SSGCID: Target Page

EhchA.01201.a

ABC transporter, ATP-binding protein

CENTER ID:	EhchA.01201.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01201.a.A1.GE30612	Full length( EhchA.01201.a )		1	235

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.149
RefSeq:	YP_506999.1
UniProt:	Q2GHT4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTVFALANI YKSFGRNKEV PVIVNATLQI KKGEIVALIG KSGSGKSTLL HIAGLLDTPS SGSILLNNIE CTDKTSDKDK TYLRRHFLGF IYQFHHLLQE FSVLENVMLP QIITGKSKSI AKKNAMEILE RVRLQDKLSM PISQLSGGER QRVAIARSLI NCPLIVLADE PTGSLDNNTA LEVFSLLQEY AKEKNIAILL ATHNYSLAQK ACRIVKIDSG ILRSYSIDES GFNKI

NT Sequence

ATGTCTACAG TATTTGCTCT AGCTAATATT TACAAATCTT TTGGTAGGAA TAAAGAAGTA CCAGTTATAG TAAATGCAAC TTTACAAATA AAAAAAGGAG AAATTGTTGC TTTAATAGGT AAATCAGGAT CAGGCAAAAG TACATTATTA CACATTGCTG GATTACTAGA CACTCCATCT TCAGGGAGTA TATTGCTCAA TAATATAGAG TGCACAGATA AAACCTCAGA CAAAGATAAA ACATATTTAC GTCGCCATTT TCTAGGATTC ATTTACCAAT TTCATCATTT ACTACAGGAG TTTTCAGTAT TAGAAAACGT GATGTTACCA CAAATCATTA CAGGTAAAAG TAAGTCCATT GCAAAAAAAA ATGCTATGGA AATTTTAGAA CGCGTAAGAC TACAAGATAA ATTGTCAATG CCAATATCAC AGCTTTCTGG AGGAGAAAGA CAACGCGTTG CAATAGCAAG AAGCTTAATC AATTGTCCTC TGATAGTTCT AGCAGACGAA CCAACTGGTA GTCTAGATAA CAATACTGCA CTTGAAGTAT TTTCTTTGCT ACAAGAATAT GCAAAAGAAA AAAATATTGC TATACTCCTT GCAACACATA ACTACAGCCT AGCTCAAAAA GCATGTCGTA TCGTTAAAAT AGATTCAGGT ATACTGAGAA GTTATAGCAT TGATGAATCA GGCTTCAACA AAATA

Details for EhchA.01201.a.A1.GE30612

HARVESTED ON:	10/27/2010
SEQUENCED ON:	11/3/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSTVFALAN IYKSFGRNKE VPVIVNATLQ IKKGEIVALI GKSGSGKSTL LHIAGLLDTP SSGSILLNNI ECTDKTSDKD KTYLRRHFLG FIYQFHHLLQ EFSVLENVML PQIITGKSKS IAKKNAMEIL ERVRLQDKLS MPISQLSGGE RQRVAIARSL INCPLIVLAD EPTGSLDNNT ALEVFSLLQE YAKEKNIAIL LATHNYSLAQ KACRIVKIDS GILRSYSIDE SGFN

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcta cagtatttgc tctagctaat atttacaaat cttttggtag gaataaagaa gtaccagtta tagtaaatgc aactttacaa ataaaaaaag gagaaattgt tgctttaata ggtaaatcag gatcaggcaa aagtacatta ttacacattg ctggattact agacactcca tcttcaggga gtatattgct caataatata gagtgcacag ataaaacctc agacaaagat aaaacatatt tacgtcgcca ttttctagga ttcatttacc aatttcatca tttactacag gagttttcag tattagaaaa cgtgatgtta ccacaaatca ttacaggtaa aagtaagtcc attgcaaaaa aaaatgctat ggaaatttta gaacgcgtaa gactacaaga taaattgtca atgccaatat cacagctttc tggaggagaa agacaacgcg ttgcaatagc aagaagctta atcaattgtc ctctgatagt tctagcagac gaaccaactg gtagtctaga taacaatact gcacttgaag tattttcttt gctacaagaa tatgcaaaag aaaaaaatat tgctatactc cttgcaacac ataactacag cctagctcaa aaagcatgtc gtatcgttaa aatagattca ggtatactga gaagttatag cattgatgaa tcaggcttca aca

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctacagta tttgctctag ctaatattta caaatctttt ggtaggaata aagaagtacc agttatagta aatgcaactt tacaaataaa aaaaggagaa attgttgctt taataggtaa atcaggatca ggcaaaagta cattattaca cattgctgga ttactagaca ctccatcttc agggagtata ttgctcaata atatagagtg cacagataaa acctcagaca aagataaaac atatttacgt cgccattttc taggattcat ttaccaattt catcatttac tacaggagtt ttcagtatta gaaaacgtga tgttaccaca aatcattaca ggtaaaagta agtccattgc aaaaaaaaat gctatggaaa ttttagaacg cgtaagacta caagataaat tgtcaatgcc aatatcacag ctttctggag gagaaagaca acgcgttgca atagcaagaa gcttaatcaa ttgtcctctg atagttctag cagacgaacc aactggtagt ctagataaca atactgcact tgaagtattt tctttgctac aagaatatgc aaaagaaaaa aatattgcta tactccttgc aacacataac tacagcctag ctcaaaaagc atgtcgtatc gttaaaatag attcaggtat actgagaagt tatagcattg atgaatcagg cttcaacaaa ataaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa