EhchA.17987.a

DNA polymerase III subunit beta (EC 2.7.7.7)

CENTER ID: EhchA.17987.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.17987.a.B11.GE41340 full length 1 369
EhchA.17987.a.B12.GE41814 full length 1 369
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.871
UniProt: Q2GFI8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNFFVSKDKL LQALSYINSI VEKRHSIVVL SCVRIEARDQ EIKLTSTDLD ISIIASITAH VNVNGVVVVS AQLLYDIVKK LPNDIEIKFF IGEDGRLTIK CGRTEFFLPV VDIEKFPIIE NNELQNKFTL QVSELVSLFG KTKFAISIEE SRFNLNGLYV HTSNNILCCA ATDGHRLSLT KLSQSQNVLS NFGIIVPKKT VNEILKIVSH GSGNDLISIS LSDRKICFEY KEYILISKVI DGTFPDYNSI IPRSHDKNVL IESETLSNAI DRVSVVVFDK VKVIKLRFVS NKLILSAASS DQGDAREELE VDYSGEDMTI GLNARYLMEA LYCIKGKCLL SFYDAGTSVL IQEEENIDDF TYIIMPMRI
NT Sequence
ttgaactttt ttgttagcaa agataaatta ctgcaagctt tgtcttacat taatagtatt gtagaaaaac gtcatagtat tgttgtattg tcgtgtgtaa gaatagaagc aagggatcaa gaaattaagt taacatctac agatcttgat atttcaataa ttgcatctat tactgcccat gtaaacgtta atggagttgt agtagtgtct gctcaacttc tttatgatat tgtaaaaaag ttgcctaatg atattgaaat caaatttttt attggtgaag atggaagatt aactataaaa tgtggtcgta cggagttttt tcttcctgta gttgatatag agaagtttcc tattattgaa aataatgaat tacaaaacaa atttactttg caagtctctg aattggttag tttgtttggt aaaactaaat ttgcaatttc catagaagag agtagattta atttgaatgg attatatgtt catacgtcca ataatatctt atgttgtgct gctacagatg gacataggtt gtcgttaact aagttgagtc aatctcagaa tgtattgtca aattttggta ttatagttcc caaaaaaaca gttaatgaaa ttttgaagat agtaagtcat ggtagtggta atgatttaat atcaattagt ttatcagata ggaagatttg ttttgagtat aaagaatata ttttaatatc taaagtaata gatggtactt ttccggatta taattctatt attcctagat ctcatgataa gaatgtatta attgaatctg aaacgttatc taatgcgata gatagagtat ctgtagtagt ctttgataaa gtaaaagtga taaaattaag gtttgtgagt aataaactta tattaagtgc tgcttcttca gatcaaggtg atgctagaga ggaattagaa gtagattatt ctggagaaga catgactatt ggtttaaatg ccagatattt aatggaagct ttgtattgta taaaaggaaa atgtctttta tcgttttatg atgctggtac atctgtttta atacaggaag aagaaaatat agatgatttc acttacatta ttatgcctat gagaatatag
Details for EhchA.17987.a.B11.GE41340
HARVESTED ON: 4/3/2017
SEQUENCED ON: 4/10/2017
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML NFFVSKDKLL QALSYINSIV EKRHSIVVLS CVRIEARDQE IKLTSTDLDI SIIASITAHV NVNGVVVVSA QLLYDIVKKL PNDIEIKFFI GEDGRLTIKC GRTEFFLPVV DIEKFPIIEN NELQNKFTLQ VSELVSLFGK TKFAISIEES RFNLNGLYVH TSNNILCCAA TDGHRLSLTK LSQSQNVLSN FGIIVPKKTV NEILKIVSHG SGNDLISISL SDRKICFEYK EYILISKVID GTFPDYNSII PRSHDKNVLI ESETLSNAID RVSVVVFDKV KVIKLRFVSN KLILSAASSD QGDAREELEV DYSGEDMTIG LNARYLMEAL YCIKGKCLLS FYDAGTSVLI QEEENIDDFT
Validated NT Sequence
gtaagtgaaa tcatctatat tttcttcttc ctgtattaaa acagatgtac cagcatcata aaacgataaa agacattttc cttttataca atacaaagct tccattaaat atctggcatt taaaccaata gtcatgtctt ctccagaata atctacttct aattcctctc tagcatcacc ttgatctgaa gaagcagcac ttaatataag tttattactc acaaacctta attttatcac ttttacttta tcaaagacta ctacagatac tctatctatc gcattagata acgtttcaga ttcaattaat acattcttat catgagatct aggaataata gaattataat ccggaaaagt accatctatt actttagata ttaaaatata ttctttatac tcaaaacaaa tcttcctatc tgataaacta attgatatta aatcattacc actaccatga cttactatct tcaaaatttc attaactgtt tttttgggaa ctataatacc aaaatttgac aatacattct gagattgact caacttagtt aacgacaacc tatgtccatc tgtagcagca caacataaga tattattgga cgtatgaaca tataatccat tcaaattaaa tctactctct tctatggaaa ttgcaaattt agttttacca aacaaactaa ccaattcaga gacttgcaaa gtaaatttgt tttgtaattc attattttca ataataggaa acttctctat atcaactaca ggaagaaaaa actccgtacg accacatttt atagttaatc ttccatcttc accaataaaa aatttgattt caatatcatt aggcaacttt tttacaatat cataaagaag ttgagcagac actactacaa ctccattaac gtttacatgg gcagtaatag atgcaattat tgaaatatca agatctgtag atgttaactt aatttcttga tcccttgctt ctattcttac acacgacaat acaacaatac tatgacgttt ttctacaata ctattaatgt aagacaaagc ttgcagtaat ttatctttgc taacaaaaaa gttcaacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMN FFVSKDKLLQ ALSYINSIVE KRHSIVVLSC VRIEARDQEI KLTSTDLDIS IIASITAHVN VNGVVVVSAQ LLYDIVKKLP NDIEIKFFIG EDGRLTIKCG RTEFFLPVVD IEKFPIIENN ELQNKFTLQV SELVSLFGKT KFAISIEESR FNLNGLYVHT SNNILCCAAT DGHRLSLTKL SQSQNVLSNF GIIVPKKTVN EILKIVSHGS GNDLISISLS DRKICFEYKE YILISKVIDG TFPDYNSIIP RSHDKNVLIE SETLSNAIDR VSVVVFDKVK VIKLRFVSNK LILSAASSDQ GDAREELEVD YSGEDMTIGL NARYLMEALY CIKGKCLLSF YDAGTSVLIQ EEENIDDFTY IIMPMRI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaact tttttgttag caaagataaa ttactgcaag ctttgtctta cattaatagt attgtagaaa aacgtcatag tattgttgta ttgtcgtgtg taagaataga agcaagggat caagaaatta agttaacatc tacagatctt gatatttcaa taattgcatc tattactgcc catgtaaacg ttaatggagt tgtagtagtg tctgctcaac ttctttatga tattgtaaaa aagttgccta atgatattga aatcaaattt tttattggtg aagatggaag attaactata aaatgtggtc gtacggagtt ttttcttcct gtagttgata tagagaagtt tcctattatt gaaaataatg aattacaaaa caaatttact ttgcaagtct ctgaattggt tagtttgttt ggtaaaacta aatttgcaat ttccatagaa gagagtagat ttaatttgaa tggattatat gttcatacgt ccaataatat cttatgttgt gctgctacag atggacatag gttgtcgtta actaagttga gtcaatctca gaatgtattg tcaaattttg gtattatagt tcccaaaaaa acagttaatg aaattttgaa gatagtaagt catggtagtg gtaatgattt aatatcaatt agtttatcag ataggaagat ttgttttgag tataaagaat atattttaat atctaaagta atagatggta cttttccgga ttataattct attattccta gatctcatga taagaatgta ttaattgaat ctgaaacgtt atctaatgcg atagatagag tatctgtagt agtctttgat aaagtaaaag tgataaaatt aaggtttgtg agtaataaac ttatattaag tgctgcttct tcagatcaag gtgatgctag agaggaatta gaagtagatt attctggaga agacatgact attggtttaa atgccagata tttaatggaa gctttgtatt gtataaaagg aaaatgtctt ttatcgtttt atgatgctgg tacatctgtt ttaatacagg aagaagaaaa tatagatgat ttcacttaca ttattatgcc tatgagaata tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for EhchA.17987.a.B12.GE41814
HARVESTED ON: 8/7/2017
SEQUENCED ON: 9/20/2017
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHML NFFVSKDKLL QALSYINSIV EKRHSIVVLS CVRIEARDQE IKLTSTDLDI SIIASITAHV NVNGVVVVSA QLLYDIVKKL PNDIEIKFFI GEDGRLTIKC GRTEFFLPVV DIEKFPIIEN NELQNKFTLQ VSELVSLFGK TKFAISIEES RFNLNGLYVH TSNNILCCAA TDGHRLSLTK LSQSQNVLSN FGIIVPKKTV NEILKIVSHG SGNDLISISL SDRKICFEYK EYILISKVID GTFPDYNSII PRSHDKNVLI ESETLSNAID RVSVVVFDKV KVIKLRFVSN KLILSAASSD QGDAREELEV DYSGEDMTIG LNARYLMEAL YCIKGKCLLS FYDAGTSVLI QEEENIDD
Validated NT Sequence
gaaatcatct atattttctt cttcctgtat taaaacagat gtaccagcat cataaaacga taaaagacat tttcctttta tacaatacaa agcttccatt aaatatctgg catttaaacc aatagtcatg tcttctccag aataatctac ttctaattcc tctctagcat caccttgatc tgaagaagca gcacttaata taagtttatt actcacaaac cttaatttta tcacttttac tttatcaaag actactacag atactctatc tatcgcatta gataacgttt cagattcaat taatacattc ttatcatgag atctaggaat aatagaatta taatccggaa aagtaccatc tattacttta gatattaaaa tatattcttt atactcaaaa caaatcttcc tatctgataa actaattgat attaaatcat taccactacc atgacttact atcttcaaaa tttcattaac tgtttttttg ggaactataa taccaaaatt tgacaataca ttctgagatt gactcaactt agttaacgac aacctatgtc catctgtagc agcacaacat aagatattat tggacgtatg aacatataat ccattcaaat taaatctact ctcttctatg gaaattgcaa atttagtttt accaaacaaa ctaaccaatt cagagacttg caaagtaaat ttgttttgta attcattatt ttcaataata ggaaacttct ctatatcaac tacaggaaga aaaaactccg tacgaccaca ttttatagtt aatcttccat cttcaccaat aaaaaatttg atttcaatat cattaggcaa cttttttaca atatcataaa gaagttgagc agacactact acaactccat taacgtttac atgggcagta atagatgcaa ttattgaaat atcaagatct gtagatgtta acttaatttc ttgatccctt gcttctattc ttacacacga caatacaaca atactatgac gtttttctac aatactatta atgtaagaca aagcttgcag taatttatct ttgctaacaa aaaagttcaa catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMN FFVSKDKLLQ ALSYINSIVE KRHSIVVLSC VRIEARDQEI KLTSTDLDIS IIASITAHVN VNGVVVVSAQ LLYDIVKKLP NDIEIKFFIG EDGRLTIKCG RTEFFLPVVD IEKFPIIENN ELQNKFTLQV SELVSLFGKT KFAISIEESR FNLNGLYVHT SNNILCCAAT DGHRLSLTKL SQSQNVLSNF GIIVPKKTVN EILKIVSHGS GNDLISISLS DRKICFEYKE YILISKVIDG TFPDYNSIIP RSHDKNVLIE SETLSNAIDR VSVVVFDKVK VIKLRFVSNK LILSAASSDQ GDAREELEVD YSGEDMTIGL NARYLMEALY CIKGKCLLSF YDAGTSVLIQ EEENIDDFTY IIMPMRI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaact tttttgttag caaagataaa ttactgcaag ctttgtctta cattaatagt attgtagaaa aacgtcatag tattgttgta ttgtcgtgtg taagaataga agcaagggat caagaaatta agttaacatc tacagatctt gatatttcaa taattgcatc tattactgcc catgtaaacg ttaatggagt tgtagtagtg tctgctcaac ttctttatga tattgtaaaa aagttgccta atgatattga aatcaaattt tttattggtg aagatggaag attaactata aaatgtggtc gtacggagtt ttttcttcct gtagttgata tagagaagtt tcctattatt gaaaataatg aattacaaaa caaatttact ttgcaagtct ctgaattggt tagtttgttt ggtaaaacta aatttgcaat ttccatagaa gagagtagat ttaatttgaa tggattatat gttcatacgt ccaataatat cttatgttgt gctgctacag atggacatag gttgtcgtta actaagttga gtcaatctca gaatgtattg tcaaattttg gtattatagt tcccaaaaaa acagttaatg aaattttgaa gatagtaagt catggtagtg gtaatgattt aatatcaatt agtttatcag ataggaagat ttgttttgag tataaagaat atattttaat atctaaagta atagatggta cttttccgga ttataattct attattccta gatctcatga taagaatgta ttaattgaat ctgaaacgtt atctaatgcg atagatagag tatctgtagt agtctttgat aaagtaaaag tgataaaatt aaggtttgtg agtaataaac ttatattaag tgctgcttct tcagatcaag gtgatgctag agaggaatta gaagtagatt attctggaga agacatgact attggtttaa atgccagata tttaatggaa gctttgtatt gtataaaagg aaaatgtctt ttatcgtttt atgatgctgg tacatctgtt ttaatacagg aagaagaaaa tatagatgat ttcacttaca ttattatgcc tatgagaata tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat