SSGCID: Target Page

EhchA.01289.a

putative heme exporter protein CcmA

CENTER ID:	EhchA.01289.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01289.a.A1.GE27421	Full length( EhchA.01289.a )		1	217

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.249
RefSeq:	YP_507116.1
UniProt:	Q2GHG7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLECTDLACI RNNRYLFSQL NFSTQQNLLT LVIGNNGSGK TSLLRLFAGL VPITHGNIKY NGKNIHDSNT YFTSLTYIGH KNFCNDNFTV LENIQLWANL KNTHELIAAT ICFFKLQPIL DIKYKDLSSG WKRRVALSRL LIYNTNIWII DEPFAHLDIN TKELILELIF TRLKQKDSIV IISDHSEVKT YGECQVINLE AFKNQQSNSL QHDLYLY

NT Sequence

ATGCTAGAAT GTACTGACCT TGCTTGCATT AGAAATAATA GATATCTATT CTCCCAGCTT AATTTTAGTA CACAACAAAA CTTACTAACA TTAGTTATAG GGAATAATGG AAGCGGCAAA ACAAGCTTAC TTAGACTATT TGCAGGACTA GTCCCCATAA CACATGGAAA CATAAAATAT AATGGAAAAA ACATCCACGA TTCCAATACA TATTTTACAT CTCTTACTTA TATAGGGCAC AAAAACTTTT GCAATGACAA TTTCACTGTC CTAGAAAATA TCCAACTATG GGCTAACTTA AAAAATACCC ATGAGTTAAT AGCAGCTACA ATATGCTTTT TTAAATTACA ACCAATACTA GACATAAAAT ACAAGGATTT ATCATCTGGG TGGAAAAGAA GAGTCGCACT ATCCAGATTA TTAATATATA ACACCAACAT ATGGATTATA GATGAGCCCT TTGCTCACCT TGACATTAAT ACAAAAGAAT TAATATTAGA ACTCATTTTT ACAAGATTAA AACAAAAAGA TAGTATAGTA ATTATATCTG ACCACAGCGA AGTAAAAACA TATGGAGAAT GTCAAGTTAT CAACCTTGAA GCTTTTAAAA ATCAGCAATC TAATAGTTTA CAGCATGATC TCTATCTATA C

Details for EhchA.01289.a.A1.GE27421

HARVESTED ON:	9/15/2009
SEQUENCED ON:	9/30/2009
EXPECTED MW:	27kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLECTDLAC IRNNRYLFSQ LNFSTQQNLL TLVIGNNGSG KTSLLRLFAG LVPITHGNIK YNGKNIHDSN TYFTSLTYIG HKNFCNDNFT VLENIQLWAN LKNTHELIAA TICFFKLQPI LDIKYKDLSS GWKRRVALSR LLIYNTNIWI IDEPFAHLDI NTKELILELI FTRLKQKDSI VIISDHSEVK TYGECQVINL EAFKNQQSNS LQHDLYLY

Validated NT Sequence

ttattagtat agatagagat catgctgtaa actattagat tgctgatttt taaaagcttc aaggttgata acttgacatt ctccatatgt ttttacttcg ctgtggtcag atataattac tatactatct ttttgtttta atcttgtaaa aatgagttct aatattaatt cttttgtatt aatgtcaagg tgagcaaagg gctcatctat aatccatatg ttggtgttat atattaataa tctggatagt gcgactcttc ttttccaccc agatgataaa tccttgtatt ttatgtctag tattggttgt aatttaaaaa agcatattgt agctgctatt aactcatggg tattttttaa gttagcccat agttggatat tttctaggac agtgaaattg tcattgcaaa agtttttgtg ccctatataa gtaagagatg taaaatatgt attggaatcg tggatgtttt ttccattata ttttatgttt ccatgtgtta tggggactag tcctgcaaat agtctaagta agcttgtttt gccgcttcca ttattcccta taactaatgt tagtaagttt tgttgtgtac taaaattaag ctgngagaat agatatctat tatttctaat gcaagcaagg tcagtacatt ctagcatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat ggtat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctagaatgt actgaccttg cttgcattag aaataataga tatctattct cccagcttaa ttttagtaca caacaaaact tactaacatt agttataggg aataatggaa gcggcaaaac aagcttactt agactatttg caggactagt ccccataaca catggaaaca taaaatataa tggaaaaaac atccacgatt ccaatacata ttttacatct cttacttata tagggcacaa aaacttttgc aatgacaatt tcactgtcct agaaaatatc caactatggg ctaacttaaa aaatacccat gagttaatag cagctacaat atgctttttt aaattacaac caatactaga cataaaatac aaggatttat catctgggtg gaaaagaaga gtcgcactat ccagattatt aatatataac accaacatat ggattataga tgagcccttt gctcaccttg acattaatac aaaagaatta atattagaac tcatttttac aagattaaaa caaaaagata gtatagtaat tatatctgac cacagcgaag taaaaacata tggagaatgt caagttatca accttgaagc ttttaaaaat cagcaatcta atagtttaca gcatgatctc tatctataca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a