SSGCID: Target Page

EhchA.17384.a

Type IV secretion system protein, VirB9

CENTER ID:	EhchA.17384.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.17384.a.A2.GE30845	Mature protein( EhchA.17384.a )		31	270

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.17384.a.A2.PS01011	Mature protein( EhchA.17384.a )		31	270

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.189
RefSeq:	YP_507042.1
UniProt:	Q2GHP1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKRCTFYYKL QMKNVAIIFV LILFSSTVCF AKQEVRSIAQ DNHIKVITYN PHAVHKYTGF YGYQSSIVFD DGEDIDTISM GDSTGWQLMP KGNRLFIKPV ADKADTNATI ITNKRVYYFE FFAEEAQGLD DPRLAYEVRF VYSSTEDFIS EDSPGMVFPV NQPAIPDLTD SDVAKKGLNF DYLVSHNRGS QRIVPLKIFD DNQFTYMQFA QINADLPSIF TVDSEGYESL INFRVSGDYV IIERVSSAFT LRYGSDTVCV FNNKYKKTKR

NT Sequence

ATGAAACGTT GTACATTTTA CTACAAACTA CAGATGAAAA ACGTAGCAAT AATTTTTGTG TTGATTTTAT TCAGCAGTAC AGTCTGTTTT GCTAAGCAAG AAGTACGTTC TATAGCACAA GATAATCATA TCAAAGTTAT TACTTACAAT CCCCATGCTG TACATAAGTA CACAGGGTTT TACGGCTACC AATCTAGTAT AGTGTTTGAC GATGGAGAAG ATATCGACAC CATATCTATG GGTGATTCAA CAGGATGGCA ACTAATGCCA AAAGGTAATA GATTATTTAT TAAACCTGTA GCCGACAAAG CAGATACAAA TGCAACAATA ATTACAAACA AGCGTGTATA CTACTTTGAA TTTTTTGCAG AAGAAGCACA AGGACTCGAT GATCCAAGAT TAGCCTATGA AGTAAGGTTT GTATATTCTT CTACTGAAGA CTTTATTTCA GAAGATAGTC CTGGTATGGT GTTTCCAGTT AACCAACCTG CTATACCAGA CTTAACTGAC AGTGATGTTG CAAAAAAAGG ACTAAATTTT GATTATTTAG TATCACACAA TAGAGGAAGT CAACGAATTG TTCCACTCAA AATTTTTGAC GATAACCAAT TCACTTATAT GCAATTTGCT CAAATTAATG CAGATTTACC ATCAATATTT ACTGTAGATT CTGAAGGATA TGAATCATTG ATTAATTTTA GAGTATCAGG TGATTATGTT ATTATTGAAC GAGTTAGTAG CGCATTCACT TTACGCTATG GTTCAGACAC AGTATGCGTA TTTAATAACA AATACAAGAA AACAAAAAGG

Details for EhchA.17384.a.A2.GE30845

HARVESTED ON:	11/16/2010
SEQUENCED ON:	11/23/2010
EXPECTED MW:	29kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAKQEVRSI AQDNHIKVIT YNPHAVHKYT GFYGYQSSIV FDDGEDIDTI SMGDSTGWQL MPKGNRLFIK PVADKADTNA TIITNKRVYY FEFFAEEAQG LDDPRLAYEV RFVYSSTEDF ISEDSPGMVF PVNQPAIPDL TGSDVAKKGL NFDYLVSHNR GSQRIVPLKI FDDNQFTYMQ FAQINADLPS IFTVDSEGYE SLINFRVSGD YVIIERVSSA FTLRYGSDTV CVFNNKYKKT

Validated NT Sequence

ttgttttctt gtatttgtta ttaaatacgc atactgtgtc tgaaccatag cgtaaagtga atgcgctact aactcgttca ataataacat aatcacctga tactctaaaa ttaatcaatg attcatatcc ttcagaatct acagtaaata ttgatggtaa atctgcatta atttgagcaa attgcatata agtgaattgg ttatcgtcaa aaattttgag tggaacaatt cgttgacttc ctctattgtg tgatactaaa taatcaaaat ttagtccttt ttttgcaaca tcactgccag ttaagtctgg tatagcaggt tggttaactg gaaacaccat accaggacta tcttctgaaa taaagtcttc agtagaagaa tatacaaacc ttacttcata ggctaatctt ggatcatcga gtccttgtgc ttcttctgca aaaaattcaa agtagtatac acgcttgttt gtaattattg ttgcatttgt atctgctttg tcggctacag gtttaataaa taatctatta ccttttggca ttagttgcca tcctgttgaa tcacccatag atatggtgtc gatatcttct ccatcgtcaa acactatact agattggtag ccgtaaaacc ctgtgtactt atgtacagca tggggattgt aagtaataac tttgatatga ttatcttgtg ctatagaacg tacttcttgc ttagccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAKQEVRSI AQDNHIKVIT YNPHAVHKYT GFYGYQSSIV FDDGEDIDTI SMGDSTGWQL MPKGNRLFIK PVADKADTNA TIITNKRVYY FEFFAEEAQG LDDPRLAYEV RFVYSSTEDF ISEDSPGMVF PVNQPAIPDL TDSDVAKKGL NFDYLVSHNR GSQRIVPLKI FDDNQFTYMQ FAQINADLPS IFTVDSEGYE SLINFRVSGD YVIIERVSSA FTLRYGSDTV CVFNNKYKKT KR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctaagcaa gaagtacgtt ctatagcaca agataatcat atcaaagtta ttacttacaa tccccatgct gtacataagt acacagggtt ttacggctac caatctagta tagtgtttga cgatggagaa gatatcgaca ccatatctat gggtgattca acaggatggc aactaatgcc aaaaggtaat agattattta ttaaacctgt agccgacaaa gcagatacaa atgcaacaat aattacaaac aagcgtgtat actactttga attttttgca gaagaagcac aaggactcga tgatccaaga ttagcctatg aagtaaggtt tgtatattct tctactgaag actttatttc agaagatagt cctggtatgg tgtttccagt taaccaacct gctataccag acttaactga cagtgatgtt gcaaaaaaag gactaaattt tgattattta gtatcacaca atagaggaag tcaacgaatt gttccactca aaatttttga cgataaccaa ttcacttata tgcaatttgc tcaaattaat gcagatttac catcaatatt tactgtagat tctgaaggat atgaatcatt gattaatttt agagtatcag gtgattatgt tattattgaa cgagttagta gcgcattcac tttacgctat ggttcagaca cagtatgcgt atttaataac aaatacaaga aaacaaaaag gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for EhchA.17384.a.A2.PS01011

PURIFICATION DATe:	3/1/2011
CONCENTRATION:	8.42mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAKQEVRSI AQDNHIKVIT YNPHAVHKYT GFYGYQSSIV FDDGEDIDTI SMGDSTGWQL MPKGNRLFIK PVADKADTNA TIITNKRVYY FEFFAEEAQG LDDPRLAYEV RFVYSSTEDF ISEDSPGMVF PVNQPAIPDL TDSDVAKKGL NFDYLVSHNR GSQRIVPLKI FDDNQFTYMQ FAQINADLPS IFTVDSEGYE SLINFRVSGD YVIIERVSSA FTLRYGSDTV CVFNNKYKKT KR

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTAAGCAA GAAGTACGTT CTATAGCACA AGATAATCAT ATCAAAGTTA TTACTTACAA TCCCCATGCT GTACATAAGT ACACAGGGTT TTACGGCTAC CAATCTAGTA TAGTGTTTGA CGATGGAGAA GATATCGACA CCATATCTAT GGGTGATTCA ACAGGATGGC AACTAATGCC AAAAGGTAAT AGATTATTTA TTAAACCTGT AGCCGACAAA GCAGATACAA ATGCAACAAT AATTACAAAC AAGCGTGTAT ACTACTTTGA ATTTTTTGCA GAAGAAGCAC AAGGACTCGA TGATCCAAGA TTAGCCTATG AAGTAAGGTT TGTATATTCT TCTACTGAAG ACTTTATTTC AGAAGATAGT CCTGGTATGG TGTTTCCAGT TAACCAACCT GCTATACCAG ACTTAACTGA CAGTGATGTT GCAAAAAAAG GACTAAATTT TGATTATTTA GTATCACACA ATAGAGGAAG TCAACGAATT GTTCCACTCA AAATTTTTGA CGATAACCAA TTCACTTATA TGCAATTTGC TCAAATTAAT GCAGATTTAC CATCAATATT TACTGTAGAT TCTGAAGGAT ATGAATCATT GATTAATTTT AGAGTATCAG GTGATTATGT TATTATTGAA CGAGTTAGTA GCGCATTCAC TTTACGCTAT GGTTCAGACA CAGTATGCGT ATTTAATAAC AAATACAAGA AAACAAAAAG GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA