SSGCID: Target Page

EhchA.01603.a

thioredoxin-disulfide reductase

CENTER ID:	EhchA.01603.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01603.a.A1.GE27464	Full length( EhchA.01603.a )		1	318

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01603.a.A1.PW27457	Full length( EhchA.01603.a )		1	318

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.643
RefSeq:	YP_507537.1
UniProt:	Q2GG96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTQTYNTKVL IIGSGAAGCT AAIYAARANL KPILITGMCP GGQLTITTDV ENFPGFAHAV QGPDLMEQMK QQAHNSGAQI ISDEIKEIHS DVYPFKCIGI FGDQYIADSI IIATGAQAKW LNIKSEETFK GRGVSACATC DGTFFAGSDI AVIGGGNTAV EEALYLTRYA TKVFLIHRRD TLRAEPIMQE RLFSNDKIQV IWNSVVEEIL GNKESGNVEA IALKSVKTGD ITTISVKGVF IAIGHTPNTQ ILTTKDNGNI VDLDNEGYII TKPGSTVTSH PGIFAAGDVQ DKIYRQAVVA AGSGCMAALE AAKFLSEQ

NT Sequence

ATGACACAAA CATATAATAC AAAAGTTTTA ATTATAGGAT CTGGAGCAGC AGGTTGTACT GCTGCTATAT ATGCTGCTCG TGCAAACTTA AAACCTATAT TAATAACTGG AATGTGCCCC GGGGGACAGC TTACAATTAC TACAGATGTT GAAAATTTTC CAGGATTTGC ACATGCAGTA CAAGGTCCAG ATTTAATGGA ACAAATGAAA CAACAAGCTC ATAACTCCGG AGCTCAGATT ATATCTGACG AAATAAAAGA AATACATTCA GATGTATACC CTTTTAAATG TATAGGAATA TTTGGTGATC AGTACATTGC AGATAGTATT ATAATTGCAA CAGGAGCTCA AGCAAAATGG CTCAACATAA AAAGCGAAGA AACCTTTAAA GGTAGAGGTG TATCTGCATG TGCTACATGT GATGGAACGT TTTTCGCTGG TAGTGATATT GCCGTAATAG GAGGAGGTAA TACAGCTGTA GAAGAAGCAT TATATCTAAC AAGATATGCA ACAAAAGTAT TTTTAATTCA TAGAAGAGAT ACTCTACGTG CTGAACCTAT AATGCAAGAA CGATTATTCA GTAATGATAA AATACAAGTT ATATGGAACA GCGTTGTAGA AGAAATACTA GGAAATAAGG AAAGTGGGAA TGTAGAAGCT ATAGCATTAA AGTCTGTAAA AACTGGAGAC ATCACTACTA TTTCCGTAAA AGGAGTATTT ATTGCTATAG GACATACTCC AAACACACAA ATACTAACAA CCAAAGATAA TGGGAATATA GTAGATTTAG ATAACGAAGG ATATATCATT ACTAAACCTG GTAGTACAGT AACTAGTCAC CCTGGAATCT TTGCTGCAGG TGATGTACAA GATAAAATAT ATAGACAAGC TGTTGTTGCA GCAGGATCTG GGTGTATGGC TGCATTGGAA GCTGCCAAAT TTTTATCAGA GCAA

Details for EhchA.01603.a.A1.GE27464

HARVESTED ON:	9/15/2009
SEQUENCED ON:	9/30/2009
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMTQTYNT KVLIIGSGAA GCTAAIYAAR ANLKPILITG MCPGGQLTIT TDVENFPGFA HAVQGPDLME QMKQQAHNSG AQIISDEIKE IHSDVYPFKC IGIFGDQYIA DSIIIATGAQ AKWLNIKSEE TFKGRGVSAC ATCDGTFFAG SDIAVIGGGN TAVEEALYLT RYATKVFLIH RRDTLRAEPI MQERLFSNDK IQVIWNSVVE EILGNKESGN VEAIALKSVK TGDITTISVK GVFIAIGHTP NTQILTTKDN GNIVDLDNEG YIITKPGSTV TSHPGIFAAG DVQDKIYRQA VVAAGSGCMA ALEAAKFLS

Validated NT Sequence

ctgataaaaa tttggcagct tccaatgcag ccatacaccc agatcctgct gcaacaacag cttgtctata tattttatct tgtacatcac ctgcagcaaa gattccaggg tgactagtta ctgtactacc aggtttagta atgatatatc cttcgttatc taaatctact atattcccat tatctttggt tgttagtatt tgtgtgtttg gagtatgtcc tatagcaata aatactcctt ttacggaaat agtagtgatg tctccagttt ttacagactt taatgctata gcttctacat tcccactttc cttatttcct agtatttctt ctacaacgct gttccatata acttgtattt tatcattact gaataatcgt tcttgcatta taggttcagc acgtagagta tctcttctat gaattaaaaa tacttttgtt gcatatcttg ttagatataa tgcttcttct acagctgtat tacctcctcc tattacggca atatcactac cagcgaaaaa cgttccatca catgtagcac atgcagatac acctctacct ttaaaggttt cttcgctttt tatgttgagc cattttgctt gagctcctgt tgcaattata atactatctg caatgtactg atcaccaaat attcctatac atttaaaagg gtatacatct gaatgtattt cttttatttc gtcagatata atctgagctc cggagttatg agcttgttgt ttcatttgtt ccattaaatc tggaccttgt actgcatgtg caaatcctgg aaaattttca acatctgtag taattgtaag ctgtcccccg gggcacattc cagttattaa tataggtttt aagtttgcac gagcagcata tatagcagca gtacaacctg ctgctccaga tcctataatt aaaacttttg tattatatgt ttgtgtcatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatn gtnat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTQTYNTKV LIIGSGAAGC TAAIYAARAN LKPILITGMC PGGQLTITTD VENFPGFAHA VQGPDLMEQM KQQAHNSGAQ IISDEIKEIH SDVYPFKCIG IFGDQYIADS IIIATGAQAK WLNIKSEETF KGRGVSACAT CDGTFFAGSD IAVIGGGNTA VEEALYLTRY ATKVFLIHRR DTLRAEPIMQ ERLFSNDKIQ VIWNSVVEEI LGNKESGNVE AIALKSVKTG DITTISVKGV FIAIGHTPNT QILTTKDNGN IVDLDNEGYI ITKPGSTVTS HPGIFAAGDV QDKIYRQAVV AAGSGCMAAL EAAKFLSEQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacaaaca tataatacaa aagttttaat tataggatct ggagcagcag gttgtactgc tgctatatat gctgctcgtg caaacttaaa acctatatta ataactggaa tgtgccccgg gggacagctt acaattacta cagatgttga aaattttcca ggatttgcac atgcagtaca aggtccagat ttaatggaac aaatgaaaca acaagctcat aactccggag ctcagattat atctgacgaa ataaaagaaa tacattcaga tgtataccct tttaaatgta taggaatatt tggtgatcag tacattgcag atagtattat aattgcaaca ggagctcaag caaaatggct caacataaaa agcgaagaaa cctttaaagg tagaggtgta tctgcatgtg ctacatgtga tggaacgttt ttcgctggta gtgatattgc cgtaatagga ggaggtaata cagctgtaga agaagcatta tatctaacaa gatatgcaac aaaagtattt ttaattcata gaagagatac tctacgtgct gaacctataa tgcaagaacg attattcagt aatgataaaa tacaagttat atggaacagc gttgtagaag aaatactagg aaataaggaa agtgggaatg tagaagctat agcattaaag tctgtaaaaa ctggagacat cactactatt tccgtaaaag gagtatttat tgctatagga catactccaa acacacaaat actaacaacc aaagataatg ggaatatagt agatttagat aacgaaggat atatcattac taaacctggt agtacagtaa ctagtcaccc tggaatcttt gctgcaggtg atgtacaaga taaaatatat agacaagctg ttgttgcagc aggatctggg tgtatggctg cattggaagc tgccaaattt ttatcagagc aaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa

Details for EhchA.01603.a.A1.PW27457

PURIFICATION DATe:	11/2/2009
CONCENTRATION:	21.63mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	23
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACACAAACA TATAATACAA AAGTTTTAAT TATAGGATCT GGAGCAGCAG GTTGTACTGC TGCTATATAT GCTGCTCGTG CAAACTTAAA ACCTATATTA ATAACTGGAA TGTGCCCCGG GGGACAGCTT ACAATTACTA CAGATGTTGA AAATTTTCCA GGATTTGCAC ATGCAGTACA AGGTCCAGAT TTAATGGAAC AAATGAAACA ACAAGCTCAT AACTCCGGAG CTCAGATTAT ATCTGACGAA ATAAAAGAAA TACATTCAGA TGTATACCCT TTTAAATGTA TAGGAATATT TGGTGATCAG TACATTGCAG ATAGTATTAT AATTGCAACA GGAGCTCAAG CAAAATGGCT CAACATAAAA AGCGAAGAAA CCTTTAAAGG TAGAGGTGTA TCTGCATGTG CTACATGTGA TGGAACGTTT TTCGCTGGTA GTGATATTGC CGTAATAGGA GGAGGTAATA CAGCTGTAGA AGAAGCATTA TATCTAACAA GATATGCAAC AAAAGTATTT TTAATTCATA GAAGAGATAC TCTACGTGCT GAACCTATAA TGCAAGAACG ATTATTCAGT AATGATAAAA TACAAGTTAT ATGGAACAGC GTTGTAGAAG AAATACTAGG AAATAAGGAA AGTGGGAATG TAGAAGCTAT AGCATTAAAG TCTGTAAAAA CTGGAGACAT CACTACTATT TCCGTAAAAG GAGTATTTAT TGCTATAGGA CATACTCCAA ACACACAAAT ACTAACAACC AAAGATAATG GGAATATAGT AGATTTAGAT AACGAAGGAT ATATCATTAC TAAACCTGGT AGTACAGTAA CTAGTCACCC TGGAATCTTT GCTGCAGGTG ATGTACAAGA TAAAATATAT AGACAAGCTG TTGTTGCAGC AGGATCTGGG TGTATGGCTG CATTGGAAGC TGCCAAATTT TTATCAGAGC AAAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA