EhchA.01454.a

PolyA polymerase/tRNA nucleotidyltransferase family protein

CENTER ID: EhchA.01454.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01454.a.A1.GE30838 Full length( EhchA.01454.a ) 1 401
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01454.a.A1.PW30726 Full length( EhchA.01454.a ) 1 401

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.956
RefSeq: YP_507900.1
UniProt: Q2GF83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYDDFINNEN ILLIINAIKK FQGDIRLVGG CVRDSLLKRQ TIDIDFATTL LPNQTINALT AAHIKAIPTG IKHGTITALV NNTAYEITTL RSDISCDGRH AEVKFTNNWQ QDASRRDFTF NALYCDEKGI VYDYFSGIQD LEKKHLNFIG DPEIRIQEDY LRILRAFRFY ASICSQNKLS DEIVHSCTKY SSYINNLSRE RIRDEFFKLL LCPNLSNTLK IMQKCHVLDK IIPFEVIPDI MSSETLSNTD PLTKLAALLR TNNNNHSLDK IKASLCLSNY SQKTLVSLLN NNLELPLSTT AQHKHINKLG KEIYCNLLRI IHAELNLNYH DLMQYIEYAD QLIIPEFPIS GKDLLNIGYQ PGKNLGITLE KIKDLWENSS YQLTKTQLLD YARGKLLKSK N
NT Sequence
ATGTACGATG ACTTTATAAA TAATGAGAAT ATTCTGTTAA TCATAAATGC AATAAAAAAA TTTCAAGGAG ATATTAGGTT AGTAGGTGGA TGTGTAAGAG ATAGTCTTCT AAAAAGACAG ACCATAGATA TTGATTTTGC AACTACTTTA TTACCCAACC AAACAATAAA TGCTCTTACT GCAGCTCATA TTAAAGCTAT TCCAACAGGT ATAAAACATG GTACAATAAC AGCCTTAGTT AATAATACAG CATATGAAAT TACAACACTA AGATCTGATA TTAGCTGTGA TGGAAGACAT GCTGAAGTAA AATTTACAAA CAATTGGCAG CAAGACGCTT CAAGAAGAGA TTTCACCTTC AATGCTCTAT ATTGTGATGA AAAAGGAATA GTATATGATT ATTTTTCTGG TATCCAAGAT CTAGAAAAAA AACATCTAAA TTTTATTGGA GATCCAGAAA TTAGAATACA AGAAGACTAC CTACGCATAC TTCGAGCATT TAGATTTTAT GCTTCTATAT GTAGTCAAAA CAAATTGAGT GATGAAATAG TGCACTCTTG CACAAAATAT TCATCTTATA TCAATAACCT ATCCAGAGAA CGCATTCGCG ATGAGTTCTT TAAACTTTTA TTATGTCCTA ACTTATCAAA CACATTAAAG ATTATGCAAA AATGCCACGT GCTAGATAAA ATCATTCCCT TTGAAGTCAT ACCAGACATA ATGTCATCTG AGACCTTATC AAACACAGAT CCACTAACAA AATTAGCAGC TCTTTTAAGA ACAAACAATA ACAACCACTC TCTAGATAAA ATTAAAGCTT CTTTATGCTT ATCAAACTAC AGTCAAAAAA CACTTGTGTC ACTATTAAAC AATAATTTAG AACTTCCACT TTCAACTACC GCACAACACA AACACATTAA CAAGCTTGGA AAAGAAATAT ACTGCAATCT ACTGAGAATA ATACATGCAG AATTAAATTT AAATTATCAT GACCTAATGC AATATATAGA GTACGCAGAT CAATTAATTA TTCCTGAATT TCCTATCTCT GGAAAAGATT TACTTAATAT AGGATACCAA CCAGGAAAAA ATCTTGGTAT CACTTTAGAA AAAATCAAAG ATCTATGGGA AAATAGTTCA TATCAACTAA CAAAAACCCA ATTATTAGAT TACGCGAGAG GAAAATTATT AAAAAGTAAG AAT
Details for EhchA.01454.a.A1.GE30838
HARVESTED ON: 11/16/2010
SEQUENCED ON: 11/23/2010
EXPECTED MW: 48kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 42
Validated AA Sequence
MQKCHVLDKI IPFEVIPDIM SSETLSNTDP LTKLAALLRT NNNNHSLDKI KASLCLSNYS QKTLVSLLNN NLELPLSTTA QHKHINKLGK EIYCNLLRII HAELNLNYHD LMQYIEYADQ LIIPEFPISG KDLLNIGYQP GKNLGITLEK IKDLWENSSY QLTKTQLLDY ARGKLLKS
Validated NT Sequence
tactttttaa taattttcct ctcgcgtaat ctaataattg ggtttttgtt agttgatatg aactattttc ccatagatct ttgatttttt ctaaagtgat accaagattt tttcctggtt ggtatcctat attaagtaaa tcttttccag agataggaaa ttcaggaata attaattgat ctgcgtactc tatatattgc attaggtcat gataatttaa atttaattct gcatgtatta ttctcagtag attgcagtat atttcttttc caagcttgtt aatgtgtttg tgttgtgcgg tagttgaaag tggaagttct aaattattgt ttaatagtga cacaagtgtt ttttgactgt agtttgataa gcataaagaa gctttaattt tatctagaga gtggttgtta ttgtttgttc ttaaaagagc tgctaatttt gttagtggat ctgtgtttga taaggtctca gatgacatta tgtctggtat gacttcaaag ggaatgattt tatctagcac gtggcatttt tgcataatct ttaatgtgtt tgataagtta ggacataata aaagtttaaa gaactcatcg cgaatgcgtt ctctggatag gttattgata taagatgaat attttgtgca agagtgcact atttcatcac tcaatttgtt ttgactacat atagaagcat aaaatctaaa tgctcgaagt atgcgtangt agtcttcttg tattctaatt tctggatctc caataaaatt tanatgnttt tttttctaga tcttggntac cagaaaaata atcatatact attccttttt cntcacnata tagagcattg na
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYDDFINNE NILLIINAIK KFQGDIRLVG GCVRDSLLKR QTIDIDFATT LLPNQTINAL TAAHIKAIPT GIKHGTITAL VNNTAYEITT LRSDISCDGR HAEVKFTNNW QQDASRRDFT FNALYCDEKG IVYDYFSGIQ DLEKKHLNFI GDPEIRIQED YLRILRAFRF YASICSQNKL SDEIVHSCTK YSSYINNLSR ERIRDEFFKL LLCPNLSNTL KIMQKCHVLD KIIPFEVIPD IMSSETLSNT DPLTKLAALL RTNNNNHSLD KIKASLCLSN YSQKTLVSLL NNNLELPLST TAQHKHINKL GKEIYCNLLR IIHAELNLNY HDLMQYIEYA DQLIIPEFPI SGKDLLNIGY QPGKNLGITL EKIKDLWENS SYQLTKTQLL DYARGKLLKS KN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtacgatgac tttataaata atgagaatat tctgttaatc ataaatgcaa taaaaaaatt tcaaggagat attaggttag taggtggatg tgtaagagat agtcttctaa aaagacagac catagatatt gattttgcaa ctactttatt acccaaccaa acaataaatg ctcttactgc agctcatatt aaagctattc caacaggtat aaaacatggt acaataacag ccttagttaa taatacagca tatgaaatta caacactaag atctgatatt agctgtgatg gaagacatgc tgaagtaaaa tttacaaaca attggcagca agacgcttca agaagagatt tcaccttcaa tgctctatat tgtgatgaaa aaggaatagt atatgattat ttttctggta tccaagatct agaaaaaaaa catctaaatt ttattggaga tccagaaatt agaatacaag aagactacct acgcatactt cgagcattta gattttatgc ttctatatgt agtcaaaaca aattgagtga tgaaatagtg cactcttgca caaaatattc atcttatatc aataacctat ccagagaacg cattcgcgat gagttcttta aacttttatt atgtcctaac ttatcaaaca cattaaagat tatgcaaaaa tgccacgtgc tagataaaat cattcccttt gaagtcatac cagacataat gtcatctgag accttatcaa acacagatcc actaacaaaa ttagcagctc ttttaagaac aaacaataac aaccactctc tagataaaat taaagcttct ttatgcttat caaactacag tcaaaaaaca cttgtgtcac tattaaacaa taatttagaa cttccacttt caactaccgc acaacacaaa cacattaaca agcttggaaa agaaatatac tgcaatctac tgagaataat acatgcagaa ttaaatttaa attatcatga cctaatgcaa tatatagagt acgcagatca attaattatt cctgaatttc ctatctctgg aaaagattta cttaatatag gataccaacc aggaaaaaat cttggtatca ctttagaaaa aatcaaagat ctatgggaaa atagttcata tcaactaaca aaaacccaat tattagatta cgcgagagga aaattattaa aaagtaagaa ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for EhchA.01454.a.A1.PW30726
PURIFICATION DATe: 3/11/2011
CONCENTRATION: 21.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 42
Protocol Notes
notes unavailable
Validated AA Sequence
MQKCHVLDKI IPFEVIPDIM SSETLSNTDP LTKLAALLRT NNNNHSLDKI KASLCLSNYS QKTLVSLLNN NLELPLSTTA QHKHINKLGK EIYCNLLRII HAELNLNYHD LMQYIEYADQ LIIPEFPISG KDLLNIGYQP GKNLGITLEK IKDLWENSSY QLTKTQLLDY ARGKLLKS
Validated NT Sequence
tactttttaa taattttcct ctcgcgtaat ctaataattg ggtttttgtt agttgatatg aactattttc ccatagatct ttgatttttt ctaaagtgat accaagattt tttcctggtt ggtatcctat attaagtaaa tcttttccag agataggaaa ttcaggaata attaattgat ctgcgtactc tatatattgc attaggtcat gataatttaa atttaattct gcatgtatta ttctcagtag attgcagtat atttcttttc caagcttgtt aatgtgtttg tgttgtgcgg tagttgaaag tggaagttct aaattattgt ttaatagtga cacaagtgtt ttttgactgt agtttgataa gcataaagaa gctttaattt tatctagaga gtggttgtta ttgtttgttc ttaaaagagc tgctaatttt gttagtggat ctgtgtttga taaggtctca gatgacatta tgtctggtat gacttcaaag ggaatgattt tatctagcac gtggcatttt tgcataatct ttaatgtgtt tgataagtta ggacataata aaagtttaaa gaactcatcg cgaatgcgtt ctctggatag gttattgata taagatgaat attttgtgca agagtgcact atttcatcac tcaatttgtt ttgactacat atagaagcat aaaatctaaa tgctcgaagt atgcgtangt agtcttcttg tattctaatt tctggatctc caataaaatt tanatgnttt tttttctaga tcttggntac cagaaaaata atcatatact attccttttt cntcacnata tagagcattg na
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMYDDFINNE NILLIINAIK KFQGDIRLVG GCVRDSLLKR QTIDIDFATT LLPNQTINAL TAAHIKAIPT GIKHGTITAL VNNTAYEITT LRSDISCDGR HAEVKFTNNW QQDASRRDFT FNALYCDEKG IVYDYFSGIQ DLEKKHLNFI GDPEIRIQED YLRILRAFRF YASICSQNKL SDEIVHSCTK YSSYINNLSR ERIRDEFFKL LLCPNLSNTL KIMQKCHVLD KIIPFEVIPD IMSSETLSNT DPLTKLAALL RTNNNNHSLD KIKASLCLSN YSQKTLVSLL NNNLELPLST TAQHKHINKL GKEIYCNLLR IIHAELNLNY HDLMQYIEYA DQLIIPEFPI SGKDLLNIGY QPGKNLGITL EKIKDLWENS SYQLTKTQLL DYARGKLLKS KN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTACGATGAC TTTATAAATA ATGAGAATAT TCTGTTAATC ATAAATGCAA TAAAAAAATT TCAAGGAGAT ATTAGGTTAG TAGGTGGATG TGTAAGAGAT AGTCTTCTAA AAAGACAGAC CATAGATATT GATTTTGCAA CTACTTTATT ACCCAACCAA ACAATAAATG CTCTTACTGC AGCTCATATT AAAGCTATTC CAACAGGTAT AAAACATGGT ACAATAACAG CCTTAGTTAA TAATACAGCA TATGAAATTA CAACACTAAG ATCTGATATT AGCTGTGATG GAAGACATGC TGAAGTAAAA TTTACAAACA ATTGGCAGCA AGACGCTTCA AGAAGAGATT TCACCTTCAA TGCTCTATAT TGTGATGAAA AAGGAATAGT ATATGATTAT TTTTCTGGTA TCCAAGATCT AGAAAAAAAA CATCTAAATT TTATTGGAGA TCCAGAAATT AGAATACAAG AAGACTACCT ACGCATACTT CGAGCATTTA GATTTTATGC TTCTATATGT AGTCAAAACA AATTGAGTGA TGAAATAGTG CACTCTTGCA CAAAATATTC ATCTTATATC AATAACCTAT CCAGAGAACG CATTCGCGAT GAGTTCTTTA AACTTTTATT ATGTCCTAAC TTATCAAACA CATTAAAGAT TATGCAAAAA TGCCACGTGC TAGATAAAAT CATTCCCTTT GAAGTCATAC CAGACATAAT GTCATCTGAG ACCTTATCAA ACACAGATCC ACTAACAAAA TTAGCAGCTC TTTTAAGAAC AAACAATAAC AACCACTCTC TAGATAAAAT TAAAGCTTCT TTATGCTTAT CAAACTACAG TCAAAAAACA CTTGTGTCAC TATTAAACAA TAATTTAGAA CTTCCACTTT CAACTACCGC ACAACACAAA CACATTAACA AGCTTGGAAA AGAAATATAC TGCAATCTAC TGAGAATAAT ACATGCAGAA TTAAATTTAA ATTATCATGA CCTAATGCAA TATATAGAGT ACGCAGATCA ATTAATTATT CCTGAATTTC CTATCTCTGG AAAAGATTTA CTTAATATAG GATACCAACC AGGAAAAAAT CTTGGTATCA CTTTAGAAAA AATCAAAGAT CTATGGGAAA ATAGTTCATA TCAACTAACA AAAACCCAAT TATTAGATTA CGCGAGAGGA AAATTATTAA AAAGTAAGAA TTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA