EhchA.01256.a

type IV secretion system protein VirB11

CENTER ID:	EhchA.01256.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01256.a.A1.GE27408	Full length( EhchA.01256.a )		1	332

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_130713
PATRIC ID:	fig\|205920.11.peg.39
RefSeq:	YP_506873.1
UniProt:	Q2GI60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTSYAALDT YLEPLQSIFH EDGVNEVSIN QECEVWIENR GNIRCESIPT LNLSHLKALG RLIAQATEQK ISEENPLLSA TLPNGYRIQI VFPPACEATT VVMSIRKPSA MQLSLDDYEK MGAFNQAVTE TQENNTDNQL KELLKQKKIK QFLEHAIINK KNIIISGGTS TGKTTFTNAA LRSIPQNERL ITVEDAREIV LSNHPNRVHL IASKGGQGRA KVSTQDLIEA CLRLRPDRII VGELRGSEAF SFLRAINTGH PGSISTLHAD TPMMALEQLK LMVMQAGLGI PPDQIINYIT NIVDIVIQLK RGSEGKRYVS EILFTKSLQK NG

NT Sequence

ATGACAACAA GCTATGCCGC CTTAGATACG TATCTCGAAC CACTACAATC CATCTTTCAT GAAGATGGGG TAAATGAAGT ATCTATTAAC CAGGAATGTG AAGTATGGAT AGAAAACAGA GGAAATATAC GTTGTGAATC CATTCCGACC CTAAATTTAT CGCATTTAAA AGCATTAGGC AGACTAATAG CACAAGCTAC AGAACAAAAG ATAAGCGAAG AAAATCCATT ATTATCAGCA ACTTTACCTA ATGGCTATCG TATACAAATC GTATTTCCTC CAGCCTGTGA AGCAACCACA GTAGTCATGT CAATTAGAAA ACCATCAGCA ATGCAACTTT CATTAGATGA CTATGAAAAA ATGGGCGCAT TTAACCAAGC TGTAACAGAA ACTCAAGAAA ATAATACAGA TAATCAATTA AAAGAACTAT TAAAGCAAAA AAAGATCAAG CAATTTCTAG AACATGCTAT AATAAACAAA AAAAACATAA TAATTAGTGG TGGTACATCA ACAGGAAAAA CAACTTTTAC CAATGCAGCA TTACGAAGTA TACCTCAGAA TGAAAGGCTA ATAACAGTAG AAGATGCAAG AGAAATTGTT TTAAGTAATC ATCCAAACAG AGTCCATTTA ATTGCCTCAA AAGGAGGACA AGGTAGAGCT AAAGTCAGCA CTCAAGATTT AATAGAGGCA TGTCTAAGAT TGCGTCCAGA TAGGATAATA GTAGGAGAAT TACGTGGATC TGAAGCATTT AGCTTTTTAA GAGCAATTAA TACAGGCCAT CCTGGTTCCA TATCAACGTT ACACGCTGAC ACACCAATGA TGGCACTAGA ACAATTAAAA TTAATGGTTA TGCAAGCAGG ACTTGGCATA CCTCCAGATC AAATAATTAA TTACATCACT AATATAGTAG ATATTGTAAT ACAGTTAAAA AGAGGATCAG AGGGTAAAAG GTATGTATCA GAAATATTAT TCACTAAATC TTTACAGAAA AATGGT

Details for EhchA.01256.a.A1.GE27408

HARVESTED ON:	9/15/2009
SEQUENCED ON:	9/30/2009
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	88
PERCENT COVERAGE:	75

Validated AA Sequence

XXXXXXXXXG YPGSSXPGSX FDDXSYAALX TYLEPLXSIF HEDGVNEVSI NXECEVWIEN XXNIRCESIP TLNLSHLXAL GRLIAQATEQ KISEENXLLS ATLPNGYRXX IVFPPACEAT TVXMSIRKPS AMQLXXDDYE KMGAFNQAXX XTXENXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXSNHPNRXH LIXSXGGQGR VKXXTQDLIE ARXXXXPDRI IVGELRGSEA FSFLRAINTG HPGSISTLHA DTPMMAXEQL KLMVMQAGLG IPPDQIINYI TNIVDIVIQL KRGSEGKRYV SEILFTKSLQ KNG

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTTSYAALD TYLEPLQSIF HEDGVNEVSI NQECEVWIEN RGNIRCESIP TLNLSHLKAL GRLIAQATEQ KISEENPLLS ATLPNGYRIQ IVFPPACEAT TVVMSIRKPS AMQLSLDDYE KMGAFNQAVT ETQENNTDNQ LKELLKQKKI KQFLEHAIIN KKNIIISGGT STGKTTFTNA ALRSIPQNER LITVEDAREI VLSNHPNRVH LIASKGGQGR AKVSTQDLIE ACLRLRPDRI IVGELRGSEA FSFLRAINTG HPGSISTLHA DTPMMALEQL KLMVMQAGLG IPPDQIINYI TNIVDIVIQL KRGSEGKRYV SEILFTKSLQ KNG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaacaagc tatgccgcct tagatacgta tctcgaacca ctacaatcca tctttcatga agatggggta aatgaagtat ctattaacca ggaatgtgaa gtatggatag aaaacagagg aaatatacgt tgtgaatcca ttccgaccct aaatttatcg catttaaaag cattaggcag actaatagca caagctacag aacaaaagat aagcgaagaa aatccattat tatcagcaac tttacctaat ggctatcgta tacaaatcgt atttcctcca gcctgtgaag caaccacagt agtcatgtca attagaaaac catcagcaat gcaactttca ttagatgact atgaaaaaat gggcgcattt aaccaagctg taacagaaac tcaagaaaat aatacagata atcaattaaa agaactatta aagcaaaaaa agatcaagca atttctagaa catgctataa taaacaaaaa aaacataata attagtggtg gtacatcaac aggaaaaaca acttttacca atgcagcatt acgaagtata cctcagaatg aaaggctaat aacagtagaa gatgcaagag aaattgtttt aagtaatcat ccaaacagag tccatttaat tgcctcaaaa ggaggacaag gtagagctaa agtcagcact caagatttaa tagaggcatg tctaagattg cgtccagata ggataatagt aggagaatta cgtggatctg aagcatttag ctttttaaga gcaattaata caggccatcc tggttccata tcaacgttac acgctgacac accaatgatg gcactagaac aattaaaatt aatggttatg caagcaggac ttggcatacc tccagatcaa ataattaatt acatcactaa tatagtagat attgtaatac agttaaaaag aggatcagag ggtaaaaggt atgtatcaga aatattattc actaaatctt tacagaaaaa tggtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa