SSGCID: Target Page

EhchA.01175.a

GTP cyclohydrolase I

CENTER ID:	EhchA.01175.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01175.a.A1.GE27399	Full length( EhchA.01175.a )		1	190

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.568
RefSeq:	YP_507460.1
UniProt:	Q2GGH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRNGKPSDSE AEEAVNLLIR WIGDDPNREG LVNTAKRVLD VYKRFFSGYQ VDIYSMQDLV LLNENYNDMV IFKNTEFTSY CEHHIVPMRG KISIGYVPDK LVFGVGKIVK LINCFTKRLQ LQEKLTVEIA NALDHCLAPK GIIVAVEAVH DCIVCCEEED KHNLELQTTC AIGVFQDNME LRREFFSSIG

NT Sequence

ATGAGAAATG GCAAACCTTC TGATAGTGAA GCCGAAGAAG CTGTTAATTT ACTAATAAGA TGGATAGGTG ATGATCCAAA TAGAGAAGGT CTTGTTAATA CTGCTAAACG TGTATTGGAT GTCTATAAAA GATTTTTTTC AGGATATCAA GTAGATATAT ATTCTATGCA AGATTTAGTT TTACTTAATG AGAATTATAA TGATATGGTG ATTTTTAAAA ATACTGAATT TACATCGTAT TGTGAGCATC ATATTGTTCC AATGAGAGGT AAAATTAGTA TTGGGTATGT TCCTGATAAG TTAGTATTTG GAGTAGGTAA AATTGTAAAA TTAATTAACT GTTTTACAAA AAGATTGCAA CTTCAAGAGA AATTAACTGT AGAAATAGCA AACGCCTTAG ATCATTGTTT AGCTCCGAAA GGGATAATTG TTGCTGTTGA AGCAGTACAT GATTGTATTG TATGCTGTGA AGAGGAAGAC AAGCATAATT TGGAATTACA AACGACTTGT GCAATTGGAG TTTTTCAAGA TAACATGGAA TTAAGAAGAG AATTTTTTTC TAGTATTGGT

Details for EhchA.01175.a.A1.GE27399

HARVESTED ON:	9/15/2009
SEQUENCED ON:	9/30/2009
EXPECTED MW:	23kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRNGKPSDS EAEEAVNLLI RWIGDDPNRE GLVNTAKRVL DVYKRFFSGY QVDIYSMQDL VLLNENYNDM VIFKNTEFTS YCEHHIVPMR GKISIGYVPD KLVFGVGKIV KLINCFTKRL QLQEKLTVEI ANALDHCLAP KGIIVAVEAV HDCIVCCEEE DKHNLELQTT CAIGVFQDNM ELRREFFSSI G

Validated NT Sequence

ttattaacca atactagaaa aaaattctct tcttaattcc atgttatctt gaaaaactcc aattgcacaa gtcgtttgta attccaaatt atgcttgtct tcctcttcac agcatacaat acaatcatgt actgcttcaa cagcaacaat tatccctttc ggagctaaac aatgatctaa ggcgtttgct atttctacag ttaatttctc ttgaagttgc aatctttttg taaaacagtt aattaatttt acaattttac ctactccaaa tactaactta tcaggaacat acccaatact aattttacct ctcattggaa caatatgatg ctcacaatac gatgtaaatt cagtattttt aaaaatcacc atatcattat aattctcatt aagtaaaact aaatcttgca tagaatatat atctacttga tatcctgaaa aaaatctttt atagacatcc aatacacgtt tagcagtatt aacaagacct tctctatttg gatcatcacc tatccatctt attagtaaat taacagcttc ttcggcttca ctatcagaag gtttgccatt tctcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagaaatggc aaaccttctg atagtgaagc cgaagaagct gttaatttac taataagatg gataggtgat gatccaaata gagaaggtct tgttaatact gctaaacgtg tattggatgt ctataaaaga tttttttcag gatatcaagt agatatatat tctatgcaag atttagtttt acttaatgag aattataatg atatggtgat ttttaaaaat actgaattta catcgtattg tgagcatcat attgttccaa tgagaggtaa aattagtatt gggtatgttc ctgataagtt agtatttgga gtaggtaaaa ttgtaaaatt aattaactgt tttacaaaaa gattgcaact tcaagagaaa ttaactgtag aaatagcaaa cgccttagat cattgtttag ctccgaaagg gataattgtt gctgttgaag cagtacatga ttgtattgta tgctgtgaag aggaagacaa gcataatttg gaattacaaa cgacttgtgc aattggagtt tttcaagata acatggaatt aagaagagaa tttttttcta gtattggtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa