EhchA.01376.a

enoyl-(acyl-carrier-protein) reductase

CENTER ID: EhchA.01376.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01376.a.A1.GE27449 Full length( EhchA.01376.a ) 1 266
EhchA.01376.a.A1.GE27449 Full length( EhchA.01376.a ) 1 266
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.708
RefSeq: YP_507608.1
UniProt: Q2GG25

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKGKKGLVIG IANDKSLAWG IAKTLAMHGA DIAVTYLSDV FKKRIDPLAE SIGVKLVLQC DVSKEETVDN MFAQLSDEWE SMDFLVHAVA FSDKNELKGR YIDTSLNNFL TSMHISCYSL TYLAKKVEKM MCNGGSILTL SYYGAQKVMP HYNVMGVCKA ALEASVKYLA VDLGSQNIRV NAISAGPVRT LASSGIGDFH YILTWNKYNA PLRRNTTIDD VGGAALYLLS DLGKGTTGEI LYVDGGYHAI GMKAIDAPDI NYIEAK
NT Sequence
ATGAAAGGAA AAAAAGGATT AGTCATTGGA ATTGCTAATG ATAAATCTTT AGCATGGGGG ATTGCTAAAA CTTTAGCTAT GCATGGGGCA GATATAGCAG TGACTTATTT ATCCGACGTT TTTAAGAAAA GGATAGATCC TTTAGCGGAA TCTATAGGTG TAAAGTTGGT CTTGCAATGT GATGTATCTA AAGAAGAGAC TGTAGATAAT ATGTTTGCAC AGCTTAGTGA TGAATGGGAA AGTATGGATT TTTTAGTACA TGCTGTTGCT TTTTCTGATA AAAATGAGCT GAAAGGTAGG TATATTGATA CGTCATTGAA TAATTTTTTG ACATCTATGC ATATTTCATG TTACTCGCTT ACTTACCTTG CTAAAAAAGT AGAGAAGATG ATGTGTAATG GAGGTAGTAT ATTAACATTA TCTTATTATG GTGCTCAAAA AGTGATGCCT CACTATAATG TGATGGGAGT ATGTAAAGCA GCTTTAGAAG CAAGTGTAAA GTATCTGGCT GTTGATTTAG GTTCTCAGAA TATTAGGGTT AATGCTATTT CTGCAGGTCC AGTAAGAACT TTAGCATCTT CTGGGATAGG AGATTTCCAT TACATATTGA CATGGAATAA ATATAATGCA CCTTTGAGGC GTAATACTAC AATAGACGAT GTGGGTGGAG CAGCACTTTA TTTATTAAGT GATTTAGGTA AAGGTACTAC AGGAGAAATT TTATATGTTG ATGGTGGATA TCATGCTATA GGTATGAAAG CAATAGATGC TCCTGATATT AATTATATTG AAGCCAAA
Details for EhchA.01376.a.A1.GE27449
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 31kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKGKKGLVI GIANDKSLAW GIAKTLAMHG ADIAVTYLSD VFKKRIDPLA ESIGVKLVLQ CDVSKEETVD NMFAQLSDEW ESMDFLVHAV AFSDKNELKG RYIDTSLNNF LTSMHISCYS LTYLAKKVEK MMCNGGSILT LSYYGAQKVM PHYNVMGVCK AALEASVKYL AVDLGSQNIR VNAISAGPVR TLASSGIGDF HYILTWNKYN APLRRNTTID DVGGAALYLL SDLGKGTTGE ILYVDGGYHA IGMKAIDAPD INYIEAK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaag gaaaaaaagg attagtcatt ggaattgcta atgataaatc tttagcatgg gggattgcta aaactttagc tatgcatggg gcagatatag cagtgactta tttatccgac gtttttaaga aaaggataga tcctttagcg gaatctatag gtgtaaagtt ggtcttgcaa tgtgatgtat ctaaagaaga gactgtagat aatatgtttg cacagcttag tgatgaatgg gaaagtatgg attttttagt acatgctgtt gctttttctg ataaaaatga gctgaaaggt aggtatattg atacgtcatt gaataatttt ttgacatcta tgcatatttc atgttactcg cttacttacc ttgctaaaaa agtagagaag atgatgtgta atggaggtag tatattaaca ttatcttatt atggtgctca aaaagtgatg cctcactata atgtgatggg agtatgtaaa gcagctttag aagcaagtgt aaagtatctg gctgttgatt taggttctca gaatattagg gttaatgcta tttctgcagg tccagtaaga actttagcat cttctgggat aggagatttc cattacatat tgacatggaa taaatataat gcacctttga ggcgtaatac tacaatagac gatgtgggtg gagcagcact ttatttatta agtgatttag gtaaaggtac tacaggagaa attttatatg ttgatggtgg atatcatgct ataggtatga aagcaataga tgctcctgat attaattata ttgaagccaa ataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKGKKGLVI GIANDKSLAW GIAKTLAMHG ADIAVTYLSD VFKKRIDPLA ESIGVKLVLQ CDVSKEETVD NMFAQLSDEW ESMDFLVHAV AFSDKNELKG RYIDTSLNNF LTSMHISCYS LTYLAKKVEK MMCNGGSILT LSYYGAQKVM PHYNVMGVCK AALEASVKYL AVDLGSQNIR VNAISAGPVR TLASSGIGDF HYILTWNKYN APLRRNTTID DVGGAALYLL SDLGKGTTGE ILYVDGGYHA IGMKAIDAPD INYIEAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaggaaaa aaaggattag tcattggaat tgctaatgat aaatctttag catgggggat tgctaaaact ttagctatgc atggggcaga tatagcagtg acttatttat ccgacgtttt taagaaaagg atagatcctt tagcggaatc tataggtgta aagttggtct tgcaatgtga tgtatctaaa gaagagactg tagataatat gtttgcacag cttagtgatg aatgggaaag tatggatttt ttagtacatg ctgttgcttt ttctgataaa aatgagctga aaggtaggta tattgatacg tcattgaata attttttgac atctatgcat atttcatgtt actcgcttac ttaccttgct aaaaaagtag agaagatgat gtgtaatgga ggtagtatat taacattatc ttattatggt gctcaaaaag tgatgcctca ctataatgtg atgggagtat gtaaagcagc tttagaagca agtgtaaagt atctggctgt tgatttaggt tctcagaata ttagggttaa tgctatttct gcaggtccag taagaacttt agcatcttct gggataggag atttccatta catattgaca tggaataaat ataatgcacc tttgaggcgt aatactacaa tagacgatgt gggtggagca gcactttatt tattaagtga tttaggtaaa ggtactacag gagaaatttt atatgttgat ggtggatatc atgctatagg tatgaaagca atagatgctc ctgatattaa ttatattgaa gccaaaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for EhchA.01376.a.A1.GE27449
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 31kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKGKKGLVI GIANDKSLAW GIAKTLAMHG ADIAVTYLSD VFKKRIDPLA ESIGVKLVLQ CDVSKEETVD NMFAQLSDEW ESMDFLVHAV AFSDKNELKG RYIDTSLNNF LTSMHISCYS LTYLAKKVEK MMCNGGSILT LSYYGAQKVM PHYNVMGVCK AALEASVKYL AVDLGSQNIR VNAISAGPVR TLASSGIGDF HYILTWNKYN APLRRNTTID DVGGAALYLL SDLGKGTTGE ILYVDGGYHA IGMKAIDAPD INYIEAK
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaag gaaaaaaagg attagtcatt ggaattgcta atgataaatc tttagcatgg gggattgcta aaactttagc tatgcatggg gcagatatag cagtgactta tttatccgac gtttttaaga aaaggataga tcctttagcg gaatctatag gtgtaaagtt ggtcttgcaa tgtgatgtat ctaaagaaga gactgtagat aatatgtttg cacagcttag tgatgaatgg gaaagtatgg attttttagt acatgctgtt gctttttctg ataaaaatga gctgaaaggt aggtatattg atacgtcatt gaataatttt ttgacatcta tgcatatttc atgttactcg cttacttacc ttgctaaaaa agtagagaag atgatgtgta atggaggtag tatattaaca ttatcttatt atggtgctca aaaagtgatg cctcactata atgtgatggg agtatgtaaa gcagctttag aagcaagtgt aaagtatctg gctgttgatt taggttctca gaatattagg gttaatgcta tttctgcagg tccagtaaga actttagcat cttctgggat aggagatttc cattacatat tgacatggaa taaatataat gcacctttga ggcgtaatac tacaatagac gatgtgggtg gagcagcact ttatttatta agtgatttag gtaaaggtac tacaggagaa attttatatg ttgatggtgg atatcatgct ataggtatga aagcaataga tgctcctgat attaattata ttgaagccaa ataataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKGKKGLVI GIANDKSLAW GIAKTLAMHG ADIAVTYLSD VFKKRIDPLA ESIGVKLVLQ CDVSKEETVD NMFAQLSDEW ESMDFLVHAV AFSDKNELKG RYIDTSLNNF LTSMHISCYS LTYLAKKVEK MMCNGGSILT LSYYGAQKVM PHYNVMGVCK AALEASVKYL AVDLGSQNIR VNAISAGPVR TLASSGIGDF HYILTWNKYN APLRRNTTID DVGGAALYLL SDLGKGTTGE ILYVDGGYHA IGMKAIDAPD INYIEAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaggaaaa aaaggattag tcattggaat tgctaatgat aaatctttag catgggggat tgctaaaact ttagctatgc atggggcaga tatagcagtg acttatttat ccgacgtttt taagaaaagg atagatcctt tagcggaatc tataggtgta aagttggtct tgcaatgtga tgtatctaaa gaagagactg tagataatat gtttgcacag cttagtgatg aatgggaaag tatggatttt ttagtacatg ctgttgcttt ttctgataaa aatgagctga aaggtaggta tattgatacg tcattgaata attttttgac atctatgcat atttcatgtt actcgcttac ttaccttgct aaaaaagtag agaagatgat gtgtaatgga ggtagtatat taacattatc ttattatggt gctcaaaaag tgatgcctca ctataatgtg atgggagtat gtaaagcagc tttagaagca agtgtaaagt atctggctgt tgatttaggt tctcagaata ttagggttaa tgctatttct gcaggtccag taagaacttt agcatcttct gggataggag atttccatta catattgaca tggaataaat ataatgcacc tttgaggcgt aatactacaa tagacgatgt gggtggagca gcactttatt tattaagtga tttaggtaaa ggtactacag gagaaatttt atatgttgat ggtggatatc atgctatagg tatgaaagca atagatgctc ctgatattaa ttatattgaa gccaaaaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa