ElanA.00121.a

Biotin synthase

CENTER ID: ElanA.00121.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00121.a.B1.GE40575 Full length(ElanA.00121.a) 1 331
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.2368
UniProt: A0A077EBD1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKERTLRHD WTKEELLEIY NKPLMELIYE AATVHREWHK ADEVQISTLL SVKTGGCPED CSYCGQAARY HTDIKVQALL PTATVMAHAQ KAKERGASRF CMAAAWREVR DNRDFDRIID MVKGVNDLGL EVCCTLGMLS ESQAKRLQEA GLYAYNHNLD TSEEYYDEII STRKFDNRIN TINNVRKAGI TVCSGGIIGL GETHGDRVAM LRTLANMEIH PESVPVNALA RVKGTPLENN PKVDIWEMVR MIATARISMP ASMVRLSAGR IEMTEAEQAW CFMAGANSIF TGERETLLVT PNPGVSEDMQ MLLNLGLKPM VREKETTCSK N
NT Sequence
ATGGCTAAAG AAAGAACATT GCGTCACGAC TGGACAAAAG AAGAATTACT GGAGATTTAT AATAAACCAT TAATGGAGCT TATTTATGAA GCTGCAACAG TACATCGTGA ATGGCATAAA GCAGATGAAG TACAGATATC AACATTGTTG TCAGTAAAAA CAGGGGGCTG TCCTGAGGAT TGTTCTTATT GTGGACAGGC AGCGCGCTAT CATACAGATA TTAAAGTACA GGCACTTTTA CCTACAGCAA CCGTAATGGC CCATGCACAA AAAGCTAAAG AAAGAGGAGC TTCTCGCTTC TGCATGGCAG CTGCATGGCG TGAAGTAAGA GATAACAGAG ATTTTGACCG TATTATAGAT ATGGTAAAAG GGGTGAACGA TCTGGGGTTG GAAGTTTGCT GTACTTTGGG TATGCTTAGT GAGTCACAGG CTAAAAGACT ACAGGAAGCA GGTCTATATG CTTACAATCA TAATCTGGAT ACCTCTGAGG AATATTATGA TGAGATTATT TCCACACGAA AATTTGATAA CCGTATCAAT ACGATAAACA ATGTGAGAAA AGCAGGAATT ACGGTATGTT CCGGTGGTAT TATCGGCTTA GGCGAAACTC ATGGAGACCG CGTGGCAATG CTGAGAACTT TGGCTAATAT GGAGATACAT CCCGAATCGG TTCCTGTAAA TGCTTTGGCA AGAGTAAAGG GAACACCATT GGAAAATAAT CCAAAAGTGG ATATATGGGA AATGGTAAGA ATGATAGCTA CAGCCCGTAT TTCGATGCCT GCTTCTATGG TGCGCCTTAG CGCCGGACGT ATAGAAATGA CAGAAGCCGA GCAGGCATGG TGTTTTATGG CCGGAGCTAA CTCTATTTTT ACCGGTGAGC GGGAGACATT GTTGGTAACT CCAAATCCGG GTGTATCGGA AGATATGCAG ATGCTTCTGA ATTTAGGACT GAAACCAATG GTACGAGAAA AAGAAACGAC ATGCAGCAAG AAT
Details for ElanA.00121.a.B1.GE40575
HARVESTED ON: data unavailable
SEQUENCED ON: 10/19/2016
EXPECTED MW: 38kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA KERTLRHDWT KEELLEIYNK PLMELIYEAA TVHREWHKAD EVQISTLLSV KTGGCPEDCS YCGQAARYHT DIKVQALLPT ATVMAHAQKA KERGASRFCM AAAWREVRDN RDFDRIIDMV KGVNDLGLEV CCTLGMLSES QAKRLQEAGL YAYNHNLDTS EEYYDEIIST RKFDNRINTI NNVRKAGITV CSGGIIGLGE THGDRVAMLR TLANMEIHPE SVPVNALARV KGTPLENNPK VDIWEMVRMI ATARISMPAS MVRLSAGRIE MTEAEQAWCF MAGANSIFTG ERETLLVTPN PGVSEDMQML LNLGLKPMVR E
Validated NT Sequence
tttttctcgt accattggtt tcagtcctaa attcagaagc atctgcatat cttccgatac acccggattt ggagttacca acaatgtctc ccgctcaccg gtaaaaatag agttagctcc ggccataaaa caccatgcct gctcggcttc tgtcatttct atacgtccgg cgctaaggcg caccatagaa gcaggcatcg aaatacgggc tgtagctatc attcttacca tttcccatat atccactttt ggattatttt ccaatggtgt tccctttact cttgccaaag catttacagg aaccgattcg ggatgtatct ccatattagc caaagttctc agcattgcca cgcggtctcc atgagtttcg cctaagccga taataccacc ggaacatacc gtaattcctg cttttctcac attgtttatc gtattgatac ggttatcaaa ttttcgtgtg gaaataatct catcataata ttcctcagag gtatccagat tatgattgta agcatataga cctgcttcct gtagtctttt agcctgtgac tcactaagca tacccaaagt acagcaaact tccaacccca gatcgttcac cccttttacc atatctataa tacggtcaaa atctctgtta tctcttactt cacgccatgc agctgccatg cagaagcgag aagctcctct ttctttagct ttttgtgcat gggccattac ggttgctgta ggtaaaagtg cctgtacttt aatatctgta tgatagcgcg ctgcctgtcc acaataagaa caatcctcag gacagccccc tgtttttact gacaacaatg ttgatatctg tacttcatct gctttatgcc attcacgatg tactgttgca gcttcataaa taagctccat taatggttta ttataaatct ccagtaattc ttcttttgtc cagtcgtgac gcaatgttct ttctttagcc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMA KERTLRHDWT KEELLEIYNK PLMELIYEAA TVHREWHKAD EVQISTLLSV KTGGCPEDCS YCGQAARYHT DIKVQALLPT ATVMAHAQKA KERGASRFCM AAAWREVRDN RDFDRIIDMV KGVNDLGLEV CCTLGMLSES QAKRLQEAGL YAYNHNLDTS EEYYDEIIST RKFDNRINTI NNVRKAGITV CSGGIIGLGE THGDRVAMLR TLANMEIHPE SVPVNALARV KGTPLENNPK VDIWEMVRMI ATARISMPAS MVRLSAGRIE MTEAEQAWCF MAGANSIFTG ERETLLVTPN PGVSEDMQML LNLGLKPMVR EKETTCSKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcta aagaaagaac attgcgtcac gactggacaa aagaagaatt actggagatt tataataaac cattaatgga gcttatttat gaagctgcaa cagtacatcg tgaatggcat aaagcagatg aagtacagat atcaacattg ttgtcagtaa aaacaggggg ctgtcctgag gattgttctt attgtggaca ggcagcgcgc tatcatacag atattaaagt acaggcactt ttacctacag caaccgtaat ggcccatgca caaaaagcta aagaaagagg agcttctcgc ttctgcatgg cagctgcatg gcgtgaagta agagataaca gagattttga ccgtattata gatatggtaa aaggggtgaa cgatctgggg ttggaagttt gctgtacttt gggtatgctt agtgagtcac aggctaaaag actacaggaa gcaggtctat atgcttacaa tcataatctg gatacctctg aggaatatta tgatgagatt atttccacac gaaaatttga taaccgtatc aatacgataa acaatgtgag aaaagcagga attacggtat gttccggtgg tattatcggc ttaggcgaaa ctcatggaga ccgcgtggca atgctgagaa ctttggctaa tatggagata catcccgaat cggttcctgt aaatgctttg gcaagagtaa agggaacacc attggaaaat aatccaaaag tggatatatg ggaaatggta agaatgatag ctacagcccg tatttcgatg cctgcttcta tggtgcgcct tagcgccgga cgtatagaaa tgacagaagc cgagcaggca tggtgtttta tggccggagc taactctatt tttaccggtg agcgggagac attgttggta actccaaatc cgggtgtatc ggaagatatg cagatgcttc tgaatttagg actgaaacca atggtacgag aaaaagaaac gacatgcagc aagaattgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t