EhchA.01108.a

phosphate ABC transporter, ATP-binding protein

CENTER ID: EhchA.01108.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01108.a.A1.GE27417 Full length( EhchA.01108.a ) 1 253
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.379
RefSeq: YP_507262.1
UniProt: Q2GH21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQRIHASAR NLSLWYDTNQ ILYDINLDVY KREVTAFIGP SGCGKSTFLR CFNRMNDFIS NCKIKGSLNV DDIGVYSVNT NVVLLRAKVG MVFQKPNPFP KSIYDNVAYG PKLHGLAKNK KKLDEIVEKS LTSVGLWEEL CDRLKDNAFE LSGGQQQRLC IARAIAVRPT MLLMDEPCSA LDPVATNVIE NLIQNLKKNF TIIVVTHSMK QAKKISDRVA VFDSGRIVEY NTTQEIFQNP QSSKTQRYIV DQL
NT Sequence
ATGTCACAAC GAATTCATGC TTCAGCTAGA AATTTAAGTT TATGGTATGA TACTAACCAA ATTTTATATG ATATAAATTT GGATGTATAT AAAAGAGAAG TTACAGCTTT TATAGGTCCT TCAGGATGTG GTAAATCTAC TTTCTTGAGA TGTTTTAATC GTATGAATGA TTTTATATCA AATTGTAAAA TTAAGGGTAG TTTAAATGTT GATGACATAG GTGTTTATTC TGTTAATACG AATGTGGTTC TTCTTCGTGC AAAAGTTGGT ATGGTTTTTC AAAAACCTAA TCCTTTTCCC AAGTCTATAT ATGATAATGT TGCTTATGGT CCAAAACTAC ACGGGTTGGC TAAGAATAAG AAAAAGTTAG ATGAAATTGT AGAAAAAAGC TTAACAAGTG TTGGTTTGTG GGAAGAGTTA TGTGATAGAT TAAAAGATAA TGCTTTTGAA TTATCTGGTG GACAACAGCA GCGCTTATGT ATAGCGCGTG CTATTGCAGT GAGGCCAACT ATGTTACTTA TGGATGAACC ATGTTCTGCT CTTGATCCTG TTGCTACAAA TGTAATTGAG AATTTAATAC AAAACTTAAA GAAAAATTTT ACCATTATTG TAGTAACTCA TTCTATGAAG CAAGCTAAGA AAATATCTGA TAGAGTAGCA GTTTTTGATT CTGGAAGAAT AGTTGAATAT AATACTACTC AAGAGATTTT TCAAAATCCA CAGTCAAGTA AAACTCAGAG GTATATAGTA GATCAGTTA
Details for EhchA.01108.a.A1.GE27417
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 30kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSQRIHASA RNLSLWYDTN QILYDINLDV YKREVTAFIG PSGCGKSTFL RCFNRMNDFI SNCKIKGSLN VDDIGVYSVN TNVVLLRAKV GMVFQKPNPF PKSIYDNVAY GPKLHGLAKN KKKLDEIVEK SLTSVGLWEE LCDRLKDNAF ELSGGQQQRL CIARAIAVRP TMLLMDEPCS ALDPVATNVI ENLIQNLKKN FTIIVVTHSM KQAKKISDRV AVFDSGRIVE YNTTQEIFQN PQSSKTQRYI V
Validated NT Sequence
atctactata tacctctgag ttttacttga ctgtggattt tgaaaaatct cttgagtagt attatattca actattcttc cagaatcaaa aactgctact ctatcagata ttttcttagc ttgcttcata gaatgagtta ctacaataat ggtaaaattt ttctttaagt tttgtattaa attctcaatt acatttgtag caacaggatc aagagcagaa catggttcat ccataagtaa catagttggc ctcactgcaa tagcacgcgc tatacataag cgctgctgtt gtccaccaga taattcaaaa gcattatctt ttaatctatc acataactct tcccacaaac caacacttgt taagcttttt tctacaattt catctaactt tttcttattc ttagccaacc cgtgtagttt tggaccataa gcaacattat catatataga cttgggaaaa ggattaggtt tttgaaaaac cataccaact tttgcacgaa gaagaaccac attcgtatta acagaataaa cacctatgtc atcaacattt aaactaccct taattttaca atttgatata aaatcattca tacgattaaa acatctcaag aaagtagatt taccacatcc tgaaggacct ataaaagctg taacttctct tttatataca tccaaattta tatcatataa aatttggtta gtatcatacc ataaacttaa atttctagct gaagcatgaa ttcgttgtga catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSQRIHASA RNLSLWYDTN QILYDINLDV YKREVTAFIG PSGCGKSTFL RCFNRMNDFI SNCKIKGSLN VDDIGVYSVN TNVVLLRAKV GMVFQKPNPF PKSIYDNVAY GPKLHGLAKN KKKLDEIVEK SLTSVGLWEE LCDRLKDNAF ELSGGQQQRL CIARAIAVRP TMLLMDEPCS ALDPVATNVI ENLIQNLKKN FTIIVVTHSM KQAKKISDRV AVFDSGRIVE YNTTQEIFQN PQSSKTQRYI VDQL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcacaacga attcatgctt cagctagaaa tttaagttta tggtatgata ctaaccaaat tttatatgat ataaatttgg atgtatataa aagagaagtt acagctttta taggtccttc aggatgtggt aaatctactt tcttgagatg ttttaatcgt atgaatgatt ttatatcaaa ttgtaaaatt aagggtagtt taaatgttga tgacataggt gtttattctg ttaatacgaa tgtggttctt cttcgtgcaa aagttggtat ggtttttcaa aaacctaatc cttttcccaa gtctatatat gataatgttg cttatggtcc aaaactacac gggttggcta agaataagaa aaagttagat gaaattgtag aaaaaagctt aacaagtgtt ggtttgtggg aagagttatg tgatagatta aaagataatg cttttgaatt atctggtgga caacagcagc gcttatgtat agcgcgtgct attgcagtga ggccaactat gttacttatg gatgaaccat gttctgctct tgatcctgtt gctacaaatg taattgagaa tttaatacaa aacttaaaga aaaattttac cattattgta gtaactcatt ctatgaagca agctaagaaa atatctgata gagtagcagt ttttgattct ggaagaatag ttgaatataa tactactcaa gagatttttc aaaatccaca gtcaagtaaa actcagaggt atatagtaga tcagttaaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa