EhchA.01131.a

ribose-phosphate pyrophosphokinase

CENTER ID: EhchA.01131.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01131.a.A1.GE27398 Full length( EhchA.01131.a ) 1 318
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.883
RefSeq: YP_507810.1
UniProt: Q2GFH3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLISSGTSSI NLAKCISNIT SIKLVNSCIS RFSDQELNVE IQENQDDKNK HVIIINSLCS PAHDNLLELL LLTDAINRTL HPRKITMIIP YLCYTRQDRV MYRNLDDNSL MVSALSAKVI INILRTANIN NIIFIDLHSN QLSGFFDIAT TNLSSHTVFI GDIIEKHNTN NLVVVSPDYG ALNRTRTFAN VLSKQCKLHN EIQVAVIDKY RAKPGVSEVM NITGNVENKD CIIVDDIVDS AGTLCNAASA LKDRGALQVS AYVTHGILSG NAVEKVTNSK LDNLIITDTI HNGFFNTSKI RILSIDKFLS NYILYNLV
NT Sequence
ATGTTAATAT CTTCTGGTAC ATCTAGTATT AACTTAGCAA AGTGCATATC AAACATTACA AGTATAAAGT TAGTTAACTC TTGTATTTCT CGTTTTTCTG ATCAAGAATT AAACGTAGAA ATTCAAGAAA ACCAAGATGA CAAAAATAAA CACGTCATAA TTATAAATTC ACTTTGTTCC CCAGCACATG ATAATTTACT AGAATTACTC TTGTTAACGG ATGCAATTAA TAGAACATTG CATCCTAGAA AAATAACCAT GATAATACCT TATCTTTGTT ACACTAGACA AGACAGAGTA ATGTATAGAA ACTTAGATGA CAACAGCCTA ATGGTCTCTG CATTAAGTGC AAAAGTTATC ATTAACATAT TAAGAACAGC AAACATTAAC AACATTATAT TCATTGATCT ACATTCAAAT CAATTATCTG GTTTCTTTGA CATAGCAACT ACTAATTTAA GTTCCCACAC TGTTTTCATA GGAGATATTA TAGAAAAACA TAATACTAAC AACTTAGTTG TTGTATCTCC TGACTATGGA GCATTAAACA GAACTCGAAC TTTTGCAAAC GTATTATCAA AACAGTGCAA ATTACACAAC GAAATTCAAG TAGCAGTAAT AGATAAATAC AGAGCAAAAC CTGGTGTATC TGAAGTAATG AACATAACTG GTAATGTAGA AAACAAAGAT TGTATAATAG TAGATGATAT TGTAGATTCA GCTGGAACAT TATGCAATGC GGCATCAGCT TTAAAAGACA GGGGAGCTTT ACAAGTTAGT GCATATGTTA CACATGGAAT ATTATCTGGC AATGCAGTAG AAAAAGTTAC GAATTCAAAA CTTGATAATT TAATAATAAC AGACACAATA CATAATGGTT TCTTCAACAC ATCAAAAATC CGAATTTTAT CCATTGATAA ATTTTTAAGC AACTATATAC TGTATAATTT AGTA
Details for EhchA.01131.a.A1.GE27398
HARVESTED ON: 9/15/2009
SEQUENCED ON: 10/20/2009
EXPECTED MW: 37kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMLISSGT SSINLAKCIS NITSIKLVNS CISRFSDQEL NVEIQENQDD KNKHVIIINS LCSPAHDNLL ELLLLTDAIN RTLHPRKITM IIPYLCYTRQ DRVMYRNLDD NSLMVSALSA KVIINILRTA NINNIIFIDL HSNQLSGFFD IATTNLSSHT VFIGDIIEKH NTNNLVVVSP DYGALNRTRT FANVLSKQCK LHNEIQVAVI DKYRAKPGVS EVMNITGNVE NKDCIIVDDI VDSAGTLCNA ASALKDRGAL QVSAYVTHGI LSGNAVEKVT NSKLDNLIIT DTIHNGFFNT SKIRILSIDK FLSNYILYNL V
Validated NT Sequence
ttattatact aaattataca gtatatagtt gcttaaaaat ttatcaatgg ataaaattcg gatttttgat gtgttgaaga aaccattatg tattgtgtct gttattatta aattatcaag ttttgaattc gtaacttttt ctactgcatt gccagataat attccatgtg taacatatgc actaacttgt aaagctcccc tgtcttttaa agctgatgcc gcattgcata atgttccagc tgaatctaca atatcatcta ctattataca atctttgttt tctacattac cagttatgtt cattacttca gatacaccag gttttgctct gtatttatct attactgcta cttgaatttc gttgtgtaat ttgcactgtt ttgataatac gtttgcaaaa gttcgagttc tgtttaatgc tccatagtca ggagatacaa caactaagtt gttagtatta tgtttttcta taatatctcc tatgaaaaca gtgtgggaac ttaaattagt agttgctatg tcaaagaaac cagataattg atttgaatgt agatcaatga atataatgtt gttaatgttt gctgttctta atatgttaat gataactttt gcacttaatg cagagaccat taggctgttg tcatctaagt ttctatacat tactctgtct tgtctagtgt aacaaagata aggtattatc atggttattt ttctaggatg caatgttcta ttaattgcat ccgttaacaa gagtaattct agtaaattat catgtgctgg ggaacaaagt gaatttataa ttatgacgtg tttatttttg tcatcttggt tttcttgaat ttctacgttt aattcttgat cagaaaaacg agaaatacaa gagttaacta actttatact tgtaatgttt gatatgcact ttgctaagtt aatactagat gtaccagaag atattaacat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLISSGTSS INLAKCISNI TSIKLVNSCI SRFSDQELNV EIQENQDDKN KHVIIINSLC SPAHDNLLEL LLLTDAINRT LHPRKITMII PYLCYTRQDR VMYRNLDDNS LMVSALSAKV IINILRTANI NNIIFIDLHS NQLSGFFDIA TTNLSSHTVF IGDIIEKHNT NNLVVVSPDY GALNRTRTFA NVLSKQCKLH NEIQVAVIDK YRAKPGVSEV MNITGNVENK DCIIVDDIVD SAGTLCNAAS ALKDRGALQV SAYVTHGILS GNAVEKVTNS KLDNLIITDT IHNGFFNTSK IRILSIDKFL SNYILYNLV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttaatatct tctggtacat ctagtattaa cttagcaaag tgcatatcaa acattacaag tataaagtta gttaactctt gtatttctcg tttttctgat caagaattaa acgtagaaat tcaagaaaac caagatgaca aaaataaaca cgtcataatt ataaattcac tttgttcccc agcacatgat aatttactag aattactctt gttaacggat gcaattaata gaacattgca tcctagaaaa ataaccatga taatacctta tctttgttac actagacaag acagagtaat gtatagaaac ttagatgaca acagcctaat ggtctctgca ttaagtgcaa aagttatcat taacatatta agaacagcaa acattaacaa cattatattc attgatctac attcaaatca attatctggt ttctttgaca tagcaactac taatttaagt tcccacactg ttttcatagg agatattata gaaaaacata atactaacaa cttagttgtt gtatctcctg actatggagc attaaacaga actcgaactt ttgcaaacgt attatcaaaa cagtgcaaat tacacaacga aattcaagta gcagtaatag ataaatacag agcaaaacct ggtgtatctg aagtaatgaa cataactggt aatgtagaaa acaaagattg tataatagta gatgatattg tagattcagc tggaacatta tgcaatgcgg catcagcttt aaaagacagg ggagctttac aagttagtgc atatgttaca catggaatat tatctggcaa tgcagtagaa aaagttacga attcaaaact tgataattta ataataacag acacaataca taatggtttc ttcaacacat caaaaatccg aattttatcc attgataaat ttttaagcaa ctatatactg tataatttag taaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa