ElanA.00133.a

Cysteine--tRNA ligase

CENTER ID: ElanA.00133.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00133.a.B1.GE41080 Full length(ElanA.00133.a) 1 487
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00133.a.B1.PW38251 Full length(ElanA.00133.a) 1 487

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.1509
UniProt: A0A077E9B7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLKIYNSLT GEKEDFKPIL DGNVGMYVCG PTVYSNVHLG NVRTFLSFDF IYRSLQFLGY KVRYVRNITD AGHLTDDGNI DNDRFIKQSR LEKLEPMEIV QKYTVDFHEV LKKFNLLPPT IEPTATGHII EQIELTKKLI EKGFAYESNG SVYFDVLEYN ARGLNYGELS RRNIEELFAN TRDLDGQGEK KNPQDFALWK KASPQHIMRW ISPWGEGFPG WHLECTAMST KYLGDKFDIH GGGMDLKFPH HECEIAQGKA CNGTEPVNYW MHANMLTMNG QRMSKSTGNY ILPMELITGN NNFFEKPFHP SVLRFCFLQA HYRSVLDISN EAMLASEKGF SRLMDAVKIL DEIQPSDVST VNVQEWLDKA YSALVDDFNS PILISHLFEA VKWIFILKDG KETITANDLI LLKEKLNAFI FDVLGLQTIT EEAGSDKLDE TLQLLIELRN QARKTKNWEL SDQIRDRLLE KGIELKDGRE GTSYTIN
NT Sequence
ATGCAACTTA AAATATATAA CTCGCTTACA GGAGAAAAAG AAGACTTCAA ACCTATATTA GACGGAAACG TGGGAATGTA TGTCTGCGGA CCTACAGTAT ATAGTAATGT TCATTTAGGG AATGTAAGAA CTTTTCTTTC CTTTGACTTT ATATATAGAT CCCTGCAATT TCTGGGATAT AAAGTAAGGT ATGTAAGAAA TATTACTGAT GCGGGACATT TAACAGATGA CGGAAACATA GATAATGATC GTTTCATTAA ACAATCCAGG CTGGAAAAGC TGGAGCCAAT GGAAATTGTA CAGAAGTATA CAGTCGATTT TCATGAGGTA CTAAAGAAAT TTAACCTGCT TCCGCCTACA ATAGAGCCAA CTGCGACAGG ACATATCATC GAGCAGATTG AACTGACAAA AAAATTAATT GAAAAGGGAT TTGCTTACGA AAGCAATGGG TCGGTTTATT TCGATGTTTT GGAATATAAT GCAAGAGGAC TTAACTACGG AGAACTAAGC CGCAGAAATA TAGAAGAACT TTTTGCCAAT ACCCGAGACC TGGATGGCCA GGGTGAGAAA AAAAATCCAC AGGATTTTGC ACTTTGGAAG AAAGCATCCC CACAACATAT TATGCGTTGG ATTTCTCCTT GGGGGGAAGG TTTTCCGGGG TGGCACCTGG AGTGTACAGC AATGAGTACT AAATATCTTG GAGATAAATT CGATATTCAT GGTGGTGGGA TGGATCTTAA ATTTCCACAC CATGAATGTG AGATTGCCCA GGGAAAAGCT TGCAATGGTA CAGAACCGGT AAACTACTGG ATGCATGCCA ATATGCTGAC AATGAATGGG CAGCGAATGA GTAAATCTAC AGGTAATTAT ATTCTGCCAA TGGAGTTAAT TACCGGGAAT AATAACTTCT TCGAGAAACC ATTCCATCCA AGCGTGTTGC GTTTCTGTTT CCTTCAGGCA CACTACAGAA GTGTACTGGA TATTTCCAAT GAGGCAATGT TGGCAAGTGA AAAGGGCTTT AGCCGCCTGA TGGATGCTGT AAAAATATTG GATGAAATCC AACCTTCTGA TGTTTCTACG GTTAATGTTC AGGAATGGTT GGATAAAGCT TATTCCGCGC TTGTTGACGA TTTCAATAGT CCAATACTTA TATCGCATCT TTTTGAAGCG GTAAAATGGA TTTTCATTCT TAAAGATGGG AAAGAAACAA TTACAGCAAA TGACCTTATA TTGCTGAAAG AAAAGCTGAA TGCTTTTATA TTTGATGTGC TGGGATTACA AACCATAACA GAAGAAGCAG GAAGTGATAA ACTGGATGAA ACATTACAGT TATTGATTGA ACTGAGAAAT CAGGCAAGAA AAACTAAAAA CTGGGAGTTA TCGGATCAGA TAAGGGACCG CCTTCTGGAA AAAGGTATTG AACTAAAAGA CGGAAGAGAG GGGACATCTT ATACGATTAA C
Details for ElanA.00133.a.B1.GE41080
HARVESTED ON: 2/8/2017
SEQUENCED ON: 2/13/2017
EXPECTED MW: 57kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMQ LKIYNSLTGE KEDFKPILDG NVGMYVCGPT VYSNVHLGNV RTFLSFDFIY RSLQFLGYKV RYVRNITDAG HLTDDGNIDN DRFIKQSRLE KLEPMEIVQK YTVDFHEVLK KFNLLPPTIE PTATGHIIEQ IELTKKLIEK GFAYESNGSV YFDVLEYNAR GLNYGELSRR NIEELFANTR DLDGQGEKKN PQDFALWKKA SPQHIMRWIS PWGEGFPGWH LECTAMSTKY LGDKFDIHGG GMDLKFPHHE CEIAQGKACN GTEPVNYWMH ANMLTMNGQR MSKSTGNYIL PMELITGNNN FFEKPFHPSV LRFCFLQAHY RSVLDISNEA MLASEKGFSR LMDAVKILDE IQPSDVSTVN VQEWLDKAYS ALVDDFNSPI LISHLFEAVK WIFILKDGKE TITANDLILL KEKLNAFIFD VLGLQTITEE AGSDKLDETL QLLIELRNQA RKTKNWELSD QIRDRLLEKG IELKDGR
Validated NT Sequence
ctctcttccg tcttttagtt caataccttt ttccagaagg cggtccctta tctgatccga taactcccag tttttagttt ttcttgcctg atttctcagt tcaatcaata actgtaatgt ttcatccagt ttatcacttc ctgcttcttc tgttatggtt tgtaatccca gcacatcaaa tataaaagca ttcagctttt ctttcagcaa tataaggtca tttgctgtaa ttgtttcttt cccatcttta agaatgaaaa tccattttac cgcttcaaaa agatgcgata taagtattgg actattgaaa tcgtcaacaa gcgcggaata agctttatcc aaccattcct gaacattaac cgtagaaaca tcagaaggtt ggatttcatc caatattttt acagcatcca tcaggcggct aaagcccttt tcacttgcca acattgcctc attggaaata tccagtacac ttctgtagtg tgcctgaagg aaacagaaac gcaacacgct tggatggaat ggtttctcga agaagttatt attcccggta attaactcca ttggcagaat ataattacct gtagatttac tcattcgctg cccattcatt gtcagcatat tggcatgcat ccagtagttt accggttctg taccattgca agcttttccc tgggcaatct cacattcatg gtgtggaaat ttaagatcca tcccaccacc atgaatatcg aatttatctc caagatattt agtactcatt gctgtacact ccaggtgcca ccccggaaaa ccttcccccc aaggagaaat ccaacgcata atatgttgtg gggatgcttt cttccaaagt gcaaaatcct gtggattttt tttctcaccc tggccatcca ggtctcgggt attggcaaaa agttcttcta tatttctgcg gcttagttct ccgtagttaa gtcctcttgc attatattcc aaaacatcga aataaaccga cccattgctt tcgtaagcaa atcccttttc aattaatttt tttgtcagtt caatctgctc gatgatatgt cctgtcgcag ttggctctat tgtaggcgga agcaggttaa atttctttag tacctcatga aaatcgactg tatacttctg tacaatttcc attggctcca gcttttccag cctggattgt ttaatgaaac gatcattatc tatgtttccg tcatctgtta aatgtcccgc atcagtaata tttcttacat accttacttt atatcccaga aattgcaggg atctatatat aaagtcaaag gaaagaaaag ttcttacatt ccctaaatga acattactat atactgtagg tccgcagaca tacattccca cgtttccgtc taatataggt ttgaagtctt ctttttctcc tgtaagcgag ttatatattt taagttgcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMQ LKIYNSLTGE KEDFKPILDG NVGMYVCGPT VYSNVHLGNV RTFLSFDFIY RSLQFLGYKV RYVRNITDAG HLTDDGNIDN DRFIKQSRLE KLEPMEIVQK YTVDFHEVLK KFNLLPPTIE PTATGHIIEQ IELTKKLIEK GFAYESNGSV YFDVLEYNAR GLNYGELSRR NIEELFANTR DLDGQGEKKN PQDFALWKKA SPQHIMRWIS PWGEGFPGWH LECTAMSTKY LGDKFDIHGG GMDLKFPHHE CEIAQGKACN GTEPVNYWMH ANMLTMNGQR MSKSTGNYIL PMELITGNNN FFEKPFHPSV LRFCFLQAHY RSVLDISNEA MLASEKGFSR LMDAVKILDE IQPSDVSTVN VQEWLDKAYS ALVDDFNSPI LISHLFEAVK WIFILKDGKE TITANDLILL KEKLNAFIFD VLGLQTITEE AGSDKLDETL QLLIELRNQA RKTKNWELSD QIRDRLLEKG IELKDGREGT SYTIN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaac ttaaaatata taactcgctt acaggagaaa aagaagactt caaacctata ttagacggaa acgtgggaat gtatgtctgc ggacctacag tatatagtaa tgttcattta gggaatgtaa gaacttttct ttcctttgac tttatatata gatccctgca atttctggga tataaagtaa ggtatgtaag aaatattact gatgcgggac atttaacaga tgacggaaac atagataatg atcgtttcat taaacaatcc aggctggaaa agctggagcc aatggaaatt gtacagaagt atacagtcga ttttcatgag gtactaaaga aatttaacct gcttccgcct acaatagagc caactgcgac aggacatatc atcgagcaga ttgaactgac aaaaaaatta attgaaaagg gatttgctta cgaaagcaat gggtcggttt atttcgatgt tttggaatat aatgcaagag gacttaacta cggagaacta agccgcagaa atatagaaga actttttgcc aatacccgag acctggatgg ccagggtgag aaaaaaaatc cacaggattt tgcactttgg aagaaagcat ccccacaaca tattatgcgt tggatttctc cttgggggga aggttttccg gggtggcacc tggagtgtac agcaatgagt actaaatatc ttggagataa attcgatatt catggtggtg ggatggatct taaatttcca caccatgaat gtgagattgc ccagggaaaa gcttgcaatg gtacagaacc ggtaaactac tggatgcatg ccaatatgct gacaatgaat gggcagcgaa tgagtaaatc tacaggtaat tatattctgc caatggagtt aattaccggg aataataact tcttcgagaa accattccat ccaagcgtgt tgcgtttctg tttccttcag gcacactaca gaagtgtact ggatatttcc aatgaggcaa tgttggcaag tgaaaagggc tttagccgcc tgatggatgc tgtaaaaata ttggatgaaa tccaaccttc tgatgtttct acggttaatg ttcaggaatg gttggataaa gcttattccg cgcttgttga cgatttcaat agtccaatac ttatatcgca tctttttgaa gcggtaaaat ggattttcat tcttaaagat gggaaagaaa caattacagc aaatgacctt atattgctga aagaaaagct gaatgctttt atatttgatg tgctgggatt acaaaccata acagaagaag caggaagtga taaactggat gaaacattac agttattgat tgaactgaga aatcaggcaa gaaaaactaa aaactgggag ttatcggatc agataaggga ccgccttctg gaaaaaggta ttgaactaaa agacggaaga gaggggacat cttatacgat taactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for ElanA.00133.a.B1.PW38251
PURIFICATION DATe: 4/10/2017
CONCENTRATION: 29mg/ml
OBSERVED MW: 63kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ LKIYNSLTGE KEDFKPILDG NVGMYVCGPT VYSNVHLGNV RTFLSFDFIY RSLQFLGYKV RYVRNITDAG HLTDDGNIDN DRFIKQSRLE KLEPMEIVQK YTVDFHEVLK KFNLLPPTIE PTATGHIIEQ IELTKKLIEK GFAYESNGSV YFDVLEYNAR GLNYGELSRR NIEELFANTR DLDGQGEKKN PQDFALWKKA SPQHIMRWIS PWGEGFPGWH LECTAMSTKY LGDKFDIHGG GMDLKFPHHE CEIAQGKACN GTEPVNYWMH ANMLTMNGQR MSKSTGNYIL PMELITGNNN FFEKPFHPSV LRFCFLQAHY RSVLDISNEA MLASEKGFSR LMDAVKILDE IQPSDVSTVN VQEWLDKAYS ALVDDFNSPI LISHLFEAVK WIFILKDGKE TITANDLILL KEKLNAFIFD VLGLQTITEE AGSDKLDETL QLLIELRNQA RKTKNWELSD QIRDRLLEKG IELKDGR
Validated NT Sequence
ctctcttccg tcttttagtt caataccttt ttccagaagg cggtccctta tctgatccga taactcccag tttttagttt ttcttgcctg atttctcagt tcaatcaata actgtaatgt ttcatccagt ttatcacttc ctgcttcttc tgttatggtt tgtaatccca gcacatcaaa tataaaagca ttcagctttt ctttcagcaa tataaggtca tttgctgtaa ttgtttcttt cccatcttta agaatgaaaa tccattttac cgcttcaaaa agatgcgata taagtattgg actattgaaa tcgtcaacaa gcgcggaata agctttatcc aaccattcct gaacattaac cgtagaaaca tcagaaggtt ggatttcatc caatattttt acagcatcca tcaggcggct aaagcccttt tcacttgcca acattgcctc attggaaata tccagtacac ttctgtagtg tgcctgaagg aaacagaaac gcaacacgct tggatggaat ggtttctcga agaagttatt attcccggta attaactcca ttggcagaat ataattacct gtagatttac tcattcgctg cccattcatt gtcagcatat tggcatgcat ccagtagttt accggttctg taccattgca agcttttccc tgggcaatct cacattcatg gtgtggaaat ttaagatcca tcccaccacc atgaatatcg aatttatctc caagatattt agtactcatt gctgtacact ccaggtgcca ccccggaaaa ccttcccccc aaggagaaat ccaacgcata atatgttgtg gggatgcttt cttccaaagt gcaaaatcct gtggattttt tttctcaccc tggccatcca ggtctcgggt attggcaaaa agttcttcta tatttctgcg gcttagttct ccgtagttaa gtcctcttgc attatattcc aaaacatcga aataaaccga cccattgctt tcgtaagcaa atcccttttc aattaatttt tttgtcagtt caatctgctc gatgatatgt cctgtcgcag ttggctctat tgtaggcgga agcaggttaa atttctttag tacctcatga aaatcgactg tatacttctg tacaatttcc attggctcca gcttttccag cctggattgt ttaatgaaac gatcattatc tatgtttccg tcatctgtta aatgtcccgc atcagtaata tttcttacat accttacttt atatcccaga aattgcaggg atctatatat aaagtcaaag gaaagaaaag ttcttacatt ccctaaatga acattactat atactgtagg tccgcagaca tacattccca cgtttccgtc taatataggt ttgaagtctt ctttttctcc tgtaagcgag ttatatattt taagttgcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMQ LKIYNSLTGE KEDFKPILDG NVGMYVCGPT VYSNVHLGNV RTFLSFDFIY RSLQFLGYKV RYVRNITDAG HLTDDGNIDN DRFIKQSRLE KLEPMEIVQK YTVDFHEVLK KFNLLPPTIE PTATGHIIEQ IELTKKLIEK GFAYESNGSV YFDVLEYNAR GLNYGELSRR NIEELFANTR DLDGQGEKKN PQDFALWKKA SPQHIMRWIS PWGEGFPGWH LECTAMSTKY LGDKFDIHGG GMDLKFPHHE CEIAQGKACN GTEPVNYWMH ANMLTMNGQR MSKSTGNYIL PMELITGNNN FFEKPFHPSV LRFCFLQAHY RSVLDISNEA MLASEKGFSR LMDAVKILDE IQPSDVSTVN VQEWLDKAYS ALVDDFNSPI LISHLFEAVK WIFILKDGKE TITANDLILL KEKLNAFIFD VLGLQTITEE AGSDKLDETL QLLIELRNQA RKTKNWELSD QIRDRLLEKG IELKDGREGT SYTIN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAC TTAAAATATA TAACTCGCTT ACAGGAGAAA AAGAAGACTT CAAACCTATA TTAGACGGAA ACGTGGGAAT GTATGTCTGC GGACCTACAG TATATAGTAA TGTTCATTTA GGGAATGTAA GAACTTTTCT TTCCTTTGAC TTTATATATA GATCCCTGCA ATTTCTGGGA TATAAAGTAA GGTATGTAAG AAATATTACT GATGCGGGAC ATTTAACAGA TGACGGAAAC ATAGATAATG ATCGTTTCAT TAAACAATCC AGGCTGGAAA AGCTGGAGCC AATGGAAATT GTACAGAAGT ATACAGTCGA TTTTCATGAG GTACTAAAGA AATTTAACCT GCTTCCGCCT ACAATAGAGC CAACTGCGAC AGGACATATC ATCGAGCAGA TTGAACTGAC AAAAAAATTA ATTGAAAAGG GATTTGCTTA CGAAAGCAAT GGGTCGGTTT ATTTCGATGT TTTGGAATAT AATGCAAGAG GACTTAACTA CGGAGAACTA AGCCGCAGAA ATATAGAAGA ACTTTTTGCC AATACCCGAG ACCTGGATGG CCAGGGTGAG AAAAAAAATC CACAGGATTT TGCACTTTGG AAGAAAGCAT CCCCACAACA TATTATGCGT TGGATTTCTC CTTGGGGGGA AGGTTTTCCG GGGTGGCACC TGGAGTGTAC AGCAATGAGT ACTAAATATC TTGGAGATAA ATTCGATATT CATGGTGGTG GGATGGATCT TAAATTTCCA CACCATGAAT GTGAGATTGC CCAGGGAAAA GCTTGCAATG GTACAGAACC GGTAAACTAC TGGATGCATG CCAATATGCT GACAATGAAT GGGCAGCGAA TGAGTAAATC TACAGGTAAT TATATTCTGC CAATGGAGTT AATTACCGGG AATAATAACT TCTTCGAGAA ACCATTCCAT CCAAGCGTGT TGCGTTTCTG TTTCCTTCAG GCACACTACA GAAGTGTACT GGATATTTCC AATGAGGCAA TGTTGGCAAG TGAAAAGGGC TTTAGCCGCC TGATGGATGC TGTAAAAATA TTGGATGAAA TCCAACCTTC TGATGTTTCT ACGGTTAATG TTCAGGAATG GTTGGATAAA GCTTATTCCG CGCTTGTTGA CGATTTCAAT AGTCCAATAC TTATATCGCA TCTTTTTGAA GCGGTAAAAT GGATTTTCAT TCTTAAAGAT GGGAAAGAAA CAATTACAGC AAATGACCTT ATATTGCTGA AAGAAAAGCT GAATGCTTTT ATATTTGATG TGCTGGGATT ACAAACCATA ACAGAAGAAG CAGGAAGTGA TAAACTGGAT GAAACATTAC AGTTATTGAT TGAACTGAGA AATCAGGCAA GAAAAACTAA AAACTGGGAG TTATCGGATC AGATAAGGGA CCGCCTTCTG GAAAAAGGTA TTGAACTAAA AGACGGAAGA GAGGGGACAT CTTATACGAT TAACTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT