ElanA.00047.a
Aspartate ammonia-lyase
| CENTER ID: | ElanA.00047.a |
| ORGANISM: | Elizabethkingia anophelis NUHP1 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.00047.a.B1.GE40528 | Full length(ElanA.00047.a) | 1 | 467 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| ElanA.00047.a.B1.PS38143 | Full length(ElanA.00047.a) | 1 | 467 |
Structures
6WNG
DEPOSITED: 4/22/2020
DETERMINATION: XRay
CLONE: ElanA.00047.a.B1.GE40528
PROTEIN: ElanA.00047.a.B1.PS38143
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|1338011.4.peg.2219 |
| UniProt: | A0A077EBQ3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNDYRIESDL IGELKVPVNA YYGVQTQRAI DNFKISNDHL SDHPEFIKAF AFVKKAAAQT NFELGLLDEI INKNIATACD EIIAGKMHKE FPTDMIQGGA GTSMNMNANE VIANRALELM GHQKGEYQFC SPNDHVNLSQ STNDAYPTAI RIALYNLNKT LVERLELLIQ SFRKKADDLK DVIKMGRTQL QDAVPMTMGQ EFNAFANTLQ EEIARLNTNA DLFLETNMGA TAIGTGLNAH PDYAVKCTEN LAKISGADVV LASDLVEATP DTGAYVIYSS AMKRMAVKLS KICNDLRLLA SGPRAGLYEI NLPKMQPGSS IMPGKVNPVI PEVVNQVCFK VIGNDLTVTF AAEAGQLQLN VMEPVLTQSI MESIRFLKNA MDTLREKCID GITANKEICL NMVKNSIGIV TALNPYIGYK NSTKIAKEAL DTGKSVYDLV LEHELLSKEK LDEILAPENM LNPHTKF
NT Sequence
ATGAATGATT ACAGAATTGA AAGTGATCTG ATAGGTGAAC TAAAGGTTCC GGTAAATGCA TACTATGGAG TACAGACGCA GAGAGCGATA GATAATTTTA AAATTTCCAA TGATCACTTA TCGGATCATC CGGAGTTTAT TAAAGCTTTT GCTTTTGTTA AAAAGGCAGC GGCTCAGACT AATTTTGAAT TAGGACTTTT AGATGAGATC ATCAATAAAA ATATAGCAAC GGCCTGTGAC GAGATTATTG CAGGGAAAAT GCATAAAGAG TTTCCAACGG ACATGATCCA AGGTGGAGCA GGAACTTCAA TGAATATGAA TGCGAATGAA GTAATTGCTA ACAGAGCTTT GGAGCTTATG GGGCACCAGA AAGGGGAATA CCAGTTCTGT TCGCCAAACG ATCATGTAAA CCTTTCTCAG TCTACTAACG ATGCGTACCC TACAGCAATT CGTATTGCTT TATACAACCT TAATAAAACA CTTGTAGAGA GGCTGGAATT ACTGATTCAA TCATTCAGAA AGAAAGCAGA TGATCTGAAA GATGTGATCA AAATGGGGCG TACACAGTTG CAGGATGCAG TACCTATGAC AATGGGGCAG GAGTTTAATG CTTTTGCTAA TACCCTTCAG GAAGAAATAG CCCGTTTGAA TACCAATGCA GATTTGTTTC TTGAAACCAA TATGGGAGCG ACGGCAATTG GTACTGGACT TAATGCGCAT CCGGATTATG CTGTAAAATG TACAGAAAAT CTGGCTAAGA TCTCAGGAGC AGATGTAGTA TTGGCTTCAG ACCTTGTAGA GGCAACACCT GATACCGGAG CTTATGTAAT TTATTCTTCC GCAATGAAGA GAATGGCTGT GAAGCTTTCC AAAATTTGTA ATGACTTAAG ATTATTGGCT TCAGGGCCAA GGGCTGGTTT ATATGAAATT AACCTGCCGA AAATGCAGCC AGGATCTTCT ATTATGCCCG GGAAAGTTAA TCCGGTAATT CCGGAAGTGG TCAATCAGGT TTGCTTTAAA GTTATTGGTA ACGATCTTAC AGTAACTTTT GCAGCAGAGG CCGGTCAGTT ACAGCTTAAT GTAATGGAAC CTGTTCTTAC ACAATCCATT ATGGAGTCTA TCCGTTTTCT GAAAAACGCC ATGGATACTC TTCGTGAAAA ATGTATAGAT GGTATTACAG CAAACAAAGA AATTTGCCTG AATATGGTGA AAAATAGTAT TGGTATTGTT ACTGCACTTA ATCCCTATAT TGGTTATAAA AATAGTACGA AGATTGCTAA AGAAGCATTG GACACAGGTA AAAGTGTGTA CGATTTGGTA TTAGAGCATG AGTTATTGAG TAAAGAAAAA CTGGATGAGA TTCTGGCGCC TGAAAATATG CTGAATCCAC ATACAAAATT C
Details for ElanA.00047.a.B1.GE40528
| HARVESTED ON: | 9/1/2016 |
| SEQUENCED ON: | 9/8/2016 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 52kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 94 |
| PERCENT COVERAGE: | 14 |
Validated AA Sequence
MAHHHHHHMN DYRIESDLIG ELKVPVNAYY GVQTQRAIDN FKISNDHLSD HPEFIKAFAF VKKAXXSD
Validated NT Sequence
agatatacca tggctcacca ccaccaccac catatgaatg attacagaat tgaaagtgat ctgataggtg aactaaaggt tccggtaaat gcatactatg gagtacagac gcagagagcg atagataatt ttaaaatttc caatgatcac ttatcggatc atccggagtt tattaaagct tttgcttttg ttaaaaaggc nnnnnnctca gactaatttt gaattaggac ttttagatga gatcatcaat aaaaatatag caacggcctg tgacgagatt attgcaggga aaatgcataa agagtttcca acggacatga tccaaggtgg agcaggaact tcaatgaata tgaatgcgaa tgaagtaatt gctaacagag ctttggagct tatggggcac cagaaagggg aataccagtt ctgttcgcca aacgatcatg taaacctttc tcagtctact aacgatgcgt accctacagc aattcgtatt gctttataca accttaataa aacacttgta gagaggctgg aattactgat tcaatcattc agaaagaaag cagatgatct gaaagatgtg atcaaaatgg ggcgtacaca gttgcaggat gcagtaccta tgacaatggg gcaggagttt aatgcttttg ctaataccct tcaggaagaa atagcccgtt tgaataccaa tgcagatttg tttcttgaaa ccaatatggg agcgacggca attggtactg gacttaatgc gcatccggat tatgctgtaa aatgtacaga aaatctggct aagatctcag gagcagatgt agtattggct tcagaccttg tagaggcaac acctgatacc ggagcttatg taatttattc ttccgcaatg aagagaatgg ctgtgaagct ttccaaaatt tgtaatgact taagattatt ggcttcaggg ccaagggctg gtttatatga aattaacctg ccgaaaatgc agccaggatc ttctattatg cccgggaaag ttaatccggt aattccggaa gtggtcaatc aggtttgctt taaagttatt ggtaacgatc ttacagtaac ttttgcagca gaggccggtc agttacagct taatgtaatg gaacctgttc ttacacaatc cattatggag tctatccgtt ttctgaaaaa cgccatggat actcttcgtg aaaaatgtat agatggtatt acagcaaaca aagaaatttg cctgaatatg gtgaaaaata gtattggtat tgttactgca cttaatccct atattggtta taaaaatagt acgaagattg ctaaagaagc attggacaca ggtaaaagtg tgtacgattt ggtattagag catgagttat tgagtaaaga aaaactggat gagattctgg cgcctgaaaa ta
Expected Protein Sequence
MAHHHHHHMN DYRIESDLIG ELKVPVNAYY GVQTQRAIDN FKISNDHLSD HPEFIKAFAF VKKAAAQTNF ELGLLDEIIN KNIATACDEI IAGKMHKEFP TDMIQGGAGT SMNMNANEVI ANRALELMGH QKGEYQFCSP NDHVNLSQST NDAYPTAIRI ALYNLNKTLV ERLELLIQSF RKKADDLKDV IKMGRTQLQD AVPMTMGQEF NAFANTLQEE IARLNTNADL FLETNMGATA IGTGLNAHPD YAVKCTENLA KISGADVVLA SDLVEATPDT GAYVIYSSAM KRMAVKLSKI CNDLRLLASG PRAGLYEINL PKMQPGSSIM PGKVNPVIPE VVNQVCFKVI GNDLTVTFAA EAGQLQLNVM EPVLTQSIME SIRFLKNAMD TLREKCIDGI TANKEICLNM VKNSIGIVTA LNPYIGYKNS TKIAKEALDT GKSVYDLVLE HELLSKEKLD EILAPENMLN PHTKF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg attacagaat tgaaagtgat ctgataggtg aactaaaggt tccggtaaat gcatactatg gagtacagac gcagagagcg atagataatt ttaaaatttc caatgatcac ttatcggatc atccggagtt tattaaagct tttgcttttg ttaaaaaggc agcggctcag actaattttg aattaggact tttagatgag atcatcaata aaaatatagc aacggcctgt gacgagatta ttgcagggaa aatgcataaa gagtttccaa cggacatgat ccaaggtgga gcaggaactt caatgaatat gaatgcgaat gaagtaattg ctaacagagc tttggagctt atggggcacc agaaagggga ataccagttc tgttcgccaa acgatcatgt aaacctttct cagtctacta acgatgcgta ccctacagca attcgtattg ctttatacaa ccttaataaa acacttgtag agaggctgga attactgatt caatcattca gaaagaaagc agatgatctg aaagatgtga tcaaaatggg gcgtacacag ttgcaggatg cagtacctat gacaatgggg caggagttta atgcttttgc taataccctt caggaagaaa tagcccgttt gaataccaat gcagatttgt ttcttgaaac caatatggga gcgacggcaa ttggtactgg acttaatgcg catccggatt atgctgtaaa atgtacagaa aatctggcta agatctcagg agcagatgta gtattggctt cagaccttgt agaggcaaca cctgataccg gagcttatgt aatttattct tccgcaatga agagaatggc tgtgaagctt tccaaaattt gtaatgactt aagattattg gcttcagggc caagggctgg tttatatgaa attaacctgc cgaaaatgca gccaggatct tctattatgc ccgggaaagt taatccggta attccggaag tggtcaatca ggtttgcttt aaagttattg gtaacgatct tacagtaact tttgcagcag aggccggtca gttacagctt aatgtaatgg aacctgttct tacacaatcc attatggagt ctatccgttt tctgaaaaac gccatggata ctcttcgtga aaaatgtata gatggtatta cagcaaacaa agaaatttgc ctgaatatgg tgaaaaatag tattggtatt gttactgcac ttaatcccta tattggttat aaaaatagta cgaagattgc taaagaagca ttggacacag gtaaaagtgt gtacgatttg gtattagagc atgagttatt gagtaaagaa aaactggatg agattctggc gcctgaaaat atgctgaatc cacatacaaa attctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for ElanA.00047.a.B1.PS38143
| PURIFICATION DATe: | 12/6/2016 |
| CONCENTRATION: | 52.28mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 94 |
| PERCENT COVERAGE: | 14 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN DYRIESDLIG ELKVPVNAYY GVQTQRAIDN FKISNDHLSD HPEFIKAFAF VKKAXXSD
Validated NT Sequence
agatatacca tggctcacca ccaccaccac catatgaatg attacagaat tgaaagtgat ctgataggtg aactaaaggt tccggtaaat gcatactatg gagtacagac gcagagagcg atagataatt ttaaaatttc caatgatcac ttatcggatc atccggagtt tattaaagct tttgcttttg ttaaaaaggc nnnnnnctca gactaatttt gaattaggac ttttagatga gatcatcaat aaaaatatag caacggcctg tgacgagatt attgcaggga aaatgcataa agagtttcca acggacatga tccaaggtgg agcaggaact tcaatgaata tgaatgcgaa tgaagtaatt gctaacagag ctttggagct tatggggcac cagaaagggg aataccagtt ctgttcgcca aacgatcatg taaacctttc tcagtctact aacgatgcgt accctacagc aattcgtatt gctttataca accttaataa aacacttgta gagaggctgg aattactgat tcaatcattc agaaagaaag cagatgatct gaaagatgtg atcaaaatgg ggcgtacaca gttgcaggat gcagtaccta tgacaatggg gcaggagttt aatgcttttg ctaataccct tcaggaagaa atagcccgtt tgaataccaa tgcagatttg tttcttgaaa ccaatatggg agcgacggca attggtactg gacttaatgc gcatccggat tatgctgtaa aatgtacaga aaatctggct aagatctcag gagcagatgt agtattggct tcagaccttg tagaggcaac acctgatacc ggagcttatg taatttattc ttccgcaatg aagagaatgg ctgtgaagct ttccaaaatt tgtaatgact taagattatt ggcttcaggg ccaagggctg gtttatatga aattaacctg ccgaaaatgc agccaggatc ttctattatg cccgggaaag ttaatccggt aattccggaa gtggtcaatc aggtttgctt taaagttatt ggtaacgatc ttacagtaac ttttgcagca gaggccggtc agttacagct taatgtaatg gaacctgttc ttacacaatc cattatggag tctatccgtt ttctgaaaaa cgccatggat actcttcgtg aaaaatgtat agatggtatt acagcaaaca aagaaatttg cctgaatatg gtgaaaaata gtattggtat tgttactgca cttaatccct atattggtta taaaaatagt acgaagattg ctaaagaagc attggacaca ggtaaaagtg tgtacgattt ggtattagag catgagttat tgagtaaaga aaaactggat gagattctgg cgcctgaaaa ta
Expressed Protein Sequence
MAHHHHHHMN DYRIESDLIG ELKVPVNAYY GVQTQRAIDN FKISNDHLSD HPEFIKAFAF VKKAAAQTNF ELGLLDEIIN KNIATACDEI IAGKMHKEFP TDMIQGGAGT SMNMNANEVI ANRALELMGH QKGEYQFCSP NDHVNLSQST NDAYPTAIRI ALYNLNKTLV ERLELLIQSF RKKADDLKDV IKMGRTQLQD AVPMTMGQEF NAFANTLQEE IARLNTNADL FLETNMGATA IGTGLNAHPD YAVKCTENLA KISGADVVLA SDLVEATPDT GAYVIYSSAM KRMAVKLSKI CNDLRLLASG PRAGLYEINL PKMQPGSSIM PGKVNPVIPE VVNQVCFKVI GNDLTVTFAA EAGQLQLNVM EPVLTQSIME SIRFLKNAMD TLREKCIDGI TANKEICLNM VKNSIGIVTA LNPYIGYKNS TKIAKEALDT GKSVYDLVLE HELLSKEKLD EILAPENMLN PHTKF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG ATTACAGAAT TGAAAGTGAT CTGATAGGTG AACTAAAGGT TCCGGTAAAT GCATACTATG GAGTACAGAC GCAGAGAGCG ATAGATAATT TTAAAATTTC CAATGATCAC TTATCGGATC ATCCGGAGTT TATTAAAGCT TTTGCTTTTG TTAAAAAGGC AGCGGCTCAG ACTAATTTTG AATTAGGACT TTTAGATGAG ATCATCAATA AAAATATAGC AACGGCCTGT GACGAGATTA TTGCAGGGAA AATGCATAAA GAGTTTCCAA CGGACATGAT CCAAGGTGGA GCAGGAACTT CAATGAATAT GAATGCGAAT GAAGTAATTG CTAACAGAGC TTTGGAGCTT ATGGGGCACC AGAAAGGGGA ATACCAGTTC TGTTCGCCAA ACGATCATGT AAACCTTTCT CAGTCTACTA ACGATGCGTA CCCTACAGCA ATTCGTATTG CTTTATACAA CCTTAATAAA ACACTTGTAG AGAGGCTGGA ATTACTGATT CAATCATTCA GAAAGAAAGC AGATGATCTG AAAGATGTGA TCAAAATGGG GCGTACACAG TTGCAGGATG CAGTACCTAT GACAATGGGG CAGGAGTTTA ATGCTTTTGC TAATACCCTT CAGGAAGAAA TAGCCCGTTT GAATACCAAT GCAGATTTGT TTCTTGAAAC CAATATGGGA GCGACGGCAA TTGGTACTGG ACTTAATGCG CATCCGGATT ATGCTGTAAA ATGTACAGAA AATCTGGCTA AGATCTCAGG AGCAGATGTA GTATTGGCTT CAGACCTTGT AGAGGCAACA CCTGATACCG GAGCTTATGT AATTTATTCT TCCGCAATGA AGAGAATGGC TGTGAAGCTT TCCAAAATTT GTAATGACTT AAGATTATTG GCTTCAGGGC CAAGGGCTGG TTTATATGAA ATTAACCTGC CGAAAATGCA GCCAGGATCT TCTATTATGC CCGGGAAAGT TAATCCGGTA ATTCCGGAAG TGGTCAATCA GGTTTGCTTT AAAGTTATTG GTAACGATCT TACAGTAACT TTTGCAGCAG AGGCCGGTCA GTTACAGCTT AATGTAATGG AACCTGTTCT TACACAATCC ATTATGGAGT CTATCCGTTT TCTGAAAAAC GCCATGGATA CTCTTCGTGA AAAATGTATA GATGGTATTA CAGCAAACAA AGAAATTTGC CTGAATATGG TGAAAAATAG TATTGGTATT GTTACTGCAC TTAATCCCTA TATTGGTTAT AAAAATAGTA CGAAGATTGC TAAAGAAGCA TTGGACACAG GTAAAAGTGT GTACGATTTG GTATTAGAGC ATGAGTTATT GAGTAAAGAA AAACTGGATG AGATTCTGGC GCCTGAAAAT ATGCTGAATC CACATACAAA ATTCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT