EhchA.01521.a

glutamyl-tRNA synthetase

CENTER ID:	EhchA.01521.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01521.a.A1.GE27446	Full length( EhchA.01521.a )		1	469

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01521.a.A1.PS00391	Full length( EhchA.01521.a )		1	469

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_127621
PATRIC ID:	fig\|205920.11.peg.523
RefSeq:	YP_507415.1
UniProt:	Q2GGL8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSHNVITRFA PSPTGHLHLG GARTALFNWL YAKHNNGKFL LRIEDTDKKR SSKELIDSII NAMSWLKIPY DGEIVLQSKN ISRHIEIANQ LILNNKAYYC YCSEEEINKE KEEFSKKGLY YKHNCIWKNK NFTIDNLTRV IRLRSPTEGV TSFDDKVYGN ITVSNTQLDD MVLLRSDNTP TYLLSVVVDD HDMNITHIIR GTDHLTNTAR QLLIYNALEW NPPKFAHIPL IHDEDGNKLS KRHQAIGIHE YKNLGILPEA ISNYLLRMGW SHEDDEIISM DQAIKWFSIK NIGQSPARLD NKKLEFLNNH YISLTEDEVI LNMIIPIIEK KIGYMLNEVK KGYLLKGLYE LKKRTKNLVN LANESLFYVE DVPISIDQEA SAIIKDYKHV FSILYNNLSR ISEKEWNNSI LTSTIKNISQ NLDIKISNIY HCLRASIVGR MNAPSIIEIM INLQQEECLK RIKYAQNIE

NT Sequence

ATGTCACATA ATGTAATAAC TCGATTTGCC CCTTCACCAA CTGGACATTT ACATTTAGGA GGAGCAAGAA CAGCTCTATT CAACTGGCTA TATGCTAAAC ATAACAATGG AAAATTTTTA TTACGCATTG AAGATACTGA TAAAAAAAGA TCATCTAAAG AACTTATCGA CTCTATTATT AATGCAATGT CTTGGTTAAA AATTCCTTAT GATGGGGAAA TAGTATTACA ATCAAAAAAT ATATCTAGAC ATATTGAAAT TGCAAATCAA CTAATATTAA ACAATAAAGC ATATTACTGT TATTGTTCTG AGGAAGAAAT AAATAAAGAA AAAGAAGAAT TTAGTAAAAA GGGTTTATAT TATAAACACA ATTGTATATG GAAAAACAAA AATTTTACAA TTGATAATCT TACTAGAGTC ATTAGATTAA GAAGCCCTAC TGAAGGTGTC ACATCATTTG ACGATAAAGT ATATGGTAAT ATCACAGTAA GTAATACACA ATTAGATGAT ATGGTTTTAT TGCGTTCTGA TAACACTCCT ACATATCTCC TATCCGTAGT TGTAGATGAC CATGACATGA ATATTACTCA TATTATTAGA GGAACAGACC ATTTAACAAA TACAGCACGT CAATTATTAA TATATAATGC ATTAGAATGG AATCCTCCTA AATTTGCTCA TATTCCATTG ATACATGATG AAGATGGTAA TAAATTATCT AAAAGACATC AAGCAATTGG CATACATGAA TATAAAAATT TAGGTATATT ACCAGAAGCA ATATCCAATT ACTTGTTAAG AATGGGATGG AGTCATGAAG ATGATGAGAT TATTAGTATG GATCAAGCAA TAAAGTGGTT TTCTATAAAG AATATCGGAC AATCTCCGGC AAGATTAGAT AATAAAAAAC TGGAATTTTT AAACAATCAC TATATCAGTC TTACTGAAGA TGAAGTGATT CTGAATATGA TTATTCCAAT TATAGAAAAA AAGATAGGAT ATATGCTAAA TGAAGTAAAA AAAGGATATT TATTAAAAGG ATTATATGAA CTAAAGAAAA GGACAAAAAA TCTAGTAAAT TTAGCAAACG AATCTTTGTT TTACGTTGAA GATGTACCAA TCTCTATTGA TCAAGAAGCT AGTGCTATTA TAAAAGATTA TAAGCACGTG TTTTCTATAT TATATAATAA TTTATCAAGA ATTTCAGAAA AAGAATGGAA TAACAGCATA CTAACATCAA CAATAAAAAA CATTTCACAA AACTTAGATA TAAAGATTAG TAACATCTAT CATTGTCTGC GTGCATCTAT TGTTGGAAGA ATGAATGCTC CAAGTATTAT TGAAATTATG ATTAATCTTC AACAAGAAGA ATGTCTAAAA CGTATAAAGT ATGCACAAAA TATAGAA

Details for EhchA.01521.a.A1.GE27446

HARVESTED ON:	9/15/2009
SEQUENCED ON:	10/20/2009
EXPECTED MW:	56kDa
OBSERVED MW:	57kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSHNVITRF APSPTGHLHL GGARTALFNW LYAKHNNGKF LLRIEDTDKK RSSKELIXSI INAMSWLKIP YDGEIVLQSK NISRHIEIAN QLILNNKAYY CYCSEEEINK XKEEFSKKGL YYKHNCIWKN KNFTIDNLTR VIRLXSPTEG VTSFDDKVYG NITXSNTQLD DMVLLRSDNT PTXXLSVVVD DHDMXXTHII RXTDHLTNTA RXLXIYNALE WNPPKFAHIP LIHDEDGNKL SKRHQAIGIH EYKNLGILPE AISNYLLRMG WSHEDDEIIS MDQAIKWFSI KNIGQSPARL DNKKLEFLNN HYISLTEDEV ILNMIIPIIE KKIGYMLNEV KKGYLLKGLY ELKKRTKNLV NLANESLFYV EDVPISIDQE ASAIIKDYKH VFSILYNNLS RISEKEWNNS ILTSTIKNIS QNLDIKISNI YHCLRASIVG RMNAPSIIEI MINLQQEECL KRIKYAQNIE

Validated NT Sequence

ttattattct atattttgtg catactttat acgttttaga cattcttctt gttgaagatt aatcataatt tcaataatac ttggagcatt cattcttcca acaatagatg cacgcagaca atgatagatg ttactaatct ttatatctaa gttttgtgaa atgtttttta ttgttgatgt tagtatgctg ttattccatt ctttttctga aattcttgat aaattattat ataatataga aaacacgtgc ttataatctt ttataatagc actagcttct tgatcaatag agattggtac atcttcaacg taaaacaaag attcgtttgc taaatttact agattttttg tccttttctt tagttcatat aatcctttta ataaatatcc tttttttact tcatttagca tatatcctat ctttttttct ataattggaa taatcatatt cagaatcact tcatcttcag taagactgat atagtgattg tttaaaaatt ccagtttttt attatctaat cttgccggag attgtccgat attctttata gaaaaccact ttattgcttg atccatacta ataatctcat catcttcatg actccatccc attcttaaca agtaattgga tattgcttct ggtaatatac ctaaattttt atattcatgt atgccaattg cttgatgtct tttagataat ttattaccat cttcatcatg tatcaatgga atatgagcaa atttaggagg attccattct aatgcattat atattantaa ntgacgtgct gtatttgtta aatggtcngt tnntctaata atatgagtnn nantcatgtc atggtcatct acaactacgg atagganann tgtaggagtg ttatcagaac gcaataaaac catatcatct aattgtgtat tacttanngt gatattacca tatactttat cgtcaaatga tgtgacacct tcagtagggc ttnttaatct aatgactcta gtaagattat caattgtaaa atttttgttt ttccatatac aattgtgttt ataatataaa ccctttttac taaattcttc tttttnttta tttatttctt cctcagaaca ataacagtaa tatgctttat tgtttaatat tagttgattt gcaatttcaa tatgtctaga tatatttttt gattgtaata ctatttcccc atcataagga atttttaacc aagacattgc attaataata gagtngataa gttctttaga tgatcttttt ttatcagtat cttcaatgcg taataaaaat tttccattgt tatgtttagc atatagccag ttgaatagag ctgttcttgc tcctcctaaa tgtaaatgtc cagttggtga aggggcaaat cgagttatta cattatgtga catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccatggta t

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSHNVITRF APSPTGHLHL GGARTALFNW LYAKHNNGKF LLRIEDTDKK RSSKELIDSI INAMSWLKIP YDGEIVLQSK NISRHIEIAN QLILNNKAYY CYCSEEEINK EKEEFSKKGL YYKHNCIWKN KNFTIDNLTR VIRLRSPTEG VTSFDDKVYG NITVSNTQLD DMVLLRSDNT PTYLLSVVVD DHDMNITHII RGTDHLTNTA RQLLIYNALE WNPPKFAHIP LIHDEDGNKL SKRHQAIGIH EYKNLGILPE AISNYLLRMG WSHEDDEIIS MDQAIKWFSI KNIGQSPARL DNKKLEFLNN HYISLTEDEV ILNMIIPIIE KKIGYMLNEV KKGYLLKGLY ELKKRTKNLV NLANESLFYV EDVPISIDQE ASAIIKDYKH VFSILYNNLS RISEKEWNNS ILTSTIKNIS QNLDIKISNI YHCLRASIVG RMNAPSIIEI MINLQQEECL KRIKYAQNIE

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcacataat gtaataactc gatttgcccc ttcaccaact ggacatttac atttaggagg agcaagaaca gctctattca actggctata tgctaaacat aacaatggaa aatttttatt acgcattgaa gatactgata aaaaaagatc atctaaagaa cttatcgact ctattattaa tgcaatgtct tggttaaaaa ttccttatga tggggaaata gtattacaat caaaaaatat atctagacat attgaaattg caaatcaact aatattaaac aataaagcat attactgtta ttgttctgag gaagaaataa ataaagaaaa agaagaattt agtaaaaagg gtttatatta taaacacaat tgtatatgga aaaacaaaaa ttttacaatt gataatctta ctagagtcat tagattaaga agccctactg aaggtgtcac atcatttgac gataaagtat atggtaatat cacagtaagt aatacacaat tagatgatat ggttttattg cgttctgata acactcctac atatctccta tccgtagttg tagatgacca tgacatgaat attactcata ttattagagg aacagaccat ttaacaaata cagcacgtca attattaata tataatgcat tagaatggaa tcctcctaaa tttgctcata ttccattgat acatgatgaa gatggtaata aattatctaa aagacatcaa gcaattggca tacatgaata taaaaattta ggtatattac cagaagcaat atccaattac ttgttaagaa tgggatggag tcatgaagat gatgagatta ttagtatgga tcaagcaata aagtggtttt ctataaagaa tatcggacaa tctccggcaa gattagataa taaaaaactg gaatttttaa acaatcacta tatcagtctt actgaagatg aagtgattct gaatatgatt attccaatta tagaaaaaaa gataggatat atgctaaatg aagtaaaaaa aggatattta ttaaaaggat tatatgaact aaagaaaagg acaaaaaatc tagtaaattt agcaaacgaa tctttgtttt acgttgaaga tgtaccaatc tctattgatc aagaagctag tgctattata aaagattata agcacgtgtt ttctatatta tataataatt tatcaagaat ttcagaaaaa gaatggaata acagcatact aacatcaaca ataaaaaaca tttcacaaaa cttagatata aagattagta acatctatca ttgtctgcgt gcatctattg ttggaagaat gaatgctcca agtattattg aaattatgat taatcttcaa caagaagaat gtctaaaacg tataaagtat gcacaaaata tagaaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for EhchA.01521.a.A1.PS00391

PURIFICATION DATe:	10/29/2009
CONCENTRATION:	29.7mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCACATAAT GTAATAACTC GATTTGCCCC TTCACCAACT GGACATTTAC ATTTAGGAGG AGCAAGAACA GCTCTATTCA ACTGGCTATA TGCTAAACAT AACAATGGAA AATTTTTATT ACGCATTGAA GATACTGATA AAAAAAGATC ATCTAAAGAA CTTATCGACT CTATTATTAA TGCAATGTCT TGGTTAAAAA TTCCTTATGA TGGGGAAATA GTATTACAAT CAAAAAATAT ATCTAGACAT ATTGAAATTG CAAATCAACT AATATTAAAC AATAAAGCAT ATTACTGTTA TTGTTCTGAG GAAGAAATAA ATAAAGAAAA AGAAGAATTT AGTAAAAAGG GTTTATATTA TAAACACAAT TGTATATGGA AAAACAAAAA TTTTACAATT GATAATCTTA CTAGAGTCAT TAGATTAAGA AGCCCTACTG AAGGTGTCAC ATCATTTGAC GATAAAGTAT ATGGTAATAT CACAGTAAGT AATACACAAT TAGATGATAT GGTTTTATTG CGTTCTGATA ACACTCCTAC ATATCTCCTA TCCGTAGTTG TAGATGACCA TGACATGAAT ATTACTCATA TTATTAGAGG AACAGACCAT TTAACAAATA CAGCACGTCA ATTATTAATA TATAATGCAT TAGAATGGAA TCCTCCTAAA TTTGCTCATA TTCCATTGAT ACATGATGAA GATGGTAATA AATTATCTAA AAGACATCAA GCAATTGGCA TACATGAATA TAAAAATTTA GGTATATTAC CAGAAGCAAT ATCCAATTAC TTGTTAAGAA TGGGATGGAG TCATGAAGAT GATGAGATTA TTAGTATGGA TCAAGCAATA AAGTGGTTTT CTATAAAGAA TATCGGACAA TCTCCGGCAA GATTAGATAA TAAAAAACTG GAATTTTTAA ACAATCACTA TATCAGTCTT ACTGAAGATG AAGTGATTCT GAATATGATT ATTCCAATTA TAGAAAAAAA GATAGGATAT ATGCTAAATG AAGTAAAAAA AGGATATTTA TTAAAAGGAT TATATGAACT AAAGAAAAGG ACAAAAAATC TAGTAAATTT AGCAAACGAA TCTTTGTTTT ACGTTGAAGA TGTACCAATC TCTATTGATC AAGAAGCTAG TGCTATTATA AAAGATTATA AGCACGTGTT TTCTATATTA TATAATAATT TATCAAGAAT TTCAGAAAAA GAATGGAATA ACAGCATACT AACATCAACA ATAAAAAACA TTTCACAAAA CTTAGATATA AAGATTAGTA ACATCTATCA TTGTCTGCGT GCATCTATTG TTGGAAGAAT GAATGCTCCA AGTATTATTG AAATTATGAT TAATCTTCAA CAAGAAGAAT GTCTAAAACG TATAAAGTAT GCACAAAATA TAGAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA