ElanA.00085.a
dTDP-glucose 4,6-dehydratase
| CENTER ID: | ElanA.00085.a |
| ORGANISM: | Elizabethkingia anophelis NUHP1 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.00085.a.B1.GE40570 | Full length(ElanA.00085.a) | 1 | 359 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.00085.a.B1.PS38379 | Full length(ElanA.00085.a) | 1 | 359 | ||
| ElanA.00085.a.B1.PS38380 | Full length(ElanA.00085.a) | 1 | 359 |
Structures
8DU1
DEPOSITED: 7/26/2022
DETERMINATION: XRay
CLONE: ElanA.00085.a.B1.GE40570
PROTEIN: ElanA.00085.a.B1.PS38380
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|1338011.4.peg.3335 |
| UniProt: | A0A077ELH2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKNIIITGGA GFIGSHVVRE FVIKNPEITI INLDALTYAG NLENLKDIEN FPNYVFEKAD ITKPEELRKV FEKYNPDAVV HLAAESHVDR SITDPNAFIN TNVIGTANLL NLCREFWTLN PEHTHGRFPN EPRTNLFYHV STDEVYGSLG ETGFFLETTA YDPQSPYSAS KAASDHLVRA YGNTYGMPFI VSNCSNNYGP NHFPEKLIPL CISNILNEKP LPIYGDGKYT RDWLYVIDHA RAIHQIFNEA KTGETYNIGG FNEWQNIDLV KELIKQLDAK LGKPEGHSEK LITFVKDRPG HDKRYAIDAT KLNKDLGWKP SVTFEEGLAK TIDWYLDNKE WLENVTSGDY QKYYENQYS
NT Sequence
ATGAAGAATA TAATCATTAC CGGAGGGGCG GGTTTTATTG GCTCCCATGT TGTAAGAGAG TTTGTGATAA AAAATCCTGA GATTACGATT ATTAATCTCG ATGCATTGAC GTATGCAGGA AACCTGGAAA ATTTGAAAGA TATTGAAAAT TTTCCTAATT ATGTTTTCGA AAAAGCAGAT ATTACAAAAC CTGAAGAATT AAGAAAGGTT TTTGAAAAAT ACAATCCGGA TGCTGTAGTA CATTTAGCAG CAGAGAGCCA TGTAGACAGA AGTATTACAG ACCCAAATGC ATTTATCAAT ACAAATGTTA TCGGAACAGC TAACTTATTG AACTTATGTC GTGAATTTTG GACACTGAAT CCAGAACATA CGCATGGCAG ATTCCCAAAT GAACCAAGGA CAAATCTTTT TTACCACGTA TCTACAGATG AAGTATATGG AAGTTTAGGC GAAACAGGTT TCTTTTTAGA AACAACAGCC TATGACCCTC AATCTCCTTA TTCCGCTTCC AAAGCAGCGT CCGATCATTT GGTAAGAGCT TACGGTAATA CCTATGGAAT GCCATTTATA GTTTCTAATT GTTCCAATAA TTATGGACCA AACCACTTCC CTGAAAAGTT GATTCCTTTG TGTATTTCTA ATATTTTAAA TGAAAAGCCA TTGCCAATAT ATGGAGATGG AAAATATACA AGAGATTGGT TGTATGTAAT TGACCATGCC AGAGCAATTC ATCAAATATT TAATGAAGCT AAAACTGGAG AAACTTATAA TATTGGAGGT TTTAACGAGT GGCAGAATAT TGATCTGGTA AAAGAATTAA TCAAGCAATT AGATGCTAAA TTAGGAAAGC CTGAAGGTCA TTCTGAAAAG CTGATTACAT TTGTTAAAGA CCGCCCGGGG CATGATAAGC GTTATGCAAT TGATGCGACG AAATTAAATA AAGATTTAGG CTGGAAACCC TCCGTTACTT TTGAAGAAGG ATTAGCTAAG ACAATTGATT GGTATTTAGA TAATAAAGAG TGGCTAGAGA ATGTAACATC TGGTGACTAT CAAAAATATT ATGAAAATCA ATATTCT
Details for ElanA.00085.a.B1.GE40570
| HARVESTED ON: | 10/18/2016 |
| SEQUENCED ON: | 10/19/2016 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 42kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMK NIIITGGAGF IGSHVVREFV IKNPEITIIN LDALTYAGNL ENLKDIENFP NYVFEKADIT KPEELRKVFE KYNPDAVVHL AAESHVDRSI TDPNAFINTN VIGTANLLNL CREFWTLNPE HTHGRFPNEP RTNLFYHVST DEVYGSLGET GFFLETTAYD PQSPYSASKA ASDHLVRAYG NTYGMPFIVS NCSNNYGPNH FPEKLIPLCI SNILNEKPLP IYGDGKYTRD WLYVIDHARA IHQIFNEAKT GETYNIGGFN EWQNIDLVKE LIKQLDAKLG KPEGHSEKLI TFVKDRPGHD KRYAIDATKL NKDLGWKPSV TFEEGLAKTI DWYLDNKEWL ENVTSGDYQ
Validated NT Sequence
tttttgatag tcaccagatg ttacattctc tagccactct ttattatcta aataccaatc aattgtctta gctaatcctt cttcaaaagt aacggagggt ttccagccta aatctttatt taatttcgtc gcatcaattg cataacgctt atcatgcccc gggcggtctt taacaaatgt aatcagcttt tcagaatgac cttcaggctt tcctaattta gcatctaatt gcttgattaa ttcttttacc agatcaatat tctgccactc gttaaaacct ccaatattat aagtttctcc agttttagct tcattaaata tttgatgaat tgctctggca tggtcaatta catacaacca atctcttgta tattttccat ctccatatat tggcaatggc ttttcattta aaatattaga aatacacaaa ggaatcaact tttcagggaa gtggtttggt ccataattat tggaacaatt agaaactata aatggcattc cataggtatt accgtaagct cttaccaaat gatcggacgc tgctttggaa gcggaataag gagattgagg gtcataggct gttgtttcta aaaagaaacc tgtttcgcct aaacttccat atacttcatc tgtagatacg tggtaaaaaa gatttgtcct tggttcattt gggaatctgc catgcgtatg ttctggattc agtgtccaaa attcacgaca taagttcaat aagttagctg ttccgataac atttgtattg ataaatgcat ttgggtctgt aatacttctg tctacatggc tctctgctgc taaatgtact acagcatccg gattgtattt ttcaaaaacc tttcttaatt cttcaggttt tgtaatatct gctttttcga aaacataatt aggaaaattt tcaatatctt tcaaattttc caggtttcct gcatacgtca atgcatcgag attaataatc gtaatctcag gattttttat cacaaactct cttacaacat gggagccaat aaaacccgcc cctccggtaa tgattatatt cttcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMK NIIITGGAGF IGSHVVREFV IKNPEITIIN LDALTYAGNL ENLKDIENFP NYVFEKADIT KPEELRKVFE KYNPDAVVHL AAESHVDRSI TDPNAFINTN VIGTANLLNL CREFWTLNPE HTHGRFPNEP RTNLFYHVST DEVYGSLGET GFFLETTAYD PQSPYSASKA ASDHLVRAYG NTYGMPFIVS NCSNNYGPNH FPEKLIPLCI SNILNEKPLP IYGDGKYTRD WLYVIDHARA IHQIFNEAKT GETYNIGGFN EWQNIDLVKE LIKQLDAKLG KPEGHSEKLI TFVKDRPGHD KRYAIDATKL NKDLGWKPSV TFEEGLAKTI DWYLDNKEWL ENVTSGDYQK YYENQYS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga atataatcat taccggaggg gcgggtttta ttggctccca tgttgtaaga gagtttgtga taaaaaatcc tgagattacg attattaatc tcgatgcatt gacgtatgca ggaaacctgg aaaatttgaa agatattgaa aattttccta attatgtttt cgaaaaagca gatattacaa aacctgaaga attaagaaag gtttttgaaa aatacaatcc ggatgctgta gtacatttag cagcagagag ccatgtagac agaagtatta cagacccaaa tgcatttatc aatacaaatg ttatcggaac agctaactta ttgaacttat gtcgtgaatt ttggacactg aatccagaac atacgcatgg cagattccca aatgaaccaa ggacaaatct tttttaccac gtatctacag atgaagtata tggaagttta ggcgaaacag gtttcttttt agaaacaaca gcctatgacc ctcaatctcc ttattccgct tccaaagcag cgtccgatca tttggtaaga gcttacggta atacctatgg aatgccattt atagtttcta attgttccaa taattatgga ccaaaccact tccctgaaaa gttgattcct ttgtgtattt ctaatatttt aaatgaaaag ccattgccaa tatatggaga tggaaaatat acaagagatt ggttgtatgt aattgaccat gccagagcaa ttcatcaaat atttaatgaa gctaaaactg gagaaactta taatattgga ggttttaacg agtggcagaa tattgatctg gtaaaagaat taatcaagca attagatgct aaattaggaa agcctgaagg tcattctgaa aagctgatta catttgttaa agaccgcccg gggcatgata agcgttatgc aattgatgcg acgaaattaa ataaagattt aggctggaaa ccctccgtta cttttgaaga aggattagct aagacaattg attggtattt agataataaa gagtggctag agaatgtaac atctggtgac tatcaaaaat attatgaaaa tcaatattct tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for ElanA.00085.a.B1.PS38379
| PURIFICATION DATe: | 11/6/2017 |
| CONCENTRATION: | 10.57mg/ml |
| OBSERVED MW: | 43kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK NIIITGGAGF IGSHVVREFV IKNPEITIIN LDALTYAGNL ENLKDIENFP NYVFEKADIT KPEELRKVFE KYNPDAVVHL AAESHVDRSI TDPNAFINTN VIGTANLLNL CREFWTLNPE HTHGRFPNEP RTNLFYHVST DEVYGSLGET GFFLETTAYD PQSPYSASKA ASDHLVRAYG NTYGMPFIVS NCSNNYGPNH FPEKLIPLCI SNILNEKPLP IYGDGKYTRD WLYVIDHARA IHQIFNEAKT GETYNIGGFN EWQNIDLVKE LIKQLDAKLG KPEGHSEKLI TFVKDRPGHD KRYAIDATKL NKDLGWKPSV TFEEGLAKTI DWYLDNKEWL ENVTSGDYQ
Validated NT Sequence
tttttgatag tcaccagatg ttacattctc tagccactct ttattatcta aataccaatc aattgtctta gctaatcctt cttcaaaagt aacggagggt ttccagccta aatctttatt taatttcgtc gcatcaattg cataacgctt atcatgcccc gggcggtctt taacaaatgt aatcagcttt tcagaatgac cttcaggctt tcctaattta gcatctaatt gcttgattaa ttcttttacc agatcaatat tctgccactc gttaaaacct ccaatattat aagtttctcc agttttagct tcattaaata tttgatgaat tgctctggca tggtcaatta catacaacca atctcttgta tattttccat ctccatatat tggcaatggc ttttcattta aaatattaga aatacacaaa ggaatcaact tttcagggaa gtggtttggt ccataattat tggaacaatt agaaactata aatggcattc cataggtatt accgtaagct cttaccaaat gatcggacgc tgctttggaa gcggaataag gagattgagg gtcataggct gttgtttcta aaaagaaacc tgtttcgcct aaacttccat atacttcatc tgtagatacg tggtaaaaaa gatttgtcct tggttcattt gggaatctgc catgcgtatg ttctggattc agtgtccaaa attcacgaca taagttcaat aagttagctg ttccgataac atttgtattg ataaatgcat ttgggtctgt aatacttctg tctacatggc tctctgctgc taaatgtact acagcatccg gattgtattt ttcaaaaacc tttcttaatt cttcaggttt tgtaatatct gctttttcga aaacataatt aggaaaattt tcaatatctt tcaaattttc caggtttcct gcatacgtca atgcatcgag attaataatc gtaatctcag gattttttat cacaaactct cttacaacat gggagccaat aaaacccgcc cctccggtaa tgattatatt cttcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMK NIIITGGAGF IGSHVVREFV IKNPEITIIN LDALTYAGNL ENLKDIENFP NYVFEKADIT KPEELRKVFE KYNPDAVVHL AAESHVDRSI TDPNAFINTN VIGTANLLNL CREFWTLNPE HTHGRFPNEP RTNLFYHVST DEVYGSLGET GFFLETTAYD PQSPYSASKA ASDHLVRAYG NTYGMPFIVS NCSNNYGPNH FPEKLIPLCI SNILNEKPLP IYGDGKYTRD WLYVIDHARA IHQIFNEAKT GETYNIGGFN EWQNIDLVKE LIKQLDAKLG KPEGHSEKLI TFVKDRPGHD KRYAIDATKL NKDLGWKPSV TFEEGLAKTI DWYLDNKEWL ENVTSGDYQK YYENQYS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGA ATATAATCAT TACCGGAGGG GCGGGTTTTA TTGGCTCCCA TGTTGTAAGA GAGTTTGTGA TAAAAAATCC TGAGATTACG ATTATTAATC TCGATGCATT GACGTATGCA GGAAACCTGG AAAATTTGAA AGATATTGAA AATTTTCCTA ATTATGTTTT CGAAAAAGCA GATATTACAA AACCTGAAGA ATTAAGAAAG GTTTTTGAAA AATACAATCC GGATGCTGTA GTACATTTAG CAGCAGAGAG CCATGTAGAC AGAAGTATTA CAGACCCAAA TGCATTTATC AATACAAATG TTATCGGAAC AGCTAACTTA TTGAACTTAT GTCGTGAATT TTGGACACTG AATCCAGAAC ATACGCATGG CAGATTCCCA AATGAACCAA GGACAAATCT TTTTTACCAC GTATCTACAG ATGAAGTATA TGGAAGTTTA GGCGAAACAG GTTTCTTTTT AGAAACAACA GCCTATGACC CTCAATCTCC TTATTCCGCT TCCAAAGCAG CGTCCGATCA TTTGGTAAGA GCTTACGGTA ATACCTATGG AATGCCATTT ATAGTTTCTA ATTGTTCCAA TAATTATGGA CCAAACCACT TCCCTGAAAA GTTGATTCCT TTGTGTATTT CTAATATTTT AAATGAAAAG CCATTGCCAA TATATGGAGA TGGAAAATAT ACAAGAGATT GGTTGTATGT AATTGACCAT GCCAGAGCAA TTCATCAAAT ATTTAATGAA GCTAAAACTG GAGAAACTTA TAATATTGGA GGTTTTAACG AGTGGCAGAA TATTGATCTG GTAAAAGAAT TAATCAAGCA ATTAGATGCT AAATTAGGAA AGCCTGAAGG TCATTCTGAA AAGCTGATTA CATTTGTTAA AGACCGCCCG GGGCATGATA AGCGTTATGC AATTGATGCG ACGAAATTAA ATAAAGATTT AGGCTGGAAA CCCTCCGTTA CTTTTGAAGA AGGATTAGCT AAGACAATTG ATTGGTATTT AGATAATAAA GAGTGGCTAG AGAATGTAAC ATCTGGTGAC TATCAAAAAT ATTATGAAAA TCAATATTCT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for ElanA.00085.a.B1.PS38380
| PURIFICATION DATe: | 11/6/2017 |
| CONCENTRATION: | 21.5mg/ml |
| OBSERVED MW: | 43kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl,5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMK NIIITGGAGF IGSHVVREFV IKNPEITIIN LDALTYAGNL ENLKDIENFP NYVFEKADIT KPEELRKVFE KYNPDAVVHL AAESHVDRSI TDPNAFINTN VIGTANLLNL CREFWTLNPE HTHGRFPNEP RTNLFYHVST DEVYGSLGET GFFLETTAYD PQSPYSASKA ASDHLVRAYG NTYGMPFIVS NCSNNYGPNH FPEKLIPLCI SNILNEKPLP IYGDGKYTRD WLYVIDHARA IHQIFNEAKT GETYNIGGFN EWQNIDLVKE LIKQLDAKLG KPEGHSEKLI TFVKDRPGHD KRYAIDATKL NKDLGWKPSV TFEEGLAKTI DWYLDNKEWL ENVTSGDYQ
Validated NT Sequence
tttttgatag tcaccagatg ttacattctc tagccactct ttattatcta aataccaatc aattgtctta gctaatcctt cttcaaaagt aacggagggt ttccagccta aatctttatt taatttcgtc gcatcaattg cataacgctt atcatgcccc gggcggtctt taacaaatgt aatcagcttt tcagaatgac cttcaggctt tcctaattta gcatctaatt gcttgattaa ttcttttacc agatcaatat tctgccactc gttaaaacct ccaatattat aagtttctcc agttttagct tcattaaata tttgatgaat tgctctggca tggtcaatta catacaacca atctcttgta tattttccat ctccatatat tggcaatggc ttttcattta aaatattaga aatacacaaa ggaatcaact tttcagggaa gtggtttggt ccataattat tggaacaatt agaaactata aatggcattc cataggtatt accgtaagct cttaccaaat gatcggacgc tgctttggaa gcggaataag gagattgagg gtcataggct gttgtttcta aaaagaaacc tgtttcgcct aaacttccat atacttcatc tgtagatacg tggtaaaaaa gatttgtcct tggttcattt gggaatctgc catgcgtatg ttctggattc agtgtccaaa attcacgaca taagttcaat aagttagctg ttccgataac atttgtattg ataaatgcat ttgggtctgt aatacttctg tctacatggc tctctgctgc taaatgtact acagcatccg gattgtattt ttcaaaaacc tttcttaatt cttcaggttt tgtaatatct gctttttcga aaacataatt aggaaaattt tcaatatctt tcaaattttc caggtttcct gcatacgtca atgcatcgag attaataatc gtaatctcag gattttttat cacaaactct cttacaacat gggagccaat aaaacccgcc cctccggtaa tgattatatt cttcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMK NIIITGGAGF IGSHVVREFV IKNPEITIIN LDALTYAGNL ENLKDIENFP NYVFEKADIT KPEELRKVFE KYNPDAVVHL AAESHVDRSI TDPNAFINTN VIGTANLLNL CREFWTLNPE HTHGRFPNEP RTNLFYHVST DEVYGSLGET GFFLETTAYD PQSPYSASKA ASDHLVRAYG NTYGMPFIVS NCSNNYGPNH FPEKLIPLCI SNILNEKPLP IYGDGKYTRD WLYVIDHARA IHQIFNEAKT GETYNIGGFN EWQNIDLVKE LIKQLDAKLG KPEGHSEKLI TFVKDRPGHD KRYAIDATKL NKDLGWKPSV TFEEGLAKTI DWYLDNKEWL ENVTSGDYQK YYENQYS
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGA ATATAATCAT TACCGGAGGG GCGGGTTTTA TTGGCTCCCA TGTTGTAAGA GAGTTTGTGA TAAAAAATCC TGAGATTACG ATTATTAATC TCGATGCATT GACGTATGCA GGAAACCTGG AAAATTTGAA AGATATTGAA AATTTTCCTA ATTATGTTTT CGAAAAAGCA GATATTACAA AACCTGAAGA ATTAAGAAAG GTTTTTGAAA AATACAATCC GGATGCTGTA GTACATTTAG CAGCAGAGAG CCATGTAGAC AGAAGTATTA CAGACCCAAA TGCATTTATC AATACAAATG TTATCGGAAC AGCTAACTTA TTGAACTTAT GTCGTGAATT TTGGACACTG AATCCAGAAC ATACGCATGG CAGATTCCCA AATGAACCAA GGACAAATCT TTTTTACCAC GTATCTACAG ATGAAGTATA TGGAAGTTTA GGCGAAACAG GTTTCTTTTT AGAAACAACA GCCTATGACC CTCAATCTCC TTATTCCGCT TCCAAAGCAG CGTCCGATCA TTTGGTAAGA GCTTACGGTA ATACCTATGG AATGCCATTT ATAGTTTCTA ATTGTTCCAA TAATTATGGA CCAAACCACT TCCCTGAAAA GTTGATTCCT TTGTGTATTT CTAATATTTT AAATGAAAAG CCATTGCCAA TATATGGAGA TGGAAAATAT ACAAGAGATT GGTTGTATGT AATTGACCAT GCCAGAGCAA TTCATCAAAT ATTTAATGAA GCTAAAACTG GAGAAACTTA TAATATTGGA GGTTTTAACG AGTGGCAGAA TATTGATCTG GTAAAAGAAT TAATCAAGCA ATTAGATGCT AAATTAGGAA AGCCTGAAGG TCATTCTGAA AAGCTGATTA CATTTGTTAA AGACCGCCCG GGGCATGATA AGCGTTATGC AATTGATGCG ACGAAATTAA ATAAAGATTT AGGCTGGAAA CCCTCCGTTA CTTTTGAAGA AGGATTAGCT AAGACAATTG ATTGGTATTT AGATAATAAA GAGTGGCTAG AGAATGTAAC ATCTGGTGAC TATCAAAAAT ATTATGAAAA TCAATATTCT TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT