EhchA.01133.a

methionine aminopeptidase

CENTER ID:	EhchA.01133.a
ORGANISM:	Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE:	Human monocytic ehrlichiosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	unclassified
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
EhchA.01133.a.A1.GE27424	Full length( EhchA.01133.a )		1	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126721
PATRIC ID:	fig\|205920.11.peg.914
RefSeq:	YP_507849.1
UniProt:	Q2GFD4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGNLGRSITI HSKEDFKYMH NAGRLAAEVL DFITPYVKPG VSSNELNDLC HDFIINAGAI PAPLGYRGYP KSICTSKNFV VCHGIPDDLP LKEGDIVNID VTVILDGWHG DTSRMYWVGE PSIKAKRLCE AAYRALTVAI EQVIPGQKLN QIGLAIEKEM KKYGYSIVRD YCGHGLGRVF HASPNVVHYY DPDDPVIIRE GMFFTIEPMI NAGRCDTVLS KKDGWTVTTR DLSLSAQFEH SLGVTENGVE IFTLSPQNMH YPPYVC

NT Sequence

ATGGGAAATT TAGGAAGAAG TATCACTATT CATAGTAAAG AAGACTTTAA GTATATGCAT AATGCTGGAA GGCTTGCAGC AGAGGTGTTA GATTTTATTA CTCCATATGT AAAACCAGGA GTTTCTAGCA ATGAGCTTAA CGATTTATGT CATGATTTTA TTATTAACGC TGGTGCAATT CCTGCTCCGC TTGGATATAG GGGATACCCT AAGTCTATTT GTACCTCGAA AAATTTTGTT GTATGTCATG GTATTCCTGA TGATTTGCCT TTAAAAGAAG GTGATATTGT GAATATAGAT GTCACTGTTA TACTAGACGG TTGGCATGGA GATACTAGTA GAATGTATTG GGTTGGGGAG CCATCGATTA AAGCAAAACG TTTATGTGAA GCTGCATATA GAGCATTAAC TGTAGCGATT GAACAAGTGA TACCTGGACA AAAGCTTAAT CAGATTGGTT TGGCTATTGA AAAAGAGATG AAGAAATATG GTTATTCTAT TGTTCGTGAT TATTGTGGGC ATGGGTTAGG TAGGGTATTT CATGCTTCGC CGAATGTAGT ACATTATTAT GATCCGGATG ATCCTGTAAT TATTAGAGAA GGCATGTTTT TTACAATAGA GCCAATGATA AATGCCGGAA GATGTGATAC TGTACTTAGT AAAAAAGATG GATGGACTGT TACAACTCGA GATTTATCTT TGTCAGCGCA ATTTGAACAT TCTTTGGGTG TTACTGAGAA TGGAGTAGAA ATATTTACAT TATCTCCACA AAATATGCAT TATCCTCCTT ATGTATGC

Details for EhchA.01133.a.A1.GE27424

HARVESTED ON:	9/15/2009
SEQUENCED ON:	9/30/2009
EXPECTED MW:	31kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMGNLGRSIT IHSKEDFKYM HNAGRLAAEV LDFITPYVKP GVSSNELNDL CHDFIINAGA IPAPLGYRGY PKSICTSKNF VVCHGIPDDL PLKEGDIVNI DVTVILDGWH GDTSRMYWVG EPSIKAKRLC EAAYRALTVA IEQVIPGQKL NQIGLAIEKE MKKYGYSIVR DYCGHGLGRV FHASPNVVHY YDPDDPVIIR EGMFFTIEPM INAGRCDTVL SKKDGWTVTT RDLSLSAQFE HSLGVTENGV EIFTLSPQNM HYPPYVC

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggaa atttaggaag aagtatcact attcatagta aagaagactt taagtatatg cataatgctg gaaggcttgc agcagaggtg ttagatttta ttactccata tgtaaaacca ggagtttcta gcaatgagct taacgattta tgtcatgatt ttattattaa cgctggtgca attcctgctc cgcttggata taggggatac cctaagtcta tttgtacctc gaaaaatttt gttgtatgtc atggtattcc tgatgatttg cctttaaaag aaggtgatat tgtgaatata gatgtcactg ttatactaga cggttggcat ggagatacta gtagaatgta ttgggttggg gagccatcga ttaaagcaaa acgtttatgt gaagctgcat atagagcatt aactgtagcg attgaacaag tgatacctgg acaaaagctt aatcagattg gtttggctat tgaaaaagag atgaagaaat atggttattc tattgttcgt gattattgtg ggcatgggtt aggtagggta tttcatgctt cgccgaatgt agtacattat tatgatccgg atgatcctgt aattattaga gaaggcatgt tttttacaat agagccaatg ataaatgccg gaagatgtga tactgtactt agtaaaaaag atggatggac tgttacaact cgagatttat ctttgtcagc gcaatttgaa cattctttgg gtgttactga gaatggagta gaaatattta cattatctcc acaaaatatg cattatcctc cttatgtatg ctaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggaaattta ggaagaagta tcactattca tagtaaagaa gactttaagt atatgcataa tgctggaagg cttgcagcag aggtgttaga ttttattact ccatatgtaa aaccaggagt ttctagcaat gagcttaacg atttatgtca tgattttatt attaacgctg gtgcaattcc tgctccgctt ggatataggg gataccctaa gtctatttgt acctcgaaaa attttgttgt atgtcatggt attcctgatg atttgccttt aaaagaaggt gatattgtga atatagatgt cactgttata ctagacggtt ggcatggaga tactagtaga atgtattggg ttggggagcc atcgattaaa gcaaaacgtt tatgtgaagc tgcatataga gcattaactg tagcgattga acaagtgata cctggacaaa agcttaatca gattggtttg gctattgaaa aagagatgaa gaaatatggt tattctattg ttcgtgatta ttgtgggcat gggttaggta gggtatttca tgcttcgccg aatgtagtac attattatga tccggatgat cctgtaatta ttagagaagg catgtttttt acaatagagc caatgataaa tgccggaaga tgtgatactg tacttagtaa aaaagatgga tggactgtta caactcgaga tttatctttg tcagcgcaat ttgaacattc tttgggtgtt actgagaatg gagtagaaat atttacatta tctccacaaa atatgcatta tcctccttat gtatgcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa