ElanA.00085.b

UDP-glucose 4-epimerase

CENTER ID: ElanA.00085.b
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00085.b.B1.GE40571 Full length(ElanA.00085.b) 1 338
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.929
UniProt: A0A077EE56

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAILVTGGLG YIGSHTVVEL IQNNFDVVII DDLSNTEKRV LDGIEKITGV RPTFYPFDLK RRELLSQVLE AHKIEGCIHF AAYKAVGESQ EKPVDYYENN LFSLINILQE FKERNISNFI FSSSCTVYGQ ADEMPINEET VLKLPESSYG KTKQMGEEII KDFAIAHHKK VSLLRYFNPI GAHPSALIGE LPLGIPNNLI PYVSQTAAGV REYLSIFGND YPTPDGTAVR DYIYVVDLAK AHVASLKKLI ASETDTVVDI YNLGTGKGSS VLEVVKSFET ANNVEVKYEF KPRRDGDITI AYANASKAEA ELGWKADTSL TEALKTTWQW QKHLESRD
NT Sequence
ATGGCAATAT TAGTAACAGG AGGACTGGGG TATATAGGTT CGCATACAGT AGTAGAACTT ATCCAGAACA ACTTTGATGT TGTGATTATC GATGATCTTT CCAATACCGA GAAAAGAGTT CTGGATGGCA TTGAGAAAAT TACCGGAGTA AGACCAACAT TCTATCCTTT TGATTTAAAA AGAAGAGAGC TGTTATCTCA GGTTTTAGAA GCTCATAAAA TTGAAGGATG TATTCATTTT GCAGCTTATA AAGCAGTAGG AGAAAGTCAG GAAAAACCAG TTGATTATTA TGAGAATAAT CTTTTTTCTC TGATTAATAT TCTTCAGGAA TTTAAAGAAA GAAATATTTC TAATTTTATT TTCAGCTCAT CTTGTACTGT TTATGGACAA GCGGATGAAA TGCCTATTAA TGAAGAAACA GTGCTGAAAT TACCTGAATC TTCTTATGGT AAGACAAAGC AAATGGGAGA GGAGATTATC AAAGATTTTG CAATTGCTCA TCATAAAAAA GTAAGTTTAC TAAGATATTT CAATCCTATT GGTGCTCATC CAAGTGCTTT AATCGGTGAG TTACCTCTTG GTATTCCAAA TAATCTTATC CCTTATGTTA GCCAGACTGC AGCAGGTGTT CGCGAGTATT TAAGTATTTT CGGAAACGAT TATCCGACAC CAGATGGCAC TGCTGTTCGT GATTATATAT ATGTAGTAGA CTTAGCAAAA GCACATGTTG CTTCTCTTAA AAAATTAATT GCTTCAGAAA CAGATACTGT TGTAGATATC TATAACCTTG GTACAGGTAA AGGATCGTCT GTATTAGAAG TTGTTAAAAG CTTTGAAACG GCTAATAATG TAGAAGTAAA ATATGAGTTT AAACCACGTC GTGACGGTGA TATTACTATA GCTTATGCAA ATGCCTCAAA AGCAGAGGCA GAGCTAGGCT GGAAAGCTGA TACAAGCCTT ACAGAAGCCC TGAAAACAAC ATGGCAGTGG CAAAAACATT TGGAAAGTAG AGAT
Details for ElanA.00085.b.B1.GE40571
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA ILVTGGLGYI GSHTVVELIQ NNFDVVIIDD LSNTEKRVLD GIEKITGVRP TFYPFDLKRR ELLSQVLEAH KIEGCIHFAA YKAVGESQEK PVDYYENNLF SLINILQEFK ERNISNFIFS SSCTVYGQAD EMPINEETVL KLPESSYGKT KQMGEEIIKD FAIAHHKKVS LLRYFNPIGA HPSALIGELP LGIPNNLIPY VSQTAAGVRE YLSIFGNDYP TPDGTAVRDY IYVVDLAKAH VASLKKLIAS ETDTVVDIYN LGTGKGSSVL EVVKSFETAN NVEVKYEFKP RRDGDITIAY ANASKAEAEL GWKADTSLTE ALKTTWQWQ
Validated NT Sequence
tttgccactg ccatgttgtt ttcagggctt ctgtaaggct tgtatcagct ttccagccta gctctgcctc tgcttttgag gcatttgcat aagctatagt aatatcaccg tcacgacgtg gtttaaactc atattttact tctacattat tagccgtttc aaagctttta acaacttcta atacagacga tcctttacct gtaccaaggt tatagatatc tacaacagta tctgtttctg aagcaattaa ttttttaaga gaagcaacat gtgcttttgc taagtctact acatatatat aatcacgaac agcagtgcca tctggtgtcg gataatcgtt tccgaaaata cttaaatact cgcgaacacc tgctgcagtc tggctaacat aagggataag attatttgga ataccaagag gtaactcacc gattaaagca cttggatgag caccaatagg attgaaatat cttagtaaac ttactttttt atgatgagca attgcaaaat ctttgataat ctcctctccc atttgctttg tcttaccata agaagattca ggtaatttca gcactgtttc ttcattaata ggcatttcat ccgcttgtcc ataaacagta caagatgagc tgaaaataaa attagaaata tttctttctt taaattcctg aagaatatta atcagagaaa aaagattatt ctcataataa tcaactggtt tttcctgact ttctcctact gctttataag ctgcaaaatg aatacatcct tcaattttat gagcttctaa aacctgagat aacagctctc ttctttttaa atcaaaagga tagaatgttg gtcttactcc ggtaattttc tcaatgccat ccagaactct tttctcggta ttggaaagat catcgataat cacaacatca aagttgttct ggataagttc tactactgta tgcgaaccta tataccccag tcctcctgtt actaatattg ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMA ILVTGGLGYI GSHTVVELIQ NNFDVVIIDD LSNTEKRVLD GIEKITGVRP TFYPFDLKRR ELLSQVLEAH KIEGCIHFAA YKAVGESQEK PVDYYENNLF SLINILQEFK ERNISNFIFS SSCTVYGQAD EMPINEETVL KLPESSYGKT KQMGEEIIKD FAIAHHKKVS LLRYFNPIGA HPSALIGELP LGIPNNLIPY VSQTAAGVRE YLSIFGNDYP TPDGTAVRDY IYVVDLAKAH VASLKKLIAS ETDTVVDIYN LGTGKGSSVL EVVKSFETAN NVEVKYEFKP RRDGDITIAY ANASKAEAEL GWKADTSLTE ALKTTWQWQK HLESRD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa tattagtaac aggaggactg gggtatatag gttcgcatac agtagtagaa cttatccaga acaactttga tgttgtgatt atcgatgatc tttccaatac cgagaaaaga gttctggatg gcattgagaa aattaccgga gtaagaccaa cattctatcc ttttgattta aaaagaagag agctgttatc tcaggtttta gaagctcata aaattgaagg atgtattcat tttgcagctt ataaagcagt aggagaaagt caggaaaaac cagttgatta ttatgagaat aatctttttt ctctgattaa tattcttcag gaatttaaag aaagaaatat ttctaatttt attttcagct catcttgtac tgtttatgga caagcggatg aaatgcctat taatgaagaa acagtgctga aattacctga atcttcttat ggtaagacaa agcaaatggg agaggagatt atcaaagatt ttgcaattgc tcatcataaa aaagtaagtt tactaagata tttcaatcct attggtgctc atccaagtgc tttaatcggt gagttacctc ttggtattcc aaataatctt atcccttatg ttagccagac tgcagcaggt gttcgcgagt atttaagtat tttcggaaac gattatccga caccagatgg cactgctgtt cgtgattata tatatgtagt agacttagca aaagcacatg ttgcttctct taaaaaatta attgcttcag aaacagatac tgttgtagat atctataacc ttggtacagg taaaggatcg tctgtattag aagttgttaa aagctttgaa acggctaata atgtagaagt aaaatatgag tttaaaccac gtcgtgacgg tgatattact atagcttatg caaatgcctc aaaagcagag gcagagctag gctggaaagc tgatacaagc cttacagaag ccctgaaaac aacatggcag tggcaaaaac atttggaaag tagagattga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at