EhruA.01330.a

Thymidylate synthase

CENTER ID: EhruA.01330.a
ORGANISM: Ehrlichia ruminantium Gardel
ASSOCIATED DISEASE: Heartwater
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhruA.01330.a.D11.GE00001 EhruA.01330.a 1 297
EhruA.01330.a.D12.GE00001 EhruA.01330.a 1 248
EhruA.01330.a.D13.GE00001 EhruA.01330.a 1 254
EhruA.01330.a.D14.GE00001 EhruA.01330.a 32 297
EhruA.01330.a.D15.GE00001 EhruA.01330.a 32 248
EhruA.01330.a.D16.GE00001 EhruA.01330.a 32 254
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_155488
PATRIC ID: fig|302409.5.peg.750
RefSeq: YP_196632.1
UniProt: Q5FG35
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLVLSIRIKV WQTMESEITK RIVVPELEDI LYKEHKVLDK GFVRLVDYMG SDESVVQAAR ISYGRGTKSV SQDAALINYL MRHSHTTPFE MCEIKFHIKL PIFVARQWVR HRTANVNEYS ARYSVLDHEF YIPELDHVAT QSEDNAQGRG NSLSNEDAQY VTDLLKRDSD MVYETYNKFL IKGVSREISR ISLTLNYYTE WYWKIDLHNL LHFLRLRSDV HAQYEIRVYA ETMLEIVKKW VPLTYAAFVE YCLESQSFSK SALSVVKKLI AGEDVAREDT GIGKREWREL MDVLADK
NT Sequence
ttgttagtat taagtattag aattaaagta tggcagacta tggaatcaga gattacaaaa cgtatagttg ttcctgaatt agaagatatt ctttataagg aacataaggt tttagataag gggttcgtca ggttagtaga ttatatggga tctgatgaat ctgtagtaca ggctgctaga atatcctatg gaagaggtac taaaagtgta agccaggatg ctgcgttgat taattatctt atgcggcatt cacatactac accatttgaa atgtgtgaaa taaaatttca tattaaatta cctatatttg ttgcaagaca gtgggtacgt catagaactg caaatgttaa tgaatattct gctagatatt ctgttttaga tcatgaattt tatattcctg agcttgatca tgtggcaact caatctgaag ataatgcaca aggtagaggt aatagcttgt ctaatgagga tgctcagtat gtaactgatc tattaaaaag agattctgat atggtatatg aaacctataa taagttttta ataaaaggtg tttcaagaga aatatcacgt attagcctta ctctaaatta ttacactgaa tggtattgga aaatagatct acataattta ttgcattttt taagattgag gtctgatgtg catgctcaat atgaaattag agtctatgct gagactatgt tggaaattgt aaaaaagtgg gtgccattaa cttatgcagc ttttgttgaa tattgtttag aatcacaaag tttttctaag agtgcattga gtgttgtcaa gaagctaatt gctggtgagg atgttgctag agaagatact ggtattggta aaagagagtg gagagagttg atggatgttt tagcagataa ataa
Details for EhruA.01330.a.D11.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/28/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 79
Validated AA Sequence
MEDNDIIEAH XEQIGXMLVX SXXIKVWQTM ESEITKRIVV PELEDXLYKE HKVLDKGFVR LVDYMGSDES VVQAARISYG RGTKSVSQDA ALINYXMRHS HTTPFEMCEI KFHIKLPIFV ARQWVRHRTA NVNEYSARYS VLDHEFYIPE LDHVATQSED NAQGRGNSLS NEDAQYVTDL LKRDSDMVYE TYNKFLIKGV SREISRISLT LNYYTEWYWK IDLHNLLHFL RLRSDVHAQY EIRVYAETML EIVKKWVPLT YAAFVEYCLE SQSFSKSALS VVKKLIAGED VAREDTGIGK REWRELMDVL ADK
Validated NT Sequence
ttatttatcg gccagtacat ccatcagttc gcgccattca cgtttaccga tgccagtgtc ctcacgagca acgtcttcgc cagcgatcag tttcttaaca accgaaagag cagatttaga gaaggactgg gattcgaggc aatactcaac gaatgcggcg taagtcagtg gcacccattt cttaactatt tcgagcatgg tttcggcgta tacacggatt tcgtactgtg cgtgaacatc agaacggaga cgcaggaagt gcagcaagtt gtgcaggtcg atcttccagt accattcagt gtaatagttc agggtcaggc tgatacgaga gatctcgcgg gaaacgccct tgatcaggaa cttgttgtag gtttcgtaga ccatgtcaga atcacgtttc agcaggtcgg tcacgtattg tgcgtcttcg ttggacagag aattaccgcg gccctgggca ttgtcctcag actgagtagc tacgtggtcc agttctggga tatagaattc gtgatcgagg acagagtagc gagcggagta ctcgttgacg ttagcggtac gatgacgtac ccactggcga gctacaaaga ttggcagttt gatatggaac ttgatttcgc acatctcgaa cggagtggta tgcgagtggc gcatcangta gttaatcaga gctgcatcct gagaaacaga cttggtgccg cggccgtagg agatacgagc tgcctgaact acgctttcgt cggaacccat gtagtcaacc agacgcacga aacccttatc cagcactttg tgttccttat agaggangtc ttccagttcc ggtacaacta tacgtttggt aatttcggat tccatggtct gccaaacttt gatgcngang ctcngnacta acattncacc gatttgttca cngtgtgctt cgataatgtc gttatcttcc atgtncagan c
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMLVL SIRIKVWQTM ESEITKRIVV PELEDILYKE HKVLDKGFVR LVDYMGSDES VVQAARISYG RGTKSVSQDA ALINYLMRHS HTTPFEMCEI KFHIKLPIFV ARQWVRHRTA NVNEYSARYS VLDHEFYIPE LDHVATQSED NAQGRGNSLS NEDAQYVTDL LKRDSDMVYE TYNKFLIKGV SREISRISLT LNYYTEWYWK IDLHNLLHFL RLRSDVHAQY EIRVYAETML EIVKKWVPLT YAAFVEYCLE SQSFSKSALS VVKKLIAGED VAREDTGIGK REWRELMDVL ADK
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gttagtcctg agcatccgca tcaaagtttg gcagaccatg gaatccgaaa ttaccaaacg tatagttgta ccggaactgg aagacatcct ctataaggaa cacaaagtgc tggataaggg tttcgtgcgt ctggttgact acatgggttc cgacgaaagc gtagttcagg cagctcgtat ctcctacggc cgcggcacca agtctgtttc tcaggatgca gctctgatta actacctgat gcgccactcg cataccactc cgttcgagat gtgcgaaatc aagttccata tcaaactgcc aatctttgta gctcgccagt gggtacgtca tcgtaccgct aacgtcaacg agtactccgc tcgctactct gtcctcgatc acgaattcta tatcccagaa ctggaccacg tagctactca gtctgaggac aatgcccagg gccgcggtaa ttctctgtcc aacgaagacg cacaatacgt gaccgacctg ctgaaacgtg attctgacat ggtctacgaa acctacaaca agttcctgat caagggcgtt tcccgcgaga tctctcgtat cagcctgacc ctgaactatt acactgaatg gtactggaag atcgacctgc acaacttgct gcacttcctg cgtctccgtt ctgatgttca cgcacagtac gaaatccgtg tatacgccga aaccatgctc gaaatagtta agaaatgggt gccactgact tacgccgcat tcgttgagta ttgcctcgaa tcccagtcct tctctaaatc tgctctttcg gttgttaaga aactgatcgc tggcgaagac gttgctcgtg aggacactgg catcggtaaa cgtgaatggc gcgaactgat ggatgtactg gccgataaat aaaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for EhruA.01330.a.D12.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/28/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 86
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG MLVLSIRIKV WQTMESEITK RIVVPELEDI LYKEHKVLDK GFVRLVDYMG SDESVVQAAR ISYGRGTKSV SQDAALINYL MRHSHTTPFE MCEIKFHIKL PIFVARQWVR HRTANVNEYS ARYSVLDHEF YIPELDHVAT QSEDNAQGRG NSLSNEDAQY VTDLLKRDSD MVYETYNKFL IKGVSREISR ISLTLNYYTE WYWKIDLHNL LHFLRLRSDV HAQYEIRVYA ETMLEIVKKW VPLTYAAF
Validated NT Sequence
ttagaatgcg gcgtaagtca gtggcaccca tttcttaact atttcgagca tggtttcggc gtatacacgg atttcgtact gtgcgtgaac atcagaacgg agacgcagga agtgcagcaa gttgtgcagg tcgatcttcc agtaccattc agtgtaatag ttcagggtca ggctgatacg agagatctcg cgggaaacgc ccttgatcag gaacttgttg taggtttcgt agaccatgtc agaatcacgt ttcagcaggt cggtcacgta ttgtgcgtct tcgttggaca gagaattacc gcggccctgg gcattgtcct cagactgagt agctacgtgg tccagttctg ggatatagaa ttcgtgatcg aggacagagt agcgagcgga gtactcgttg acgttagcgg tacgatgacg tacccactgg cgagctacaa agattggcag tttgatatgg aacttgattt cgcacatctc gaacggagtg gtatgcgagt ggcgcatcag gtagttaatc agagctgcat cctgagaaac agacttggtg ccgcggccgt aggagatacg agctgcctga actacgcttt cgtcggaacc catgtagtca accagacgca cgaaaccctt atccagcact ttgtgttcct tatagaggat gtcttccagt tccggtacaa ctatacgttt ggtaatttcg gattccatgg tctgccaaac tttgatgcgg atgctcagga ctaacattcc accgatttgt tcacggtgtg cttcgataat gtcgttatct tccatgtcca gatcttccgg agtctggtct gcctgaatac ggataccgtc gtacagaaag cgcagggaat ccatttcctt gccctggcgt ttagcgaatg cttccatcag gcgacgcagc ggggtggtct tcttaatctt gaagaagatt tcgctgctac cgtcgctaac cttcaggttg atgtgggtct ccggcttcac ttccggtttt acttcgccg
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMLVL SIRIKVWQTM ESEITKRIVV PELEDILYKE HKVLDKGFVR LVDYMGSDES VVQAARISYG RGTKSVSQDA ALINYLMRHS HTTPFEMCEI KFHIKLPIFV ARQWVRHRTA NVNEYSARYS VLDHEFYIPE LDHVATQSED NAQGRGNSLS NEDAQYVTDL LKRDSDMVYE TYNKFLIKGV SREISRISLT LNYYTEWYWK IDLHNLLHFL RLRSDVHAQY EIRVYAETML EIVKKWVPLT YAAF
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gttagtcctg agcatccgca tcaaagtttg gcagaccatg gaatccgaaa ttaccaaacg tatagttgta ccggaactgg aagacatcct ctataaggaa cacaaagtgc tggataaggg tttcgtgcgt ctggttgact acatgggttc cgacgaaagc gtagttcagg cagctcgtat ctcctacggc cgcggcacca agtctgtttc tcaggatgca gctctgatta actacctgat gcgccactcg cataccactc cgttcgagat gtgcgaaatc aagttccata tcaaactgcc aatctttgta gctcgccagt gggtacgtca tcgtaccgct aacgtcaacg agtactccgc tcgctactct gtcctcgatc acgaattcta tatcccagaa ctggaccacg tagctactca gtctgaggac aatgcccagg gccgcggtaa ttctctgtcc aacgaagacg cacaatacgt gaccgacctg ctgaaacgtg attctgacat ggtctacgaa acctacaaca agttcctgat caagggcgtt tcccgcgaga tctctcgtat cagcctgacc ctgaactatt acactgaatg gtactggaag atcgacctgc acaacttgct gcacttcctg cgtctccgtt ctgatgttca cgcacagtac gaaatccgtg tatacgccga aaccatgctc gaaatagtta agaaatgggt gccactgact tacgccgcat tctaaaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gat
Details for EhruA.01330.a.D13.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/28/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMLVL SIRIKVWQTM ESEITKRIVV PELEDILYKE HKVLDKGFVR LVDYMGSDES VVQAARISYG RGTKSVSQDA ALINYLMRHS HTTPFEMCEI KFHIKLPIFV ARQWVRHRTA NVNEYSARYS VLDHEFYIPE LDHVATQSED NAQGRGNSLS NEDAQYVTDL LKRDSDMVYE TYNKFLIKGV SREISRISLT LNYYTEWYWK IDLHNLLHFL RLRSDVHAQY EIRVYAETML EIVKKWVPLT YAAFVEYCLE
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gttagtcctg agcatccgca tcaaagtttg gcagaccatg gaatccgaaa ttaccaaacg tatagttgta ccggaactgg aagacatcct ctataaggaa cacaaagtgc tggataaggg tttcgtgcgt ctggttgact acatgggttc cgacgaaagc gtagttcagg cagctcgtat ctcctacggc cgcggcacca agtctgtttc tcaggatgca gctctgatta actacctgat gcgccactcg cataccactc cgttcgagat gtgcgaaatc aagttccata tcaaactgcc aatctttgta gctcgccagt gggtacgtca tcgtaccgct aacgtcaacg agtactccgc tcgctactct gtcctcgatc acgaattcta tatcccagaa ctggaccacg tagctactca gtctgaggac aatgcccagg gccgcggtaa ttctctgtcc aacgaagacg cacaatacgt gaccgacctg ctgaaacgtg attctgacat ggtctacgaa acctacaaca agttcctgat caagggcgtt tcccgcgaga tctctcgtat cagcctgacc ctgaactatt acactgaatg gtactggaag atcgacctgc acaacttgct gcacttcctg cgtctccgtt ctgatgttca cgcacagtac gaaatccgtg tatacgccga aaccatgctc gaaatagtta agaaatgggt gccactgact tacgccgcat tcgttgagta ttgcctcgaa taa
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGMLVL SIRIKVWQTM ESEITKRIVV PELEDILYKE HKVLDKGFVR LVDYMGSDES VVQAARISYG RGTKSVSQDA ALINYLMRHS HTTPFEMCEI KFHIKLPIFV ARQWVRHRTA NVNEYSARYS VLDHEFYIPE LDHVATQSED NAQGRGNSLS NEDAQYVTDL LKRDSDMVYE TYNKFLIKGV SREISRISLT LNYYTEWYWK IDLHNLLHFL RLRSDVHAQY EIRVYAETML EIVKKWVPLT YAAFVEYCLE
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggaat gttagtcctg agcatccgca tcaaagtttg gcagaccatg gaatccgaaa ttaccaaacg tatagttgta ccggaactgg aagacatcct ctataaggaa cacaaagtgc tggataaggg tttcgtgcgt ctggttgact acatgggttc cgacgaaagc gtagttcagg cagctcgtat ctcctacggc cgcggcacca agtctgtttc tcaggatgca gctctgatta actacctgat gcgccactcg cataccactc cgttcgagat gtgcgaaatc aagttccata tcaaactgcc aatctttgta gctcgccagt gggtacgtca tcgtaccgct aacgtcaacg agtactccgc tcgctactct gtcctcgatc acgaattcta tatcccagaa ctggaccacg tagctactca gtctgaggac aatgcccagg gccgcggtaa ttctctgtcc aacgaagacg cacaatacgt gaccgacctg ctgaaacgtg attctgacat ggtctacgaa acctacaaca agttcctgat caagggcgtt tcccgcgaga tctctcgtat cagcctgacc ctgaactatt acactgaatg gtactggaag atcgacctgc acaacttgct gcacttcctg cgtctccgtt ctgatgttca cgcacagtac gaaatccgtg tatacgccga aaccatgctc gaaatagtta agaaatgggt gccactgact tacgccgcat tcgttgagta ttgcctcgaa taaaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for EhruA.01330.a.D14.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/28/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 87
Validated AA Sequence
MEAFAKRQGK EMDSLRFLYD GIRIQADQTP EDLDMEDNDI IEAHREQIGG YKEHKVLDKG FVRLVDYMGS DESVVQAARI SYGRGTKSVS QDAALINYLM RHSHTTPFEM CEIKFHIKLP IFVARQWVRH RTANVNEYSA RYSVLDHEFY IPELDHVATQ SEDNAQGRGN SLSNEDAQYV TDLLKRDSDM VYETYNKFLI KGVSREISRI SLTLNYYTEW YWKIDLHNLL HFLRLRSDVH AQYEIRVYAE TMLEIVKKWV PLTYAAFVEY CLESQSFSKS ALSVVKKLIA GEDVAREDTG IGKREWRELM DVLADK
Validated NT Sequence
ccaccaccac caccaccata gcggcgaagt aaaaccggaa gtgaagccgg agacccacat caacctgaag gttagcgacg gtagcagcga aatcttcttc aagattaaga agaccacccc gctgcgtcgc ctgatggaag cattcgctaa acgccagggc aaggaaatgg attccctgcg ctttctgtac gacggtatcc gtattcaggc agaccagact ccggaagatc tggacatgga agataacgac attatcgaag cacaccgtga acaaatcggt ggatataagg aacacaaagt gctggataag ggtttcgtgc gtctggttga ctacatgggt tccgacgaaa gcgtagttca ggcagctcgt atctcctacg gccgcggcac caagtctgtt tctcaggatg cagctctgat taactacctg atgcgccact cgcataccac tccgttcgag atgtgcgaaa tcaagttcca tatcaaactg ccaatctttg tagctcgcca gtgggtacgt catcgtaccg ctaacgtcaa cgagtactcc gctcgctact ctgtcctcga tcacgaattc tatatcccag aactggacca cgtagctact cagtctgagg acaatgccca gggccgcggt aattctctgt ccaacgaaga cgcacaatac gtgaccgacc tgctgaaacg tgattctgac atggtctacg aaacctacaa caagttcctg atcaagggcg tttcccgcga gatctctcgt atcagcctga ccctgaacta ttacactgaa tggtactgga agatcgacct gcacaacttg ctgcacttcc tgcgtctccg ttctgatgtt cacgcacagt acgaaatccg tgtatacgcc gaaaccatgc tcgaaatagt taagaaatgg gtgccactga cttacgccgc attcgttgag tattgcctcg aatcccagtc cttctctaaa tctgctcttt cggttgttaa gaaactgatc gctggcgaag acgttgctcg tgaggacact ggcatcggta aacgtgaatg gcgcgaactg atggatgtac tggccgataa ataa
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYKEH KVLDKGFVRL VDYMGSDESV VQAARISYGR GTKSVSQDAA LINYLMRHSH TTPFEMCEIK FHIKLPIFVA RQWVRHRTAN VNEYSARYSV LDHEFYIPEL DHVATQSEDN AQGRGNSLSN EDAQYVTDLL KRDSDMVYET YNKFLIKGVS REISRISLTL NYYTEWYWKI DLHNLLHFLR LRSDVHAQYE IRVYAETMLE IVKKWVPLTY AAFVEYCLES QSFSKSALSV VKKLIAGEDV AREDTGIGKR EWRELMDVLA DK
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata taaggaacac aaagtgctgg ataagggttt cgtgcgtctg gttgactaca tgggttccga cgaaagcgta gttcaggcag ctcgtatctc ctacggccgc ggcaccaagt ctgtttctca ggatgcagct ctgattaact acctgatgcg ccactcgcat accactccgt tcgagatgtg cgaaatcaag ttccatatca aactgccaat ctttgtagct cgccagtggg tacgtcatcg taccgctaac gtcaacgagt actccgctcg ctactctgtc ctcgatcacg aattctatat cccagaactg gaccacgtag ctactcagtc tgaggacaat gcccagggcc gcggtaattc tctgtccaac gaagacgcac aatacgtgac cgacctgctg aaacgtgatt ctgacatggt ctacgaaacc tacaacaagt tcctgatcaa gggcgtttcc cgcgagatct ctcgtatcag cctgaccctg aactattaca ctgaatggta ctggaagatc gacctgcaca acttgctgca cttcctgcgt ctccgttctg atgttcacgc acagtacgaa atccgtgtat acgccgaaac catgctcgaa atagttaaga aatgggtgcc actgacttac gccgcattcg ttgagtattg cctcgaatcc cagtccttct ctaaatctgc tctttcggtt gttaagaaac tgatcgctgg cgaagacgtt gctcgtgagg acactggcat cggtaaacgt gaatggcgcg aactgatgga tgtactggcc gataaataaa agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat
Details for EhruA.01330.a.D15.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/31/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYKEH KVLDKGFVRL VDYMGSDESV VQAARISYGR GTKSVSQDAA LINYLMRHSH TTPFEMCEIK FHIKLPIFVA RQWVRHRTAN VNEYSARYSV LDHEFYIPEL DHVATQSEDN AQGRGNSLSN EDAQYVTDLL KRDSDMVYET YNKFLIKGVS REISRISLTL NYYTEWYWKI DLHNLLHFLR LRSDVHAQYE IRVYAETMLE IVKKWVPLTY AAF
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata taaggaacac aaagtgctgg ataagggttt cgtgcgtctg gttgactaca tgggttccga cgaaagcgta gttcaggcag ctcgtatctc ctacggccgc ggcaccaagt ctgtttctca ggatgcagct ctgattaact acctgatgcg ccactcgcat accactccgt tcgagatgtg cgaaatcaag ttccatatca aactgccaat ctttgtagct cgccagtggg tacgtcatcg taccgctaac gtcaacgagt actccgctcg ctactctgtc ctcgatcacg aattctatat cccagaactg gaccacgtag ctactcagtc tgaggacaat gcccagggcc gcggtaattc tctgtccaac gaagacgcac aatacgtgac cgacctgctg aaacgtgatt ctgacatggt ctacgaaacc tacaacaagt tcctgatcaa gggcgtttcc cgcgagatct ctcgtatcag cctgaccctg aactattaca ctgaatggta ctggaagatc gacctgcaca acttgctgca cttcctgcgt ctccgttctg atgttcacgc acagtacgaa atccgtgtat acgccgaaac catgctcgaa atagttaaga aatgggtgcc actgacttac gccgcattct aa
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYKEH KVLDKGFVRL VDYMGSDESV VQAARISYGR GTKSVSQDAA LINYLMRHSH TTPFEMCEIK FHIKLPIFVA RQWVRHRTAN VNEYSARYSV LDHEFYIPEL DHVATQSEDN AQGRGNSLSN EDAQYVTDLL KRDSDMVYET YNKFLIKGVS REISRISLTL NYYTEWYWKI DLHNLLHFLR LRSDVHAQYE IRVYAETMLE IVKKWVPLTY AAF
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata taaggaacac aaagtgctgg ataagggttt cgtgcgtctg gttgactaca tgggttccga cgaaagcgta gttcaggcag ctcgtatctc ctacggccgc ggcaccaagt ctgtttctca ggatgcagct ctgattaact acctgatgcg ccactcgcat accactccgt tcgagatgtg cgaaatcaag ttccatatca aactgccaat ctttgtagct cgccagtggg tacgtcatcg taccgctaac gtcaacgagt actccgctcg ctactctgtc ctcgatcacg aattctatat cccagaactg gaccacgtag ctactcagtc tgaggacaat gcccagggcc gcggtaattc tctgtccaac gaagacgcac aatacgtgac cgacctgctg aaacgtgatt ctgacatggt ctacgaaacc tacaacaagt tcctgatcaa gggcgtttcc cgcgagatct ctcgtatcag cctgaccctg aactattaca ctgaatggta ctggaagatc gacctgcaca acttgctgca cttcctgcgt ctccgttctg atgttcacgc acagtacgaa atccgtgtat acgccgaaac catgctcgaa atagttaaga aatgggtgcc actgacttac gccgcattct aaaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
Details for EhruA.01330.a.D16.GE00001
HARVESTED ON: data unavailable
SEQUENCED ON: 7/31/2009
EXPECTED MW: 0kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: kanamycin
COUNT OF EXPRESSION COLONIES: data unavailable
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYKEH KVLDKGFVRL VDYMGSDESV VQAARISYGR GTKSVSQDAA LINYLMRHSH TTPFEMCEIK FHIKLPIFVA RQWVRHRTAN VNEYSARYSV LDHEFYIPEL DHVATQSEDN AQGRGNSLSN EDAQYVTDLL KRDSDMVYET YNKFLIKGVS REISRISLTL NYYTEWYWKI DLHNLLHFLR LRSDVHAQYE IRVYAETMLE IVKKWVPLTY AAFVEYCLE
Validated NT Sequence
atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata taaggaacac aaagtgctgg ataagggttt cgtgcgtctg gttgactaca tgggttccga cgaaagcgta gttcaggcag ctcgtatctc ctacggccgc ggcaccaagt ctgtttctca ggatgcagct ctgattaact acctgatgcg ccactcgcat accactccgt tcgagatgtg cgaaatcaag ttccatatca aactgccaat ctttgtagct cgccagtggg tacgtcatcg taccgctaac gtcaacgagt actccgctcg ctactctgtc ctcgatcacg aattctatat cccagaactg gaccacgtag ctactcagtc tgaggacaat gcccagggcc gcggtaattc tctgtccaac gaagacgcac aatacgtgac cgacctgctg aaacgtgatt ctgacatggt ctacgaaacc tacaacaagt tcctgatcaa gggcgtttcc cgcgagatct ctcgtatcag cctgaccctg aactattaca ctgaatggta ctggaagatc gacctgcaca acttgctgca cttcctgcgt ctccgttctg atgttcacgc acagtacgaa atccgtgtat acgccgaaac catgctcgaa atagttaaga aatgggtgcc actgacttac gccgcattcg ttgagtattg cctcgaataa
Expected Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGYKEH KVLDKGFVRL VDYMGSDESV VQAARISYGR GTKSVSQDAA LINYLMRHSH TTPFEMCEIK FHIKLPIFVA RQWVRHRTAN VNEYSARYSV LDHEFYIPEL DHVATQSEDN AQGRGNSLSN EDAQYVTDLL KRDSDMVYET YNKFLIKGVS REISRISLTL NYYTEWYWKI DLHNLLHFLR LRSDVHAQYE IRVYAETMLE IVKKWVPLTY AAFVEYCLE
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggccacc accaccacca ccatagcggc gaagtaaaac cggaagtgaa gccggagacc cacatcaacc tgaaggttag cgacggtagc agcgaaatct tcttcaagat taagaagacc accccgctgc gtcgcctgat ggaagcattc gctaaacgcc agggcaagga aatggattcc ctgcgctttc tgtacgacgg tatccgtatt caggcagacc agactccgga agatctggac atggaagata acgacattat cgaagcacac cgtgaacaaa tcggtggata taaggaacac aaagtgctgg ataagggttt cgtgcgtctg gttgactaca tgggttccga cgaaagcgta gttcaggcag ctcgtatctc ctacggccgc ggcaccaagt ctgtttctca ggatgcagct ctgattaact acctgatgcg ccactcgcat accactccgt tcgagatgtg cgaaatcaag ttccatatca aactgccaat ctttgtagct cgccagtggg tacgtcatcg taccgctaac gtcaacgagt actccgctcg ctactctgtc ctcgatcacg aattctatat cccagaactg gaccacgtag ctactcagtc tgaggacaat gcccagggcc gcggtaattc tctgtccaac gaagacgcac aatacgtgac cgacctgctg aaacgtgatt ctgacatggt ctacgaaacc tacaacaagt tcctgatcaa gggcgtttcc cgcgagatct ctcgtatcag cctgaccctg aactattaca ctgaatggta ctggaagatc gacctgcaca acttgctgca cttcctgcgt ctccgttctg atgttcacgc acagtacgaa atccgtgtat acgccgaaac catgctcgaa atagttaaga aatgggtgcc actgacttac gccgcattcg ttgagtattg cctcgaataa aagcttgcgg ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat