SSGCID: Target Page

EhchA.01357.a

putative translaldolase

CENTER ID:	EhchA.01357.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01357.a.A1.GE27447	Full length( EhchA.01357.a )		1	220

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.500
RefSeq:	YP_507386.1
UniProt:	Q2GGP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKIFLDTIDI DFLKEFSVTG LIDGITTNPL LLSKSNLECY DLIVKICSLI TGPVSIEVIS TSYKDMIKEG LEISRIAGNV VVKLPLTYDG LIACKVLSCE HNVKVNVTLC FSVSQAILAA KAGAYFISPF VGRIDDIGQP GMQLIKDIRD VYSNYNAFNT EILVASVRNI THVIQSAKIG ADIVTIPPSI FKQMFMHPLT NKGLEDFLQN WNASGKKIFS

NT Sequence

ATGAAAATAT TCCTTGATAC CATAGATATA GATTTTCTAA AAGAGTTTAG TGTTACTGGT TTGATAGATG GTATTACAAC TAATCCATTG TTGTTATCAA AATCAAATTT GGAATGTTAT GATTTGATTG TAAAGATTTG TTCACTGATA ACAGGTCCTG TTAGTATTGA AGTGATATCT ACATCTTACA AGGATATGAT TAAAGAAGGG TTGGAAATAT CTAGAATTGC TGGTAACGTT GTAGTGAAGC TTCCTTTGAC TTATGATGGA TTAATTGCAT GCAAAGTTTT ATCTTGTGAA CATAATGTAA AGGTTAATGT AACTCTTTGT TTTTCTGTAT CTCAGGCAAT TTTAGCAGCA AAGGCTGGTG CTTATTTTAT ATCTCCTTTT GTAGGAAGAA TAGATGATAT TGGACAACCG GGTATGCAAT TAATTAAGGA TATACGTGAT GTTTATTCTA ATTATAATGC ATTTAATACT GAGATTCTTG TAGCATCTGT ACGTAATATT ACGCATGTTA TACAATCTGC AAAGATAGGT GCTGATATAG TTACGATTCC TCCATCTATT TTTAAGCAAA TGTTTATGCA TCCTCTTACT AATAAAGGTC TTGAAGATTT TTTACAAAAC TGGAATGCAT CTGGAAAAAA AATATTTTCT

Details for EhchA.01357.a.A1.GE27447

HARVESTED ON:	9/15/2009
SEQUENCED ON:	9/30/2009
EXPECTED MW:	26kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKIFLDTID IDFLKEFSVT GLIDGITTNP LLLSKSNLEC YDLIVKICSL ITGPVSIEVI STSYKDMIKE GLEISRIAGN VVVKLPLTYD GLIACKVLSC EHNVKVNVTL CFSVSQAILA AKAGAYFISP FVGRIDDIGQ PGMQLIKDIR DVYSNYNAFN TEILVASVRN ITHVIQSAKI GADIVTIPPS IFKQMFMHPL TNKGLEDFLQ NWNASGKKIF S

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaaa tattccttga taccatagat atagattttc taaaagagtt tagtgttact ggtttgatag atggtattac aactaatcca ttgttgttat caaaatcaaa tttggaatgt tatgatttga ttgtaaagat ttgttcactg ataacaggtc ctgttagtat tgaagtgata tctacatctt acaaggatat gattaaagaa gggttggaaa tatctagaat tgctggtaac gttgtagtga agcttccttt gacttatgat ggattaattg catgcaaagt tttatcttgt gaacataatg taaaggttaa tgtaactctt tgtttttctg tatctcaggc aattttagca gcaaaggctg gtgcttattt tatatctcct tttgtaggaa gaatagatga tattggacaa ccgggtatgc aattaattaa ggatatacgt gatgtttatt ctaattataa tgcatttaat actgagattc ttgtagcatc tgtacgtaat attacgcatg ttatacaatc tgcaaagata ggtgctgata tagttacgat tcctccatct atttttaagc aaatgtttat gcatcctctt actaataaag gtcttgaaga ttttttacaa aactggaatg catctggaaa aaaaatattt tcttaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaatattc cttgatacca tagatataga ttttctaaaa gagtttagtg ttactggttt gatagatggt attacaacta atccattgtt gttatcaaaa tcaaatttgg aatgttatga tttgattgta aagatttgtt cactgataac aggtcctgtt agtattgaag tgatatctac atcttacaag gatatgatta aagaagggtt ggaaatatct agaattgctg gtaacgttgt agtgaagctt cctttgactt atgatggatt aattgcatgc aaagttttat cttgtgaaca taatgtaaag gttaatgtaa ctctttgttt ttctgtatct caggcaattt tagcagcaaa ggctggtgct tattttatat ctccttttgt aggaagaata gatgatattg gacaaccggg tatgcaatta attaaggata tacgtgatgt ttattctaat tataatgcat ttaatactga gattcttgta gcatctgtac gtaatattac gcatgttata caatctgcaa agataggtgc tgatatagtt acgattcctc catctatttt taagcaaatg tttatgcatc ctcttactaa taaaggtctt gaagattttt tacaaaactg gaatgcatct ggaaaaaaaa tattttctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa