SSGCID: Target Page

EhchA.17844.a

Ankyrin repeat protein (Ank200)

CENTER ID:	EhchA.17844.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.17844.a.A2.GE32216	N-acidic domain major epitope-containing region		1	398

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.597
RefSeq:	YP_507490.1
UniProt:	Q2GGE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADPKQDNLQ QNPNSQEDNI QSSDQVNPQP GGEDQQHVEG QPPLAQPVEE GAVGGLDVAD LPSQQETGET VEEGLYAVPL PKDQRPTPTQ VLEEDPSVEE EEIAPPLPPR GDVAELQEAV EEDPLYAVPL PKGQRPAPTQ VLEEDPSVEE EEEIAPPLPP RNNVGEVEPQ EDPIYQGIPG HQEEMEEDPY ASLDQVSQGA GADGIQENPV PQEAGEELEE DIYQDPADFQ GLGQGGQQLD QAGYQGPSIG DRQLVNGPYG FNDGSYAMEF DDVMWEGVRD AVIHDEEIDP KFLVTDGLMR HICDKIVQSE GNLPEPDLEE IVSILKNDKE GISELINEPV QVDIPNNPVR EGRNVMTLLH LAYAYNVDPR IINAIESVEN SFGESGLDGY NIQDADGNLP LHHAAKNCNG QVLDNCISKT NSNIINIRNF GNQSPLHVMV QNPGCSIGNI QVANECGMDF NLIDHPTGRM PIHYAAEAAS SEVLSYVIRN TKAESPQASA VNTQDVNGRT PLHCAAISGN SKGLSVMLLQ NGVDCAVRDK NYSTPLHYAV AGNDIKSIKN LCSVKGRVQG VKSSAASLLC EDLQGDTPLH IACKVEGTKA FETVRQSIKK HHGKQVLQEL LIREGSGPRL NVSGFGSQSI LSGVSGDLYG YLNSQNFPTS PVHAAVKANN LQLLNLFLKK SPDILRQSSP NGFNPVHMAA LFADVKTVKL IIENASGEEV NAQSDSTLTP LHLACIRGDG SIIKRMVEHE SVNVNQTMGP DQNTVLQYAI NRGNHSLIKR LLSHPSIDLN VRNADGKTSA HSAMEKGDLK TVKALCNAGA DVNTVDNNGR SVISSAIYSG QNEKKLVPIV KLLLNSGAKI GQSEDKNILL QKCINSGYNK LLDLLLEQGE RINVEGKASP LVSAVVSGNT HAVKKLVASG GDINQKVSDE NSIHYKNSLL MIAVENTDLV MLELLSSKGC NATAVGRDGN TACHMLVNHQ EKGFAEKGVK TLISKGGRTA RDILSKQNSN GDTPLHLALK LSGTKIASAM ISALDKKDFS KIAGAKNDAG ETLLHVAVNS GNPDLVKLLV SNLNKDVLEA LACTPNNNGD TPLHCALKNY DGKCARIILE GCDKRDLSAV IGVQNLYGNT ALHLALRPGA EGLQTIQQGL NEVVDLMLNG LDKESLSSTV NVRNDEGKTP VHYAVSGDST YSKKLIKACS CDTLTIPSLD GKLLSGCVEG ESHLKKPKTI LDKVLGRSVY KTLLKYEAKA AVHGLHGPDF SSPKSLQEGI QSGKFQGVSS SSESLSEKEI QSEQVQGSSP FQEFIPSVPV YKTLDGVEEG VDSSKSQKSV LHRLPSYSPE LNTITMSPTV SLGASAKESG FETDTVSVSS SVEEISSLSS SGLSSLSSRG LSSSSESLFL SGIFDKGEGQ HKASEEQLQE LSEEITDIVQ GLPPITSEDI GAQAVSPSTS QGADVKKSSC QSK

NT Sequence

atggcagatc caaaacaaga taatttacaa caaaatccta attcgcaaga ggataatatt caaagttcag atcaggttaa tcctcagcct ggtggagaag atcaacaaca tgttgaaggt caaccaccgc tagcccaacc agtagaagaa ggtgctgttg gtggattaga tgttgctgat ttgccttctc agcaagaaac aggagaaact gtagaagaag gtttgtatgc ggtaccttta cctaaagatc agagacctac tcctactcaa gtattagaag aagatccttc tgttgaagaa gaagaaattg ctcctccact tccgccaaga ggggatgtag ctgagcttca ggaagcagtt gaagaagatc cattgtatgc ggtaccttta cctaaaggtc aaagacctgc tcctactcaa gtattagaag aagatccttc tgttgaagaa gaggaagaaa ttgctcctcc acttccgcca agaaacaatg tgggtgaagt tgagcctcag gaagatccta tatatcaggg aatacccggg catcaggaag aaatggaaga agatccatat gcgagtcttg atcaagtttc acaaggtgca ggtgcagatg gaattcagga aaaccctgta cctcaagaag caggtgaaga acttgaagaa gatatatatc aagatcctgc tgacttccaa ggacttggtc aaggtggtca acagttagat caagcgggat atcaaggtcc tagtataggg gacagacagt tagtgaatgg accatatggc tttaatgatg gttcgtacgc tatggagttt gacgatgtta tgtgggaagg tgttagggat gcagttattc atgatgaaga gatagatcct aaatttctag taactgatgg gttaatgcgt catatttgtg ataaaatagt acaatctgaa ggaaatttac ctgagccaga tctagaagaa attgttagta ttttaaaaaa tgataaggaa gggatatctg aactaattaa tgagccagtg caagttgata ttcctaataa tcctgtccgt gaaggtagaa atgtaatgac attactgcat ttagcttatg catataatgt tgatccaagg atcattaacg ctatagaaag tgtagagaat tcattcggcg agagtggtct tgatggttat aatattcagg atgctgatgg aaatttacca ttgcaccatg ctgctaaaaa ttgtaatggt caagtattgg ataattgtat atctaagaca aatagtaata taattaatat tagaaatttt ggaaatcaat ccccattgca tgttatggtt cagaaccctg gctgttctat agggaacata caggttgcta atgaatgtgg tatggatttt aatcttatag atcatcctac tggtaggatg cctatacatt atgctgctga agctgcttct tctgaggtgc tatcatatgt aatcagaaat acaaaagctg agtctcctca agctagtgca gttaatacac aagatgtaaa tggacgtact ccattgcatt gtgcggcaat cagcggtaat tcaaaagggt tatctgtgat gttattacaa aatggtgtag attgtgctgt acgtgataag aattatagta cacctttaca ttatgctgtt gctggtaatg atataaaatc gataaaaaat ttatgttcag ttaaaggacg tgttcaaggt gttaaaagtt ctgctgcatc attattatgt gaagatttgc aaggtgatac acctttgcat attgcttgta aagttgaagg taccaaggca tttgaaaccg taaggcaatc tataaaaaag caccatggga aacaagtttt gcaagaatta ttaataagag aaggaagtgg gcctaggtta aatgtgtctg gttttggttc gcagagtata ttatcaggtg taagtggaga cttatatggt tatctgaatt cacaaaattt tcctacttct ccagtgcatg ctgctgttaa agcgaataat ctacaattgc ttaatttatt tttaaaaaaa tctccagata tcctcagaca atcgtcccct aatggattca atcctgttca tatggctgct ttatttgcag atgttaaaac tgtaaaattg attattgaga acgcttcagg agaagaagtt aatgctcaga gtgatagtac acttactcca ttacatctag catgtatccg aggtgatggt agtataataa aaaggatggt tgaacatgaa agtgtgaatg ttaatcaaac tatgggaccc gatcaaaata ctgtgttgca atatgcaatt aatagaggta accatagtct tattaagaga ttattgtccc atccttctat tgatcttaat gttagaaatg ctgatggtaa aacgtctgcg cattctgcga tggaaaaagg agatttgaaa actgtaaaag ctctatgtaa tgctggagca gatgttaata ctgtggataa taacggccgt tctgtgatat ctagtgctat atattcagga caaaatgaaa aaaaacttgt tcctatagtt aagcttttat taaatagtgg tgcaaaaatt ggtcaaagtg aggataaaaa tatactttta caaaagtgta taaattctgg gtataacaaa ttattagatt tattactgga acaaggtgaa aggattaatg tagaaggtaa agcatctcca ttagtttctg ctgtagtgtc tggtaatact catgctgtta agaaactggt tgctagtggt ggagatatta atcaaaaagt ctctgatgag aacagtattc attataaaaa tagtttgtta atgattgctg tggaaaacac tgatcttgtt atgctggaat tgttatcttc taaaggatgt aatgctactg ccgtaggaag agatggtaat acagcatgtc atatgcttgt taaccatcaa gaaaaaggtt ttgctgaaaa gggtgtaaaa acgctaatat ctaaaggtgg aagaactgcc agggatattc tctcaaaaca aaatagtaat ggtgatacac cgctgcattt agctcttaag ttatctggta ctaagatagc atctgctatg atatctgctt tggacaaaaa agatttctca aagattgcgg gagcaaaaaa tgatgcagga gagacgttat tacatgtagc tgttaattca ggtaatccag atttagtaaa attattagta agtaatttaa ataaagatgt gctagaggct ttagcatgta caccaaataa caatggtgat acacctttgc attgtgcact gaagaattat gatggtaaat gtgcgcgaat aatattagaa ggttgtgata aaagggattt aagtgctgta ataggtgtgc aaaatttgta cggtaataca gcattgcatt tagctctgag acctggtgct gaaggcttac agaccattca gcaaggtttg aatgaagtag ttgatttaat gttaaatggt ttggataaag aatctttgtc aagtacagtt aatgttcgaa atgacgaagg gaaaactcct gtgcattatg ctgtatcagg ggatagtaca tattccaaaa aattgattaa agcatgtagt tgcgacacat taacgatacc tagtttggat ggtaaattat tatcaggatg tgttgaaggg gaaagtcatc ttaaaaagcc taaaacaata ttagataagg tcttaggacg ttctgtatac aagaccctac taaaatatga agcaaaagct gcagttcatg gtctacatgg acccgatttt agtagtccta aatcattaca agaggggata caaagtggaa agtttcaggg agtctctagt agtagtgaat cattatcaga aaaggaaata cagagtgaac aggttcaggg atcatctccg tttcaggagt ttattcctag tgttcctgtt tataaaacat tagatggtgt agaagaaggt gttgatagca gtaagtcgca aaaatctgtg ttgcatagac ttccatctta ttctcctgaa ctgaatacaa taacaatgag tcctactgta tctctaggtg catcagcgaa ggagagtggt tttgagaccg ataccgtatc tgtatcttcc tctgtggagg aaatatcgag tttaagcagc agtggtttat catcgttatc ttctagagga ttgtcatctt caagtgaatc attatttctt agtggaatat ttgataaggg tgaaggacaa cataaggctt cagaagaaca gctacaagaa ttaagtgagg aaattacaga tattgttcag ggactacctc ctattacatc tgaagatata ggtgctcaag cagtatctcc tagtacaagt caaggtgctg atgtgaaaaa aagttcttgc cagagcaaat aa

Details for EhchA.17844.a.A2.GE32216

HARVESTED ON:	7/28/2011
SEQUENCED ON:	8/3/2011
EXPECTED MW:	45kDa
OBSERVED MW:	68kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	85

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMADPKQDNL QQNPNSQEDN IQSSDQVNPQ PGGEDQQHVE GQPPLAQPVE EGAVGGLDVA DLPSQQETGE TVEEGLYAVP LPKDQRPTPT QVLEEDPSVE EEEIAPPLPP RGDVAELQEA VEEDPLYAVP LPKGQRPAPT QVLEEDPSVE EEEEIAPPLP PRNNVGEVEP QEDPIYQGIP GHQEEMEEDP YASLDQVSQG AGADGIQENP VPQEAGEELE EDIYQDPADF QGLGQGGQQL DQAGYQGPSI GDRQLVNGPY GFNDGSYAME FDDVMWEGVR DAVIHDEEID PKFLVTDGLM RHICDKIVQS EGNLPEPDLE EIVSILKNDK EGISELIN

Validated NT Sequence

tcattaatta gttcagatat cccttcctta tcatttttta aaatactaac aatttcttct agatctggct caggtaaatt tccttcagat tgtactattt tatcacaaat atgacgcatt aacccatcag ttactagaaa tttaggatct atctcttcat catgaataac tgcatcccta acaccttccc acataacatc gtcaaactcc atagcgtacg aaccatcatt aaagccatat ggtccattca ctaactgtct gtcccctata ctaggacctt gatatcccgc ttgatctaac tgttgaccac cttgaccaag tccttggaag tcagcaggat cttgatatat atcttcttca agttcttcac ctgcttcttg aggtacaggg ttttcctgaa ttccatctgc acctgcacct tgtgaaactt gatcaagact cgcatatgga tcttcttcca tttcttcctg atgcccgggt attccctgat atataggatc ttcctgaggc tcaacttcac ccacattgtt tcttggcgga agtggaggag caatttcttc ctcttcttca acagaaggat cttcttctaa tacttgagta ggagcaggtc tttgaccttt aggtaaaggt accgcataca atggatcttc ttcaactgct tcctgaagct cagctacatc ccctcttggc ggaagtggag gagcaatttc ttcttcttca acagaaggat cttcttctaa tacttgagta ggagtaggtc tctgatcttt aggtaaaggt accgcataca aaccttcttc tacagtttct cctgtttctt gctgagaagg caaatcagca acatctaatc caccaacagc accttcttct actggttggg ctagcggtgg ttgaccttca acatgttgtt gatcttctcc accaggctga ggattaacct gatctgaact ttgaatatta tcctcttgcg aattaggatt ttgttgtaaa ttatcttgtt ttggatctgc catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagatcca aaacaagata atttacaaca aaatcctaat tcgcaagagg ataatattca aagttcagat caggttaatc ctcagcctgg tggagaagat caacaacatg ttgaaggtca accaccgcta gcccaaccag tagaagaagg tgctgttggt ggattagatg ttgctgattt gccttctcag caagaaacag gagaaactgt agaagaaggt ttgtatgcgg tacctttacc taaagatcag agacctactc ctactcaagt attagaagaa gatccttctg ttgaagaaga agaaattgct cctccacttc cgccaagagg ggatgtagct gagcttcagg aagcagttga agaagatcca ttgtatgcgg tacctttacc taaaggtcaa agacctgctc ctactcaagt attagaagaa gatccttctg ttgaagaaga ggaagaaatt gctcctccac ttccgccaag aaacaatgtg ggtgaagttg agcctcagga agatcctata tatcagggaa tacccgggca tcaggaagaa atggaagaag atccatatgc gagtcttgat caagtttcac aaggtgcagg tgcagatgga attcaggaaa accctgtacc tcaagaagca ggtgaagaac ttgaagaaga tatatatcaa gatcctgctg acttccaagg acttggtcaa ggtggtcaac agttagatca agcgggatat caaggtccta gtatagggga cagacagtta gtgaatggac catatggctt taatgatggt tcgtacgcta tggagtttga cgatgttatg tgggaaggtg ttagggatgc agttattcat gatgaagaga tagatcctaa atttctagta actgatgggt taatgcgtca tatttgtgat aaaatagtac aatctgaagg aaatttacct gagccagatc tagaagaaat tgttagtatt ttaaaaaatg ataaggaagg gatatctgaa ctaattaatg agccagtgca agttgatatt cctaataatc ctgtccgtga aggtagaaat gtaatgacat tactgcattt agcttatgca tataatgttg atccaaggat cattaacgct atagaaagtg tagagaattc attcggcgag agtggtcttg atggttataa tattcaggat gctgatggaa attaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa