ElanA.00075.a

Porphobilinogen synthase

CENTER ID: ElanA.00075.a
ORGANISM: Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00075.a.B1.GE40569 Full length(ElanA.00075.a) 1 329
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ElanA.00075.a.B1.PW38416 Full length(ElanA.00075.a) 1 329

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1338011.4.peg.1787
UniProt: A0A077EA63

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNFNRNRRLR TSQSVRDLVR ETTLTTNDFV LPIFVMEGSG KKEPIASMPG VFRHSLDLLK EEIKDLYKHG IKAVNVYVKV SDSLKDNTGK EAWNPNGLMQ AAIKLIKDTE PNMIVMPDVA LDPYSVYGHD GIIEKGEVIN DATVDALVRM SLSHAEAGAD FVAPSDMMDG RTFAIRQAFE ESGFTNVGII SYAAKYASAF YGPFRSALDS APVDNQEIPK DKKTYQMDFA NSREAIREVL DDVDEGADII MIKPGMPNLD IVAKVREIIT QPIAVYQVSG EYAMLKAASQ NGWLDNDKVI LESLHSIKRA GADLIFTYFA KEASLLLNK
NT Sequence
ATGAATTTTA ATAGAAACAG AAGACTGCGT ACAAGTCAGT CTGTTAGAGA TTTAGTGCGT GAAACAACAC TAACTACCAA CGATTTTGTA CTTCCAATTT TTGTAATGGA AGGTAGCGGA AAGAAAGAAC CTATAGCTTC TATGCCTGGT GTGTTCAGGC ACTCTTTAGA TCTTTTAAAA GAAGAGATAA AAGATCTTTA TAAGCATGGG ATAAAAGCGG TGAATGTTTA TGTTAAAGTA AGCGACAGCC TGAAAGATAA CACAGGGAAA GAGGCGTGGA ATCCTAATGG GTTAATGCAG GCTGCAATCA AGCTTATTAA AGATACTGAG CCTAATATGA TTGTTATGCC GGATGTTGCC TTGGATCCAT ATTCAGTTTA TGGGCACGAC GGAATTATAG AAAAAGGTGA AGTTATTAAC GATGCTACAG TGGATGCACT AGTAAGAATG AGTCTTTCTC ATGCTGAAGC AGGTGCTGAT TTTGTAGCTC CGTCGGATAT GATGGATGGT CGTACTTTTG CAATACGTCA GGCCTTTGAA GAAAGTGGAT TTACCAATGT AGGTATTATC TCTTATGCGG CTAAATATGC AAGTGCTTTT TACGGACCAT TCAGAAGTGC TTTGGATAGC GCACCGGTAG ATAATCAGGA AATTCCTAAG GACAAGAAAA CTTATCAAAT GGATTTTGCA AACTCTCGTG AGGCTATCCG CGAGGTTTTG GATGATGTGG ATGAAGGTGC TGATATTATT ATGATCAAGC CGGGAATGCC AAATCTGGAT ATTGTAGCAA AAGTAAGAGA GATTATTACT CAGCCAATTG CTGTATATCA GGTGAGCGGA GAATATGCAA TGCTAAAAGC TGCTTCTCAG AACGGATGGT TAGATAATGA CAAAGTTATT CTGGAAAGTT TACACAGCAT TAAGAGAGCG GGAGCAGATT TAATCTTTAC ATATTTTGCT AAAGAAGCTT CATTGCTTTT GAATAAA
Details for ElanA.00075.a.B1.GE40569
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMN FNRNRRLRTS QSVRDLVRET TLTTNDFVLP IFVMEGSGKK EPIASMPGVF RHSLDLLKEE IKDLYKHGIK AVNVYVKVSD SLKDNTGKEA WNPNGLMQAA IKLIKDTEPN MIVMPDVALD PYSVYGHDGI IEKGEVINDA TVDALVRMSL SHAEAGADFV APSDMMDGRT FAIRQAFEES GFTNVGIISY AAKYASAFYG PFRSALDSAP VDNQEIPKDK KTYQMDFANS REAIREVLDD VDEGADIIMI KPGMPNLDIV AKVREIITQP IAVYQVSGEY AMLKAASQNG WLDNDKVILE SLHSIKRAGA DLIFTYFAK
Validated NT Sequence
ttctttagca aaatatgtaa agattaaatc tgctcccgct ctcttaatgc tgtgtaaact ttccagaata actttgtcat tatctaacca tccgttctga gaagcagctt ttagcattgc atattctccg ctcacctgat atacagcaat tggctgagta ataatctctc ttacttttgc tacaatatcc agatttggca ttcccggctt gatcataata atatcagcac cttcatccac atcatccaaa acctcgcgga tagcctcacg agagtttgca aaatccattt gataagtttt cttgtcctta ggaatttcct gattatctac cggtgcgcta tccaaagcac ttctgaatgg tccgtaaaaa gcacttgcat atttagccgc ataagagata atacctacat tggtaaatcc actttcttca aaggcctgac gtattgcaaa agtacgacca tccatcatat ccgacggagc tacaaaatca gcacctgctt cagcatgaga aagactcatt cttactagtg catccactgt agcatcgtta ataacttcac ctttttctat aattccgtcg tgcccataaa ctgaatatgg atccaaggca acatccggca taacaatcat attaggctca gtatctttaa taagcttgat tgcagcctgc attaacccat taggattcca cgcctctttc cctgtgttat ctttcaggct gtcgcttact ttaacataaa cattcaccgc ttttatccca tgcttataaa gatcttttat ctcttctttt aaaagatcta aagagtgcct gaacacacca ggcatagaag ctataggttc tttctttccg ctaccttcca ttacaaaaat tggaagtaca aaatcgttgg tagttagtgt tgtttcacgc actaaatctc taacagactg acttgtacgc agtcttctgt ttctattaaa attcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMN FNRNRRLRTS QSVRDLVRET TLTTNDFVLP IFVMEGSGKK EPIASMPGVF RHSLDLLKEE IKDLYKHGIK AVNVYVKVSD SLKDNTGKEA WNPNGLMQAA IKLIKDTEPN MIVMPDVALD PYSVYGHDGI IEKGEVINDA TVDALVRMSL SHAEAGADFV APSDMMDGRT FAIRQAFEES GFTNVGIISY AAKYASAFYG PFRSALDSAP VDNQEIPKDK KTYQMDFANS REAIREVLDD VDEGADIIMI KPGMPNLDIV AKVREIITQP IAVYQVSGEY AMLKAASQNG WLDNDKVILE SLHSIKRAGA DLIFTYFAKE ASLLLNK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatt ttaatagaaa cagaagactg cgtacaagtc agtctgttag agatttagtg cgtgaaacaa cactaactac caacgatttt gtacttccaa tttttgtaat ggaaggtagc ggaaagaaag aacctatagc ttctatgcct ggtgtgttca ggcactcttt agatctttta aaagaagaga taaaagatct ttataagcat gggataaaag cggtgaatgt ttatgttaaa gtaagcgaca gcctgaaaga taacacaggg aaagaggcgt ggaatcctaa tgggttaatg caggctgcaa tcaagcttat taaagatact gagcctaata tgattgttat gccggatgtt gccttggatc catattcagt ttatgggcac gacggaatta tagaaaaagg tgaagttatt aacgatgcta cagtggatgc actagtaaga atgagtcttt ctcatgctga agcaggtgct gattttgtag ctccgtcgga tatgatggat ggtcgtactt ttgcaatacg tcaggccttt gaagaaagtg gatttaccaa tgtaggtatt atctcttatg cggctaaata tgcaagtgct ttttacggac cattcagaag tgctttggat agcgcaccgg tagataatca ggaaattcct aaggacaaga aaacttatca aatggatttt gcaaactctc gtgaggctat ccgcgaggtt ttggatgatg tggatgaagg tgctgatatt attatgatca agccgggaat gccaaatctg gatattgtag caaaagtaag agagattatt actcagccaa ttgctgtata tcaggtgagc ggagaatatg caatgctaaa agctgcttct cagaacggat ggttagataa tgacaaagtt attctggaaa gtttacacag cattaagaga gcgggagcag atttaatctt tacatatttt gctaaagaag cttcattgct tttgaataaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for ElanA.00075.a.B1.PW38416
PURIFICATION DATe: 1/16/2018
CONCENTRATION: 10.5mg/ml
OBSERVED MW: 40kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
SEC profile is very weak nearly flat
Validated AA Sequence
MAHHHHHHMN FNRNRRLRTS QSVRDLVRET TLTTNDFVLP IFVMEGSGKK EPIASMPGVF RHSLDLLKEE IKDLYKHGIK AVNVYVKVSD SLKDNTGKEA WNPNGLMQAA IKLIKDTEPN MIVMPDVALD PYSVYGHDGI IEKGEVINDA TVDALVRMSL SHAEAGADFV APSDMMDGRT FAIRQAFEES GFTNVGIISY AAKYASAFYG PFRSALDSAP VDNQEIPKDK KTYQMDFANS REAIREVLDD VDEGADIIMI KPGMPNLDIV AKVREIITQP IAVYQVSGEY AMLKAASQNG WLDNDKVILE SLHSIKRAGA DLIFTYFAK
Validated NT Sequence
ttctttagca aaatatgtaa agattaaatc tgctcccgct ctcttaatgc tgtgtaaact ttccagaata actttgtcat tatctaacca tccgttctga gaagcagctt ttagcattgc atattctccg ctcacctgat atacagcaat tggctgagta ataatctctc ttacttttgc tacaatatcc agatttggca ttcccggctt gatcataata atatcagcac cttcatccac atcatccaaa acctcgcgga tagcctcacg agagtttgca aaatccattt gataagtttt cttgtcctta ggaatttcct gattatctac cggtgcgcta tccaaagcac ttctgaatgg tccgtaaaaa gcacttgcat atttagccgc ataagagata atacctacat tggtaaatcc actttcttca aaggcctgac gtattgcaaa agtacgacca tccatcatat ccgacggagc tacaaaatca gcacctgctt cagcatgaga aagactcatt cttactagtg catccactgt agcatcgtta ataacttcac ctttttctat aattccgtcg tgcccataaa ctgaatatgg atccaaggca acatccggca taacaatcat attaggctca gtatctttaa taagcttgat tgcagcctgc attaacccat taggattcca cgcctctttc cctgtgttat ctttcaggct gtcgcttact ttaacataaa cattcaccgc ttttatccca tgcttataaa gatcttttat ctcttctttt aaaagatcta aagagtgcct gaacacacca ggcatagaag ctataggttc tttctttccg ctaccttcca ttacaaaaat tggaagtaca aaatcgttgg tagttagtgt tgtttcacgc actaaatctc taacagactg acttgtacgc agtcttctgt ttctattaaa attcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMN FNRNRRLRTS QSVRDLVRET TLTTNDFVLP IFVMEGSGKK EPIASMPGVF RHSLDLLKEE IKDLYKHGIK AVNVYVKVSD SLKDNTGKEA WNPNGLMQAA IKLIKDTEPN MIVMPDVALD PYSVYGHDGI IEKGEVINDA TVDALVRMSL SHAEAGADFV APSDMMDGRT FAIRQAFEES GFTNVGIISY AAKYASAFYG PFRSALDSAP VDNQEIPKDK KTYQMDFANS REAIREVLDD VDEGADIIMI KPGMPNLDIV AKVREIITQP IAVYQVSGEY AMLKAASQNG WLDNDKVILE SLHSIKRAGA DLIFTYFAKE ASLLLNK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATT TTAATAGAAA CAGAAGACTG CGTACAAGTC AGTCTGTTAG AGATTTAGTG CGTGAAACAA CACTAACTAC CAACGATTTT GTACTTCCAA TTTTTGTAAT GGAAGGTAGC GGAAAGAAAG AACCTATAGC TTCTATGCCT GGTGTGTTCA GGCACTCTTT AGATCTTTTA AAAGAAGAGA TAAAAGATCT TTATAAGCAT GGGATAAAAG CGGTGAATGT TTATGTTAAA GTAAGCGACA GCCTGAAAGA TAACACAGGG AAAGAGGCGT GGAATCCTAA TGGGTTAATG CAGGCTGCAA TCAAGCTTAT TAAAGATACT GAGCCTAATA TGATTGTTAT GCCGGATGTT GCCTTGGATC CATATTCAGT TTATGGGCAC GACGGAATTA TAGAAAAAGG TGAAGTTATT AACGATGCTA CAGTGGATGC ACTAGTAAGA ATGAGTCTTT CTCATGCTGA AGCAGGTGCT GATTTTGTAG CTCCGTCGGA TATGATGGAT GGTCGTACTT TTGCAATACG TCAGGCCTTT GAAGAAAGTG GATTTACCAA TGTAGGTATT ATCTCTTATG CGGCTAAATA TGCAAGTGCT TTTTACGGAC CATTCAGAAG TGCTTTGGAT AGCGCACCGG TAGATAATCA GGAAATTCCT AAGGACAAGA AAACTTATCA AATGGATTTT GCAAACTCTC GTGAGGCTAT CCGCGAGGTT TTGGATGATG TGGATGAAGG TGCTGATATT ATTATGATCA AGCCGGGAAT GCCAAATCTG GATATTGTAG CAAAAGTAAG AGAGATTATT ACTCAGCCAA TTGCTGTATA TCAGGTGAGC GGAGAATATG CAATGCTAAA AGCTGCTTCT CAGAACGGAT GGTTAGATAA TGACAAAGTT ATTCTGGAAA GTTTACACAG CATTAAGAGA GCGGGAGCAG ATTTAATCTT TACATATTTT GCTAAAGAAG CTTCATTGCT TTTGAATAAA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT