EhchA.01333.a

acetylglutamate kinase

CENTER ID: EhchA.01333.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01333.a.A1.GE27428 Full length( EhchA.01333.a ) 1 321
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01333.a.A1.PW27421 Full length( EhchA.01333.a ) 1 321

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.515
RefSeq: YP_507405.1
UniProt: Q2GGM8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLHNVLKNN NKEDVVSNIE QFGGNADWFN AARVLSESLP YIQQFSGETF IIKYGGAAMK DRKLAENFAH DIVLLKQLGI NPIVVHGGGS KINEFLEKIN KKSTFVNGLR VTDAETLEIV EMVLCGLVNK DITQLINKAG GNAIGLCGKD ANLIEAKKVC YTYKENQSNN VEKILDMGFV GEPHEVNTDL LFFMEESDFI PVIAPVCSGE NDLTYNVNAD LVAGALANAL AAAKLIILTN VPGVTDVNGN LLSEISVSNA ESLIEQGIAN AGMIPKLQTC IKVVKEGYGS AHIIDGRIPH VLLLELFTIH GTGTMVLNTD I
NT Sequence
ATGAAATTGC ACAATGTATT AAAAAATAAT AATAAAGAAG ATGTTGTATC AAACATTGAG CAATTTGGTG GAAATGCTGA TTGGTTTAAC GCAGCAAGAG TTTTATCAGA ATCCTTACCG TATATACAGC AGTTTTCTGG TGAGACATTT ATCATTAAAT ATGGTGGTGC TGCTATGAAG GATAGAAAAT TAGCAGAAAA TTTTGCTCAC GATATTGTAT TGTTAAAACA ATTAGGTATT AATCCAATAG TTGTGCACGG TGGCGGCAGC AAAATCAACG AATTTCTAGA GAAGATAAAT AAGAAAAGTA CATTTGTTAA TGGATTAAGA GTTACTGATG CTGAAACTTT GGAAATCGTA GAAATGGTAT TATGTGGATT AGTCAATAAA GATATTACAC AACTAATAAA CAAAGCTGGA GGTAATGCTA TTGGATTATG TGGTAAAGAT GCTAATTTAA TAGAAGCAAA AAAAGTTTGT TATACTTATA AGGAAAATCA GTCAAATAAT GTTGAAAAAA TACTAGACAT GGGTTTTGTT GGAGAACCTC ATGAGGTGAA TACAGACTTA TTATTTTTTA TGGAAGAATC TGATTTTATT CCAGTAATAG CTCCTGTATG TAGTGGTGAA AATGATCTTA CTTATAACGT AAATGCAGAT TTAGTAGCTG GTGCATTAGC AAATGCCCTT GCTGCTGCAA AATTGATTAT TTTAACGAAC GTACCTGGAG TTACTGATGT TAATGGTAAC TTGTTATCTG AAATATCAGT AAGTAATGCA GAAAGTCTAA TAGAACAAGG TATAGCAAAT GCAGGTATGA TTCCTAAGTT ACAAACTTGC ATTAAAGTAG TAAAAGAAGG TTATGGATCA GCTCACATAA TAGACGGTAG AATACCACAC GTATTGTTGT TAGAATTGTT TACCATACAT GGAACTGGTA CTATGGTATT AAATACAGAT ATA
Details for EhchA.01333.a.A1.GE27428
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMKLHNVL KNNNKEDVVS NIEQFGGNAD WFNAARVLSE SLPYIQQFSG ETFIIKYGGA AMKDRKLAEN FAHDIVLLKQ LGINPIVVHG GGSKINEFLE KINKKSTFVN GLRVTDAETL EIVEMVLCGL VNKDITQLIN KAGGNAIGLC GKDANLIEAK KVCYTYKENQ SNNVEKILDM GFVGEPHEVN TDLLFFMEES DFIPVIAPVC SGENDLTYNV NADLVAGALA NALAAAKLII LTNVPGVTDV NGNLLSEISV SNAESLIEQG IANAGMIPKL QTCIKVVKEG YGSAHIIDGR IPHVLLLELF TIHGTGTMVL NT
Validated NT Sequence
ctgtatttaa taccatagta ccagttccat gtatggtaaa caattctaac aacaatacgt gtggtattct accgtctatt atgtgagctg atccataacc ttcttttact actttaatgc aagtttgtaa cttaggaatc atacctgcat ttgctatacc ttgttctatt agactttctg cattacttac tgatatttca gataacaagt taccattaac atcagtaact ccaggtacgt tcgttaaaat aatcaatttt gcagcagcaa gggcatttgc taatgcacca gctactaaat ctgcatttac gttataagta agatcatttt caccactaca tacaggagct attactggaa taaaatcaga ttcttccata aaaaataata agtctgtatt cacctcatga ggttctccaa caaaacccat gtctagtatt ttttcaacat tatttgactg attttcctta taagtataac aaactttttt tgcttctatt aaattagcat ctttaccaca taatccaata gcattacctc cagctttgtt tattagttgt gtaatatctt tattgactaa tccacataat accatttcta cgatttccaa agtttcagca tcagtaactc ttaatccatt aacaaatgta cttttcttat ttatcttctc tagaaattcg ttgattttgc tgccgccacc gtgcacaact attggattaa tacctaattg ttttaacaat acaatatcgt gagcaaaatt ttctgctaat tttctatcct tcatagcagc accaccatat ttaatgataa atgtctcacc agaaaactgc tgtatatacg gtaaggattc tgataaaact cttgctgcgt taaaccaatc agcatttcca ccaaattgct caatgtttga tacaacatct tctttattat tattttttaa tacattgtgc aatttcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLHNVLKN NNKEDVVSNI EQFGGNADWF NAARVLSESL PYIQQFSGET FIIKYGGAAM KDRKLAENFA HDIVLLKQLG INPIVVHGGG SKINEFLEKI NKKSTFVNGL RVTDAETLEI VEMVLCGLVN KDITQLINKA GGNAIGLCGK DANLIEAKKV CYTYKENQSN NVEKILDMGF VGEPHEVNTD LLFFMEESDF IPVIAPVCSG ENDLTYNVNA DLVAGALANA LAAAKLIILT NVPGVTDVNG NLLSEISVSN AESLIEQGIA NAGMIPKLQT CIKVVKEGYG SAHIIDGRIP HVLLLELFTI HGTGTMVLNT DI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaattgcac aatgtattaa aaaataataa taaagaagat gttgtatcaa acattgagca atttggtgga aatgctgatt ggtttaacgc agcaagagtt ttatcagaat ccttaccgta tatacagcag ttttctggtg agacatttat cattaaatat ggtggtgctg ctatgaagga tagaaaatta gcagaaaatt ttgctcacga tattgtattg ttaaaacaat taggtattaa tccaatagtt gtgcacggtg gcggcagcaa aatcaacgaa tttctagaga agataaataa gaaaagtaca tttgttaatg gattaagagt tactgatgct gaaactttgg aaatcgtaga aatggtatta tgtggattag tcaataaaga tattacacaa ctaataaaca aagctggagg taatgctatt ggattatgtg gtaaagatgc taatttaata gaagcaaaaa aagtttgtta tacttataag gaaaatcagt caaataatgt tgaaaaaata ctagacatgg gttttgttgg agaacctcat gaggtgaata cagacttatt attttttatg gaagaatctg attttattcc agtaatagct cctgtatgta gtggtgaaaa tgatcttact tataacgtaa atgcagattt agtagctggt gcattagcaa atgcccttgc tgctgcaaaa ttgattattt taacgaacgt acctggagtt actgatgtta atggtaactt gttatctgaa atatcagtaa gtaatgcaga aagtctaata gaacaaggta tagcaaatgc aggtatgatt cctaagttac aaacttgcat taaagtagta aaagaaggtt atggatcagc tcacataata gacggtagaa taccacacgt attgttgtta gaattgttta ccatacatgg aactggtact atggtattaa atacagatat aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for EhchA.01333.a.A1.PW27421
PURIFICATION DATe: 10/26/2009
CONCENTRATION: 23.69mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMKLHNVL KNNNKEDVVS NIEQFGGNAD WFNAARVLSE SLPYIQQFSG ETFIIKYGGA AMKDRKLAEN FAHDIVLLKQ LGINPIVVHG GGSKINEFLE KINKKSTFVN GLRVTDAETL EIVEMVLCGL VNKDITQLIN KAGGNAIGLC GKDANLIEAK KVCYTYKENQ SNNVEKILDM GFVGEPHEVN TDLLFFMEES DFIPVIAPVC SGENDLTYNV NADLVAGALA NALAAAKLII LTNVPGVTDV NGNLLSEISV SNAESLIEQG IANAGMIPKL QTCIKVVKEG YGSAHIIDGR IPHVLLLELF TIHGTGTMVL NT
Validated NT Sequence
ctgtatttaa taccatagta ccagttccat gtatggtaaa caattctaac aacaatacgt gtggtattct accgtctatt atgtgagctg atccataacc ttcttttact actttaatgc aagtttgtaa cttaggaatc atacctgcat ttgctatacc ttgttctatt agactttctg cattacttac tgatatttca gataacaagt taccattaac atcagtaact ccaggtacgt tcgttaaaat aatcaatttt gcagcagcaa gggcatttgc taatgcacca gctactaaat ctgcatttac gttataagta agatcatttt caccactaca tacaggagct attactggaa taaaatcaga ttcttccata aaaaataata agtctgtatt cacctcatga ggttctccaa caaaacccat gtctagtatt ttttcaacat tatttgactg attttcctta taagtataac aaactttttt tgcttctatt aaattagcat ctttaccaca taatccaata gcattacctc cagctttgtt tattagttgt gtaatatctt tattgactaa tccacataat accatttcta cgatttccaa agtttcagca tcagtaactc ttaatccatt aacaaatgta cttttcttat ttatcttctc tagaaattcg ttgattttgc tgccgccacc gtgcacaact attggattaa tacctaattg ttttaacaat acaatatcgt gagcaaaatt ttctgctaat tttctatcct tcatagcagc accaccatat ttaatgataa atgtctcacc agaaaactgc tgtatatacg gtaaggattc tgataaaact cttgctgcgt taaaccaatc agcatttcca ccaaattgct caatgtttga tacaacatct tctttattat tattttttaa tacattgtgc aatttcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLHNVLKN NNKEDVVSNI EQFGGNADWF NAARVLSESL PYIQQFSGET FIIKYGGAAM KDRKLAENFA HDIVLLKQLG INPIVVHGGG SKINEFLEKI NKKSTFVNGL RVTDAETLEI VEMVLCGLVN KDITQLINKA GGNAIGLCGK DANLIEAKKV CYTYKENQSN NVEKILDMGF VGEPHEVNTD LLFFMEESDF IPVIAPVCSG ENDLTYNVNA DLVAGALANA LAAAKLIILT NVPGVTDVNG NLLSEISVSN AESLIEQGIA NAGMIPKLQT CIKVVKEGYG SAHIIDGRIP HVLLLELFTI HGTGTMVLNT DI
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAATTGCAC AATGTATTAA AAAATAATAA TAAAGAAGAT GTTGTATCAA ACATTGAGCA ATTTGGTGGA AATGCTGATT GGTTTAACGC AGCAAGAGTT TTATCAGAAT CCTTACCGTA TATACAGCAG TTTTCTGGTG AGACATTTAT CATTAAATAT GGTGGTGCTG CTATGAAGGA TAGAAAATTA GCAGAAAATT TTGCTCACGA TATTGTATTG TTAAAACAAT TAGGTATTAA TCCAATAGTT GTGCACGGTG GCGGCAGCAA AATCAACGAA TTTCTAGAGA AGATAAATAA GAAAAGTACA TTTGTTAATG GATTAAGAGT TACTGATGCT GAAACTTTGG AAATCGTAGA AATGGTATTA TGTGGATTAG TCAATAAAGA TATTACACAA CTAATAAACA AAGCTGGAGG TAATGCTATT GGATTATGTG GTAAAGATGC TAATTTAATA GAAGCAAAAA AAGTTTGTTA TACTTATAAG GAAAATCAGT CAAATAATGT TGAAAAAATA CTAGACATGG GTTTTGTTGG AGAACCTCAT GAGGTGAATA CAGACTTATT ATTTTTTATG GAAGAATCTG ATTTTATTCC AGTAATAGCT CCTGTATGTA GTGGTGAAAA TGATCTTACT TATAACGTAA ATGCAGATTT AGTAGCTGGT GCATTAGCAA ATGCCCTTGC TGCTGCAAAA TTGATTATTT TAACGAACGT ACCTGGAGTT ACTGATGTTA ATGGTAACTT GTTATCTGAA ATATCAGTAA GTAATGCAGA AAGTCTAATA GAACAAGGTA TAGCAAATGC AGGTATGATT CCTAAGTTAC AAACTTGCAT TAAAGTAGTA AAAGAAGGTT ATGGATCAGC TCACATAATA GACGGTAGAA TACCACACGT ATTGTTGTTA GAATTGTTTA CCATACATGG AACTGGTACT ATGGTATTAA ATACAGATAT AAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA