SSGCID: Target Page

ElanA.00058.a

Thioredoxin reductase

CENTER ID:	ElanA.00058.a
ORGANISM:	Elizabethkingia anophelis NUHP1
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.00058.a.B1.GE40566	Full length(ElanA.00058.a)		1	316

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
ElanA.00058.a.B1.PW38278	Full length(ElanA.00058.a)		1	316

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1338011.4.peg.2247
UniProt:	A0A077EFC4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MENILDCVIV GSGPAGFTAA IYAARADMKP ELFTGLEPGG QLTTTTEVEN FPGYPSGITG PEMMMDLQKQ AERFETKVHY EMISTVEFAK EAGGIHKLST GSREILAKTV IISTGATAKY LGLDDEKKYS GGGVSACATC DGFFYKGKDV IVVGAGDTAA EEATYLSKIC NKVIMLVRKD HFRASKAMVH RVMNTSNIEV KFNHELVAIE GENNLVERAV AINNITNEKS TIDVHGIFIA IGHKPNTEVF GGQLDLDENG YILTEKGSTR TNLPGVFAAG DVQDHIYRQA ITAAGSGCMA AMDAEKYLSG LEHAEN

NT Sequence

ATGGAAAATA TTTTAGATTG TGTGATTGTA GGCTCTGGGC CTGCAGGATT TACAGCAGCG ATCTACGCTG CAAGAGCAGA CATGAAGCCA GAACTGTTTA CCGGACTTGA ACCGGGTGGG CAATTAACCA CTACAACAGA AGTAGAAAAC TTTCCGGGAT ATCCGTCAGG AATTACAGGG CCTGAAATGA TGATGGATTT GCAAAAACAA GCGGAGCGTT TTGAAACTAA AGTTCACTAT GAGATGATTA GTACTGTAGA ATTTGCTAAA GAAGCAGGTG GCATACACAA GCTAAGCACA GGTAGCCGCG AAATACTGGC TAAAACTGTA ATTATTTCTA CAGGAGCAAC AGCTAAATAT CTTGGATTAG ACGACGAGAA AAAATATAGC GGAGGGGGAG TATCTGCTTG TGCTACATGT GACGGTTTCT TTTATAAAGG TAAAGATGTT ATCGTAGTGG GGGCTGGTGA TACTGCTGCT GAAGAAGCTA CTTATTTGTC CAAAATATGT AATAAGGTAA TAATGCTGGT TCGTAAAGAT CATTTCCGTG CTTCTAAGGC TATGGTTCAT CGTGTAATGA ATACTTCTAA TATTGAAGTG AAATTCAATC ATGAGCTAGT AGCTATTGAA GGAGAAAATA ATCTTGTAGA GAGAGCTGTA GCAATTAATA ACATTACCAA TGAGAAATCA ACAATAGATG TTCATGGTAT TTTCATTGCT ATCGGTCATA AGCCTAATAC TGAAGTTTTT GGTGGTCAGC TTGATTTAGA TGAAAATGGT TATATCCTGA CAGAAAAAGG ATCTACCAGA ACAAACCTTC CTGGTGTATT TGCAGCGGGA GATGTTCAGG ATCACATATA CAGACAGGCA ATCACAGCTG CAGGAAGTGG TTGTATGGCT GCAATGGATG CTGAAAAATA TCTTAGCGGC CTTGAGCATG CTGAGAAT

Details for ElanA.00058.a.B1.GE40566

HARVESTED ON:	10/18/2016
SEQUENCED ON:	10/19/2016
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHHM ENILDCVIVG SGPAGFTAAI YAARADMKPE LFTGLEPGGQ LTTTTEVENF PGYPSGITGP EMMMDLQKQA ERFETKVHYE MISTVEFAKE AGGIHKLSTG SREILAKTVI ISTGATAKYL GLDDEKKYSG GGVSACATCD GFFYKGKDVI VVGAGDTAAE EATYLSKICN KVIMLVRKDH FRASKAMVHR VMNTSNIEVK FNHELVAIEG ENNLVERAVA INNITNEKST IDVHGIFIAI GHKPNTEVFG GQLDLDENGY ILTEKGSTRT NLPGVFAAGD VQDHIYRQAI TAAGSGCMAA MDAEKYLXX

Validated NT Sequence

annnnnnnta agatattttt cagcatccat tgcagccata caaccacttc ctgcagctgt gattgcctgt ctgtatatgt gatcctgaac atctcccgct gcaaatacac caggaaggtt tgttctggta gatccttttt ctgtcaggat ataaccattt tcatctaaat caagctgacc accaaaaact tcagtattag gcttatgacc gatagcaatg aaaataccat gaacatctat tgttgatttc tcattggtaa tgttattaat tgctacagct ctctctacaa gattattttc tccttcaata gctactagct catgattgaa tttcacttca atattagaag tattcattac acgatgaacc atagccttag aagcacggaa atgatcttta cgaaccagca ttattacctt attacatatt ttggacaaat aagtagcttc ttcagcagca gtatcaccag cccccactac gataacatct ttacctttat aaaagaaacc gtcacatgta gcacaagcag atactccccc tccgctatat tttttctcgt cgtctaatcc aagatattta gctgttgctc ctgtagaaat aattacagtt ttagccagta tttcgcggct acctgtgctt agcttgtgta tgccacctgc ttctttagca aattctacag tactaatcat ctcatagtga actttagttt caaaacgctc cgcttgtttt tgcaaatcca tcatcatttc aggccctgta attcctgacg gatatcccgg aaagttttct acttctgttg tagtggttaa ttgcccaccc ggttcaagtc cggtaaacag ttctggcttc atgtctgctc ttgcagcgta gatcgctgct gtaaatcctg caggcccaga gcctacaatc acacaatcta aaatattttc catatggtgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHME NILDCVIVGS GPAGFTAAIY AARADMKPEL FTGLEPGGQL TTTTEVENFP GYPSGITGPE MMMDLQKQAE RFETKVHYEM ISTVEFAKEA GGIHKLSTGS REILAKTVII STGATAKYLG LDDEKKYSGG GVSACATCDG FFYKGKDVIV VGAGDTAAEE ATYLSKICNK VIMLVRKDHF RASKAMVHRV MNTSNIEVKF NHELVAIEGE NNLVERAVAI NNITNEKSTI DVHGIFIAIG HKPNTEVFGG QLDLDENGYI LTEKGSTRTN LPGVFAAGDV QDHIYRQAIT AAGSGCMAAM DAEKYLSGLE HAEN

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa atattttaga ttgtgtgatt gtaggctctg ggcctgcagg atttacagca gcgatctacg ctgcaagagc agacatgaag ccagaactgt ttaccggact tgaaccgggt gggcaattaa ccactacaac agaagtagaa aactttccgg gatatccgtc aggaattaca gggcctgaaa tgatgatgga tttgcaaaaa caagcggagc gttttgaaac taaagttcac tatgagatga ttagtactgt agaatttgct aaagaagcag gtggcataca caagctaagc acaggtagcc gcgaaatact ggctaaaact gtaattattt ctacaggagc aacagctaaa tatcttggat tagacgacga gaaaaaatat agcggagggg gagtatctgc ttgtgctaca tgtgacggtt tcttttataa aggtaaagat gttatcgtag tgggggctgg tgatactgct gctgaagaag ctacttattt gtccaaaata tgtaataagg taataatgct ggttcgtaaa gatcatttcc gtgcttctaa ggctatggtt catcgtgtaa tgaatacttc taatattgaa gtgaaattca atcatgagct agtagctatt gaaggagaaa ataatcttgt agagagagct gtagcaatta ataacattac caatgagaaa tcaacaatag atgttcatgg tattttcatt gctatcggtc ataagcctaa tactgaagtt tttggtggtc agcttgattt agatgaaaat ggttatatcc tgacagaaaa aggatctacc agaacaaacc ttcctggtgt atttgcagcg ggagatgttc aggatcacat atacagacag gcaatcacag ctgcaggaag tggttgtatg gctgcaatgg atgctgaaaa atatcttagc ggccttgagc atgctgagaa ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for ElanA.00058.a.B1.PW38278

PURIFICATION DATe:	5/17/2017
CONCENTRATION:	35mg/ml
OBSERVED MW:	35kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	24
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA ATATTTTAGA TTGTGTGATT GTAGGCTCTG GGCCTGCAGG ATTTACAGCA GCGATCTACG CTGCAAGAGC AGACATGAAG CCAGAACTGT TTACCGGACT TGAACCGGGT GGGCAATTAA CCACTACAAC AGAAGTAGAA AACTTTCCGG GATATCCGTC AGGAATTACA GGGCCTGAAA TGATGATGGA TTTGCAAAAA CAAGCGGAGC GTTTTGAAAC TAAAGTTCAC TATGAGATGA TTAGTACTGT AGAATTTGCT AAAGAAGCAG GTGGCATACA CAAGCTAAGC ACAGGTAGCC GCGAAATACT GGCTAAAACT GTAATTATTT CTACAGGAGC AACAGCTAAA TATCTTGGAT TAGACGACGA GAAAAAATAT AGCGGAGGGG GAGTATCTGC TTGTGCTACA TGTGACGGTT TCTTTTATAA AGGTAAAGAT GTTATCGTAG TGGGGGCTGG TGATACTGCT GCTGAAGAAG CTACTTATTT GTCCAAAATA TGTAATAAGG TAATAATGCT GGTTCGTAAA GATCATTTCC GTGCTTCTAA GGCTATGGTT CATCGTGTAA TGAATACTTC TAATATTGAA GTGAAATTCA ATCATGAGCT AGTAGCTATT GAAGGAGAAA ATAATCTTGT AGAGAGAGCT GTAGCAATTA ATAACATTAC CAATGAGAAA TCAACAATAG ATGTTCATGG TATTTTCATT GCTATCGGTC ATAAGCCTAA TACTGAAGTT TTTGGTGGTC AGCTTGATTT AGATGAAAAT GGTTATATCC TGACAGAAAA AGGATCTACC AGAACAAACC TTCCTGGTGT ATTTGCAGCG GGAGATGTTC AGGATCACAT ATACAGACAG GCAATCACAG CTGCAGGAAG TGGTTGTATG GCTGCAATGG ATGCTGAAAA ATATCTTAGC GGCCTTGAGC ATGCTGAGAA TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT