EhchA.10100.a

serine protease, DO/DeqQ family

CENTER ID: EhchA.10100.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.10100.a.A2.GE27472 Mature protein( EhchA.10100.a ) 22 471
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.10100.a.A2.PW27465 Mature protein( EhchA.10100.a ) 22 471

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.903
RefSeq: YP_507837.1
UniProt: Q2GFE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRFLLLILV LILANVPIGS FADQKAEQYD PRSGFSKLIK ESTPAVVNIS IVHDLIQEQF PLITLEELLR NILEGKPVKK DIPQEVLSAG SGFVVDESGI IVTNYHVVHN AKEVYVTFSD NKSIPAKILG VDPQTDLAVL KVEVNEKLPY LEFGDSDKTM VGDWVVAIGN PFGLGGSASI GIISARARDL NIGTATEFLQ TDAAINKGNS GGPLFNIDGK VIGINTAILS TQKGGGNIGV GFAIPSNNAV SIIKVLSQGK KVEHGWLGVV MQPITEELVE PLQLKEVGGA LITNVVKGSP ASKANLLPGD IILEFNGTKI NSISQLHQLV LRSEADNEVK LLVSRNGSII SILVKIGKFE NPDISENGMP KDAIQSPELG LTVGSVQRNN IYNNIEEEEA KGVVILDIDS TSNASTRNIR KGDIILQINQ SPVNNLEDFK NVMKKVRKNK SVALLISRDN ISAFVTVKLK Q
NT Sequence
ATGAAAAGAT TTCTATTACT AATATTAGTA CTAATATTGG CAAATGTTCC TATAGGTAGT TTTGCTGATC AGAAAGCAGA ACAATATGAT CCAAGGTCAG GCTTTTCTAA ATTAATCAAA GAATCTACAC CAGCAGTAGT TAATATCAGT ATAGTACATG ATTTAATACA GGAACAATTT CCTTTAATAA CTCTCGAAGA ACTTTTAAGG AATATATTAG AAGGCAAGCC AGTAAAAAAA GACATACCAC AAGAAGTATT AAGCGCAGGG TCGGGATTTG TTGTAGATGA ATCAGGTATT ATCGTCACCA ACTATCATGT TGTACACAAT GCAAAAGAAG TTTATGTCAC ATTTAGTGAC AACAAGTCAA TTCCTGCTAA GATTTTAGGA GTTGACCCAC AAACAGACCT AGCAGTGTTA AAAGTTGAAG TTAATGAAAA ACTTCCTTAT CTAGAATTTG GAGATTCTGA CAAGACTATG GTTGGAGACT GGGTAGTTGC CATAGGTAAC CCATTTGGTC TTGGTGGTTC TGCAAGTATT GGTATTATAT CTGCACGCGC AAGAGATCTT AACATTGGCA CAGCAACAGA ATTTTTACAA ACTGATGCTG CAATTAATAA GGGTAACTCT GGTGGTCCTC TATTTAATAT AGATGGTAAA GTAATTGGTA TTAACACAGC AATATTATCT ACACAAAAAG GTGGTGGTAA CATAGGAGTT GGATTTGCTA TCCCATCAAA TAATGCTGTT TCTATAATAA AAGTTTTATC CCAAGGGAAA AAAGTAGAAC ATGGTTGGCT CGGTGTAGTG ATGCAACCGA TAACTGAAGA ACTAGTAGAA CCATTACAAC TAAAAGAAGT GGGTGGAGCT TTAATTACTA ATGTAGTCAA AGGTAGTCCA GCAAGTAAAG CAAACTTGCT TCCAGGGGAT ATTATACTTG AGTTTAATGG TACTAAGATT AATTCAATAT CACAACTACA TCAATTAGTA CTAAGATCAG AAGCAGACAA TGAAGTAAAA TTACTTGTGT CACGTAATGG TAGTATTATA AGTATACTAG TCAAAATAGG GAAATTTGAG AATCCTGATA TTTCAGAAAA CGGAATGCCT AAGGATGCTA TCCAATCCCC GGAGTTAGGG TTAACAGTAG GTAGTGTACA ACGCAATAAC ATATATAATA ATATTGAGGA AGAAGAAGCA AAAGGAGTGG TGATATTAGA TATAGATAGT ACAAGTAATG CTTCAACCAG AAATATAAGA AAAGGCGATA TAATATTACA AATCAATCAA TCACCAGTAA ATAATCTCGA AGATTTTAAA AATGTTATGA AAAAAGTACG TAAAAATAAA TCTGTAGCAT TGTTAATCAG TAGGGATAAT ATATCAGCTT TTGTGACTGT CAAGCTGAAG CAG
Details for EhchA.10100.a.A2.GE27472
HARVESTED ON: data unavailable
SEQUENCED ON: 9/30/2009
EXPECTED MW: 50kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADQKAEQY DPRSGFSKLI KESTPAVVNI SIVHDLIQEQ FPLITLEELL RNILEGKPVK KDIPQEVLSA GSGFVVDESG IIVTNYHVVH NAKEVYVTFS DNKSIPAKIL GVDPQTDLAV LKVEVNEKLP YLEFGDSDKT MVGDWVVAIG NPFGLGGSAS IGIISARARD LNIGTATEFL QTDAAINKGN SGGPLFNIDG KVIGINTAIL STQKGGGNIG VGFAIPSNNA VSIIKVLSQG KKVEHGWLGV VMQPITEELV EPLQLKEVGG ALITNVVKGS PASKANLLPG DIILEFNGTK INSISQLHQL VLRSEADNEV KLLVSRNGSI ISILVKIGKF ENPDISENGM PKDAIQSPEL GLTVGSVQRN NIYNNIEEEE AKGVVILDID STSNASTRNI RKGDIILQIN QSPVNNLEDF KNVMKKVRKN KSVALLISRD NISAFVTVKL
Validated NT Sequence
taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggctgatc agaaagcaga acaatatgat ccaaggtcag gcttttctaa attaatcaaa gaatctacac cagcagtagt taatatcagt atagtacatg atttaataca ggaacaattt cctttaataa ctctcgaaga acttttaagg aatatattag aaggcaagcc agtaaaaaaa gacataccac aagaagtatt aagcgcaggg tcgggatttg ttgtagatga atcaggtatt atcgtcacca actatcatgt tgtacacaat gcaaaagaag tttatgtcac atttagtgac aacaagtcaa ttcctgctaa gattttagga gttgacccac aaacagacct agcagtgtta aaagttgaag ttaatgaaaa acttccttat ctagaatttg gagattctga caagactatg gttggagact gggtagttgc cataggtaac ccatttggtc ttggtggttc tgcaagtatt ggtattatat ctgcacgcgc aagagatctt aacattggca cagcaacaga atttttacaa actgatgctg caattaataa gggtaactct ggtggtcctc tatttaatat agatggtaaa gtaattggta ttaacacagc aatattatct acacaaaaag gtggtggtaa cataggagtt ggatttgcta tcccatcaaa taatgctgtt tctataataa aagttttatc ccaagggaaa aaagtagaac atggttggct cggtgtagtg atgcaaccga taactgaaga actagtagaa ccattacaac taaaagaagt gggtggagct ttaattacta atgtagtcaa aggtagtcca gcaagtaaag caaacttgct tccaggggat attatacttg agtttaatgg tactaagatt aattcaatat cacaactaca tcaattagta ctaagatcag aagcagacaa tgaagtaaaa ttacttgtgt cacgtaatgg tagtattata agtatactag tcaaaatagg gaaatttgag aatcctgata tttcagaaaa cggaatgcct aaggatgcta tccaatcccc ggagttaggg ttaacagtag gtagtgtaca acgcaataac atatataata atattgagga agaagaagca aaaggagtgg tgatattaga tatagatagt acaagtaatg cttcaaccag aaatataaga aaaggcgata taatattaca aatcaatcaa tcaccagtaa ataatctcga agattttaaa aatgttatga aaaaagtacg taaaaataaa tctgtagcat tgttaatcag tagggataat atatcagctt ttgtgactgt caagctga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADQKAEQY DPRSGFSKLI KESTPAVVNI SIVHDLIQEQ FPLITLEELL RNILEGKPVK KDIPQEVLSA GSGFVVDESG IIVTNYHVVH NAKEVYVTFS DNKSIPAKIL GVDPQTDLAV LKVEVNEKLP YLEFGDSDKT MVGDWVVAIG NPFGLGGSAS IGIISARARD LNIGTATEFL QTDAAINKGN SGGPLFNIDG KVIGINTAIL STQKGGGNIG VGFAIPSNNA VSIIKVLSQG KKVEHGWLGV VMQPITEELV EPLQLKEVGG ALITNVVKGS PASKANLLPG DIILEFNGTK INSISQLHQL VLRSEADNEV KLLVSRNGSI ISILVKIGKF ENPDISENGM PKDAIQSPEL GLTVGSVQRN NIYNNIEEEE AKGVVILDID STSNASTRNI RKGDIILQIN QSPVNNLEDF KNVMKKVRKN KSVALLISRD NISAFVTVKL KQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgatcag aaagcagaac aatatgatcc aaggtcaggc ttttctaaat taatcaaaga atctacacca gcagtagtta atatcagtat agtacatgat ttaatacagg aacaatttcc tttaataact ctcgaagaac ttttaaggaa tatattagaa ggcaagccag taaaaaaaga cataccacaa gaagtattaa gcgcagggtc gggatttgtt gtagatgaat caggtattat cgtcaccaac tatcatgttg tacacaatgc aaaagaagtt tatgtcacat ttagtgacaa caagtcaatt cctgctaaga ttttaggagt tgacccacaa acagacctag cagtgttaaa agttgaagtt aatgaaaaac ttccttatct agaatttgga gattctgaca agactatggt tggagactgg gtagttgcca taggtaaccc atttggtctt ggtggttctg caagtattgg tattatatct gcacgcgcaa gagatcttaa cattggcaca gcaacagaat ttttacaaac tgatgctgca attaataagg gtaactctgg tggtcctcta tttaatatag atggtaaagt aattggtatt aacacagcaa tattatctac acaaaaaggt ggtggtaaca taggagttgg atttgctatc ccatcaaata atgctgtttc tataataaaa gttttatccc aagggaaaaa agtagaacat ggttggctcg gtgtagtgat gcaaccgata actgaagaac tagtagaacc attacaacta aaagaagtgg gtggagcttt aattactaat gtagtcaaag gtagtccagc aagtaaagca aacttgcttc caggggatat tatacttgag tttaatggta ctaagattaa ttcaatatca caactacatc aattagtact aagatcagaa gcagacaatg aagtaaaatt acttgtgtca cgtaatggta gtattataag tatactagtc aaaataggga aatttgagaa tcctgatatt tcagaaaacg gaatgcctaa ggatgctatc caatccccgg agttagggtt aacagtaggt agtgtacaac gcaataacat atataataat attgaggaag aagaagcaaa aggagtggtg atattagata tagatagtac aagtaatgct tcaaccagaa atataagaaa aggcgatata atattacaaa tcaatcaatc accagtaaat aatctcgaag attttaaaaa tgttatgaaa aaagtacgta aaaataaatc tgtagcattg ttaatcagta gggataatat atcagctttt gtgactgtca agctgaagca gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for EhchA.10100.a.A2.PW27465
PURIFICATION DATe: 3/1/2010
CONCENTRATION: 25.29mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMADQKAEQY DPRSGFSKLI KESTPAVVNI SIVHDLIQEQ FPLITLEELL RNILEGKPVK KDIPQEVLSA GSGFVVDESG IIVTNYHVVH NAKEVYVTFS DNKSIPAKIL GVDPQTDLAV LKVEVNEKLP YLEFGDSDKT MVGDWVVAIG NPFGLGGSAS IGIISARARD LNIGTATEFL QTDAAINKGN SGGPLFNIDG KVIGINTAIL STQKGGGNIG VGFAIPSNNA VSIIKVLSQG KKVEHGWLGV VMQPITEELV EPLQLKEVGG ALITNVVKGS PASKANLLPG DIILEFNGTK INSISQLHQL VLRSEADNEV KLLVSRNGSI ISILVKIGKF ENPDISENGM PKDAIQSPEL GLTVGSVQRN NIYNNIEEEE AKGVVILDID STSNASTRNI RKGDIILQIN QSPVNNLEDF KNVMKKVRKN KSVALLISRD NISAFVTVKL
Validated NT Sequence
taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggctgatc agaaagcaga acaatatgat ccaaggtcag gcttttctaa attaatcaaa gaatctacac cagcagtagt taatatcagt atagtacatg atttaataca ggaacaattt cctttaataa ctctcgaaga acttttaagg aatatattag aaggcaagcc agtaaaaaaa gacataccac aagaagtatt aagcgcaggg tcgggatttg ttgtagatga atcaggtatt atcgtcacca actatcatgt tgtacacaat gcaaaagaag tttatgtcac atttagtgac aacaagtcaa ttcctgctaa gattttagga gttgacccac aaacagacct agcagtgtta aaagttgaag ttaatgaaaa acttccttat ctagaatttg gagattctga caagactatg gttggagact gggtagttgc cataggtaac ccatttggtc ttggtggttc tgcaagtatt ggtattatat ctgcacgcgc aagagatctt aacattggca cagcaacaga atttttacaa actgatgctg caattaataa gggtaactct ggtggtcctc tatttaatat agatggtaaa gtaattggta ttaacacagc aatattatct acacaaaaag gtggtggtaa cataggagtt ggatttgcta tcccatcaaa taatgctgtt tctataataa aagttttatc ccaagggaaa aaagtagaac atggttggct cggtgtagtg atgcaaccga taactgaaga actagtagaa ccattacaac taaaagaagt gggtggagct ttaattacta atgtagtcaa aggtagtcca gcaagtaaag caaacttgct tccaggggat attatacttg agtttaatgg tactaagatt aattcaatat cacaactaca tcaattagta ctaagatcag aagcagacaa tgaagtaaaa ttacttgtgt cacgtaatgg tagtattata agtatactag tcaaaatagg gaaatttgag aatcctgata tttcagaaaa cggaatgcct aaggatgcta tccaatcccc ggagttaggg ttaacagtag gtagtgtaca acgcaataac atatataata atattgagga agaagaagca aaaggagtgg tgatattaga tatagatagt acaagtaatg cttcaaccag aaatataaga aaaggcgata taatattaca aatcaatcaa tcaccagtaa ataatctcga agattttaaa aatgttatga aaaaagtacg taaaaataaa tctgtagcat tgttaatcag tagggataat atatcagctt ttgtgactgt caagctga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADQKAEQY DPRSGFSKLI KESTPAVVNI SIVHDLIQEQ FPLITLEELL RNILEGKPVK KDIPQEVLSA GSGFVVDESG IIVTNYHVVH NAKEVYVTFS DNKSIPAKIL GVDPQTDLAV LKVEVNEKLP YLEFGDSDKT MVGDWVVAIG NPFGLGGSAS IGIISARARD LNIGTATEFL QTDAAINKGN SGGPLFNIDG KVIGINTAIL STQKGGGNIG VGFAIPSNNA VSIIKVLSQG KKVEHGWLGV VMQPITEELV EPLQLKEVGG ALITNVVKGS PASKANLLPG DIILEFNGTK INSISQLHQL VLRSEADNEV KLLVSRNGSI ISILVKIGKF ENPDISENGM PKDAIQSPEL GLTVGSVQRN NIYNNIEEEE AKGVVILDID STSNASTRNI RKGDIILQIN QSPVNNLEDF KNVMKKVRKN KSVALLISRD NISAFVTVKL KQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCTGATCAG AAAGCAGAAC AATATGATCC AAGGTCAGGC TTTTCTAAAT TAATCAAAGA ATCTACACCA GCAGTAGTTA ATATCAGTAT AGTACATGAT TTAATACAGG AACAATTTCC TTTAATAACT CTCGAAGAAC TTTTAAGGAA TATATTAGAA GGCAAGCCAG TAAAAAAAGA CATACCACAA GAAGTATTAA GCGCAGGGTC GGGATTTGTT GTAGATGAAT CAGGTATTAT CGTCACCAAC TATCATGTTG TACACAATGC AAAAGAAGTT TATGTCACAT TTAGTGACAA CAAGTCAATT CCTGCTAAGA TTTTAGGAGT TGACCCACAA ACAGACCTAG CAGTGTTAAA AGTTGAAGTT AATGAAAAAC TTCCTTATCT AGAATTTGGA GATTCTGACA AGACTATGGT TGGAGACTGG GTAGTTGCCA TAGGTAACCC ATTTGGTCTT GGTGGTTCTG CAAGTATTGG TATTATATCT GCACGCGCAA GAGATCTTAA CATTGGCACA GCAACAGAAT TTTTACAAAC TGATGCTGCA ATTAATAAGG GTAACTCTGG TGGTCCTCTA TTTAATATAG ATGGTAAAGT AATTGGTATT AACACAGCAA TATTATCTAC ACAAAAAGGT GGTGGTAACA TAGGAGTTGG ATTTGCTATC CCATCAAATA ATGCTGTTTC TATAATAAAA GTTTTATCCC AAGGGAAAAA AGTAGAACAT GGTTGGCTCG GTGTAGTGAT GCAACCGATA ACTGAAGAAC TAGTAGAACC ATTACAACTA AAAGAAGTGG GTGGAGCTTT AATTACTAAT GTAGTCAAAG GTAGTCCAGC AAGTAAAGCA AACTTGCTTC CAGGGGATAT TATACTTGAG TTTAATGGTA CTAAGATTAA TTCAATATCA CAACTACATC AATTAGTACT AAGATCAGAA GCAGACAATG AAGTAAAATT ACTTGTGTCA CGTAATGGTA GTATTATAAG TATACTAGTC AAAATAGGGA AATTTGAGAA TCCTGATATT TCAGAAAACG GAATGCCTAA GGATGCTATC CAATCCCCGG AGTTAGGGTT AACAGTAGGT AGTGTACAAC GCAATAACAT ATATAATAAT ATTGAGGAAG AAGAAGCAAA AGGAGTGGTG ATATTAGATA TAGATAGTAC AAGTAATGCT TCAACCAGAA ATATAAGAAA AGGCGATATA ATATTACAAA TCAATCAATC ACCAGTAAAT AATCTCGAAG ATTTTAAAAA TGTTATGAAA AAAGTACGTA AAAATAAATC TGTAGCATTG TTAATCAGTA GGGATAATAT ATCAGCTTTT GTGACTGTCA AGCTGAAGCA GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA