SSGCID: Target Page

EhchA.01248.a

diaminopimelate decarboxylase

CENTER ID:	EhchA.01248.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01248.a.A1.GE27416	Full length( EhchA.01248.a )		1	420

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01248.a.A1.PS00404	Full length( EhchA.01248.a )		1	420

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.418
RefSeq:	YP_507301.1
UniProt:	Q2GGY2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIFLANPFFH YKNNILSIEN VNVLDIAKNV GTPVYCYSLN AIKNNYRQFQ ENLPNNSIVC YAVKSNSNLS ILSLLNSLGA GADVVSEGEI RRAIAAGISP NKIVFSGVGK TEQEINFALD NRILQFNVES IEELSLINTI ACQKNIIAPV SIRINPNINA DTNDKITTGL KVNKFGIPEE LLDQIFNKDF TWIKIIGISV HIGSQISNLH IFQNTIDKIK KIIKIFKQHN TQIIRVDLGG GLGIPYKSSD TFPTIQEYAN LLKKNFENEN YQIICEPGRA LVGNTGILLT KVLYRKYNQE KSHIILDAGM NDLIRPALYN AEHTIIPAIS STSTQLQPCD IVGPICESDD TFAHNYHIHN LQNGEIVAIC TAGAYGSSMS SNYNSRLLIP EVMVNNSSYD IIRSRQTYED MLKDEIILEF

NT Sequence

ATGATCTTTC TTGCTAATCC ATTTTTTCAT TATAAAAACA ACATACTCAG TATAGAAAAC GTAAATGTAT TGGATATTGC AAAAAATGTC GGAACCCCTG TCTATTGCTA TTCTTTAAAT GCAATAAAAA ATAACTATAG ACAATTCCAA GAAAATCTAC CTAATAATTC TATAGTATGT TATGCAGTTA AGTCGAATTC TAACTTATCT ATTTTAAGTT TATTAAATTC ACTTGGAGCA GGAGCAGACG TTGTATCTGA AGGAGAAATA CGACGTGCTA TTGCAGCTGG AATTTCACCA AATAAAATAG TTTTCTCTGG CGTAGGAAAA ACTGAGCAAG AAATAAATTT CGCATTGGAC AATAGAATAC TACAATTTAA TGTAGAGTCT ATTGAAGAGT TATCTCTCAT AAATACAATA GCATGTCAAA AAAATATAAT TGCACCAGTA TCTATTAGGA TTAATCCGAA TATTAATGCA GATACAAATG ATAAAATCAC AACAGGATTG AAAGTTAATA AATTTGGTAT ACCAGAAGAA TTACTTGATC AAATTTTCAA TAAAGACTTT ACTTGGATTA AAATTATAGG AATATCCGTA CACATAGGTT CACAAATTTC CAATCTACAC ATATTCCAAA ATACAATTGA CAAAATAAAA AAAATTATAA AAATTTTTAA GCAACATAAT ACTCAAATTA TCAGGGTTGA CCTTGGTGGA GGACTTGGAA TACCATATAA AAGTTCTGAT ACCTTCCCAA CAATTCAAGA ATATGCAAAC TTACTAAAAA AGAATTTTGA AAATGAAAAT TATCAAATCA TATGTGAACC TGGTAGAGCA TTAGTAGGAA ATACTGGTAT ACTATTAACA AAAGTACTAT ACCGTAAATA TAATCAAGAA AAAAGTCACA TTATATTAGA TGCAGGAATG AATGACCTAA TACGTCCAGC ATTATACAAC GCTGAACACA CAATAATACC AGCAATTTCT TCAACATCAA CACAACTACA ACCATGCGAT ATAGTCGGCC CTATATGTGA ATCTGATGAT ACTTTTGCAC ATAATTATCA TATACATAAT TTACAAAACG GAGAAATAGT AGCAATATGC ACAGCTGGAG CTTATGGATC TTCAATGTCA AGTAACTATA ACTCCAGATT ATTAATACCT GAAGTAATGG TTAACAACAG TAGTTATGAC ATTATTCGTT CTAGGCAAAC TTATGAAGAC ATGTTAAAAG ATGAGATCAT ATTAGAATTT

Details for EhchA.01248.a.A1.GE27416

HARVESTED ON:	9/15/2009
SEQUENCED ON:	10/20/2009
EXPECTED MW:	49kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	9

Validated AA Sequence

MSSNYNSRLL IPEVMVNNSS YDIIRSRQTY EDMLKDEIIL

Validated NT Sequence

ctaatatgat ctcatctttt aacatgtctt cataagtttg cctagaacga ataatgtcat aactactgtt gttaaccatt acttcaggta ttaataatct ggagttatag ttacttgaca ttgaagatcc ataagctcca gctgtgcata ttgctactat ttctccgttt tgtaaattat gtatatgata attatgtgca aaagtatcat cagattcaca tatngggccg actatatcgc atggttgtag ttgtgttgat gttgaanaaa ttgctggtat tattgtgtgt tcngcgttgt ataangctgg acgtatta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMIFLANPFF HYKNNILSIE NVNVLDIAKN VGTPVYCYSL NAIKNNYRQF QENLPNNSIV CYAVKSNSNL SILSLLNSLG AGADVVSEGE IRRAIAAGIS PNKIVFSGVG KTEQEINFAL DNRILQFNVE SIEELSLINT IACQKNIIAP VSIRINPNIN ADTNDKITTG LKVNKFGIPE ELLDQIFNKD FTWIKIIGIS VHIGSQISNL HIFQNTIDKI KKIIKIFKQH NTQIIRVDLG GGLGIPYKSS DTFPTIQEYA NLLKKNFENE NYQIICEPGR ALVGNTGILL TKVLYRKYNQ EKSHIILDAG MNDLIRPALY NAEHTIIPAI SSTSTQLQPC DIVGPICESD DTFAHNYHIH NLQNGEIVAI CTAGAYGSSM SSNYNSRLLI PEVMVNNSSY DIIRSRQTYE DMLKDEIILE F

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatctttctt gctaatccat tttttcatta taaaaacaac atactcagta tagaaaacgt aaatgtattg gatattgcaa aaaatgtcgg aacccctgtc tattgctatt ctttaaatgc aataaaaaat aactatagac aattccaaga aaatctacct aataattcta tagtatgtta tgcagttaag tcgaattcta acttatctat tttaagttta ttaaattcac ttggagcagg agcagacgtt gtatctgaag gagaaatacg acgtgctatt gcagctggaa tttcaccaaa taaaatagtt ttctctggcg taggaaaaac tgagcaagaa ataaatttcg cattggacaa tagaatacta caatttaatg tagagtctat tgaagagtta tctctcataa atacaatagc atgtcaaaaa aatataattg caccagtatc tattaggatt aatccgaata ttaatgcaga tacaaatgat aaaatcacaa caggattgaa agttaataaa tttggtatac cagaagaatt acttgatcaa attttcaata aagactttac ttggattaaa attataggaa tatccgtaca cataggttca caaatttcca atctacacat attccaaaat acaattgaca aaataaaaaa aattataaaa atttttaagc aacataatac tcaaattatc agggttgacc ttggtggagg acttggaata ccatataaaa gttctgatac cttcccaaca attcaagaat atgcaaactt actaaaaaag aattttgaaa atgaaaatta tcaaatcata tgtgaacctg gtagagcatt agtaggaaat actggtatac tattaacaaa agtactatac cgtaaatata atcaagaaaa aagtcacatt atattagatg caggaatgaa tgacctaata cgtccagcat tatacaacgc tgaacacaca ataataccag caatttcttc aacatcaaca caactacaac catgcgatat agtcggccct atatgtgaat ctgatgatac ttttgcacat aattatcata tacataattt acaaaacgga gaaatagtag caatatgcac agctggagct tatggatctt caatgtcaag taactataac tccagattat taatacctga agtaatggtt aacaacagta gttatgacat tattcgttct aggcaaactt atgaagacat gttaaaagat gagatcatat tagaatttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa

Details for EhchA.01248.a.A1.PS00404

PURIFICATION DATe:	11/3/2009
CONCENTRATION:	38.2mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	9

Protocol Notes

notes unavailable

Validated AA Sequence

MSSNYNSRLL IPEVMVNNSS YDIIRSRQTY EDMLKDEIIL

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GATCTTTCTT GCTAATCCAT TTTTTCATTA TAAAAACAAC ATACTCAGTA TAGAAAACGT AAATGTATTG GATATTGCAA AAAATGTCGG AACCCCTGTC TATTGCTATT CTTTAAATGC AATAAAAAAT AACTATAGAC AATTCCAAGA AAATCTACCT AATAATTCTA TAGTATGTTA TGCAGTTAAG TCGAATTCTA ACTTATCTAT TTTAAGTTTA TTAAATTCAC TTGGAGCAGG AGCAGACGTT GTATCTGAAG GAGAAATACG ACGTGCTATT GCAGCTGGAA TTTCACCAAA TAAAATAGTT TTCTCTGGCG TAGGAAAAAC TGAGCAAGAA ATAAATTTCG CATTGGACAA TAGAATACTA CAATTTAATG TAGAGTCTAT TGAAGAGTTA TCTCTCATAA ATACAATAGC ATGTCAAAAA AATATAATTG CACCAGTATC TATTAGGATT AATCCGAATA TTAATGCAGA TACAAATGAT AAAATCACAA CAGGATTGAA AGTTAATAAA TTTGGTATAC CAGAAGAATT ACTTGATCAA ATTTTCAATA AAGACTTTAC TTGGATTAAA ATTATAGGAA TATCCGTACA CATAGGTTCA CAAATTTCCA ATCTACACAT ATTCCAAAAT ACAATTGACA AAATAAAAAA AATTATAAAA ATTTTTAAGC AACATAATAC TCAAATTATC AGGGTTGACC TTGGTGGAGG ACTTGGAATA CCATATAAAA GTTCTGATAC CTTCCCAACA ATTCAAGAAT ATGCAAACTT ACTAAAAAAG AATTTTGAAA ATGAAAATTA TCAAATCATA TGTGAACCTG GTAGAGCATT AGTAGGAAAT ACTGGTATAC TATTAACAAA AGTACTATAC CGTAAATATA ATCAAGAAAA AAGTCACATT ATATTAGATG CAGGAATGAA TGACCTAATA CGTCCAGCAT TATACAACGC TGAACACACA ATAATACCAG CAATTTCTTC AACATCAACA CAACTACAAC CATGCGATAT AGTCGGCCCT ATATGTGAAT CTGATGATAC TTTTGCACAT AATTATCATA TACATAATTT ACAAAACGGA GAAATAGTAG CAATATGCAC AGCTGGAGCT TATGGATCTT CAATGTCAAG TAACTATAAC TCCAGATTAT TAATACCTGA AGTAATGGTT AACAACAGTA GTTATGACAT TATTCGTTCT AGGCAAACTT ATGAAGACAT GTTAAAAGAT GAGATCATAT TAGAATTTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA