SSGCID: Target Page

EhchA.01142.a

50S ribosomal protein L3

CENTER ID:	EhchA.01142.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01142.a.A1.GE27431	Full length( EhchA.01142.a )		1	231

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|205920.11.peg.346
RefSeq:	YP_507227.1
UniProt:	Q2GH56

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKRIGLFLE KVGHTAIFDN EGKRIPITLL HLRDSFIISK KTKDIHGYDA VVLGVEASIN LKKPQSKILE KSKITAKCKI FESRVDNLGD VVEGAKVSIT HFVKDQYVDI TGYSLGKGFA GVMKRHNFKG LKASHGVSIA HRSQGSTGQC QDPGRVYKGK KMAGQLGNSK VTVQNLKVIL VDQERSLLGV RGNNIPGAKG SYVFVKDAVK KSVPKGSFPI CAEDLKLDNV V

NT Sequence

ATGGCTAAGA GAATAGGATT ATTTCTAGAA AAAGTAGGCC ATACGGCAAT TTTTGATAAT GAAGGTAAAA GGATTCCAAT TACTTTATTA CATTTACGAG ATTCTTTTAT TATTTCTAAA AAGACTAAAG ATATTCATGG TTACGATGCA GTGGTATTAG GGGTAGAAGC TTCTATTAAT TTAAAGAAGC CTCAATCAAA GATTTTAGAG AAAAGCAAAA TTACTGCTAA ATGTAAGATT TTTGAATCTA GGGTTGATAA TTTAGGTGAT GTAGTAGAGG GAGCTAAAGT TTCTATTACT CATTTTGTCA AGGATCAATA TGTTGATATT ACTGGGTATT CTTTAGGGAA AGGTTTTGCT GGTGTTATGA AGCGACATAA TTTTAAAGGA TTAAAAGCAT CTCATGGTGT CTCTATTGCT CATAGATCTC AAGGCTCAAC AGGTCAGTGT CAAGATCCTG GTAGAGTATA TAAAGGGAAG AAAATGGCTG GTCAATTAGG GAATAGTAAA GTCACAGTAC AGAATCTTAA GGTTATTTTA GTTGATCAGG AGAGATCTTT ATTAGGTGTA AGAGGTAATA ATATTCCTGG GGCGAAAGGT TCTTATGTTT TTGTAAAAGA TGCTGTAAAA AAATCAGTTC CAAAGGGTTC TTTTCCTATA TGTGCTGAAG ATTTAAAGTT AGATAATGTT GTG

Details for EhchA.01142.a.A1.GE27431

HARVESTED ON:	9/15/2009
SEQUENCED ON:	10/20/2009
EXPECTED MW:	27kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	95
PERCENT COVERAGE:	43

Validated AA Sequence

MKXHNFXGLK ASHGVSIAHR XQGSXGQCQD PGRVYKGKKM XGQLGNSKVT VQNLKVILVD QERSLLGVRG NNIPGAKGSY VFVKDAVKKS VPKGSFPICA EDLKLDNVV

Validated NT Sequence

ttattacaca acattatcta actttaaatc ttcagcacat ataggaaaag aaccctttgg aactgatttt tttacagcat cttttacaaa aacataagaa cctttcgccc caggaatatt attacctctt acacctaata aagatctctc ctgatcaact aaaataacct taagattctg tactgtgact ttactattcc ctaattgacc agncattttc ttccctttat atactctacc aggatcttga cactgacctg ntgagccttg anatctatga gcaatagaga caccatgaga tgcttttaat ccntnaaaat tatgtcnctt cataacacca gcaaaacctt tccc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAKRIGLFL EKVGHTAIFD NEGKRIPITL LHLRDSFIIS KKTKDIHGYD AVVLGVEASI NLKKPQSKIL EKSKITAKCK IFESRVDNLG DVVEGAKVSI THFVKDQYVD ITGYSLGKGF AGVMKRHNFK GLKASHGVSI AHRSQGSTGQ CQDPGRVYKG KKMAGQLGNS KVTVQNLKVI LVDQERSLLG VRGNNIPGAK GSYVFVKDAV KKSVPKGSFP ICAEDLKLDN VV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctaagaga ataggattat ttctagaaaa agtaggccat acggcaattt ttgataatga aggtaaaagg attccaatta ctttattaca tttacgagat tcttttatta tttctaaaaa gactaaagat attcatggtt acgatgcagt ggtattaggg gtagaagctt ctattaattt aaagaagcct caatcaaaga ttttagagaa aagcaaaatt actgctaaat gtaagatttt tgaatctagg gttgataatt taggtgatgt agtagaggga gctaaagttt ctattactca ttttgtcaag gatcaatatg ttgatattac tgggtattct ttagggaaag gttttgctgg tgttatgaag cgacataatt ttaaaggatt aaaagcatct catggtgtct ctattgctca tagatctcaa ggctcaacag gtcagtgtca agatcctggt agagtatata aagggaagaa aatggctggt caattaggga atagtaaagt cacagtacag aatcttaagg ttattttagt tgatcaggag agatctttat taggtgtaag aggtaataat attcctgggg cgaaaggttc ttatgttttt gtaaaagatg ctgtaaaaaa atcagttcca aagggttctt ttcctatatg tgctgaagat ttaaagttag ataatgttgt gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa