EhchA.01528.a

DNA-directed RNA polymerase subunit alpha

CENTER ID: EhchA.01528.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01528.a.A1.GE27450 Full length( EhchA.01528.a ) 1 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01528.a.A1.PW27443 Full length( EhchA.01528.a ) 1 341

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.369
RefSeq: YP_507250.1
UniProt: Q2GH33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDHWSKLTK PSSIKVVPSG ASPNKADLII EPLESGFALT LGNALRRVMM SSLRGFAVYG VEIENVLHEF TSISGVREDV TDILLNISMM RVKLSGMNSK VLSLKVKGPC EVRSGMIPDT DDCIILNKDL LICTLDQGVD FNIKMYVNAG KGYVPAVKRK SVNKLGDIPI NFIATNALYS PIKKASFKVE SSRIGQFTDY DRLIMSVETD GSILPDEAVA LAARILQDQF QPFINFDETD EPHKKVDAKD TLPYDSNLLR KVDELELSVR SYNCLKNDNI TYIGDLVQKT ESDMLRTPNF GRKSLNEINE LLASMNLHLG MKIANWPPES IESLSKQYSE E
NT Sequence
ATGAGTGATC ATTGGAGTAA ATTAACTAAG CCTTCTTCTA TTAAGGTAGT TCCTAGTGGT GCTTCTCCAA ATAAGGCAGA TTTAATTATT GAACCTCTTG AGAGTGGTTT TGCTTTAACT CTAGGTAATG CTTTAAGAAG AGTTATGATG TCTTCTTTGC GTGGTTTTGC TGTTTATGGT GTTGAAATAG AGAATGTTTT ACACGAATTT ACTTCGATTT CTGGGGTTAG AGAAGATGTA ACAGATATTC TTTTAAATAT TAGCATGATG AGAGTAAAGC TTTCTGGTAT GAATAGTAAA GTTTTGTCTC TTAAAGTTAA AGGACCATGT GAAGTAAGAT CTGGAATGAT ACCTGACACT GATGATTGCA TTATACTCAA TAAGGATTTG TTAATTTGCA CTTTAGATCA AGGTGTAGAT TTTAATATAA AAATGTATGT AAATGCTGGT AAAGGTTATG TTCCTGCTGT AAAGCGTAAG TCAGTTAATA AACTTGGTGA TATACCAATA AATTTCATTG CAACTAATGC TCTTTATAGC CCTATAAAAA AGGCGTCTTT TAAAGTAGAA AGTAGTCGTA TTGGTCAATT TACAGATTAT GATCGATTGA TTATGTCTGT GGAAACAGAT GGGTCTATTT TACCTGATGA AGCAGTAGCA TTAGCTGCAA GAATTTTACA AGATCAATTT CAACCATTTA TTAATTTTGA TGAAACTGAT GAGCCTCATA AGAAAGTTGA TGCTAAAGAT ACATTGCCTT ATGATTCTAA TCTGTTACGT AAAGTTGATG AGTTAGAACT TTCAGTAAGG TCTTATAATT GTTTGAAAAA TGATAATATT ACTTATATAG GAGATTTAGT CCAAAAGACT GAATCTGATA TGTTAAGAAC TCCAAATTTT GGTAGAAAGT CGCTTAATGA GATTAATGAG CTTTTAGCAA GTATGAATTT GCATTTAGGA ATGAAAATTG CGAATTGGCC TCCTGAATCC ATTGAGAGTT TAAGTAAACA ATATAGTGAA GAA
Details for EhchA.01528.a.A1.GE27450
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDHWSKLT KPSSIKVVPS GASPNKADLI IEPLESGFAL TLGNALRRVM MSSLRGFAVY GVEIENVLHE FTSISGVRED VTDILLNISM MRVKLSGMNS KVLSLKVKGP CEVRSGMIPD TDDCIILNKD LLICTLDQGV DFNIKMYVNA GKGYVPAVKR KSVNKLGDIP INFIATNALY SPIKKASFKV ESSRIGQFTD YDRLIMSVET DGSILPDEAV ALAARILQDQ FQPFINFDET DEPHKKVDAK DTLPYDSNLL RKVDELELSV RSYNCLKNDN ITYIGDLVQK TESDMLRTPN FGRKSLNEIN ELLASMNLHL GMKIANWPPE SIESLSKQYS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg atcattggag taaattaact aagccttctt ctattaaggt agttcctagt ggtgcttctc caaataaggc agatttaatt attgaacctc ttgagagtgg ttttgcttta actctaggta atgctttaag aagagttatg atgtcttctt tgcgtggttt tgctgtttat ggtgttgaaa tagagaatgt tttacacgaa tttacttcga tttctggggt tagagaagat gtaacagata ttcttttaaa tattagcatg atgagagtaa agctttctgg tatgaatagt aaagttttgt ctcttaaagt taaaggacca tgtgaagtaa gatctggaat gatacctgac actgatgatt gcattatact caataaggat ttgttaattt gcactttaga tcaaggtgta gattttaata taaaaatgta tgtaaatgct ggtaaaggtt atgttcctgc tgtaaagcgt aagtcagtta ataaacttgg tgatatacca ataaatttca ttgcaactaa tgctctttat agccctataa aaaaggcgtc ttttaaagta gaaagtagtc gtattggtca atttacagat tatgatcgat tgattatgtc tgtggaaaca gatgggtcta ttttacctga tgaagcagta gcattagctg caagaatttt acaagatcaa tttcaaccat ttattaattt tgatgaaact gatgagcctc ataagaaagt tgatgctaaa gatacattgc cttatgattc taatctgtta cgtaaagttg atgagttaga actttcagta aggtcttata attgtttgaa aaatgataat attacttata taggagattt agtccaaaag actgaatctg atatgttaag aactccaaat tttggtagaa agtcgcttaa tgagattaat gagcttttag caagtatgaa tttgcattta ggaatgaaaa ttgcgaattg gcctcctgaa tccattgaga gtttaagtaa acaatatagt g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDHWSKLT KPSSIKVVPS GASPNKADLI IEPLESGFAL TLGNALRRVM MSSLRGFAVY GVEIENVLHE FTSISGVRED VTDILLNISM MRVKLSGMNS KVLSLKVKGP CEVRSGMIPD TDDCIILNKD LLICTLDQGV DFNIKMYVNA GKGYVPAVKR KSVNKLGDIP INFIATNALY SPIKKASFKV ESSRIGQFTD YDRLIMSVET DGSILPDEAV ALAARILQDQ FQPFINFDET DEPHKKVDAK DTLPYDSNLL RKVDELELSV RSYNCLKNDN ITYIGDLVQK TESDMLRTPN FGRKSLNEIN ELLASMNLHL GMKIANWPPE SIESLSKQYS EE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagtgatcat tggagtaaat taactaagcc ttcttctatt aaggtagttc ctagtggtgc ttctccaaat aaggcagatt taattattga acctcttgag agtggttttg ctttaactct aggtaatgct ttaagaagag ttatgatgtc ttctttgcgt ggttttgctg tttatggtgt tgaaatagag aatgttttac acgaatttac ttcgatttct ggggttagag aagatgtaac agatattctt ttaaatatta gcatgatgag agtaaagctt tctggtatga atagtaaagt tttgtctctt aaagttaaag gaccatgtga agtaagatct ggaatgatac ctgacactga tgattgcatt atactcaata aggatttgtt aatttgcact ttagatcaag gtgtagattt taatataaaa atgtatgtaa atgctggtaa aggttatgtt cctgctgtaa agcgtaagtc agttaataaa cttggtgata taccaataaa tttcattgca actaatgctc tttatagccc tataaaaaag gcgtctttta aagtagaaag tagtcgtatt ggtcaattta cagattatga tcgattgatt atgtctgtgg aaacagatgg gtctatttta cctgatgaag cagtagcatt agctgcaaga attttacaag atcaatttca accatttatt aattttgatg aaactgatga gcctcataag aaagttgatg ctaaagatac attgccttat gattctaatc tgttacgtaa agttgatgag ttagaacttt cagtaaggtc ttataattgt ttgaaaaatg ataatattac ttatatagga gatttagtcc aaaagactga atctgatatg ttaagaactc caaattttgg tagaaagtcg cttaatgaga ttaatgagct tttagcaagt atgaatttgc atttaggaat gaaaattgcg aattggcctc ctgaatccat tgagagttta agtaaacaat atagtgaaga aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for EhchA.01528.a.A1.PW27443
PURIFICATION DATe: 10/26/2009
CONCENTRATION: 22.28mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSDHWSKLT KPSSIKVVPS GASPNKADLI IEPLESGFAL TLGNALRRVM MSSLRGFAVY GVEIENVLHE FTSISGVRED VTDILLNISM MRVKLSGMNS KVLSLKVKGP CEVRSGMIPD TDDCIILNKD LLICTLDQGV DFNIKMYVNA GKGYVPAVKR KSVNKLGDIP INFIATNALY SPIKKASFKV ESSRIGQFTD YDRLIMSVET DGSILPDEAV ALAARILQDQ FQPFINFDET DEPHKKVDAK DTLPYDSNLL RKVDELELSV RSYNCLKNDN ITYIGDLVQK TESDMLRTPN FGRKSLNEIN ELLASMNLHL GMKIANWPPE SIESLSKQYS
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgagtg atcattggag taaattaact aagccttctt ctattaaggt agttcctagt ggtgcttctc caaataaggc agatttaatt attgaacctc ttgagagtgg ttttgcttta actctaggta atgctttaag aagagttatg atgtcttctt tgcgtggttt tgctgtttat ggtgttgaaa tagagaatgt tttacacgaa tttacttcga tttctggggt tagagaagat gtaacagata ttcttttaaa tattagcatg atgagagtaa agctttctgg tatgaatagt aaagttttgt ctcttaaagt taaaggacca tgtgaagtaa gatctggaat gatacctgac actgatgatt gcattatact caataaggat ttgttaattt gcactttaga tcaaggtgta gattttaata taaaaatgta tgtaaatgct ggtaaaggtt atgttcctgc tgtaaagcgt aagtcagtta ataaacttgg tgatatacca ataaatttca ttgcaactaa tgctctttat agccctataa aaaaggcgtc ttttaaagta gaaagtagtc gtattggtca atttacagat tatgatcgat tgattatgtc tgtggaaaca gatgggtcta ttttacctga tgaagcagta gcattagctg caagaatttt acaagatcaa tttcaaccat ttattaattt tgatgaaact gatgagcctc ataagaaagt tgatgctaaa gatacattgc cttatgattc taatctgtta cgtaaagttg atgagttaga actttcagta aggtcttata attgtttgaa aaatgataat attacttata taggagattt agtccaaaag actgaatctg atatgttaag aactccaaat tttggtagaa agtcgcttaa tgagattaat gagcttttag caagtatgaa tttgcattta ggaatgaaaa ttgcgaattg gcctcctgaa tccattgaga gtttaagtaa acaatatagt g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSDHWSKLT KPSSIKVVPS GASPNKADLI IEPLESGFAL TLGNALRRVM MSSLRGFAVY GVEIENVLHE FTSISGVRED VTDILLNISM MRVKLSGMNS KVLSLKVKGP CEVRSGMIPD TDDCIILNKD LLICTLDQGV DFNIKMYVNA GKGYVPAVKR KSVNKLGDIP INFIATNALY SPIKKASFKV ESSRIGQFTD YDRLIMSVET DGSILPDEAV ALAARILQDQ FQPFINFDET DEPHKKVDAK DTLPYDSNLL RKVDELELSV RSYNCLKNDN ITYIGDLVQK TESDMLRTPN FGRKSLNEIN ELLASMNLHL GMKIANWPPE SIESLSKQYS EE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGTGATCAT TGGAGTAAAT TAACTAAGCC TTCTTCTATT AAGGTAGTTC CTAGTGGTGC TTCTCCAAAT AAGGCAGATT TAATTATTGA ACCTCTTGAG AGTGGTTTTG CTTTAACTCT AGGTAATGCT TTAAGAAGAG TTATGATGTC TTCTTTGCGT GGTTTTGCTG TTTATGGTGT TGAAATAGAG AATGTTTTAC ACGAATTTAC TTCGATTTCT GGGGTTAGAG AAGATGTAAC AGATATTCTT TTAAATATTA GCATGATGAG AGTAAAGCTT TCTGGTATGA ATAGTAAAGT TTTGTCTCTT AAAGTTAAAG GACCATGTGA AGTAAGATCT GGAATGATAC CTGACACTGA TGATTGCATT ATACTCAATA AGGATTTGTT AATTTGCACT TTAGATCAAG GTGTAGATTT TAATATAAAA ATGTATGTAA ATGCTGGTAA AGGTTATGTT CCTGCTGTAA AGCGTAAGTC AGTTAATAAA CTTGGTGATA TACCAATAAA TTTCATTGCA ACTAATGCTC TTTATAGCCC TATAAAAAAG GCGTCTTTTA AAGTAGAAAG TAGTCGTATT GGTCAATTTA CAGATTATGA TCGATTGATT ATGTCTGTGG AAACAGATGG GTCTATTTTA CCTGATGAAG CAGTAGCATT AGCTGCAAGA ATTTTACAAG ATCAATTTCA ACCATTTATT AATTTTGATG AAACTGATGA GCCTCATAAG AAAGTTGATG CTAAAGATAC ATTGCCTTAT GATTCTAATC TGTTACGTAA AGTTGATGAG TTAGAACTTT CAGTAAGGTC TTATAATTGT TTGAAAAATG ATAATATTAC TTATATAGGA GATTTAGTCC AAAAGACTGA ATCTGATATG TTAAGAACTC CAAATTTTGG TAGAAAGTCG CTTAATGAGA TTAATGAGCT TTTAGCAAGT ATGAATTTGC ATTTAGGAAT GAAAATTGCG AATTGGCCTC CTGAATCCAT TGAGAGTTTA AGTAAACAAT ATAGTGAAGA AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA