EhchA.01072.a

cytidylate kinase

CENTER ID: EhchA.01072.a
ORGANISM: Ehrlichia chaffeensis str. Arkansas
ASSOCIATED DISEASE: Human monocytic ehrlichiosis
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: unclassified
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
EhchA.01072.a.A1.GE27395 Full length( EhchA.01072.a ) 1 213
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|205920.11.peg.342
RefSeq: YP_507223.1
UniProt: Q2GH60

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKHCNNFVI TIDGPSASGK GSLAKKLANF LNFQYLDTGK LYRIIATIFG TSAQNHLKID NKLEDKILSL LLEDNNTIYN NEDIGKTASI LARYQHIRLA LLPIQRKLIH LAEPGIILDG RDTSSTVFPE ADLKIFITAQ ASIRAQRRFK EMLYHKKEMY KKIHKQILER DIRDTYRKFS PLVKVKDALY INTSYMNKEQ VLLYIKEKIK TYL
NT Sequence
ATGACCAAGC ATTGCAATAA TTTCGTTATA ACAATTGATG GTCCTTCTGC TTCAGGAAAA GGAAGTTTAG CAAAAAAATT AGCAAATTTT CTTAACTTTC AGTATTTAGA TACAGGAAAA TTATACAGGA TAATAGCTAC AATATTTGGA ACATCAGCAC AAAACCATCT AAAAATTGAC AATAAATTAG AGGACAAAAT ATTATCACTG CTCTTAGAAG ATAATAATAC TATATACAAT AATGAAGACA TAGGCAAAAC TGCTTCAATT TTAGCAAGAT ATCAACACAT TAGACTTGCA TTATTACCTA TACAGCGCAA ACTTATCCAT CTAGCAGAAC CAGGTATAAT CCTAGATGGC AGAGATACAT CTTCTACAGT ATTTCCTGAA GCAGACCTTA AAATTTTTAT TACAGCACAA GCATCTATTA GAGCACAGCG AAGGTTTAAA GAAATGTTAT ATCACAAGAA AGAAATGTAT AAAAAAATAC ACAAACAAAT ACTAGAAAGG GATATTAGAG ATACCTATAG AAAATTTTCT CCTTTGGTAA AAGTAAAAGA TGCCCTATAC ATTAACACTT CATACATGAA TAAAGAACAG GTACTTTTAT ACATTAAAGA AAAAATAAAA ACCTACTTG
Details for EhchA.01072.a.A1.GE27395
HARVESTED ON: 9/15/2009
SEQUENCED ON: 9/30/2009
EXPECTED MW: 26kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTKHCNNFV ITIDGPSASG KGSLAKKLAN FLNFQYLDTG KLYRIIATIF GTSAQNHLKI DNKLEDKILS LLLEDNNTIY NNEDIGKTAS ILARYQHIRL ALLPIQRKLI HLAEPGIILD GRDTSSTVFP EADLKIFITA QASIRAQRRF KEMLYHKKEM YKKIHKQILE RDIRDTYRKF SPLVKVKDAL YINTSYMNKE QVLLYIKEKI KT
Validated NT Sequence
aggtttttat tttttcttta atgtataaaa gtacctgttc tttattcatg tatgaagtgt taatgtatag ggcatctttt acttttacca aaggagaaaa ttttctatag gtatctctaa tatccctttc tagtatttgt ttgtgtattt ttttatacat ttctttcttg tgatataaca tttctttaaa ccttcgctgt gctctaatag atgcttgtgc tgtaataaaa attttaaggt ctgcttcagg aaatactgta gaagatgtat ctctgccatc taggattata cctggttctg ctagatggat aagtttgcgc tgtataggta ataatgcaag tctaatgtgt tgatatcttg ctaaaattga agcagttttg cctatgtctt cattattgta tatagtatta ttatcttcta agagcagtga taatattttg tcctctaatt tattgtcaat ttttagatgg ttttgtgctg atgttccaaa tattgtagct attatcctgt ataattttcc tgtatctaaa tactgaaagt taagaaaatt tgctaatttt tttgctaaac ttccttttcc tgaagcagaa ggaccatcaa ttgttataac gaaattattg caatgcttgg tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTKHCNNFV ITIDGPSASG KGSLAKKLAN FLNFQYLDTG KLYRIIATIF GTSAQNHLKI DNKLEDKILS LLLEDNNTIY NNEDIGKTAS ILARYQHIRL ALLPIQRKLI HLAEPGIILD GRDTSSTVFP EADLKIFITA QASIRAQRRF KEMLYHKKEM YKKIHKQILE RDIRDTYRKF SPLVKVKDAL YINTSYMNKE QVLLYIKEKI KTYL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccaagcat tgcaataatt tcgttataac aattgatggt ccttctgctt caggaaaagg aagtttagca aaaaaattag caaattttct taactttcag tatttagata caggaaaatt atacaggata atagctacaa tatttggaac atcagcacaa aaccatctaa aaattgacaa taaattagag gacaaaatat tatcactgct cttagaagat aataatacta tatacaataa tgaagacata ggcaaaactg cttcaatttt agcaagatat caacacatta gacttgcatt attacctata cagcgcaaac ttatccatct agcagaacca ggtataatcc tagatggcag agatacatct tctacagtat ttcctgaagc agaccttaaa atttttatta cagcacaagc atctattaga gcacagcgaa ggtttaaaga aatgttatat cacaagaaag aaatgtataa aaaaatacac aaacaaatac tagaaaggga tattagagat acctatagaa aattttctcc tttggtaaaa gtaaaagatg ccctatacat taacacttca tacatgaata aagaacaggt acttttatac attaaagaaa aaataaaaac ctacttgaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa