EhchA.01074.a
nicotinate-nucleotide pyrophosphorylase
| CENTER ID: | EhchA.01074.a |
| ORGANISM: | Ehrlichia chaffeensis str. Arkansas |
| ASSOCIATED DISEASE: | Human monocytic ehrlichiosis |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
I |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EhchA.01074.a.A1.GE27396 | Full length( EhchA.01074.a ) | 1 | 279 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| EhchA.01074.a.A1.PW27389 | Full length( EhchA.01074.a ) | 1 | 279 |
Structures
3L0G
DEPOSITED: 12/9/2009
DETERMINATION: XRay
CLONE: EhchA.01074.a.A1.GE27396
PROTEIN: EhchA.01074.a.A1.PW27389
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_128545 |
| PATRIC ID: | fig|205920.11.peg.24 |
| RefSeq: | YP_506859.1 |
| UniProt: | Q2GI74 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKISFSEIIH NALKEDLGDK GDITTNSILI NEKVNFAINT RENLVVCGIP ILEEVFNMNK EHVKYEIHKK DGDITGKNST LVSGEALAIY LLPIERVILN FIQHASGIAS ITRQFVDEVS GTKVKIRSTR KTTPGLRMLD KYSVCIGGGE SYRDNLCDGV LIKDNHIASC GSITLAIQRL RKNLKNEYIA IECDNISQVE ESLSNNVDMI LLDNMSISEI KKAVDIVNGK SVLEVSGCVN IRNVRNIALT GVDYISIGCI TNSFQNKDIG LDIEYNNNK
NT Sequence
ATGAAAATCT CATTTTCAGA AATTATACAT AATGCATTAA AAGAAGATTT AGGAGACAAA GGTGATATAA CTACTAATAG TATATTAATT AATGAAAAAG TAAATTTTGC AATAAATACA AGAGAAAATT TAGTAGTTTG TGGAATTCCT ATACTAGAAG AAGTCTTTAA TATGAATAAA GAGCATGTAA AGTATGAAAT TCATAAGAAA GATGGAGATA TAACAGGAAA AAATAGCACC TTAGTAAGTG GAGAAGCATT AGCAATATAT TTACTGCCTA TTGAAAGAGT TATTTTAAAC TTCATTCAGC ATGCTTCTGG TATAGCGTCA ATCACTAGGC AATTTGTCGA TGAAGTATCT GGAACAAAAG TTAAGATTCG TAGCACTAGA AAAACTACTC CTGGTTTAAG AATGTTAGAT AAATATTCTG TATGTATTGG TGGTGGGGAA AGTTATAGAG ACAATTTGTG TGATGGTGTA CTTATAAAAG ATAATCATAT AGCTAGCTGT GGAAGTATTA CCTTAGCTAT ACAAAGGCTT AGAAAAAATC TAAAAAATGA ATATATTGCT ATTGAATGTG ACAATATATC TCAAGTTGAG GAATCACTGT CTAATAATGT AGATATGATA TTGTTAGATA ATATGAGTAT TAGTGAAATA AAAAAAGCGG TTGATATAGT TAATGGAAAA TCAGTTTTAG AAGTTTCTGG ATGTGTGAAC ATAAGAAATG TCAGAAACAT TGCACTAACA GGAGTAGATT ATATTTCTAT TGGGTGTATA ACTAACTCTT TTCAAAATAA AGATATAGGA CTTGACATAG AGTACAACAA TAATAAG
Details for EhchA.01074.a.A1.GE27396
| HARVESTED ON: | 9/15/2009 |
| SEQUENCED ON: | 9/30/2009 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with sequence variation |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKISFSEII HNALKEDLGD KGDITTNSIL INEKVNFAIN TRENLVVCGI PILEEVFNMN KEHVKYEIHK KDGDITGKNS TLVSGEALAI YLLPIERVIL NFIQHASGIA SITRQFVDEV SGTKVKIRST RKTTPGLRML DKYSVCIGGG ESYRDNLCDG VLIKDNHIAS CGSITLAIQR LRKNLKNEYI AIECDNISQV EESLSNNVDM ILLDNMSISE IKKAVDIVNG KSVLEVSGCV NIRNVRNIAL TGVDYISIGC ITNSFQNKDI GLDIEYNN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaaa tctcattttc agaaattata cataatgcat taaaagaaga tttaggagac aaaggtgata taactactaa tagtatatta attaatgaaa aagtaaattt tgcaataaat acaagagaaa atttagtagt ttgtggaatt cctatactag aagaagtctt taatatgaat aaagagcatg taaagtatga aattcacaag aaagatggag atataacagg aaaaaatagc accttagtaa gtggagaagc attagcaata tatttactgc ctattgaaag agttatttta aacttcattc agcatgcttc tggtatagcg tcaatcacta ggcaatttgt cgatgaagta tctggaacaa aagttaagat tcgtagcact agaaaaacta ctcctggttt aagaatgtta gataaatatt ctgtatgtat tggtggtggg gaaagttata gagacaattt gtgtgatggt gtacttataa aagataatca tatagctagc tgtggaagta ttaccttagc tatacaaagg cttagaaaaa atctaaaaaa tgaatatatt gctattgaat gtgacaatat atctcaagtt gaggaatcac tgtctaataa tgtagatatg atattgttag ataatatgag tattagtgaa ataaaaaaag cggttgatat agttaatgga aaatcagttt tagaagtttc tggatgtgtg aacataagaa atgtcagaaa cattgcacta acaggagtag attatatttc tattgggtgt ataactaact cttttcaaaa taaagatata ggacttgaca tagagtacaa caata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKISFSEII HNALKEDLGD KGDITTNSIL INEKVNFAIN TRENLVVCGI PILEEVFNMN KEHVKYEIHK KDGDITGKNS TLVSGEALAI YLLPIERVIL NFIQHASGIA SITRQFVDEV SGTKVKIRST RKTTPGLRML DKYSVCIGGG ESYRDNLCDG VLIKDNHIAS CGSITLAIQR LRKNLKNEYI AIECDNISQV EESLSNNVDM ILLDNMSISE IKKAVDIVNG KSVLEVSGCV NIRNVRNIAL TGVDYISIGC ITNSFQNKDI GLDIEYNNNK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaaatctca ttttcagaaa ttatacataa tgcattaaaa gaagatttag gagacaaagg tgatataact actaatagta tattaattaa tgaaaaagta aattttgcaa taaatacaag agaaaattta gtagtttgtg gaattcctat actagaagaa gtctttaata tgaataaaga gcatgtaaag tatgaaattc ataagaaaga tggagatata acaggaaaaa atagcacctt agtaagtgga gaagcattag caatatattt actgcctatt gaaagagtta ttttaaactt cattcagcat gcttctggta tagcgtcaat cactaggcaa tttgtcgatg aagtatctgg aacaaaagtt aagattcgta gcactagaaa aactactcct ggtttaagaa tgttagataa atattctgta tgtattggtg gtggggaaag ttatagagac aatttgtgtg atggtgtact tataaaagat aatcatatag ctagctgtgg aagtattacc ttagctatac aaaggcttag aaaaaatcta aaaaatgaat atattgctat tgaatgtgac aatatatctc aagttgagga atcactgtct aataatgtag atatgatatt gttagataat atgagtatta gtgaaataaa aaaagcggtt gatatagtta atggaaaatc agttttagaa gtttctggat gtgtgaacat aagaaatgtc agaaacattg cactaacagg agtagattat atttctattg ggtgtataac taactctttt caaaataaag atataggact tgacatagag tacaacaata ataagaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for EhchA.01074.a.A1.PW27389
| PURIFICATION DATe: | 10/5/2009 |
| CONCENTRATION: | 29.33mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 17 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKISFSEII HNALKEDLGD KGDITTNSIL INEKVNFAIN TRENLVVCGI PILEEVFNMN KEHVKYEIHK KDGDITGKNS TLVSGEALAI YLLPIERVIL NFIQHASGIA SITRQFVDEV SGTKVKIRST RKTTPGLRML DKYSVCIGGG ESYRDNLCDG VLIKDNHIAS CGSITLAIQR LRKNLKNEYI AIECDNISQV EESLSNNVDM ILLDNMSISE IKKAVDIVNG KSVLEVSGCV NIRNVRNIAL TGVDYISIGC ITNSFQNKDI GLDIEYNN
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaaaa tctcattttc agaaattata cataatgcat taaaagaaga tttaggagac aaaggtgata taactactaa tagtatatta attaatgaaa aagtaaattt tgcaataaat acaagagaaa atttagtagt ttgtggaatt cctatactag aagaagtctt taatatgaat aaagagcatg taaagtatga aattcacaag aaagatggag atataacagg aaaaaatagc accttagtaa gtggagaagc attagcaata tatttactgc ctattgaaag agttatttta aacttcattc agcatgcttc tggtatagcg tcaatcacta ggcaatttgt cgatgaagta tctggaacaa aagttaagat tcgtagcact agaaaaacta ctcctggttt aagaatgtta gataaatatt ctgtatgtat tggtggtggg gaaagttata gagacaattt gtgtgatggt gtacttataa aagataatca tatagctagc tgtggaagta ttaccttagc tatacaaagg cttagaaaaa atctaaaaaa tgaatatatt gctattgaat gtgacaatat atctcaagtt gaggaatcac tgtctaataa tgtagatatg atattgttag ataatatgag tattagtgaa ataaaaaaag cggttgatat agttaatgga aaatcagttt tagaagtttc tggatgtgtg aacataagaa atgtcagaaa cattgcacta acaggagtag attatatttc tattgggtgt ataactaact cttttcaaaa taaagatata ggacttgaca tagagtacaa caata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKISFSEII HNALKEDLGD KGDITTNSIL INEKVNFAIN TRENLVVCGI PILEEVFNMN KEHVKYEIHK KDGDITGKNS TLVSGEALAI YLLPIERVIL NFIQHASGIA SITRQFVDEV SGTKVKIRST RKTTPGLRML DKYSVCIGGG ESYRDNLCDG VLIKDNHIAS CGSITLAIQR LRKNLKNEYI AIECDNISQV EESLSNNVDM ILLDNMSISE IKKAVDIVNG KSVLEVSGCV NIRNVRNIAL TGVDYISIGC ITNSFQNKDI GLDIEYNNNK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAAATCTCA TTTTCAGAAA TTATACATAA TGCATTAAAA GAAGATTTAG GAGACAAAGG TGATATAACT ACTAATAGTA TATTAATTAA TGAAAAAGTA AATTTTGCAA TAAATACAAG AGAAAATTTA GTAGTTTGTG GAATTCCTAT ACTAGAAGAA GTCTTTAATA TGAATAAAGA GCATGTAAAG TATGAAATTC ATAAGAAAGA TGGAGATATA ACAGGAAAAA ATAGCACCTT AGTAAGTGGA GAAGCATTAG CAATATATTT ACTGCCTATT GAAAGAGTTA TTTTAAACTT CATTCAGCAT GCTTCTGGTA TAGCGTCAAT CACTAGGCAA TTTGTCGATG AAGTATCTGG AACAAAAGTT AAGATTCGTA GCACTAGAAA AACTACTCCT GGTTTAAGAA TGTTAGATAA ATATTCTGTA TGTATTGGTG GTGGGGAAAG TTATAGAGAC AATTTGTGTG ATGGTGTACT TATAAAAGAT AATCATATAG CTAGCTGTGG AAGTATTACC TTAGCTATAC AAAGGCTTAG AAAAAATCTA AAAAATGAAT ATATTGCTAT TGAATGTGAC AATATATCTC AAGTTGAGGA ATCACTGTCT AATAATGTAG ATATGATATT GTTAGATAAT ATGAGTATTA GTGAAATAAA AAAAGCGGTT GATATAGTTA ATGGAAAATC AGTTTTAGAA GTTTCTGGAT GTGTGAACAT AAGAAATGTC AGAAACATTG CACTAACAGG AGTAGATTAT ATTTCTATTG GGTGTATAAC TAACTCTTTT CAAAATAAAG ATATAGGACT TGACATAGAG TACAACAATA ATAAGAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA