EhchA.01355.a

5-aminolevulinate synthase

CENTER ID: EhchA.01355.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01355.a.A1.GE27432 Full length( EhchA.01355.a ) 1 401
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01355.a.A1.PW27425 Full length( EhchA.01355.a ) 1 401
External Resources
RESOURCE REFERENCE ID
OrthoMCL: OG5_126783
PATRIC ID: fig|205920.11.peg.78
RefSeq: YP_506921.1
UniProt: Q2GI12
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIDYEEIFCS KIKRIKDEGR YREFTGFSRI PGQFPYAIEC DVNNVVTLWC SNDYLGMGQN EHMILAIKNY SSSVGAGGTR NISGTTKEII ELEKSLADLH KKPAALTFVC GYIANQTTIS TVLSVIPDIV VFSDEKNHSS MIEGIRSTNR AKHIFRHNDL NHLETLLKSV DISVPKIIIF ESLYSMDGDI APIAKICDLA DKYNAITYLD EVHAVGMYGS RGGGISEQEN ISDRVTIIQG TLSKAFGVMG GYITGSKNVV DVVRSFAPGF IFTTALSPLI ASSARISVEH LKNSSIEREK QREVVNKVKE SFSKAGIDFV KTDTHIIPVI IGDSVACTEI SRVLLKEYRI YIQSINYPTV PVGTERLRIT PTPYHTDEMI DKLTQALVDV LCRFKIMNKQ N
NT Sequence
ATGATAGATT ATGAAGAGAT ATTTTGTAGC AAGATTAAGC GTATAAAAGA TGAAGGTCGA TATAGAGAAT TTACAGGATT CTCACGTATT CCTGGTCAGT TTCCTTATGC TATAGAATGT GATGTAAATA ACGTAGTTAC TCTATGGTGT AGCAATGATT ATTTGGGTAT GGGACAAAAT GAACATATGA TTCTTGCTAT CAAAAATTAT AGTAGTAGTG TAGGTGCTGG TGGTACACGG AATATCTCTG GCACTACAAA GGAGATTATT GAGCTTGAAA AATCATTAGC AGATTTACAT AAAAAACCAG CTGCTCTGAC TTTTGTGTGT GGCTATATTG CAAATCAGAC TACTATTAGT ACAGTGCTTT CTGTAATACC AGATATTGTA GTTTTTTCAG ATGAGAAAAA TCATTCTTCT ATGATAGAAG GTATTAGATC TACTAATAGA GCAAAGCATA TATTTAGGCA CAATGATCTT AATCACCTTG AGACTTTGTT AAAATCTGTT GATATATCTG TACCTAAGAT CATAATATTT GAATCCTTGT ATTCTATGGA TGGAGATATA GCTCCAATTG CAAAAATTTG TGATTTAGCT GATAAATATA ATGCAATAAC TTATTTAGAT GAAGTACATG CAGTTGGTAT GTATGGAAGT CGTGGAGGAG GTATATCAGA ACAAGAAAAT ATATCTGATA GAGTGACAAT TATTCAAGGA ACTCTTTCCA AAGCTTTTGG AGTTATGGGT GGATATATTA CTGGATCAAA AAATGTGGTA GATGTTGTCA GAAGCTTTGC TCCTGGTTTT ATTTTTACTA CAGCATTATC ACCTCTTATT GCATCATCTG CTAGAATAAG TGTTGAGCAT TTAAAGAATA GTTCTATTGA GAGAGAAAAG CAACGTGAGG TTGTTAACAA AGTAAAAGAA TCTTTTTCTA AGGCTGGTAT TGACTTTGTC AAAACGGATA CACATATAAT TCCTGTAATT ATAGGTGATT CAGTGGCTTG TACAGAGATT TCACGAGTGT TACTTAAGGA ATATAGGATA TATATACAAT CTATTAATTA TCCTACTGTG CCTGTAGGTA CAGAGAGATT AAGGATTACT CCAACACCTT ATCATACAGA TGAAATGATA GATAAATTAA CCCAAGCGTT GGTTGATGTT TTGTGTAGGT TTAAAATAAT GAATAAGCAA AAT
Details for EhchA.01355.a.A1.GE27432
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 46kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 41
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIDYEEIFC SKIKRIKDEG RYREFTGFSR IPGQFPYAIE CDVNNVVTLW CSNDYLGMGQ NEHMILAIKN YSSSVGAGGT RNISGTTKEI IELEKSLADL HKKPAALTFV CGYIANXTTI STVLSVIPDI VVFSDEKNHS SMIEGIRSTX XSKAYI
Validated NT Sequence
cttattcatt attttaaacc tacacaaaac atcaaccaac gcttgggtta atttatctat catttcatct gtatgataag gtgttggagt aatccttaat ctctctgtac ctacaggcac agtaggataa ttaatagatt gtatatatat cctatattcc ttaagtaaca ctcgtgaaat ctctgtacaa gccactgaat cacctataat tacaggaatt atatgtgtat ccgttttgac aaagtcaata ccagccttag aaaaagattc ttttactttg ttaacaacct cacgttgctt ttctctctca atagaactat tctttaaatg ctcaacactt attctagcag atgatgcaat aagaggtgat aatgctgtag taaaaataaa accaggagca aagcttctga caacatctac cacatttttt gatccagtaa tatatccacc cataactcca aaagctttgg aaagagttcc ttgaataatt gtcactctat cagatatatt ttcttgttct gatatacctc ctccacgact tccatacata ccaactgcat gtacttcatc taaataagtt attgcattat atttatcagc taaatcacaa atttttgcaa ttggagctat atctccatcc atagaataca aggattcaaa tattatgatc ttaggtacag atatatcaac agattttaac aaagtctcaa ggtgattaag atcattgtgc ctaaatatat gctttgctcn antnagtaga tctaatacct tctatcatag aagaatgatt tttctcatct gaaaaaacta caatatctgg tattacagaa agcactgtac taatagtagt ntgatttgca atatagccac acacaaaagt cagagcagct ggttttttat gtaaatctgc taatgatttt tcaagctcaa taatctcctt tgtagtgcca gagatattcc gtgtaccacc agcacctaca ctactactat aatttttgat agcaagaatc atatgttcat tttgtcccat acccaaataa tcattgctac accatagagt aactacgtta tttacatcac attctatagc ataaggaaac tgaccaggaa tacgtgagaa tcctgtaaat tctctatatc gaccttcatc ttttatacgc ttaatcttgc tacaaaatat ctcttcataa tctatcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca tggtat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIDYEEIFC SKIKRIKDEG RYREFTGFSR IPGQFPYAIE CDVNNVVTLW CSNDYLGMGQ NEHMILAIKN YSSSVGAGGT RNISGTTKEI IELEKSLADL HKKPAALTFV CGYIANQTTI STVLSVIPDI VVFSDEKNHS SMIEGIRSTN RAKHIFRHND LNHLETLLKS VDISVPKIII FESLYSMDGD IAPIAKICDL ADKYNAITYL DEVHAVGMYG SRGGGISEQE NISDRVTIIQ GTLSKAFGVM GGYITGSKNV VDVVRSFAPG FIFTTALSPL IASSARISVE HLKNSSIERE KQREVVNKVK ESFSKAGIDF VKTDTHIIPV IIGDSVACTE ISRVLLKEYR IYIQSINYPT VPVGTERLRI TPTPYHTDEM IDKLTQALVD VLCRFKIMNK QN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatagattat gaagagatat tttgtagcaa gattaagcgt ataaaagatg aaggtcgata tagagaattt acaggattct cacgtattcc tggtcagttt ccttatgcta tagaatgtga tgtaaataac gtagttactc tatggtgtag caatgattat ttgggtatgg gacaaaatga acatatgatt cttgctatca aaaattatag tagtagtgta ggtgctggtg gtacacggaa tatctctggc actacaaagg agattattga gcttgaaaaa tcattagcag atttacataa aaaaccagct gctctgactt ttgtgtgtgg ctatattgca aatcagacta ctattagtac agtgctttct gtaataccag atattgtagt tttttcagat gagaaaaatc attcttctat gatagaaggt attagatcta ctaatagagc aaagcatata tttaggcaca atgatcttaa tcaccttgag actttgttaa aatctgttga tatatctgta cctaagatca taatatttga atccttgtat tctatggatg gagatatagc tccaattgca aaaatttgtg atttagctga taaatataat gcaataactt atttagatga agtacatgca gttggtatgt atggaagtcg tggaggaggt atatcagaac aagaaaatat atctgataga gtgacaatta ttcaaggaac tctttccaaa gcttttggag ttatgggtgg atatattact ggatcaaaaa atgtggtaga tgttgtcaga agctttgctc ctggttttat ttttactaca gcattatcac ctcttattgc atcatctgct agaataagtg ttgagcattt aaagaatagt tctattgaga gagaaaagca acgtgaggtt gttaacaaag taaaagaatc tttttctaag gctggtattg actttgtcaa aacggataca catataattc ctgtaattat aggtgattca gtggcttgta cagagatttc acgagtgtta cttaaggaat ataggatata tatacaatct attaattatc ctactgtgcc tgtaggtaca gagagattaa ggattactcc aacaccttat catacagatg aaatgataga taaattaacc caagcgttgg ttgatgtttt gtgtaggttt aaaataatga ataagcaaaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for EhchA.01355.a.A1.PW27425
PURIFICATION DATe: 10/26/2009
CONCENTRATION: 28.03mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 100µl
PERCENT IDENTITY: 97
PERCENT COVERAGE: 41
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMIDYEEIFC SKIKRIKDEG RYREFTGFSR IPGQFPYAIE CDVNNVVTLW CSNDYLGMGQ NEHMILAIKN YSSSVGAGGT RNISGTTKEI IELEKSLADL HKKPAALTFV CGYIANXTTI STVLSVIPDI VVFSDEKNHS SMIEGIRSTX XSKAYI
Validated NT Sequence
cttattcatt attttaaacc tacacaaaac atcaaccaac gcttgggtta atttatctat catttcatct gtatgataag gtgttggagt aatccttaat ctctctgtac ctacaggcac agtaggataa ttaatagatt gtatatatat cctatattcc ttaagtaaca ctcgtgaaat ctctgtacaa gccactgaat cacctataat tacaggaatt atatgtgtat ccgttttgac aaagtcaata ccagccttag aaaaagattc ttttactttg ttaacaacct cacgttgctt ttctctctca atagaactat tctttaaatg ctcaacactt attctagcag atgatgcaat aagaggtgat aatgctgtag taaaaataaa accaggagca aagcttctga caacatctac cacatttttt gatccagtaa tatatccacc cataactcca aaagctttgg aaagagttcc ttgaataatt gtcactctat cagatatatt ttcttgttct gatatacctc ctccacgact tccatacata ccaactgcat gtacttcatc taaataagtt attgcattat atttatcagc taaatcacaa atttttgcaa ttggagctat atctccatcc atagaataca aggattcaaa tattatgatc ttaggtacag atatatcaac agattttaac aaagtctcaa ggtgattaag atcattgtgc ctaaatatat gctttgctcn antnagtaga tctaatacct tctatcatag aagaatgatt tttctcatct gaaaaaacta caatatctgg tattacagaa agcactgtac taatagtagt ntgatttgca atatagccac acacaaaagt cagagcagct ggttttttat gtaaatctgc taatgatttt tcaagctcaa taatctcctt tgtagtgcca gagatattcc gtgtaccacc agcacctaca ctactactat aatttttgat agcaagaatc atatgttcat tttgtcccat acccaaataa tcattgctac accatagagt aactacgtta tttacatcac attctatagc ataaggaaac tgaccaggaa tacgtgagaa tcctgtaaat tctctatatc gaccttcatc ttttatacgc ttaatcttgc tacaaaatat ctcttcataa tctatcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca tggtat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMIDYEEIFC SKIKRIKDEG RYREFTGFSR IPGQFPYAIE CDVNNVVTLW CSNDYLGMGQ NEHMILAIKN YSSSVGAGGT RNISGTTKEI IELEKSLADL HKKPAALTFV CGYIANQTTI STVLSVIPDI VVFSDEKNHS SMIEGIRSTN RAKHIFRHND LNHLETLLKS VDISVPKIII FESLYSMDGD IAPIAKICDL ADKYNAITYL DEVHAVGMYG SRGGGISEQE NISDRVTIIQ GTLSKAFGVM GGYITGSKNV VDVVRSFAPG FIFTTALSPL IASSARISVE HLKNSSIERE KQREVVNKVK ESFSKAGIDF VKTDTHIIPV IIGDSVACTE ISRVLLKEYR IYIQSINYPT VPVGTERLRI TPTPYHTDEM IDKLTQALVD VLCRFKIMNK QN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATAGATTAT GAAGAGATAT TTTGTAGCAA GATTAAGCGT ATAAAAGATG AAGGTCGATA TAGAGAATTT ACAGGATTCT CACGTATTCC TGGTCAGTTT CCTTATGCTA TAGAATGTGA TGTAAATAAC GTAGTTACTC TATGGTGTAG CAATGATTAT TTGGGTATGG GACAAAATGA ACATATGATT CTTGCTATCA AAAATTATAG TAGTAGTGTA GGTGCTGGTG GTACACGGAA TATCTCTGGC ACTACAAAGG AGATTATTGA GCTTGAAAAA TCATTAGCAG ATTTACATAA AAAACCAGCT GCTCTGACTT TTGTGTGTGG CTATATTGCA AATCAGACTA CTATTAGTAC AGTGCTTTCT GTAATACCAG ATATTGTAGT TTTTTCAGAT GAGAAAAATC ATTCTTCTAT GATAGAAGGT ATTAGATCTA CTAATAGAGC AAAGCATATA TTTAGGCACA ATGATCTTAA TCACCTTGAG ACTTTGTTAA AATCTGTTGA TATATCTGTA CCTAAGATCA TAATATTTGA ATCCTTGTAT TCTATGGATG GAGATATAGC TCCAATTGCA AAAATTTGTG ATTTAGCTGA TAAATATAAT GCAATAACTT ATTTAGATGA AGTACATGCA GTTGGTATGT ATGGAAGTCG TGGAGGAGGT ATATCAGAAC AAGAAAATAT ATCTGATAGA GTGACAATTA TTCAAGGAAC TCTTTCCAAA GCTTTTGGAG TTATGGGTGG ATATATTACT GGATCAAAAA ATGTGGTAGA TGTTGTCAGA AGCTTTGCTC CTGGTTTTAT TTTTACTACA GCATTATCAC CTCTTATTGC ATCATCTGCT AGAATAAGTG TTGAGCATTT AAAGAATAGT TCTATTGAGA GAGAAAAGCA ACGTGAGGTT GTTAACAAAG TAAAAGAATC TTTTTCTAAG GCTGGTATTG ACTTTGTCAA AACGGATACA CATATAATTC CTGTAATTAT AGGTGATTCA GTGGCTTGTA CAGAGATTTC ACGAGTGTTA CTTAAGGAAT ATAGGATATA TATACAATCT ATTAATTATC CTACTGTGCC TGTAGGTACA GAGAGATTAA GGATTACTCC AACACCTTAT CATACAGATG AAATGATAGA TAAATTAACC CAAGCGTTGG TTGATGTTTT GTGTAGGTTT AAAATAATGA ATAAGCAAAA TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA