EhchA.01190.a
HAD-superfamily hydrolase, subfamily IA, variant 1
| CENTER ID: | EhchA.01190.a |
| ORGANISM: | Ehrlichia chaffeensis str. Arkansas |
| ASSOCIATED DISEASE: | Human monocytic ehrlichiosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EhchA.01190.a.A1.GE27403 | Full length( EhchA.01190.a ) | 1 | 210 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EhchA.01190.a.A1.PW27396 | Full length( EhchA.01190.a ) | 1 | 210 |
Structures
3KZX
DEPOSITED: 12/8/2009
DETERMINATION: XRay
CLONE: EhchA.01190.a.A1.GE27403
PROTEIN: EhchA.01190.a.A1.PW27396
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129718 |
| PATRIC ID: | fig|205920.11.peg.283 |
| RefSeq: | YP_507152.1 |
| UniProt: | Q2GHD1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKQPTAVIFD WYNTLIDTSI NIDRTTFYQV LDQMGYKNID LDSIPNSTIP KYLITLLGKR WKEATILYEN SLEKSQKSDN FMLNDGAIEL LDTLKENNIT MAIVSNKNGE RLRSEIHHKN LTHYFDSIIG SGDTGTIKPS PEPVLAALTN INIEPSKEVF FIGDSISDIQ SAIEAGCLPI KYGSTNIIKD ILSFKNFYDI RNFICQLINI
NT Sequence
ATGAAACAAC CAACAGCAGT AATATTCGAT TGGTACAATA CACTAATTGA TACCAGTATT AATATCGATC GTACAACTTT TTACCAAGTA TTGGATCAGA TGGGATATAA AAATATAGAT CTGGACTCTA TTCCCAATTC TACAATACCA AAATATCTAA TCACATTACT AGGGAAAAGG TGGAAAGAAG CAACCATCTT GTATGAGAAC AGTCTAGAGA AATCACAAAA ATCAGACAAT TTCATGCTGA ATGATGGAGC TATTGAATTA TTAGATACAC TAAAAGAAAA TAATATCACC ATGGCAATTG TAAGCAATAA GAATGGTGAA CGTTTACGAT CTGAAATACA TCATAAAAAT CTAACACACT ATTTTGACTC TATCATCGGC TCTGGAGATA CAGGAACTAT AAAACCATCC CCTGAACCAG TATTAGCAGC ATTGACAAAT ATAAATATTG AACCAAGCAA AGAAGTATTT TTCATAGGAG ATAGTATATC AGATATACAA AGTGCAATTG AAGCGGGATG CCTACCCATA AAATACGGTA GCACCAATAT TATAAAGGAT ATTCTTTCTT TTAAAAATTT TTATGATATT CGTAACTTTA TCTGTCAATT AATAAATATA
Details for EhchA.01190.a.A1.GE27403
| HARVESTED ON: | 9/15/2009 |
| SEQUENCED ON: | 9/30/2009 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 24kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKQPTAVIF DWYNTLIDTS INIDRTTFYQ VLDQMGYKNI DLDSIPNSTI PKYLITLLGK RWKEATILYE NSLEKSQKSD NFMLNDGAIE LLDTLKENNI TMAIVSNKNG ERLRSEIHHK NLTHYFDSII GSGDTGTIKP SPEPVLAALT NINIEPSKEV FFIGDSISDI QSAIEAGCLP IKYGSTNIIK DILSFKNFYD IRNFICQLIN I
Validated NT Sequence
ttattatata tttattaatt gacagataaa gttacgaata tcataaaaat ttttaaaaga aagaatatcc tttataatat tggtgctacc gtattttatg ggtaggcatc ccgcttcaat tgcactttgt atatctgata tactatctcc tatgaaaaat acttctttgc ttggttcaat atttatattt gtcaatgctg ctaatactgg ttcaggggat ggttttatag ttcctgtatc tccagagccg atgatagagt caaaatagtg tgttagattt ttatgatgta tttcagatcg taaacgttca ccattcttat tgcttacaat tgccatggtg atattatttt cttttagtgt atctaataat tcaatagctc catcattcag catgaaattg tctgattttt gtgatttctc tagactgttc tcatacaaga tggttgcttc tttccacctt ttccctagta atgtgattag atattttggt attgtagaat tgggaataga gtccagatct atatttttat atcccatctg atccaatact tggtaaaaag ttgtacgatc gatattaata ctggtatcaa ttagtgtatt gtaccaatcg aatattactg ctgttggttg tttcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKQPTAVIF DWYNTLIDTS INIDRTTFYQ VLDQMGYKNI DLDSIPNSTI PKYLITLLGK RWKEATILYE NSLEKSQKSD NFMLNDGAIE LLDTLKENNI TMAIVSNKNG ERLRSEIHHK NLTHYFDSII GSGDTGTIKP SPEPVLAALT NINIEPSKEV FFIGDSISDI QSAIEAGCLP IKYGSTNIIK DILSFKNFYD IRNFICQLIN I
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaacaacca acagcagtaa tattcgattg gtacaataca ctaattgata ccagtattaa tatcgatcgt acaacttttt accaagtatt ggatcagatg ggatataaaa atatagatct ggactctatt cccaattcta caataccaaa atatctaatc acattactag ggaaaaggtg gaaagaagca accatcttgt atgagaacag tctagagaaa tcacaaaaat cagacaattt catgctgaat gatggagcta ttgaattatt agatacacta aaagaaaata atatcaccat ggcaattgta agcaataaga atggtgaacg tttacgatct gaaatacatc ataaaaatct aacacactat tttgactcta tcatcggctc tggagataca ggaactataa aaccatcccc tgaaccagta ttagcagcat tgacaaatat aaatattgaa ccaagcaaag aagtattttt cataggagat agtatatcag atatacaaag tgcaattgaa gcgggatgcc tacccataaa atacggtagc accaatatta taaaggatat tctttctttt aaaaattttt atgatattcg taactttatc tgtcaattaa taaatataaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for EhchA.01190.a.A1.PW27396
| PURIFICATION DATe: | 11/2/2009 |
| CONCENTRATION: | 26.81mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKQPTAVIF DWYNTLIDTS INIDRTTFYQ VLDQMGYKNI DLDSIPNSTI PKYLITLLGK RWKEATILYE NSLEKSQKSD NFMLNDGAIE LLDTLKENNI TMAIVSNKNG ERLRSEIHHK NLTHYFDSII GSGDTGTIKP SPEPVLAALT NINIEPSKEV FFIGDSISDI QSAIEAGCLP IKYGSTNIIK DILSFKNFYD IRNFICQLIN I
Validated NT Sequence
ttattatata tttattaatt gacagataaa gttacgaata tcataaaaat ttttaaaaga aagaatatcc tttataatat tggtgctacc gtattttatg ggtaggcatc ccgcttcaat tgcactttgt atatctgata tactatctcc tatgaaaaat acttctttgc ttggttcaat atttatattt gtcaatgctg ctaatactgg ttcaggggat ggttttatag ttcctgtatc tccagagccg atgatagagt caaaatagtg tgttagattt ttatgatgta tttcagatcg taaacgttca ccattcttat tgcttacaat tgccatggtg atattatttt cttttagtgt atctaataat tcaatagctc catcattcag catgaaattg tctgattttt gtgatttctc tagactgttc tcatacaaga tggttgcttc tttccacctt ttccctagta atgtgattag atattttggt attgtagaat tgggaataga gtccagatct atatttttat atcccatctg atccaatact tggtaaaaag ttgtacgatc gatattaata ctggtatcaa ttagtgtatt gtaccaatcg aatattactg ctgttggttg tttcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKQPTAVIF DWYNTLIDTS INIDRTTFYQ VLDQMGYKNI DLDSIPNSTI PKYLITLLGK RWKEATILYE NSLEKSQKSD NFMLNDGAIE LLDTLKENNI TMAIVSNKNG ERLRSEIHHK NLTHYFDSII GSGDTGTIKP SPEPVLAALT NINIEPSKEV FFIGDSISDI QSAIEAGCLP IKYGSTNIIK DILSFKNFYD IRNFICQLIN I
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAACAACCA ACAGCAGTAA TATTCGATTG GTACAATACA CTAATTGATA CCAGTATTAA TATCGATCGT ACAACTTTTT ACCAAGTATT GGATCAGATG GGATATAAAA ATATAGATCT GGACTCTATT CCCAATTCTA CAATACCAAA ATATCTAATC ACATTACTAG GGAAAAGGTG GAAAGAAGCA ACCATCTTGT ATGAGAACAG TCTAGAGAAA TCACAAAAAT CAGACAATTT CATGCTGAAT GATGGAGCTA TTGAATTATT AGATACACTA AAAGAAAATA ATATCACCAT GGCAATTGTA AGCAATAAGA ATGGTGAACG TTTACGATCT GAAATACATC ATAAAAATCT AACACACTAT TTTGACTCTA TCATCGGCTC TGGAGATACA GGAACTATAA AACCATCCCC TGAACCAGTA TTAGCAGCAT TGACAAATAT AAATATTGAA CCAAGCAAAG AAGTATTTTT CATAGGAGAT AGTATATCAG ATATACAAAG TGCAATTGAA GCGGGATGCC TACCCATAAA ATACGGTAGC ACCAATATTA TAAAGGATAT TCTTTCTTTT AAAAATTTTT ATGATATTCG TAACTTTATC TGTCAATTAA TAAATATAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA