc acgcaatgca tcaccctttt acttctccta agccggaaga tatacactta ctggaaaatg aagcaggaaa agcaagagct aatgcttacg acttagttat caacggtaac gaaatcggcg gtggatctat cagaatcttt gataaagatc ttcaggctca gatgttcagc cttcttgggt ttacaccgga agaagcagaa gcacaatttg gtttcttgat gaatgccttc aaatacggag cacctccaca cggtggttta gctttcggat ttgacagact agtagctgta ttagacggaa atgaagtcat cagagattac atcgccttcc ctaaaaacaa tagtggtcga gatgtaatga tcgacgctcc ggcatccatt gccaatgagc aactcgatga attagcatta acaattaaca ttaacgaa

Expected Protein Sequence

MAHHHHHHMF RTHTNGELSL KNLNEEVTLS GWVQTIRDKG FMIWIDLRDR YGITQLVFDQ DRSSATLLEE AKKLGREFVI QVNGKVIERA SKNPKIPTGE IEILVEKLTI LNNSELPPFT IEDETDGGEE LRMKYRYLDI RRNPVKEKLI FRHKMAQKVR NYLSDQGFIE VETPVLIKST PEGARDFVVP SRMNPGQFYA LPQSPQTFKQ LLMVGGMDKY FQIVKCFRDE DLRADRQPEF TQIDCEMAFV EQEDVMNIFE GLTQNLLKDI AGQEFGKFPR MTFADAMKKY GNDKPDIRFG MEFHELNNLV KGKDFKIFDE AELVVGINVE GCAEYTRKQI DELTDWVKRP QIGATGMVWI KYQADGIVTS SVNKFYNEED LKKIAEEFGA KPGDLMLVLS GNENKVRAQL SALRMELGNR LGLRKGNEFA PLWVIDFPLL EWDEDTQRYH AMHHPFTSPK PEDIHLLENE AGKARANAYD LVINGNEIGG GSIRIFDKDL QAQMFSLLGF TPEEAEAQFG FLMNAFKYGA PPHGGLAFGF DRLVAVLDGN EVIRDYIAFP KNNSGRDVMI DAPASIANEQ LDELALTINI NE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcc gaacgcacac caacggagaa ttatctctga agaatctaaa cgaagaagta acactttcag gatgggtaca aactatccgt gataaaggtt ttatgatttg gatagattta cgagatcgtt acggaataac acagctggtt ttcgatcagg atcgctcttc tgctacatta ctggaagaag caaaaaaact aggccgtgaa tttgtaattc aggttaacgg aaaagtaatt gaaagagcta gtaaaaatcc taagataccg accggagaaa ttgaaattct ggttgaaaag ttaactattc tgaacaattc cgagctacct cctttcacta ttgaggatga aaccgatggc ggtgaagaac ttagaatgaa gtaccgttac ctggatatca gaagaaatcc ggtaaaagaa aagttaatct tccgtcacaa aatggctcag aaagtaagaa attatctttc tgaccaagga tttattgaag tagaaacacc ggttcttatc aaatctacgc ctgaaggtgc aagagacttt gttgtaccaa gcagaatgaa tccgggccag ttttatgcat tgccacaatc tccgcagact ttcaaacaat tattgatggt tggcggaatg gataaatact tccagattgt aaaatgtttc cgtgacgagg acctaagagc cgacagacag ccggagttca ctcagataga ctgtgaaatg gctttcgtag agcaggaaga cgttatgaat attttcgaag gccttactca gaatttatta aaggatatag caggtcagga atttggaaaa ttcccgagga tgactttcgc agacgctatg aaaaaatatg gtaatgacaa accggatatt cgtttcggaa tggaattcca tgaactaaat aaccttgtaa aaggtaaaga tttcaaaatc tttgacgaag ccgaactggt tgttggtatc aatgtagagg gttgtgcaga gtacacgaga aaacaaatcg acgaattaac cgactgggta aaacgtccac agataggcgc tacaggtatg gtatggataa aatatcaggc cgatggtatt gtaacttctt ctgttaacaa attctataac gaagaagatt taaagaaaat tgcagaagaa tttggcgcca agccaggtga cttaatgctt gtactttccg gaaacgaaaa taaagtaaga gcacagttat ctgcattgag aatggaattg ggtaaccgcc ttggattaag aaaaggaaat gaatttgctc cactttgggt tattgatttc ccgcttttag aatgggatga agatacacag agataccacg caatgcatca cccttttact tctcctaagc cggaagatat acacttactg gaaaatgaag caggaaaagc aagagctaat gcttacgact tagttatcaa cggtaacgaa atcggcggtg gatctatcag aatctttgat aaagatcttc aggctcagat gttcagcctt cttgggttta caccggaaga agcagaagca caatttggtt tcttgatgaa tgccttcaaa tacggagcac ctccacacgg tggtttagct ttcggatttg acagactagt agctgtatta gacggaaatg aagtcatcag agattacatc gccttcccta aaaacaatag tggtcgagat gtaatgatcg acgctccggc atccattgcc aatgagcaac tcgatgaatt agcattaaca attaacatta acgaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for ElanA.00145.a.B1.PW38254

PURIFICATION DATe:	4/11/2017
CONCENTRATION:	25mg/ml
OBSERVED MW:	78kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMF RTHTNGELSL KNLNEEVTLS GWVQTIRDKG FMIWIDLRDR YGITQLVFDQ DRSSATLLEE AKKLGREFVI QVNGKVIERA SKNPKIPTGE IEILVEKLTI LNNSELPPFT IEDETDGGEE LRMKYRYLDI RRNPVKEKLI FRHKMAQKVR NYLSDQGFIE VETPVLIKST PEGARDFVVP SRMNPGQFYA LPQSPQTFKQ LLMVGGMDKY FQIVKCFRDE DLRADRQPEF TQIDCEMAFV EQEDVMNIFE GLTQNLLKDI AGQEFGKFPR MTFADAMKKY GNDKPDIRFG MEFHELNNLV KGKDFKIFDE AELVVGINVE GCAEYTRKQI DELTDWVKRP QIGATGMVWI KYQADGIVTS SVNKFYNEED LKKIAEEFGA KPGDLMLVLS GNENKVRAQL SALRMELGNR LGLRKGNEFA PLWVIDFPLL EWDEDTQRYH AMHHPFTSPK PEDIHLLENE AGKARANAYD LVINGNEIGG GSIRIFDKDL QAQMFSLLGF TPEEAEAQFG FLMNAFKYGA PPHGGLAFGF DRLVAVLDGN EVIRDYIAFP KNNSGRDVMI DAPASIANEQ LDELALTINI NE

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTCC GAACGCACAC CAACGGAGAA TTATCTCTGA AGAATCTAAA CGAAGAAGTA ACACTTTCAG GATGGGTACA AACTATCCGT GATAAAGGTT TTATGATTTG GATAGATTTA CGAGATCGTT ACGGAATAAC ACAGCTGGTT TTCGATCAGG ATCGCTCTTC TGCTACATTA CTGGAAGAAG CAAAAAAACT AGGCCGTGAA TTTGTAATTC AGGTTAACGG AAAAGTAATT GAAAGAGCTA GTAAAAATCC TAAGATACCG ACCGGAGAAA TTGAAATTCT GGTTGAAAAG TTAACTATTC TGAACAATTC CGAGCTACCT CCTTTCACTA TTGAGGATGA AACCGATGGC GGTGAAGAAC TTAGAATGAA GTACCGTTAC CTGGATATCA GAAGAAATCC GGTAAAAGAA AAGTTAATCT TCCGTCACAA AATGGCTCAG AAAGTAAGAA ATTATCTTTC TGACCAAGGA TTTATTGAAG TAGAAACACC GGTTCTTATC AAATCTACGC CTGAAGGTGC AAGAGACTTT GTTGTACCAA GCAGAATGAA TCCGGGCCAG TTTTATGCAT TGCCACAATC TCCGCAGACT TTCAAACAAT TATTGATGGT TGGCGGAATG GATAAATACT TCCAGATTGT AAAATGTTTC CGTGACGAGG ACCTAAGAGC CGACAGACAG CCGGAGTTCA CTCAGATAGA CTGTGAAATG GCTTTCGTAG AGCAGGAAGA CGTTATGAAT ATTTTCGAAG GCCTTACTCA GAATTTATTA AAGGATATAG CAGGTCAGGA ATTTGGAAAA TTCCCGAGGA TGACTTTCGC AGACGCTATG AAAAAATATG GTAATGACAA ACCGGATATT CGTTTCGGAA TGGAATTCCA TGAACTAAAT AACCTTGTAA AAGGTAAAGA TTTCAAAATC TTTGACGAAG CCGAACTGGT TGTTGGTATC AATGTAGAGG GTTGTGCAGA GTACACGAGA AAACAAATCG ACGAATTAAC CGACTGGGTA AAACGTCCAC AGATAGGCGC TACAGGTATG GTATGGATAA AATATCAGGC CGATGGTATT GTAACTTCTT CTGTTAACAA ATTCTATAAC GAAGAAGATT TAAAGAAAAT TGCAGAAGAA TTTGGCGCCA AGCCAGGTGA CTTAATGCTT GTACTTTCCG GAAACGAAAA TAAAGTAAGA GCACAGTTAT CTGCATTGAG AATGGAATTG GGTAACCGCC TTGGATTAAG AAAAGGAAAT GAATTTGCTC CACTTTGGGT TATTGATTTC CCGCTTTTAG AATGGGATGA AGATACACAG AGATACCACG CAATGCATCA CCCTTTTACT TCTCCTAAGC CGGAAGATAT ACACTTACTG GAAAATGAAG CAGGAAAAGC AAGAGCTAAT GCTTACGACT TAGTTATCAA CGGTAACGAA ATCGGCGGTG GATCTATCAG AATCTTTGAT AAAGATCTTC AGGCTCAGAT GTTCAGCCTT CTTGGGTTTA CACCGGAAGA AGCAGAAGCA CAATTTGGTT TCTTGATGAA TGCCTTCAAA TACGGAGCAC CTCCACACGG TGGTTTAGCT TTCGGATTTG ACAGACTAGT AGCTGTATTA GACGGAAATG AAGTCATCAG AGATTACATC GCCTTCCCTA AAAACAATAG TGGTCGAGAT GTAATGATCG ACGCTCCGGC ATCCATTGCC AATGAGCAAC TCGATGAATT AGCATTAACA ATTAACATTA ACGAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT