SSGCID: Target Page

EhchA.01240.a

thiamine-phosphate pyrophosphorylase

CENTER ID:	EhchA.01240.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01240.a.A1.GE27412	Full length( EhchA.01240.a )		1	244

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.775
RefSeq:	YP_507688.1
UniProt:	Q2GFU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQKDALIIAR ASANQSVEDA SFLDERYFPI ITLNKDYYFD KNFHPMLDPV GFYQIVPDLF WLKYVINLGV KVVQLRIKNE PIEEVEYKIK EGVHIANQNG VKLFVNDYWQ FAVKYKAYGV HLGQEDLRSA NFNEIYNAGL RLGISTHCYH ELAIAKYIRP SYIAFGPIFP TTLKNMNFMP QGTDLLNYWV KNLPYKIVAI GGINLSNVDS VIGTGVDGIA VISAVLNSEY PDKVVHEFIQ KCQI

NT Sequence

TTGCAAAAGG ATGCTTTAAT TATAGCAAGA GCTTCTGCTA ATCAATCTGT TGAAGATGCT AGTTTTTTAG ATGAAAGGTA TTTCCCTATT ATTACGCTTA ATAAAGATTA TTATTTTGAT AAAAATTTTC ATCCTATGCT TGATCCTGTA GGTTTTTACC AAATAGTGCC AGATCTATTT TGGTTGAAGT ATGTAATTAA TTTAGGGGTT AAAGTAGTAC AGCTGAGAAT TAAAAATGAA CCAATAGAAG AAGTAGAGTA TAAAATAAAG GAAGGTGTAC ATATTGCAAA TCAAAATGGT GTAAAATTGT TTGTGAATGA TTATTGGCAG TTTGCAGTAA AGTATAAAGC TTATGGAGTG CATTTAGGAC AGGAGGACTT GAGGAGTGCA AACTTTAATG AAATATATAA TGCAGGATTG AGACTAGGTA TTAGTACCCA TTGTTATCAT GAATTAGCAA TAGCGAAATA TATACGTCCT TCATATATTG CTTTTGGCCC TATTTTTCCT ACTACATTAA AAAACATGAA TTTTATGCCA CAGGGTACAG ATTTACTTAA TTATTGGGTT AAGAATTTAC CTTATAAAAT TGTGGCTATT GGTGGCATTA ATTTGTCAAA TGTGGATTCA GTAATTGGAA CTGGTGTTGA CGGCATAGCT GTTATATCTG CTGTGTTAAA TAGTGAATAT CCTGATAAAG TGGTACATGA GTTTATACAA AAATGCCAAA TC

Details for EhchA.01240.a.A1.GE27412

HARVESTED ON:	9/15/2009
SEQUENCED ON:	10/20/2009
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLQKDALII ARASANQSVE DASFLDERYF PIITLNKDYY FDKNFHPMLD PVGFYQIVPD LFWLKYVINL GVKVVQLRIK NEPIEEVEYK IKEGVHIANQ NGVKLFVNDY WQFAVKYKAY GVHLGQEDLR SANFNEIYNA GLRLGISTHC YHELAIAKYI RPSYIAFGPI FPTTLKNMNF MPQGTDLLNY WVKNLPYKIV AIGGINLSNV DSVIGTGVDG IAVISAVLNS EYPDKVVHEF IQK

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc aaaaggatgc tttaattata gcaagagctt ctgctaatca atctgttgaa gatgctagtt ttttagatga aaggtatttc cctattatta cgcttaataa agattattat tttgataaaa attttcatcc tatgcttgat cctgtaggtt tttaccaaat agtgccagat ctattttggt tgaagtatgt aattaattta ggggttaaag tagtacagct gagaattaaa aatgaaccaa tagaagaagt agagtataaa ataaaggaag gtgtacatat tgcaaatcaa aatggtgtaa aattgtttgt gaatgattat tggcagtttg cagtaaagta taaagcttat ggagtgcatt taggacagga ggacttgagg agtgcaaact ttaatgaaat atataatgca ggattgagac taggtattag tacccattgt tatcatgaat tagcaatagc gaaatatata cgtccttcat atattgcttt tggccctatt tttcctacta cattaaaaaa catgaatttt atgccacagg gtacagattt acttaattat tgggttaaga atttacctta taaaattgtg gctattggtg gcattaattt gtcaaatgtg gattcagtaa ttggaactgg tgttgacggc atagctgtta tatctgctgt gttaaatagt gaatatcctg ataaagtggt acatgagttt atacaaaaat g

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcaaaag gatgctttaa ttatagcaag agcttctgct aatcaatctg ttgaagatgc tagtttttta gatgaaaggt atttccctat tattacgctt aataaagatt attattttga taaaaatttt catcctatgc ttgatcctgt aggtttttac caaatagtgc cagatctatt ttggttgaag tatgtaatta atttaggggt taaagtagta cagctgagaa ttaaaaatga accaatagaa gaagtagagt ataaaataaa ggaaggtgta catattgcaa atcaaaatgg tgtaaaattg tttgtgaatg attattggca gtttgcagta aagtataaag cttatggagt gcatttagga caggaggact tgaggagtgc aaactttaat gaaatatata atgcaggatt gagactaggt attagtaccc attgttatca tgaattagca atagcgaaat atatacgtcc ttcatatatt gcttttggcc ctatttttcc tactacatta aaaaacatga attttatgcc acagggtaca gatttactta attattgggt taagaattta ccttataaaa ttgtggctat tggtggcatt aatttgtcaa atgtggattc agtaattgga actggtgttg acggcatagc tgttatatct gctgtgttaa atagtgaata tcctgataaa gtggtacatg agtttataca aaaatgccaa atcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa