EhchA.17688.a

Putative uncharacterized protein

CENTER ID: EhchA.17688.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.17688.a.A2.GE30853 Mature protein( EhchA.17688.a ) 26 302
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.389
RefSeq: YP_507272.1
UniProt: Q2GH11

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKIFYRLLY VCLTIVAITL VGKALTILYT KTTLNQHTNL SNKPYKINVN HSLTHIIGYD LLRPIIESRI DYYIKINGLV DYLEHIKHKT IKPDLFKIHD IVEGNGKKAL CGQNISAIIH KFLYDSESKN ILSQALKNIP QEVTFKLGES IIKELNYTIA GMEEEGERII ILTDQEGNLK EQYYVKLISI NSSYPDEVND ILIFNNTIEN YEGTVNKIHC GDSVSVKYTV RESNGKVLLE DQELKVEIGK NEAPLAIELG LINMRSDMTR SMIVSPKLFT NFEKPENFDE NSIKIIDMNI IE
NT Sequence
ATGAAGAAAA TATTTTATAG ATTATTATAT GTCTGTCTTA CCATAGTAGC TATAACACTT GTAGGAAAAG CATTAACAAT ATTATACACA AAAACAACAC TCAACCAACA TACAAATCTT TCTAATAAAC CATACAAAAT TAATGTTAAT CATTCTCTGA CACACATTAT AGGATATGAT TTATTAAGAC CAATCATTGA ATCTCGTATT GATTACTACA TAAAAATAAA TGGATTAGTT GATTACCTTG AGCACATTAA ACATAAAACT ATAAAACCAG ATCTCTTTAA AATACACGAT ATTGTAGAAG GAAATGGTAA AAAAGCACTA TGTGGACAAA ATATTTCCGC AATAATACAT AAATTCCTAT ATGATTCAGA AAGCAAAAAC ATCCTTTCTC AAGCATTAAA AAATATTCCA CAGGAAGTAA CATTTAAATT AGGAGAAAGT ATTATAAAAG AACTAAATTA TACAATAGCA GGTATGGAAG AAGAAGGTGA ACGTATTATT ATACTAACAG ACCAAGAAGG CAACTTAAAA GAACAATACT ATGTTAAATT AATATCCATT AATTCATCAT ATCCAGATGA AGTCAATGAT ATATTAATTT TTAACAATAC TATTGAAAAT TATGAAGGTA CTGTCAATAA GATACACTGT GGAGATAGTG TAAGCGTAAA ATATACAGTA CGAGAATCAA ATGGAAAAGT ATTACTAGAA GATCAAGAAT TAAAAGTAGA AATTGGCAAA AATGAAGCTC CTCTTGCGAT AGAGTTAGGA CTCATTAATA TGAGATCAGA TATGACACGA TCCATGATTG TATCTCCTAA ACTTTTTACT AATTTCGAAA AACCTGAAAA TTTTGATGAA AATAGTATTA AAATAATTGA TATGAACATT ATTGAA
Details for EhchA.17688.a.A2.GE30853
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTILYTKTT LNQHTNLSNK PYKINVNHSL THIIGYDLLR PIIESRIDYY IKINGLVDYL EHIKHKTIKP DLFKIHDIVE GNGKKALCGQ NISAIIHKFL YDSESKNILS QALKNIPQEV TFKLGESIIK ELNYTIAGME EEGERIIILT DQEGNLKEQY YVKLISINSS YPDEVNDILI FNNTIENYEG TVNKIHCGDS VSVXYTVRES NGKVLLEDQE LKVEIGKNEA PLAIELGLIN MRXDMTRSMI VSPKLFTNFE KPENFDENSI KIIDXNIIE
Validated NT Sequence
tttgtttact ttaagnngga gntatacnat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgacaata ttatacacaa aaacaacact caaccaacat acaaatcttt ctaataaacc atacaaaatt aatgttaatc attctctgac acacattata ggatatgatt tattaagacc aatcattgaa tctcgtattg attactacat aaaaataaat ggattagttg attaccttga gcacattaaa cataaaacta taaaaccaga tctctttaaa atacacgata ttgtagaagg aaatggtaaa aaagcactat gtggacaaaa tatttccgca ataatacata aattcctata tgattcagaa agcaaaaaca tcctttctca agcattaaaa aatattccac aggaagtaac atttaaatta ggagaaagta ttataaaaga actaaattat acaatagcag gtatggaaga agaaggtgaa cgtattatta tactaacaga ccaagaaggc aacttaaaag aacaatacta tgttaaatta atatccatta attcatcata tccagatgaa gtcaatgata tattaatttt taacaatact attgaaaatt atgaaggtac tgtcaataag atacactgtg gagatagtgt aagcgtanaa tatacagtac gagaatcaaa tggaaaagta ttactagaag atcaagaatt aaaagtagaa attggcaaaa atgaagctcc tcttgcgata gagttaggac tcattaatat gagancagat atgacacgat ccatgattgt atctcctaaa ctttttacta atttcgaaaa acctgaaaat tttgatgaaa atagtattaa aataattgat angaacatta ttgaataata aacagcacga acaagttctg cagcnngctn ctcnnnnnnn nnctgctaac naagccngna ngnagctnan tnntnntnnn nnnnnnnnnn nnnnnnnaan aaaana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTILYTKTT LNQHTNLSNK PYKINVNHSL THIIGYDLLR PIIESRIDYY IKINGLVDYL EHIKHKTIKP DLFKIHDIVE GNGKKALCGQ NISAIIHKFL YDSESKNILS QALKNIPQEV TFKLGESIIK ELNYTIAGME EEGERIIILT DQEGNLKEQY YVKLISINSS YPDEVNDILI FNNTIENYEG TVNKIHCGDS VSVKYTVRES NGKVLLEDQE LKVEIGKNEA PLAIELGLIN MRSDMTRSMI VSPKLFTNFE KPENFDENSI KIIDMNIIE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaatatta tacacaaaaa caacactcaa ccaacataca aatctttcta ataaaccata caaaattaat gttaatcatt ctctgacaca cattatagga tatgatttat taagaccaat cattgaatct cgtattgatt actacataaa aataaatgga ttagttgatt accttgagca cattaaacat aaaactataa aaccagatct ctttaaaata cacgatattg tagaaggaaa tggtaaaaaa gcactatgtg gacaaaatat ttccgcaata atacataaat tcctatatga ttcagaaagc aaaaacatcc tttctcaagc attaaaaaat attccacagg aagtaacatt taaattagga gaaagtatta taaaagaact aaattataca atagcaggta tggaagaaga aggtgaacgt attattatac taacagacca agaaggcaac ttaaaagaac aatactatgt taaattaata tccattaatt catcatatcc agatgaagtc aatgatatat taatttttaa caatactatt gaaaattatg aaggtactgt caataagata cactgtggag atagtgtaag cgtaaaatat acagtacgag aatcaaatgg aaaagtatta ctagaagatc aagaattaaa agtagaaatt ggcaaaaatg aagctcctct tgcgatagag ttaggactca ttaatatgag atcagatatg acacgatcca tgattgtatc tcctaaactt tttactaatt tcgaaaaacc tgaaaatttt gatgaaaata gtattaaaat aattgatatg aacattattg aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa