EhchA.01553.a

quinone oxidoreductase

CENTER ID: EhchA.01553.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01553.a.A1.GE27466 Full length( EhchA.01553.a ) 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.325
RefSeq: YP_507205.1
UniProt: Q2GH78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVKVIIIDKT GSAEVLKYVN HNIGEPKDDE VLIKHTAIGL NRYDLECRNG MRKNKKLPAV LGVEAVGVIE RLGKDVEVFN IGDRVGYCTV SGGAYSEKRL IKQKYLVRIP DDITDQVAAA VLFKGMTAHY LTHLVYAIRP NTFALVHGAT GGVGQILCQW ASYKGCKVIG VISSDDRANI ALRAGCSYVV NNNSKNFVEQ IMEITHGSGV NVVYDCIGDL ASKVSFESLC MFGIYVSYGQ ISGPISNVSA SMLSSRSLFF TSPLVFHYKR SSFDLAMTAM ELFEVIRRNY IKVGINKIYK FGDIVTAHQD LENRKLTGSN IIEL
NT Sequence
ATGGTAAAAG TTATTATTAT TGATAAAACT GGTAGTGCTG AAGTTTTAAA ATATGTTAAT CATAATATTG GTGAACCTAA AGATGATGAG GTATTGATAA AACATACAGC TATTGGCTTA AATCGCTACG ATCTTGAGTG CCGTAATGGT ATGCGTAAAA ATAAAAAACT TCCTGCTGTT TTAGGAGTTG AGGCAGTAGG TGTTATAGAG AGATTGGGTA AAGATGTTGA AGTGTTTAAT ATTGGTGATA GAGTAGGTTA TTGTACTGTA TCTGGAGGGG CTTATTCTGA AAAAAGACTT ATTAAACAAA AATATTTAGT TAGAATACCT GATGATATTA CTGATCAAGT AGCAGCTGCG GTATTATTTA AAGGTATGAC AGCGCATTAT CTTACACACT TAGTTTATGC TATACGTCCT AATACTTTTG CTTTAGTGCA TGGAGCAACA GGTGGTGTAG GCCAAATATT ATGTCAGTGG GCTAGTTATA AAGGATGTAA AGTTATAGGT GTAATTAGTT CTGATGATAG GGCTAATATT GCCTTGCGTG CTGGATGTTC ATATGTAGTA AATAATAATA GTAAAAATTT TGTTGAGCAG ATCATGGAAA TTACTCACGG TTCTGGAGTT AATGTAGTTT ATGACTGTAT AGGTGATTTA GCTAGTAAAG TATCTTTTGA ATCTCTTTGT ATGTTTGGTA TTTATGTTTC TTATGGCCAG ATATCAGGAC CTATTTCAAA TGTTAGTGCT TCTATGCTAA GTTCCAGATC ATTGTTTTTT ACCTCTCCTT TGGTGTTCCA CTATAAACGT TCTAGTTTTG ATTTGGCGAT GACAGCTATG GAGCTTTTTG AAGTGATAAG AAGAAATTAT ATAAAAGTTG GGATAAATAA GATTTATAAG TTTGGTGATA TAGTAACTGC ACATCAGGAT TTGGAAAATA GAAAACTTAC TGGTTCAAAT ATTATTGAAC TG
Details for EhchA.01553.a.A1.GE27466
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMVKVIII DKTGSAEVLK YVNHNIGEPK DDEVLIKHTA IGLNRYDLEC RNGMRKNKKL PAVLGVEAVG VIERLGKDVE VFNIGDRVGY CTVSGGAYSE KRLIKQKYLV RIPDDITDQV AAAVLFKGMT AHYLTHLVYA IRPNTFALVH GATGGVGQIL CQWASYKGCK VIGVISSDDR ANIALRAGCS YVVNNNSKNF VEQIMEITHG SGVNVVYDCI GDLASKVSFE SLCMFGIYVS YGQISGPISN VSASMLSSRS LFFTSPLVFH YKRSSFDLAM TAMELFEVIR RNYIKVGINK IYKFGDIVTA HQDLENRKLT GSNII
Validated NT Sequence
caataatatt tgaaccagta agttttctat tttccaaatc ctgatgtgca gttactatat caccaaactt ataaatctta tttatcccaa cttttatata atttcttctt atcacttcaa aaagctccat agctgtcatc gccaaatcaa aactagaacg tttatagtgg aacaccaaag gagaggtaaa aaacaatgat ctggaactta gcatagaagc actaacattt gaaataggtc ctgatatctg gccataagaa acataaatac caaacataca aagagattca aaagatactt tactagctaa atcacctata cagtcataaa ctacattaac tccagaaccg tgagtaattt ccatgatctg ctcaacaaaa tttttactat tattatttac tacatatgaa catccagcac gcaaggcaat attagcccta tcatcagaac taattacacc tataacttta catcctttat aactagccca ctgacataat atttggccta caccacctgt tgctccatgc actaaagcaa aagtattagg acgtatagca taaactaagt gtgtaagata atgcgctgtc atacctttaa ataataccgc agctgctact tgatcagtaa tatcatcagg tattctaact aaatattttt gtttaataag tcttttttca gaataagccc ctccagatac agtacaataa cctactctat caccaatatt aaacacttca acatctttac ccaatctctc tataacacct actgcctcaa ctcctaaaac agcaggaagt tttttatttt tacgcatacc attacggcac tcaagatcgt agcgatttaa gccaatagct gtatgtttta tcaatacctc atcatcttta ggttcaccaa tattatgatt aacatatttt aaaacttcag cactaccagt tttatcaata ataataactt ttaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKVIIIDK TGSAEVLKYV NHNIGEPKDD EVLIKHTAIG LNRYDLECRN GMRKNKKLPA VLGVEAVGVI ERLGKDVEVF NIGDRVGYCT VSGGAYSEKR LIKQKYLVRI PDDITDQVAA AVLFKGMTAH YLTHLVYAIR PNTFALVHGA TGGVGQILCQ WASYKGCKVI GVISSDDRAN IALRAGCSYV VNNNSKNFVE QIMEITHGSG VNVVYDCIGD LASKVSFESL CMFGIYVSYG QISGPISNVS ASMLSSRSLF FTSPLVFHYK RSSFDLAMTA MELFEVIRRN YIKVGINKIY KFGDIVTAHQ DLENRKLTGS NIIEL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtaaaagtt attattattg ataaaactgg tagtgctgaa gttttaaaat atgttaatca taatattggt gaacctaaag atgatgaggt attgataaaa catacagcta ttggcttaaa tcgctacgat cttgagtgcc gtaatggtat gcgtaaaaat aaaaaacttc ctgctgtttt aggagttgag gcagtaggtg ttatagagag attgggtaaa gatgttgaag tgtttaatat tggtgataga gtaggttatt gtactgtatc tggaggggct tattctgaaa aaagacttat taaacaaaaa tatttagtta gaatacctga tgatattact gatcaagtag cagctgcggt attatttaaa ggtatgacag cgcattatct tacacactta gtttatgcta tacgtcctaa tacttttgct ttagtgcatg gagcaacagg tggtgtaggc caaatattat gtcagtgggc tagttataaa ggatgtaaag ttataggtgt aattagttct gatgataggg ctaatattgc cttgcgtgct ggatgttcat atgtagtaaa taataatagt aaaaattttg ttgagcagat catggaaatt actcacggtt ctggagttaa tgtagtttat gactgtatag gtgatttagc tagtaaagta tcttttgaat ctctttgtat gtttggtatt tatgtttctt atggccagat atcaggacct atttcaaatg ttagtgcttc tatgctaagt tccagatcat tgttttttac ctctcctttg gtgttccact ataaacgttc tagttttgat ttggcgatga cagctatgga gctttttgaa gtgataagaa gaaattatat aaaagttggg ataaataaga tttataagtt tggtgatata gtaactgcac atcaggattt ggaaaataga aaacttactg gttcaaatat tattgaactg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa