EhchA.01332.a

aspartate carbamoyltransferase catalytic subunit

CENTER ID: EhchA.01332.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01332.a.A1.GE27414 Full length( EhchA.01332.a ) 1 299
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.539
RefSeq: YP_507431.1
UniProt: Q2GGK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKTLLEVSNL TSDDIESIFN LTIQYFNNTN LNHNILTGKV IVNLFFESST RTLSSFEIAE KSLGAHSITL NINTSSINKG ESIIDTISNI DAMNPDLIII RSQYSQFIKK ISEYLPSCSI INAGDGHHEH PTQALTDYCT IRYIKKDINN LNISICGDIL HSRVARSNIR LLSRYGANIS LVAPPTLSCN LTGISHIYHN LIEGIRNADV IMLLRLQKER IINCVIPSEQ EYSHLYMLNH EKLLHAKKDV IVMHPGPTNK GIEISNNVAE KNSVILLQVK MGVAARKAIL HYLLYNKSI
NT Sequence
ATGAAGACAC TATTAGAGGT AAGTAATCTA ACATCTGATG ACATAGAATC TATTTTTAAC CTTACAATAC AGTACTTTAA TAATACAAAC TTAAACCATA ATATCCTAAC TGGAAAAGTC ATTGTTAACC TATTTTTTGA AAGCTCAACA CGTACTTTAT CTTCTTTTGA AATTGCAGAA AAATCACTAG GTGCACATTC TATTACTTTA AACATTAACA CATCATCTAT AAATAAAGGA GAAAGCATTA TAGACACTAT TTCAAATATA GATGCAATGA ATCCTGATTT AATTATCATA AGGTCGCAAT ACAGTCAATT TATCAAAAAA ATATCAGAAT ATCTACCAAG CTGTTCTATA ATAAATGCCG GAGATGGACA TCATGAACAC CCAACACAAG CATTAACAGA TTACTGTACT ATACGTTACA TAAAAAAAGA TATTAATAAT TTAAATATAT CTATATGTGG AGATATATTA CACAGTAGAG TCGCAAGATC AAATATAAGA TTATTATCCA GATATGGAGC TAATATATCT TTAGTTGCTC CCCCAACATT ATCATGTAAC TTAACAGGTA TATCACATAT ATATCACAAC TTAATTGAAG GAATACGCAA TGCAGATGTC ATCATGTTGC TAAGGCTACA AAAAGAACGC ATTATTAATT GTGTTATTCC TTCAGAACAA GAATACTCTC ACCTATATAT GCTAAACCAT GAAAAACTAT TACATGCAAA AAAAGACGTC ATAGTTATGC ATCCAGGACC TACAAATAAA GGGATAGAAA TCTCCAATAA CGTGGCAGAA AAAAATTCTG TAATTCTATT ACAAGTAAAA ATGGGTGTTG CAGCACGTAA AGCTATATTA CATTATTTAC TGTACAATAA GTCAATA
Details for EhchA.01332.a.A1.GE27414
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 93
Validated AA Sequence
MKTLLEVSNL TSDDIESIFN LTIQYFNNTX LNHNILTGKV IVNLFFESST RTLSSFETAX KSLXAHSITL NINTSSINKG ESIIDTISNI XAMNPDLIII RSQYSQFIKK ISEYLPSCSI INAGDGHHEH PTQALTDYCT IRYIKKDINN LNISICGDIL HSRVARSNIR LLSRYGANIS LVAPPTLSCN LTGISHIYHN LIEGIRNADV IMLLRLQKER IINCVIPSEQ EYSHLYMLNH EKLLHAKKDV IVMHPGPTNK GIEISNNVAE KNSVILLQVK MGVAARKAIL HYLLYNKSI
Validated NT Sequence
gtcctggttc gatgaagaca ctattagagg taagtaatct aacatctgat gacatagaat ctatttttaa ccttacaata cagtacttta ataatacnna cttaaaccat aatatcctaa ctggaaaagt cattgttaac ctattttttg aaagctcaac acgtacttta tcttcttttg aaactgcana aaaatcacta ngtgcacatt ctattacttt aaacattaac acatcatcta taaataaagg agaaagcatt atagacacta tttcaaatat anatgcaatg aatcctgatt taattatcat aaggtcgcaa tacagtcaat ttatcaaaaa aatatcagaa tatctaccaa gctgttctat aataaatgcc ggagatggac atcatgaaca cccaacacaa gcattaacag attactgtac tatacgttac ataaaaaaag atattaataa tttaaatata tctatatgtg gagatatatt acacagtaga gtcgcaagat caaatataag attattatcc agatatggag ctaatatatc tttagttgct cccccaacat tatcatgtaa cttaacaggt atatcacata tatatcacaa cttaattgaa ggaatacgca atgcagatgt catcatgttg ctaaggctac aaaaagaacg cattattaat tgtgttattc cttcagaaca agaatactct cacctatata tgctaaacca tgaaaaacta ttacatgcaa aaaaagacgt catagttatg catccaggac ctacaaataa agggatagaa atctccaata acgtggcaga aaaaaattct gtaattctat tacaagtaaa aatgggtgtt gcagcacgta aagctatatt acattattta ctgtacaata agtcaatata ataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKTLLEVSN LTSDDIESIF NLTIQYFNNT NLNHNILTGK VIVNLFFESS TRTLSSFEIA EKSLGAHSIT LNINTSSINK GESIIDTISN IDAMNPDLII IRSQYSQFIK KISEYLPSCS IINAGDGHHE HPTQALTDYC TIRYIKKDIN NLNISICGDI LHSRVARSNI RLLSRYGANI SLVAPPTLSC NLTGISHIYH NLIEGIRNAD VIMLLRLQKE RIINCVIPSE QEYSHLYMLN HEKLLHAKKD VIVMHPGPTN KGIEISNNVA EKNSVILLQV KMGVAARKAI LHYLLYNKSI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagacacta ttagaggtaa gtaatctaac atctgatgac atagaatcta tttttaacct tacaatacag tactttaata atacaaactt aaaccataat atcctaactg gaaaagtcat tgttaaccta ttttttgaaa gctcaacacg tactttatct tcttttgaaa ttgcagaaaa atcactaggt gcacattcta ttactttaaa cattaacaca tcatctataa ataaaggaga aagcattata gacactattt caaatataga tgcaatgaat cctgatttaa ttatcataag gtcgcaatac agtcaattta tcaaaaaaat atcagaatat ctaccaagct gttctataat aaatgccgga gatggacatc atgaacaccc aacacaagca ttaacagatt actgtactat acgttacata aaaaaagata ttaataattt aaatatatct atatgtggag atatattaca cagtagagtc gcaagatcaa atataagatt attatccaga tatggagcta atatatcttt agttgctccc ccaacattat catgtaactt aacaggtata tcacatatat atcacaactt aattgaagga atacgcaatg cagatgtcat catgttgcta aggctacaaa aagaacgcat tattaattgt gttattcctt cagaacaaga atactctcac ctatatatgc taaaccatga aaaactatta catgcaaaaa aagacgtcat agttatgcat ccaggaccta caaataaagg gatagaaatc tccaataacg tggcagaaaa aaattctgta attctattac aagtaaaaat gggtgttgca gcacgtaaag ctatattaca ttatttactg tacaataagt caataaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa