SSGCID: Target Page

ElanA.00085.c

GDP-mannose 4,6-dehydratase

CENTER ID:	ElanA.00085.c
ORGANISM:	Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.00085.c.B1.GE40572	Full length(ElanA.00085.c)		1	352

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1338011.4.peg.3353
UniProt:	A0A077EI09

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKTALITGI TGQDGSFLAE FLIEKGYEVH GIIRRSSSFN TDRIEHLYFD EWVRDMHQQR LINLHYGDMT DSSSLMRIIQ ITQPDEIYNL AAQSHVKVSF DVPEYTAETD AVGTLRLLEA VRILGMENKT RIYQASTSEL FGLVQEVPQK ETTPFYPRSP YGVAKQYGFW ITKNYRESYG MYAVNGILFN HESERRGETF VTRKVTLAVG RIVAGTQEKL YMGNLDALRD WGYAKDYVEC MWLMLQHDVP EDFVIATGEM HTVREFITKS FKVAGIEIVW EGTGENEKGI DKATGKILVE VDPKYFRPAE VELLLGDPTK AKTLLGWNPT KTSFDELVEI MVKHDLALAQ KK

NT Sequence

ATGAAAAAAA CTGCATTAAT TACAGGTATA ACAGGACAAG ATGGCTCCTT CTTAGCAGAA TTTCTAATTG AAAAAGGATA TGAAGTACAT GGTATTATAA GAAGGTCATC TTCTTTTAAT ACTGATCGCA TAGAGCATTT ATATTTTGAT GAATGGGTTC GAGATATGCA TCAACAAAGG CTTATTAATC TTCATTATGG TGATATGACT GATTCAAGTT CTTTGATGCG TATTATTCAA ATTACTCAGC CAGATGAAAT TTATAACCTT GCTGCGCAAA GTCACGTTAA AGTGAGTTTT GATGTGCCAG AATATACAGC AGAAACAGAT GCTGTTGGTA CCCTTAGACT TTTGGAGGCA GTAAGAATTC TTGGAATGGA GAATAAAACA AGGATTTATC AAGCCTCAAC ATCAGAACTT TTTGGTTTGG TACAAGAAGT TCCTCAAAAG GAAACAACCC CTTTCTATCC ACGTTCACCT TACGGAGTAG CTAAGCAATA TGGTTTTTGG ATTACAAAGA ATTACAGGGA GTCATATGGA ATGTATGCCG TAAATGGTAT ACTATTCAAC CATGAAAGTG AAAGAAGAGG AGAAACGTTT GTTACAAGAA AAGTAACATT GGCGGTCGGT AGAATTGTCG CTGGTACACA AGAAAAGCTT TACATGGGGA ATTTAGATGC GTTAAGAGAC TGGGGGTATG CAAAAGATTA TGTGGAATGT ATGTGGTTAA TGTTACAACA TGATGTTCCA GAAGACTTTG TTATTGCAAC AGGTGAAATG CATACTGTAA GAGAATTTAT TACCAAATCC TTTAAAGTAG CTGGTATTGA AATCGTTTGG GAAGGTACAG GCGAAAATGA GAAAGGTATT GATAAAGCTA CAGGAAAAAT ATTGGTGGAA GTTGATCCTA AGTATTTCCG TCCTGCTGAA GTGGAACTTT TACTTGGAGA CCCTACAAAA GCGAAGACTT TATTAGGTTG GAATCCTACA AAAACGAGTT TTGATGAGTT GGTTGAAATA ATGGTAAAAC ATGATCTAGC ATTAGCTCAA AAAAAA

Details for ElanA.00085.c.B1.GE40572

HARVESTED ON:	10/18/2016
SEQUENCED ON:	10/19/2016
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMK KTALITGITG QDGSFLAEFL IEKGYEVHGI IRRSSSFNTD RIEHLYFDEW VRDMHQQRLI NLHYGDMTDS SSLMRIIQIT QPDEIYNLAA QSHVKVSFDV PEYTAETDAV GTLRLLEAVR ILGMENKTRI YQASTSELFG LVQEVPQKET TPFYPRSPYG VAKQYGFWIT KNYRESYGMY AVNGILFNHE SERRGETFVT RKVTLAVGRI VAGTQEKLYM GNLDALRDWG YAKDYVECMW LMLQHDVPED FVIATGEMHT VREFITKSFK VAGIEIVWEG TGENEKGIDK ATGKILVEVD PKYFRPAEVE LLLGDPTKAK TLLGWNPTKT SFDELVEIMV KH

Validated NT Sequence

tcatgtttta ccattatttc aaccaactca tcaaaactcg tttttgtagg attccaacct aataaagtct tcgcttttgt agggtctcca agtaaaagtt ccacttcagc aggacggaaa tacttaggat caacttccac caatattttt cctgtagctt tatcaatacc tttctcattt tcgcctgtac cttcccaaac gatttcaata ccagctactt taaaggattt ggtaataaat tctcttacag tatgcatttc acctgttgca ataacaaagt cttctggaac atcatgttgt aacattaacc acatacattc cacataatct tttgcatacc cccagtctct taacgcatct aaattcccca tgtaaagctt ttcttgtgta ccagcgacaa ttctaccgac cgccaatgtt acttttcttg taacaaacgt ttctcctctt ctttcacttt catggttgaa tagtatacca tttacggcat acattccata tgactccctg taattctttg taatccaaaa accatattgc ttagctactc cgtaaggtga acgtggatag aaaggggttg tttccttttg aggaacttct tgtaccaaac caaaaagttc tgatgttgag gcttgataaa tccttgtttt attctccatt ccaagaattc ttactgcctc caaaagtcta agggtaccaa cagcatctgt ttctgctgta tattctggca catcaaaact cactttaacg tgactttgcg cagcaaggtt ataaatttca tctggctgag taatttgaat aatacgcatc aaagaacttg aatcagtcat atcaccataa tgaagattaa taagcctttg ttgatgcata tctcgaaccc attcatcaaa atataaatgc tctatgcgat cagtattaaa agaagatgac cttcttataa taccatgtac ttcatatcct ttttcaatta gaaattctgc taagaaggag ccatcttgtc ctgttatacc tgtaattaat gcagtttttt tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa aaactgcatt aattacaggt ataacaggac aagatggctc cttcttagca gaatttctaa ttgaaaaagg atatgaagta catggtatta taagaaggtc atcttctttt aatactgatc gcatagagca tttatatttt gatgaatggg ttcgagatat gcatcaacaa aggcttatta atcttcatta tggtgatatg actgattcaa gttctttgat gcgtattatt caaattactc agccagatga aatttataac cttgctgcgc aaagtcacgt taaagtgagt tttgatgtgc cagaatatac agcagaaaca gatgctgttg gtacccttag acttttggag gcagtaagaa ttcttggaat ggagaataaa acaaggattt atcaagcctc aacatcagaa ctttttggtt tggtacaaga agttcctcaa aaggaaacaa cccctttcta tccacgttca ccttacggag tagctaagca atatggtttt tggattacaa agaattacag ggagtcatat ggaatgtatg ccgtaaatgg tatactattc aaccatgaaa gtgaaagaag aggagaaacg tttgttacaa gaaaagtaac attggcggtc ggtagaattg tcgctggtac acaagaaaag ctttacatgg ggaatttaga tgcgttaaga gactgggggt atgcaaaaga ttatgtggaa tgtatgtggt taatgttaca acatgatgtt ccagaagact ttgttattgc aacaggtgaa atgcatactg taagagaatt tattaccaaa tcctttaaag tagctggtat tgaaatcgtt tgggaaggta caggcgaaaa tgagaaaggt attgataaag ctacaggaaa aatattggtg gaagttgatc ctaagtattt ccgtcctgct gaagtggaac ttttacttgg agaccctaca aaagcgaaga ctttattagg ttggaatcct acaaaaacga gttttgatga gttggttgaa ataatggtaa aacatgatct agcattagct caaaaaaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat