EhchA.01310.a

serine hydroxymethyltransferase

CENTER ID: EhchA.01310.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01310.a.A1.GE27422 Full length( EhchA.01310.a ) 1 420
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01310.a.A1.PW27415 Full length( EhchA.01310.a ) 1 420

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.265
RefSeq: YP_507132.1
UniProt: Q2GHF1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVGYILDDDL QEVDAEVFNC ISGELNRQNS GLQLIASENF VSKAVLQAQG SIFTNKYAEG YPGKRYYCGC HFADIVENLA IERLCRLFGC KFANVQPHSG SQANQGVFAA LLKPGDTVIG MSLDCGGHLT HGSAPSISGK WFNAVQYQVD RDTGLIDMDE IEKLAVEHNP SLIIAGSSSY PRVIDFKRFR EIADKVGAYL LADIAHYAGL IAAGEFPSPV EYAHVITSTT HKTLRGPRGA VIMTNYEDIH KKIQSSIFPG MQGGPLMHVI AAKAVAFAEA LKPEFKDYAK QIIKNSKALG EVFKERGLDL VTGGTDSHMV VLDLRSKSVT GKDAVLALEK LGIICNKNAI PFDPEKPFVT SGLRFGSAAE TSRGLQESEF REIGSMVCDV IDSLKASDSV RLSVERDIIK RVKELTSNFI
NT Sequence
ATGGTTGGGT ATATCTTAGA TGATGATTTA CAGGAAGTTG ATGCTGAGGT TTTTAATTGT ATTTCTGGAG AGTTAAATCG ACAAAATTCT GGACTACAGT TAATAGCGTC AGAAAATTTT GTGAGTAAGG CAGTATTGCA AGCTCAGGGT TCTATATTTA CAAATAAGTA TGCTGAAGGT TATCCAGGAA AAAGATATTA TTGTGGATGT CATTTTGCAG ATATTGTAGA AAATTTGGCA ATAGAACGTT TATGTCGATT GTTTGGATGT AAATTTGCAA ATGTTCAACC GCATTCTGGT TCACAAGCTA ATCAAGGAGT ATTTGCTGCA TTGTTAAAGC CAGGTGACAC TGTCATTGGT ATGTCACTTG ATTGTGGTGG TCATTTAACT CATGGTTCTG CTCCTAGTAT TTCTGGAAAG TGGTTTAATG CTGTGCAGTA TCAAGTTGAT CGTGATACTG GTTTAATAGA TATGGATGAA ATAGAGAAGT TAGCTGTTGA ACATAATCCA TCATTAATTA TTGCTGGTTC ATCTTCTTAT CCTCGTGTAA TAGATTTTAA AAGGTTTAGG GAAATAGCTG ATAAAGTGGG AGCATATCTT CTAGCTGATA TAGCGCATTA TGCTGGGTTA ATTGCTGCTG GAGAATTTCC TTCGCCTGTT GAATATGCTC ATGTTATTAC GTCTACTACT CACAAGACAT TGCGTGGCCC TAGGGGAGCA GTGATAATGA CTAATTATGA AGATATCCAT AAGAAAATTC AATCTTCTAT TTTTCCTGGT ATGCAGGGTG GTCCGTTAAT GCATGTTATT GCTGCTAAAG CTGTAGCATT TGCAGAAGCA TTGAAACCTG AGTTTAAAGA TTATGCGAAA CAAATAATAA AAAATTCTAA GGCTTTGGGT GAAGTATTTA AAGAAAGAGG GCTTGATCTT GTGACAGGTG GTACAGATTC TCATATGGTT GTTCTTGATT TACGTTCAAA AAGTGTTACA GGTAAAGATG CAGTTCTTGC TTTAGAAAAA CTAGGAATTA TTTGTAATAA AAATGCTATT CCTTTTGATC CAGAAAAGCC TTTTGTAACT TCTGGTTTAC GTTTTGGTAG TGCTGCTGAG ACTTCCAGAG GGTTACAAGA ATCAGAATTT AGGGAAATTG GCAGTATGGT TTGTGATGTT ATTGATAGTT TAAAAGCTAG TGATAGTGTT AGATTATCTG TTGAAAGAGA TATAATAAAA AGAGTTAAGG AATTAACTTC TAATTTTATT
Details for EhchA.01310.a.A1.GE27422
HARVESTED ON: 9/15/2009
SEQUENCED ON: 10/20/2009
EXPECTED MW: 48kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGYILDDD LQEVDAEVFN CISGELNRQN SGLQLIASEN FVSKAVLQAQ GSIFTNKYAE GYPGKRYYCG CHFADIVENL AIERLCRLFG CKFANVQPHS GSQANQGVFA ALLKPGDTVI GMSLDCGGHL THGSAPSISG KWFNAVQYQV DRDTGLIDMD EIEKLAVEHN PSLIIAGSSS YPRVIDFKRF REIADKVGAY LLADIAHYAG LIAAGEFPSP VEYAHVITST THKTLRGPRG AVIMTNYEDI HKKIQSSIFP GMQGGPLMHV IAAKAVAFAE ALKPEFKDYA KQIIKNSKAL GEVFKERGLD LVTGGTDSHM VVLDLRSKSV TGKDAVLALE KLGIICNKNA IPFDPEKPFV TSGLRFGSAA ETSRGLQESE FREIGSMVCD VIDSLKASDS VRLSVERDII KRVKELTSNF I
Validated NT Sequence
ttattaaata aaattagaag ttaattcctt aactcttttt attatatctc tttcaacaga taatctaaca ctatcactag cttttaaact atcaataaca tcacaaacca tactgccaat ttccctaaat tctgattctt gtaaccctct ggaagtctca gcagcactac caaaacgtaa accagaagtt acaaaaggct tttctggatc aaaaggaata gcatttttat tacaaataat tcctagtttt tctaaagcaa gaactgcatc tttacctgta acactttttg aacgtaaatc aagaacaacc atatgagaat ctgtaccacc tgtcacaaga tcaagccctc tttctttaaa tacttcaccc aaagccttag aattttttat tatttgtttc gcataatctt taaactcagg tttcaatgct tctgcaaatg ctacagcttt agcagcaata acatgcatta acggaccacc ctgcatacca ggaaaaatag aagattgaat tttcttatgg atatcttcat aattagtcat tatcactgct cccctagggc cacgcaatgt cttgtgagta gtagacgtaa taacatgagc atattcaaca ggcgaaggaa attctccagc agcaattaac ccagcataat gcgctatatc agctagaaga tatgctccca ctttatcagc tatttcccta aaccttttaa aatctattac acgaggataa gaagatgaac cagcaataat taatgatgga ttatgttcaa cagctaactt ctctatttca tccatatcta ttaaaccagt atcacgatca acttgatact gcacagcatt aaaccacttt ccagaaatac taggagcaga accatgagtt aaatgaccac cacaatcaag tgacatacca atgacagtgt cacctggctt taacaatgca gcaaatactc cttgattagc ttgtgaacca gaatgcggtt gaacatttgc aaatttacat ccaaacaatc gacataaacg ttctattgcc aaattttcta caatatctgc aaaatgacat ccacaataat atctttttcc tggataacct tcagcatact tatttgtaaa tatagaaccc tgagcttgca atactgcctt actcacaaaa ttttctgacg ctattaactg tagtccagaa ttttgtcgat ttaactctcc agaaatacaa ttaaaaacct cagcatcaac ttcctgtaaa tcatcatcta agatataccc aaccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGYILDDD LQEVDAEVFN CISGELNRQN SGLQLIASEN FVSKAVLQAQ GSIFTNKYAE GYPGKRYYCG CHFADIVENL AIERLCRLFG CKFANVQPHS GSQANQGVFA ALLKPGDTVI GMSLDCGGHL THGSAPSISG KWFNAVQYQV DRDTGLIDMD EIEKLAVEHN PSLIIAGSSS YPRVIDFKRF REIADKVGAY LLADIAHYAG LIAAGEFPSP VEYAHVITST THKTLRGPRG AVIMTNYEDI HKKIQSSIFP GMQGGPLMHV IAAKAVAFAE ALKPEFKDYA KQIIKNSKAL GEVFKERGLD LVTGGTDSHM VVLDLRSKSV TGKDAVLALE KLGIICNKNA IPFDPEKPFV TSGLRFGSAA ETSRGLQESE FREIGSMVCD VIDSLKASDS VRLSVERDII KRVKELTSNF I
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttgggtat atcttagatg atgatttaca ggaagttgat gctgaggttt ttaattgtat ttctggagag ttaaatcgac aaaattctgg actacagtta atagcgtcag aaaattttgt gagtaaggca gtattgcaag ctcagggttc tatatttaca aataagtatg ctgaaggtta tccaggaaaa agatattatt gtggatgtca ttttgcagat attgtagaaa atttggcaat agaacgttta tgtcgattgt ttggatgtaa atttgcaaat gttcaaccgc attctggttc acaagctaat caaggagtat ttgctgcatt gttaaagcca ggtgacactg tcattggtat gtcacttgat tgtggtggtc atttaactca tggttctgct cctagtattt ctggaaagtg gtttaatgct gtgcagtatc aagttgatcg tgatactggt ttaatagata tggatgaaat agagaagtta gctgttgaac ataatccatc attaattatt gctggttcat cttcttatcc tcgtgtaata gattttaaaa ggtttaggga aatagctgat aaagtgggag catatcttct agctgatata gcgcattatg ctgggttaat tgctgctgga gaatttcctt cgcctgttga atatgctcat gttattacgt ctactactca caagacattg cgtggcccta ggggagcagt gataatgact aattatgaag atatccataa gaaaattcaa tcttctattt ttcctggtat gcagggtggt ccgttaatgc atgttattgc tgctaaagct gtagcatttg cagaagcatt gaaacctgag tttaaagatt atgcgaaaca aataataaaa aattctaagg ctttgggtga agtatttaaa gaaagagggc ttgatcttgt gacaggtggt acagattctc atatggttgt tcttgattta cgttcaaaaa gtgttacagg taaagatgca gttcttgctt tagaaaaact aggaattatt tgtaataaaa atgctattcc ttttgatcca gaaaagcctt ttgtaacttc tggtttacgt tttggtagtg ctgctgagac ttccagaggg ttacaagaat cagaatttag ggaaattggc agtatggttt gtgatgttat tgatagttta aaagctagtg atagtgttag attatctgtt gaaagagata taataaaaag agttaaggaa ttaacttcta attttattaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EhchA.01310.a.A1.PW27415
PURIFICATION DATe: 11/2/2009
CONCENTRATION: 27.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVGYILDDD LQEVDAEVFN CISGELNRQN SGLQLIASEN FVSKAVLQAQ GSIFTNKYAE GYPGKRYYCG CHFADIVENL AIERLCRLFG CKFANVQPHS GSQANQGVFA ALLKPGDTVI GMSLDCGGHL THGSAPSISG KWFNAVQYQV DRDTGLIDMD EIEKLAVEHN PSLIIAGSSS YPRVIDFKRF REIADKVGAY LLADIAHYAG LIAAGEFPSP VEYAHVITST THKTLRGPRG AVIMTNYEDI HKKIQSSIFP GMQGGPLMHV IAAKAVAFAE ALKPEFKDYA KQIIKNSKAL GEVFKERGLD LVTGGTDSHM VVLDLRSKSV TGKDAVLALE KLGIICNKNA IPFDPEKPFV TSGLRFGSAA ETSRGLQESE FREIGSMVCD VIDSLKASDS VRLSVERDII KRVKELTSNF I
Validated NT Sequence
ttattaaata aaattagaag ttaattcctt aactcttttt attatatctc tttcaacaga taatctaaca ctatcactag cttttaaact atcaataaca tcacaaacca tactgccaat ttccctaaat tctgattctt gtaaccctct ggaagtctca gcagcactac caaaacgtaa accagaagtt acaaaaggct tttctggatc aaaaggaata gcatttttat tacaaataat tcctagtttt tctaaagcaa gaactgcatc tttacctgta acactttttg aacgtaaatc aagaacaacc atatgagaat ctgtaccacc tgtcacaaga tcaagccctc tttctttaaa tacttcaccc aaagccttag aattttttat tatttgtttc gcataatctt taaactcagg tttcaatgct tctgcaaatg ctacagcttt agcagcaata acatgcatta acggaccacc ctgcatacca ggaaaaatag aagattgaat tttcttatgg atatcttcat aattagtcat tatcactgct cccctagggc cacgcaatgt cttgtgagta gtagacgtaa taacatgagc atattcaaca ggcgaaggaa attctccagc agcaattaac ccagcataat gcgctatatc agctagaaga tatgctccca ctttatcagc tatttcccta aaccttttaa aatctattac acgaggataa gaagatgaac cagcaataat taatgatgga ttatgttcaa cagctaactt ctctatttca tccatatcta ttaaaccagt atcacgatca acttgatact gcacagcatt aaaccacttt ccagaaatac taggagcaga accatgagtt aaatgaccac cacaatcaag tgacatacca atgacagtgt cacctggctt taacaatgca gcaaatactc cttgattagc ttgtgaacca gaatgcggtt gaacatttgc aaatttacat ccaaacaatc gacataaacg ttctattgcc aaattttcta caatatctgc aaaatgacat ccacaataat atctttttcc tggataacct tcagcatact tatttgtaaa tatagaaccc tgagcttgca atactgcctt actcacaaaa ttttctgacg ctattaactg tagtccagaa ttttgtcgat ttaactctcc agaaatacaa ttaaaaacct cagcatcaac ttcctgtaaa tcatcatcta agatataccc aaccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg gtat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVGYILDDD LQEVDAEVFN CISGELNRQN SGLQLIASEN FVSKAVLQAQ GSIFTNKYAE GYPGKRYYCG CHFADIVENL AIERLCRLFG CKFANVQPHS GSQANQGVFA ALLKPGDTVI GMSLDCGGHL THGSAPSISG KWFNAVQYQV DRDTGLIDMD EIEKLAVEHN PSLIIAGSSS YPRVIDFKRF REIADKVGAY LLADIAHYAG LIAAGEFPSP VEYAHVITST THKTLRGPRG AVIMTNYEDI HKKIQSSIFP GMQGGPLMHV IAAKAVAFAE ALKPEFKDYA KQIIKNSKAL GEVFKERGLD LVTGGTDSHM VVLDLRSKSV TGKDAVLALE KLGIICNKNA IPFDPEKPFV TSGLRFGSAA ETSRGLQESE FREIGSMVCD VIDSLKASDS VRLSVERDII KRVKELTSNF I
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTTGGGTAT ATCTTAGATG ATGATTTACA GGAAGTTGAT GCTGAGGTTT TTAATTGTAT TTCTGGAGAG TTAAATCGAC AAAATTCTGG ACTACAGTTA ATAGCGTCAG AAAATTTTGT GAGTAAGGCA GTATTGCAAG CTCAGGGTTC TATATTTACA AATAAGTATG CTGAAGGTTA TCCAGGAAAA AGATATTATT GTGGATGTCA TTTTGCAGAT ATTGTAGAAA ATTTGGCAAT AGAACGTTTA TGTCGATTGT TTGGATGTAA ATTTGCAAAT GTTCAACCGC ATTCTGGTTC ACAAGCTAAT CAAGGAGTAT TTGCTGCATT GTTAAAGCCA GGTGACACTG TCATTGGTAT GTCACTTGAT TGTGGTGGTC ATTTAACTCA TGGTTCTGCT CCTAGTATTT CTGGAAAGTG GTTTAATGCT GTGCAGTATC AAGTTGATCG TGATACTGGT TTAATAGATA TGGATGAAAT AGAGAAGTTA GCTGTTGAAC ATAATCCATC ATTAATTATT GCTGGTTCAT CTTCTTATCC TCGTGTAATA GATTTTAAAA GGTTTAGGGA AATAGCTGAT AAAGTGGGAG CATATCTTCT AGCTGATATA GCGCATTATG CTGGGTTAAT TGCTGCTGGA GAATTTCCTT CGCCTGTTGA ATATGCTCAT GTTATTACGT CTACTACTCA CAAGACATTG CGTGGCCCTA GGGGAGCAGT GATAATGACT AATTATGAAG ATATCCATAA GAAAATTCAA TCTTCTATTT TTCCTGGTAT GCAGGGTGGT CCGTTAATGC ATGTTATTGC TGCTAAAGCT GTAGCATTTG CAGAAGCATT GAAACCTGAG TTTAAAGATT ATGCGAAACA AATAATAAAA AATTCTAAGG CTTTGGGTGA AGTATTTAAA GAAAGAGGGC TTGATCTTGT GACAGGTGGT ACAGATTCTC ATATGGTTGT TCTTGATTTA CGTTCAAAAA GTGTTACAGG TAAAGATGCA GTTCTTGCTT TAGAAAAACT AGGAATTATT TGTAATAAAA ATGCTATTCC TTTTGATCCA GAAAAGCCTT TTGTAACTTC TGGTTTACGT TTTGGTAGTG CTGCTGAGAC TTCCAGAGGG TTACAAGAAT CAGAATTTAG GGAAATTGGC AGTATGGTTT GTGATGTTAT TGATAGTTTA AAAGCTAGTG ATAGTGTTAG ATTATCTGTT GAAAGAGATA TAATAAAAAG AGTTAAGGAA TTAACTTCTA ATTTTATTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA