EhchA.01316.a

succinyl-CoA synthetase, alpha subunit

CENTER ID: EhchA.01316.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01316.a.A1.GE27420 Full length( EhchA.01316.a ) 1 293
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.845
RefSeq: YP_507767.1
UniProt: Q2GFL6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVLVNSNTK VICQGFTGAH GTFHSEQAIA YGTNMVGGVT PGKGGTYHLN LPVFNTVKQA KLEAKANATV IYVPAPYAAD AILEAIEAEI ELIVCITEGI PTLDMVQVKH ALLNSSSTLI GPNCPGIITP EECKIGIMPG YIHKRGHIGI VSRSGTLTYE AVAQTTEVGL GQSTCIGIGG DPIHGMSFTD CIRLFINDPD THGIIMIGEI GGSEEEEAAE FIKLSGTNKP VVGFIAGKTA PPGKRMGHAG AISSGTSGSA ESKIEAMKNA GIIIAETPAV IGKTMLEAMN NKR
NT Sequence
ATGTCCGTTT TAGTAAATAG CAACACAAAA GTTATATGCC AGGGATTTAC TGGTGCTCAT GGTACATTTC ATTCAGAACA AGCTATTGCT TATGGAACAA ACATGGTAGG TGGAGTAACT CCTGGAAAAG GTGGAACATA TCATCTAAAC CTACCAGTGT TTAATACTGT AAAGCAAGCC AAACTAGAGG CAAAAGCTAA TGCTACTGTT ATTTATGTTC CAGCTCCTTA CGCAGCAGAT GCAATACTAG AAGCAATTGA AGCTGAGATA GAACTTATTG TTTGCATTAC AGAAGGTATA CCTACCCTAG ATATGGTACA GGTAAAACAT GCTTTACTCA ACTCATCAAG TACATTAATA GGACCAAACT GTCCTGGAAT TATCACCCCA GAAGAATGCA AAATTGGTAT TATGCCTGGA TATATACATA AACGTGGTCA CATAGGTATT GTTTCACGTT CTGGAACACT CACATATGAA GCAGTAGCAC AAACCACAGA AGTGGGGTTA GGACAATCAA CATGTATTGG TATAGGTGGA GATCCTATCC ATGGAATGTC TTTTACAGAT TGCATTAGAC TATTTATAAA TGATCCAGAT ACACATGGTA TTATCATGAT TGGAGAGATT GGAGGATCAG AAGAAGAAGA AGCTGCAGAA TTTATCAAAT TATCAGGAAC TAACAAACCT GTAGTTGGCT TCATCGCTGG CAAGACTGCA CCCCCAGGAA AACGTATGGG ACACGCTGGA GCGATTTCTT CAGGTACCTC AGGTAGTGCT GAAAGCAAAA TAGAAGCTAT GAAAAATGCA GGTATTATTA TTGCTGAAAC TCCAGCAGTC ATAGGCAAAA CAATGTTAGA AGCTATGAAT AATAAACGC
Details for EhchA.01316.a.A1.GE27420
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 32kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 92
Validated AA Sequence
MSVLVNXNTX VICQGFTGAH GTFHSXQAIA YGTNMVGGVT PXKXGTYHLN LPVFNTVKQA KLXAKANATV IYVPAPYAAD AILEAIEAEI ELIVCITEGI PTLXMVQVKH ALLNSSSTLI GPXXPGIITP EECKIGXXPG YIHKRGHIGI VSRSGTLTYE AVAQTTEVGL GQSTCIGIGG DPIHGMSFTD CIRLFINDPD THGIIMIGEI GGSEEEEAAE FIKLSGTNKP VVGFIAGKTA PPGKRMGHAG AISSGTSGSA ESKIEAMKNA GIIIAETPAV IGKTMLEAMN N
Validated NT Sequence
tattattcat agcttctaac attgttttgc ctatgactgc tggagtttca gcaataataa tacctgcatt tttcatagct tctattttgc tttcagcact acctgaggta cctgaagaaa tcgctccagc gtgtcccata cgttttcctg ggggtgcagt cttgccagcg atgaagccaa ctacaggttt gttagttcct gataatttga taaattctgc agcttcttct tcttctgatc ctccaatctc tccaatcatg ataataccat gtgtatctgg atcatttata aatagtctaa tgcaatctgt aaaagacatt ccatggatag gatctccacc tataccaata catgttgatt gtcctaaccc cacttctgtg gtttgtgcta ctgcttcata tgtgagtgtt ccagaacgtg aaacaatacc tatgtgacca cgtttatgta tatatccagg cnnnnnacca attttgcatt cttctggggt gataattcca ggacnntttg gtcctattaa tgtacttgat gagttgagta aagcatgctt tacctgtacc atatntaggg taggtatacc ttctgtaatg caaacaataa gttctatctc agcttcaatt gcttctagta tngcatctgc tgcgtaagga gctggaacat aaataacagt agcattagct tttgcntnta gtttggcttg ctttacagta ttaaacactg gtaggtttag atgatatgtt ccncnttttn caggagttac tccacctacc atgtttgttc cataagcaat agcttgttnt gaatgaaatg taccatgagc accagtaaat ccctggcata taacttntgt gttgntattt actaaaacgg acatcgaacc aggaccctgg gtctga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVLVNSNT KVICQGFTGA HGTFHSEQAI AYGTNMVGGV TPGKGGTYHL NLPVFNTVKQ AKLEAKANAT VIYVPAPYAA DAILEAIEAE IELIVCITEG IPTLDMVQVK HALLNSSSTL IGPNCPGIIT PEECKIGIMP GYIHKRGHIG IVSRSGTLTY EAVAQTTEVG LGQSTCIGIG GDPIHGMSFT DCIRLFINDP DTHGIIMIGE IGGSEEEEAA EFIKLSGTNK PVVGFIAGKT APPGKRMGHA GAISSGTSGS AESKIEAMKN AGIIIAETPA VIGKTMLEAM NNKR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtccgtttta gtaaatagca acacaaaagt tatatgccag ggatttactg gtgctcatgg tacatttcat tcagaacaag ctattgctta tggaacaaac atggtaggtg gagtaactcc tggaaaaggt ggaacatatc atctaaacct accagtgttt aatactgtaa agcaagccaa actagaggca aaagctaatg ctactgttat ttatgttcca gctccttacg cagcagatgc aatactagaa gcaattgaag ctgagataga acttattgtt tgcattacag aaggtatacc taccctagat atggtacagg taaaacatgc tttactcaac tcatcaagta cattaatagg accaaactgt cctggaatta tcaccccaga agaatgcaaa attggtatta tgcctggata tatacataaa cgtggtcaca taggtattgt ttcacgttct ggaacactca catatgaagc agtagcacaa accacagaag tggggttagg acaatcaaca tgtattggta taggtggaga tcctatccat ggaatgtctt ttacagattg cattagacta tttataaatg atccagatac acatggtatt atcatgattg gagagattgg aggatcagaa gaagaagaag ctgcagaatt tatcaaatta tcaggaacta acaaacctgt agttggcttc atcgctggca agactgcacc cccaggaaaa cgtatgggac acgctggagc gatttcttca ggtacctcag gtagtgctga aagcaaaata gaagctatga aaaatgcagg tattattatt gctgaaactc cagcagtcat aggcaaaaca atgttagaag ctatgaataa taaacgcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa